An Overview on Collagen and Gelatin-Based Cryogels: Fabrication, Classification, Properties and Biomedical Applications

Decades of research into cryogels have resulted in the development of many types of cryogels for various applications. Collagen and gelatin possess nontoxicity, intrinsic gel-forming ability and physicochemical properties, and excellent biocompatibility and biodegradability, making them very desirable candidates for the fabrication of cryogels. Collagen-based cryogels (CBCs) and gelatin-based cryogels (GBCs) have been successfully applied as three-dimensional substrates for cell culture and have shown promise for biomedical use. A key point in the development of CBCs and GBCs is the quantitative and precise characterization of their properties and their correlation with preparation process and parameters, enabling these cryogels to be tuned to match engineering requirements. Great efforts have been devoted to fabricating these types of cryogels and exploring their potential biomedical application. However, to the best of our knowledge, no comprehensive overviews focused on CBCs and GBCs have been reported currently. In this review, we attempt to provide insight into the recent advances on such kinds of cryogels, including their fabrication methods and structural properties, as well as potential biomedical applications.

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Fabrication and characterization of egg white cryogel scaffold for three-dimensional (3D) cell culture

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2018.12.019 ◽

2019 ◽

Vol 17 ◽

pp. 441-446 ◽

Cited By ~ 3

Author(s):

Perumalsamy Balaji ◽

Anbazhagan Murugadas ◽

Sellathamby Shanmugaapriya ◽

Mohammad Abdulkader Akbarsha

Keyword(s):

Cell Culture ◽

Three Dimensional ◽

3D Cell Culture ◽

Egg White ◽

Fabrication And Characterization

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Characterization of covalent protein modification by triclosan in vivo and in vitro via three-dimensional liquid chromatography-mass spectrometry: New insight into its adverse effects

Environment International ◽

10.1016/j.envint.2019.105423 ◽

2020 ◽

Vol 136 ◽

pp. 105423 ◽

Cited By ~ 1

Author(s):

Meixian Liu ◽

Na Li ◽

Yida Zhang ◽

Zhiyuan Zheng ◽

Yue Zhuo ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Adverse Effects ◽

Protein Modification ◽

Three Dimensional ◽

Liquid Chromatography Mass Spectrometry ◽

Insight Into

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Production and the characterization of avian crypt-villus enteroids and the effect of chemicals

10.21203/rs.2.19481/v1 ◽

2019 ◽

Author(s):

Mohan Acharya ◽

Komala Arsi ◽

Annie Donoghue ◽

Rohana Liyanage ◽

Narayan C Rath

Keyword(s):

Cell Culture ◽

Intestinal Mucosa ◽

Culture Medium ◽

Disease Modeling ◽

Ex Vivo ◽

Mammalian Species ◽

Three Dimensional ◽

Gut Health ◽

Three Dimensional Models

Abstract Background: Three-dimensional models of cell culture such as organoids and mini organs accord better advantage over regular cell culture because of their ability to simulate organ functions hence, used for disease modeling, metabolic research, and the development of therapeutics strategies. However, most advances in this area are limited to mammalian species with little progress in others such as poultry where it can be deployed to study problems of agricultural importance. In the course of enterocyte culture in chicken, we observed that intestinal mucosal villus-crypts self-repair and lead to the formation of enteroid-like structures which appeared to be useful as ex vivo models to study enteric physiology and diseases. Results: The villus-crypts harvested from chicken intestinal mucosa were cultured to generate enteroids, purified by filtration then re cultured with different chemicals and growth factors to assess their response based on their morphological dispositions. Histochemical analyses using marker antibodies and probes showed the enteroids consisting different cells such as epithelial, goblet, and enteroendocrine cells typical to villi and retain functional characteristics of intestinal mucosa. Conclusions: The villus enteroids can simulate villus functions with their absorptive cells functionally positioned and exposed to culture medium thus, can help understand the mechanisms of nutrient uptake and their regulation, interactions with alien organisms, and amenable to assays of the factors that may affect gut health.

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Three-dimensional microstructure and numerical calculation of elastic properties of alpine snow with a focus on weak layers

Journal of Glaciology ◽

10.3189/2014jog13j220 ◽

2014 ◽

Vol 60 (222) ◽

pp. 705-713 ◽

Cited By ~ 17

Author(s):

Berna Köchle ◽

Martin Schneebeli

Keyword(s):

Young’S Modulus ◽

Young's Modulus ◽

Three Dimensional ◽

Large Change ◽

X Ray ◽

Weak Layer ◽

Snow Stratigraphy ◽

Precise Characterization ◽

Microstructural Variability

AbstractThe microstructure and stratigraphy of a snowpack determine its physical behaviour. Weak layers or weak interfaces buried under a slab are prerequisites for the formation of dry-snow slab avalanches, and a precise characterization of weak layers or interfaces is essential to assess stability. Yet their exact geometry and micromechanical properties are poorly known. We cast weak layers and their adjacent layers in the field during two winters and reconstructed their three-dimensional microstructure using X-ray microcomputer tomography. The high resolution of 10–20 μm allowed us to study snow stratigraphy at the microstructural scale. We quantified the microstructural variability for 32 centimetre-sized layered samples and we calculated Young’s modulus and Poisson’s ratio by tomography-based finite-element simulations. Layers in a sample could therefore be differentiated not only by a change in morphology or microstructure, but also by a change in mechanical properties. We found a logarithmic correlation of Young’s modulus with density for two different density ranges, consistent with previous studies. By calculating the relative microstructural changes within our samples, we showed that a large change could indicate a potential weak layer, but only when the weak layer and both adjacent layers, i.e. the sandwich, were considered.

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Design and characterization of a new bioreactor for continuous ultra-slow uniaxial distraction of a three-dimensional scaffold-free stem cell culture

Biotechnology Progress ◽

10.1002/btpr.510 ◽

2010 ◽

Vol 27 (1) ◽

pp. 86-94 ◽

Cited By ~ 4

Author(s):

S. Weiss ◽

P. Henle ◽

W. Roth ◽

R. Bock ◽

S. Boeuf ◽

...

Keyword(s):

Cell Culture ◽

Stem Cell ◽

Three Dimensional ◽

Stem Cell Culture

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Magnetic Microrobots: Fabrication and Characterization of Magnetic Microrobots for Three-Dimensional Cell Culture and Targeted Transportation (Adv. Mater. 41/2013)

Advanced Materials ◽

10.1002/adma.201370257 ◽

2013 ◽

Vol 25 (41) ◽

pp. 5829-5829 ◽

Cited By ~ 4

Author(s):

Sangwon Kim ◽

Famin Qiu ◽

Samhwan Kim ◽

Ali Ghanbari ◽

Cheil Moon ◽

...

Keyword(s):

Cell Culture ◽

Three Dimensional ◽

Fabrication And Characterization ◽

Magnetic Microrobots ◽

Dimensional Cell

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Three Dimensional Imaging of Microelectronic Devices Using a CrossBeam FIB

ISTFA 2004: Conference Proceedings from the 30th International Symposium for Testing and Failure Analysis ◽

10.31399/asm.cp.istfa2004p0429 ◽

2004 ◽

Author(s):

Eric Lifshin ◽

James Evertsen ◽

Edward Principe ◽

John Friel

Keyword(s):

High Resolution ◽

3D Imaging ◽

Ion Beam ◽

Three Dimensional ◽

Serial Sections ◽

Microelectronic Devices ◽

3D Microscopy ◽

Internal Structures ◽

Insight Into

Abstract Increased insight into the internal structure of microelectronic devices can be achieved through the use of three dimensional (3D) imaging based on image stacks of serial sections obtained with a combined electron and ion beam (CrossBeam) FIB. This study describes how such data can be collected and presented, some of the factors that need to be optimized to get the best images, and the limitations of the method. It can be viewed as a first step in the emerging area of high resolution 3D microscopy, a technique that can lead to more accurate characterization of the shapes of internal structures and their interconnectivity at the nanoscale.

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