scholarly journals Scaling-Up and Semi-Continuous Cultivation of Locally Isolated Marine Microalgae Tetraselmis striata in the Subtropical Island of Gran Canaria (Canary Islands, Spain)

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1326
Author(s):  
Zivan Gojkovic ◽  
Flavio Guidi ◽  
Begoña Bustamante ◽  
Marianna Venuleo ◽  
Patrícia Alexandra Clemente Janeiro de Assunçao ◽  
...  
Keyword(s):  
Large Scale ◽  
Photosynthetic Activity ◽  
Continuous Cultivation ◽  
Scaling Up ◽  
Good Source ◽  
Gran Canaria ◽  
Tetraselmis Striata ◽  
Large Scale Cultivation ◽  
Photosynthetic Efficiencies

The goal of this study was to determine the feasibility of the large-scale cultivation of locally isolated Tetraselmis striata in different open ponds in Gran Canaria. The biomass productivities were 24.66 ± 0.53 kgDW in 32 days (28.9t/ha/year) for 8000 L indoors, 42.32 ± 0.81 kgDW in 43 days (38.8 t/ha/year) for an 8000 L pond outdoors, and 54.9 ± 0.58 kgDW in 28 days (19.6 t/ha/year) for a 45,000 L pond outdoors. The photosynthetic efficiencies were 1.45 ± 0.03% for an 8000 L pond indoors, 1.95 ± 0.04% for 8000 L outdoors. and 1.10 ± 0.01% for a 45,000 L pond outdoors. The selected strain was fast-growing (µ = 0.21 day−1) and could be rapidly scaled up to 45,000 L; it formed healthy cultures that maintained high photosynthetic activity during long-term cultivation and provided stable biomass productivities, able to grow on urea, which acted as a cheap and effective grazer control. The obtained biomass is a good source of proteins and has an FA profile with a high content of some nutritionally important fatty acids: oleic, α-linolenic (ALA) and EPA. The high ash content in the biomass (>35%) can be reduced by the implementation of additional washing steps after the centrifugation of the culture.

Long term and large-scale cultivation of human hepatoma Hep G2 cells in hollow fiber bioreactor

1991 ◽  
Vol 5 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jiuan J. Liu ◽  
Bor-Shiun Chen ◽  
Te-Feng Tsai ◽  
Yun-Ju Wu ◽  
Victor F. Pang ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Large Scale ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Hollow Fiber Bioreactor ◽  
G2 Cells ◽  
Large Scale Cultivation

scholarly journals Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags

2014 ◽  
Author(s):  
Fanshuang Zhang ◽  
Yuan Yuan ◽  
Jianqiang Wu ◽  
Youhe Gao
Keyword(s):  
Nucleic Acids ◽  
Large Scale ◽  
Room Temperature ◽  
Invasive Disease ◽  
The Body ◽  
Clinical Samples ◽  
Good Source ◽  
Disease Biomarkers ◽  
Non Invasive

Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.

The Necessity for Scale Up in R&D: Approach for Waste Immobilisation in Cement by BNFL

1995 ◽  
Vol 412 ◽  
Author(s):  
Graham A Fairhall ◽  
Eric W Miller
Keyword(s):  
Material Properties ◽  
Large Scale ◽  
Scale Up ◽  
Scaling Up ◽  
Full Scale ◽  
Physical Parameters ◽  
Wide Range ◽  
Regulatory Bodies ◽  
Major Factors

AbstractThe full scale processing of nuclear wastes immobilised in cement utilises a wide range of chemical and physical parameters. The success of this work however, involves many factors and material properties which are affected by the actual scaling up processes. The paper outlines the approach and experience gained by BNFL to recognise and evaluate the major factors involved in order to successfully produce large scale stable products acceptable to the appropriate regulatory bodies and suitable for long term disposal.

scholarly journals Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags

2014 ◽  
Author(s):  
Fanshuang Zhang ◽  
Yuan Yuan ◽  
Jianqiang Wu ◽  
Youhe Gao
Keyword(s):  
Nucleic Acids ◽  
Large Scale ◽  
Room Temperature ◽  
Invasive Disease ◽  
The Body ◽  
Clinical Samples ◽  
Good Source ◽  
Disease Biomarkers ◽  
Non Invasive

Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.

Evolution of Large-Scale Coronal Structures

1994 ◽  
Vol 144 ◽  
pp. 29-33
Author(s):  
P. Ambrož
Keyword(s):  
Magnetic Field ◽  
Field Line ◽  
Large Scale ◽  
Coronal Magnetic Field ◽  
Source Surface ◽  
Solar Cycles ◽  
Characteristic Relationship ◽  
The Magnetic Field ◽  
Coronal Structures

AbstractThe large-scale coronal structures observed during the sporadically visible solar eclipses were compared with the numerically extrapolated field-line structures of coronal magnetic field. A characteristic relationship between the observed structures of coronal plasma and the magnetic field line configurations was determined. The long-term evolution of large scale coronal structures inferred from photospheric magnetic observations in the course of 11- and 22-year solar cycles is described.Some known parameters, such as the source surface radius, or coronal rotation rate are discussed and actually interpreted. A relation between the large-scale photospheric magnetic field evolution and the coronal structure rearrangement is demonstrated.

Computer Analyses in Preventive Health Research

1967 ◽  
Vol 06 (01) ◽  
pp. 8-14 ◽  
Author(s):  
M. F. Collen
Keyword(s):  
Medical Research ◽  
Preventive Medicine ◽  
Health Research ◽  
Large Scale ◽  
Preventive Health ◽  
New Era ◽  
Large Numbers ◽  
Normal Test ◽  
Computer Analyses

The utilization of an automated multitest laboratory as a data acquisition center and of a computer for trie data processing and analysis permits large scale preventive medical research previously not feasible. Normal test values are easily generated for the particular population studied. Long-term epidemiological research on large numbers of persons becomes practical. It is our belief that the advent of automation and computers has introduced a new era of preventive medicine.

(A review) Atlas of especially protected natural areas of Saint Petersburg / Ed. in chief V. N. Khramtsov, T. V. Kovaleva, N. Yu. Natsvaladze. St. Petersburg, 2013. 176 p.

2014 ◽  
pp. 124-129
Author(s):  
Z. V. Karamysheva
Keyword(s):  
Protected Areas ◽  
Large Scale ◽  
Natural Areas ◽  
Natural Protected Areas ◽  
Saint Petersburg ◽  
Natural Processes ◽  
Original Illustrations ◽  
Vegetation Maps ◽  
Protected Natural Areas

The review contains detailed description of the «Atlas of especially protected natural areas of Saint Petersburg» published in 2013. This publication presents the results of long-term studies of 12 natural protected areas made by a large research team in the years from 2002 to 2013 (see References). The Atlas contains a large number of the historical maps, new satellite images, the original illustrations, detailed texts on the nature of protected areas, summary tables of rare species of vascular plants, fungi and vertebrates recorded in these areas. Special attention is paid to the principles of thematic large-scale mapping. The landscape maps, the vegetation maps as well as the maps of natural processes in landscapes are included. Reviewed Atlas deserves the highest praise.

Etude de l'aménagement d'une forêt classée au Mali avec la participation des populations riveraines - Données du problème et éléments de méthodologie | A Study of the Management of a State-Owned Forest in Mali with the Participation of the Population Living nearby - Issue under Investigation and Elements of Methodology

2000 ◽  
Vol 151 (3) ◽  
pp. 80-83
Author(s):  
Pascal Schneider ◽  
Jean-Pierre Sorg
Keyword(s):  
Natural Resources ◽  
Research Methodology ◽  
Large Scale ◽  
Environmental Context ◽  
Forest Resources ◽  
The State ◽  
Commercial Use ◽  
Economic Context ◽  
The People

In and around the state-owned forest of Farako in the region of Sikasso, Mali, a large-scale study focused on finding a compromise allowing the existential and legitimate needs of the population to be met and at the same time conserving the forest resources in the long term. The first step in research was to sketch out the rural socio-economic context and determine the needs for natural resources for autoconsumption and commercial use as well as the demand for non-material forest services. Simultaneously, the environmental context of the forest and the resources available were evaluated by means of inventories with regard to quality and quantity. According to an in-depth comparison between demand and potential, there is a differentiated view of the suitability of the forest to meet the needs of the people living nearby. Propositions for a multipurpose management of the forest were drawn up. This contribution deals with some basic elements of research methodology as well as with results of the study.

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