scholarly journals Particle and Gel Characterization of Irinotecan-Loaded Double-Reverse Thermosensitive Hydrogel

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 551
Author(s):  
Fakhar ud Din ◽  
Sung Giu Jin ◽  
Han-Gon Choi
Keyword(s):  
Entrapment Efficiency ◽  
Tween 80 ◽  
Rectal Administration ◽  
The Body ◽  
Poloxamer 407 ◽  
Gel Properties ◽  
Thermosensitive Hydrogel ◽  
Lipid Mixture ◽  
Dispersed System

The irinotecan-loaded double-reverse thermosensitive hydrogel (DRTH) is a dispersed system of irinotecan-loaded solid lipid nanoparticles (SLN) in a thermosensitive hydrogel. To optimise the particle and gel properties of DRTHs for rectal administration of irinotecan, SLNs and DRTHs were prepared with tricaprin, triethanolamine, Tween 80, and Span 20. Among the SLNs tested, an SLN composed of 1 g irinotecan, 0.5 g lipid mixture, and 0.5 g combined surfactant gave the highest entrapment efficiency and smallest particle size. A DRTH composed of (poloxamer 407/poloxamer 188/combined surfactant/SLN dispersion/H2O (10/15/17/4/54%)) showed easy administration, fast gelling, and strong gel-forming in the body.

scholarly journals Thermosensitive Hydrogel for Encapsulation and Controlled Release of Biocontrol Agents to Prevent Peanut Aflatoxin Contamination

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 547 ◽  
Author(s):  
Jiachang Feng ◽  
Jianpeng Dou ◽  
Youzhen Zhang ◽  
Zidan Wu ◽  
Dongxue Yin ◽  
...  
Keyword(s):  
Entrapment Efficiency ◽  
Honeycomb Structure ◽  
Aflatoxin Contamination ◽  
Biocontrol Agents ◽  
Solution Temperature ◽  
Temperature Sensitive ◽  
Interpenetrating Network ◽  
Thermosensitive Hydrogel ◽  
Hydrogel Beads

Starch, alginate, and poly(N-isopropylacrylamide) (PNIPAAm) were combined to prepare a semi-interpenetrating network (IPN) hydrogel with temperature sensitivity. Calcium chloride was used as cross-linking agent, the non-toxigenic Aspergillus flavus spores were successfully encapsulated as biocontrol agents by the method of ionic gelation. Characterization of the hydrogel was performed by Fourier-transform infrared spectroscopy (FTIR), scanning electron micrograph (SEM), and thermogravimetry analysis (TGA). Formulation characteristics, such as entrapment efficiency, beads size, swelling behavior, and rheological properties were evaluated. The optical and rheological measurements indicated that the lower critical solution temperature (LCST) of the samples was about 29–30 °C. TGA results demonstrated that the addition of kaolin could improve the thermal stability of the semi-IPN hydrogel. Morphological analysis showed a porous honeycomb structure on the surface of the beads. According to the release properties of the beads, the semi-IPN hydrogel beads containing kaolin not only have the effect of slow release before peanut flowering, but they also can rapidly release biocontrol agents after flowering begins. The early flowering stage of the peanut is the critical moment to apply biocontrol agents. Temperature-sensitive hydrogel beads containing kaolin could be considered as carriers of biocontrol agents for the control of aflatoxin in peanuts.

scholarly journals Anastrozole Loaded Nanostructured Lipid Carriers : Preparation and Evaluation

2021 ◽  
Vol 30 (2) ◽  
pp. 185-195
Author(s):  
Alaa Abdul-Jabbar Hashim ◽  
Nawal A. Rajab
Keyword(s):  
Hot Flashes ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Nanostructured Lipid Carriers ◽  
Poloxamer 407 ◽  
Fourth Generation ◽  
Glycol Succinate ◽  
Release Mechanism ◽  
Optimum Formula

Anastrozole (ANZ) is considered constitute of the fourth –generation of Non–steroidal aromatase blockage, ANZ has use for hormone receptor positive breast cancer in postmenopausal women. The serious side effects of ANZ including, vaginal dryness, hot flashes, irritability, breast tenderness and un–stability in circulation. Nanostructured lipid carriers (NLCs) have recently emerged as a multifunctional platform for drug delivery in cancer therapy. Five formula were composed of (200 mg of glyceryl monostearate, 40 mg of oleic acid , 1% (w/w) Tween 80, 1% (w/w) Poloxamer 407, 1% (w/w) soy lecithin and Vitamin E Polyethylene Glycol Succinate. The mean particle size, polydispersity index, zeta potential, entrapment efficiency, loading capacity range of optimum formula F05 (166±3.86 nm), (0.271±0.04), (–23.7±2.65 mV), (42.43±3.90%) and (1.23±0.35%) respectively that prepared by same above composition but higher amplitude value (70%). The in–vitro drug leakage study demonstrated intact formula through 5 hours, with an approximately 78.37% of the drug was encapsulated, that exhibit an anomalous release mechanism.

scholarly journals OPTIMIZATION OF LUTEOLIN-LOADED TRANSFERSOME USING RESPONSE SURFACE METHODOLOGY

2017 ◽  
Vol 9 ◽  
pp. 107
Author(s):  
Damai Ria Setyawati ◽  
Silvia Surini ◽  
Etik Mardliyati
Keyword(s):  
Particle Size ◽  
Response Surface Methodology ◽  
Zeta Potential ◽  
Response Surface ◽  
Total Lipid ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Spherical Particles ◽  
Lipid Mixture ◽  
Independent Variables

Objective: This research was carried out to optimize luteolin-loaded transfersome formula with independent variables such as lipid–surfactant (totallipid) concentration and luteolin concentration.Methods: Luteolin-loaded transfersome was optimized by response surface methodology based on four parameters, namely, particle size (Z-average),polydispersity index, zeta potential, and entrapment efficiency. The transfersome formula was prepared using central composite design, and theselected independent variables were the total lipid (mixture of phospholipid and Tween 80) and luteolin concentrations. 14 formulas of luteolinloadedtransfersome were prepared by thin film hydration, followed by the sonication method.Results: The total lipid and luteolin concentration significantly affected the entrapment efficiency only. The other parameters were not affected by achange in these variables. The optimum formula of 4.88% total lipid and 0.5% luteolin with desirability value of 0.609 conformed with the predictionparameters. Vesicle imaging using transmission electron microscopy revealed spherical particles and the occurrence of particle aggregation. Theoptimum formula of luteolin-loaded transfersome possessed the following characteristics: Particle size of 286.03±8.46 nm, polydispersity indexof 0.480±0.013, zeta potential of -18.67±0.379 mV, and entrapment efficiency of 94.97±0.28 %. However, these values did not correspond to thepredicted values and were confirmed by the low adjusted and predicted R-squared values.Conclusion: This method can be applied to optimize the entrapment efficiency, and in the future, it can be used for further optimizing formula oftransfersome by including more variables.

scholarly journals NANOSTRUCTURED LIPID CARRIERS (NLCS): A PROMINENT TOPICAL DELIVERY SYSTEM FOR COENZYME Q10 AND MYRICA ESCULENTA LEAVES EXTRACT FOR ANTI-AGING POTENTIAL

2018 ◽  
Vol 10 (8) ◽  
pp. 106 ◽  
Author(s):  
Prashant Kumar ◽  
Anita Singh
Keyword(s):  
Coenzyme Q10 ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Poloxamer 407 ◽  
Injection Technique ◽  
23 Factorial Design ◽  
Myrica Esculenta ◽  
Formulation Variables

Objective: In this study, Myrica esculenta (ME) leaves extract along with Coenzyme Q10 was entrapped in nanostructured lipid carriers (NLCs). The formulation variables were successfully optimized using 23 factorial design.Methods: Preparation of NLC was carried out by solvent injection technique. The formulation of NLC was consisting of the methanolic extract of Myrica esculenta leaves and Coenzyme Q10 as key ingredients. Oleic Acid (OA) was taken as liquid lipid, Precirol ATO 5 (PATO) as solid lipid, and Poloxamer 407 (P407). The formulation variables were statically optimized using 23 factorial design, followed by the characterization of the formulation.Results: The results demonstrated that the best optimized NLC formulation F5 of Coenzyme Q10 and plant extract exhibited the smallest size 169.46 nm and highest entrapment efficiency 90.06±0.171 %. Furthermore, it showed good physical stability at 28 d after preparation.Conclusion: The developed NLCs of Q10-ME extract from this study could be explored as the effective anti-ageing formulation.

scholarly journals DEVELOPMENT AND CHARACTERIZATION OF SUSTAINED RELEASE METHOTREXATE LOADED CUBOSOMES FOR TOPICAL DELIVERY IN RHEUMATOID ARTHRITIS

2020 ◽  
pp. 33-39
Author(s):  
KARISHMA KAPOOR ◽  
VINAY PANDIT ◽  
UPENDRA NAGAICH
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Particle Size ◽  
Zeta Potential ◽  
Sustained Release ◽  
Drug Delivery System ◽  
Delivery System ◽  
Entrapment Efficiency ◽  
Poloxamer 407

Objective: Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are essential part of the administration of Rheumatoid Arthritis (RA). Methotrexate (MTX) is effective for tumor necrosis factor alpha (TNF-a) biologic agents, indicated only in minority of patients suffering from severe RA. MTX remains the "anchor drug" in the treatment of RA. For delivery improvement, novel pharmaceutical drug delivery system i.e. MTX-Cubosomes were developed. Methods: Poloxamer 407 and Glycerol monooleate (Monoelin, MO) used and the formulation were characterized as a sustained release drug delivery system for Methotrexate. Different ratios of Monolein, Poloxamer 407 and water were used to develop the different cubosomes using homogenization and emulsification method. Characterization of formulations for morphology was performed and also particle size distribution by Transmission Electron Microscopy (TEM). Results: Formulation showed the internal cubic structures of the vesicles. The particle size of the formulations was found to be ranging from 53.21 to 185.32 nm, zeta potential of the formulations varied from-18.20-36.10 mV. The cubosomal formulation exhibited good entrapment efficiency along with high drug loading. Compatibility with the excipients was also established. An in vitro release study was done using Franz Diffusion cell indicated sustained release of the formulation at a rate of 1.25 %/h. Cubosomes proved to be reliable system for sustained transdermal drug delivery system. Conclusion: Methotrexate cubosomes is a novel medication delivery framework and in this examination it has been developed and characterized. The formulations were found to be promising in terms of its characterization parameters like particle size, zeta potential, entrapment efficiency, loading capacity, release kinetics, and stability, suitable for topical delivery.

scholarly journals Characterization of Nanoemulsion Prepared from Self-emulsifying Rifampicin and its Antibacterial Effect on Staphylococcus aureus and Stap. epidermidis Isolated from Acne

2016 ◽  
Vol 14 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Kohinur Begum ◽  
Amit Sarker ◽  
Israt Jahan Shimu ◽  
Md Mazharul Islam Chowdhury ◽  
Reza Ul Jalil
Keyword(s):  
Antibacterial Effect ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Stability Study ◽  
Ph Stability ◽  
Particle Sizes ◽  
In Vitro Drug Release ◽  
Drug Content

In this study, three different self emulsifying drug delivery systems of rifampicin (SEDD-R) were made using oleic acid and different surfactants such as Tween 80, Chremophor RH 40 and Chremophor EL designated as RN-TW, RN-CRH and RN- CEL. These self-emulsifying systems were converted to rifampicin nanoemulsion by adding water under sonication. The resulting particle sizes were found to be 192.7 nm, 183.4 nm and 179.2 nm for RN- CEL, RN-CRH and RN-TW, respectively using Zetasizer. Drug content, entrapment efficiency, in vitro drug release and pH stability tests was performed. Drug content and entrapment efficiency for RNTW, RN-CRH and RN- CEL were found as 0.9945% & 0.9835%, 0.9720% & 84.31% and 74.58 % & 56.89%, respectively. The release of drug from RN-TW, RN-CRH and RN-CEL occurred for 450-, 330- and 240- minutes, respectively. The pH stability study demonstrated that all formulations retained maximum drug at pH 5.8. Antibacterial effect of the preparations was evaluated against S. epidermidis and S. aureus isolated from acne and showed effective results.Dhaka Univ. J. Pharm. Sci. 14(2): 171-177, 2015 (December)

Studies on the Characterization of Factor VIII and a Co-factor VIII

1974 ◽  
Vol 31 (02) ◽  
pp. 328-338
Author(s):  
M. M. P Paulssen ◽  
H. L. M. A Vandenbussche-Scheffers ◽  
P. B Spaan ◽  
T de Jong ◽  
M. C Planje
Keyword(s):  
Molecular Weight ◽  
Factor Viii ◽  
The Body ◽  
Haemophilia A ◽  
Von Willebrand's Disease ◽  
Von Willebrand’S Disease ◽  
Polysaccharide Content ◽  
Plasma Factor ◽  
Two Factors

SummaryFactor VIII occurs in the body in two different forms. In lymph factor VIII is bound to chylomicra. In plasma, factor VIII is bound to a protein.After delipidation of chylomicra we obtained a glycoprotein with a high polysaccharide content and a molecular weight of approx. 160,000.In plasma, factor VIII is attached to a protein which is present in normal concentrations in plasma of patients with haemophilia A and in serum (co-factor VIII).This factor is deficient in both the plasma and the serum of patients with von Willebrand’s disease.The binding between factor VIII and co-factor VIII is reversible.Some properties of these two factors are described.

The Sober Bacchae: Dance as Phenomenal Limitation in Nietzsche

2019 ◽  
Vol 37 (1) ◽  
pp. 59-76
Author(s):  
Monique Lyle
Keyword(s):  
World View ◽  
The Body ◽  
Specific Function ◽  
Alain Badiou ◽  
Classical Greece ◽  
Birth Of Tragedy

This essay seeks to dispel entrenched critical opinion regarding dance across Nietzsche's writings as representative of Dionysian intoxication alone. Taking as its prompt the riposte of Alain Badiou, ‘Nietzsche is miles away from any doctrine of dance as a primitive ecstasy’ and ‘dance is in no way the liberated bodily impulse, the wild energy of the body’, the essay uncovers the ties between dance and Apollo in the Nietzschean theory of art while qualifying dance's relation to Dionysus. Primarily through an analysis of The Dionysiac World View and The Birth of Tragedy, the essay seeks to illuminate enigmatic statements about dance in Nietzsche (‘in dance the greatest strength is only potential, although it is betrayed by the suppleness of movement’ and ‘dance is the preservation of orderly measure’). It does this through an elucidation of the specific function of dance in Nietzsche's interpretation of classical Greece; via an assessment of the difficulties associated with the Nietzschean understanding of the bacchanal; and lastly through an analysis of Nietzsche's characterization of dance as a symbol. The essay culminates in a discussion of dance's ties to Nietzschean life affirmation; here the themes of physico-phenomenal existence, joy and illusion in Nietzsche are surveyed.

In-vivo evaluation of cisplatin nanoparticles encompass natural polymer

2020 ◽  
Vol 6 (1) ◽  
pp. 1-7
Author(s):  
Bhavani J ◽  
Sunil Kumar Prajapati ◽  
Ravichandran S
Keyword(s):  
Tumor Volume ◽  
Nitrogen Mustard ◽  
Common Type ◽  
The Body ◽  
Nano Particles ◽  
Natural Polymer ◽  
Drinking Alcohol ◽  
In Vivo Evaluation

Cancer is assemblage diseases involving abnormal cell growth amid the potential of spread to other parts of the body due to tobacco use are the cause of about of cancer deaths. Another 10% is due to obesity, poor diet & drinking alcohol. In 2012 about 14.1 million new cases of cancer occurred globally. In females, the most common type is breast cancer. Cisplatin also known as cytophosphane is a nitrogen mustard alkylating agent from the oxazophosphinans groups were used to treat cancers & autoimmune disorders. Based on the above reasons I will fix the aim Preparation characterization of Cisplatin- nano particles  &  its anticancer activity. Solid tumor volume examination report showed that the assessment of different day indication 15,20,25 & 30th variations of different groups of tumor volumes were decreased CPG Nanoparticles (100 mg/kg)+ DAL(15th day 4.97±0.24↓), (20th day 0.6±0.13↓), (25th day 1.35±0.30↓) & (30th day 1.89±0.13↓).

scholarly journals Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Huan Li ◽  
Siruo Zhang ◽  
Ruina Liu ◽  
Lu Yuan ◽  
Di Wu ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Interval Estimation ◽  
The Body ◽  
Taxon Richness ◽  
Time Since Death ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Level Data ◽  
Species Taxon

AbstractOnce the body dies, the indigenous microbes of the host begin to break down the body from the inside and play a key role thereafter. This study aimed to investigate the probable shift in the composition of the rectal microbiota at different time intervals up to 15 days after death and to explore bacterial taxa important for estimating the time since death. At the phylum level, Proteobacteria and Firmicutes showed major shifts when checked at 11 different intervals and emerged at most of the postmortem intervals. At the species level, Enterococcus faecalis and Proteus mirabilis showed a downward and upward trend, respectively, after day 5 postmortem. The phylum-, family-, genus-, and species-taxon richness decreased initially and then increased considerably. The turning point occurred on day 9, when the genus, rather than the phylum, family, or species, provided the most information for estimating the time since death. We constructed a prediction model using genus-level data from high-throughput sequencing, and seven bacterial taxa, namely, Enterococcus, Proteus, Lactobacillus, unidentified Clostridiales, Vagococcus, unidentified Corynebacteriaceae, and unidentified Enterobacteriaceae, were included in this model. The abovementioned bacteria showed potential for estimating the shortest time since death.

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