Evaluation of Wound Healing Potential of Novel Hydrogel Based on Ocimum basilicum and Trifolium pratense Extracts

Plants are an inexhaustible source of compounds with different medicinal properties, suitable as alternative options for the prevention and treatment of various pathologies. They are safe, effective and economical. In this paper, a combined extract made of Ocimum basilicum and Trifolium pratense extracts (EOT) was used for the first time to demonstrate its healing effect on dermal pathologies. To evaluate the wound healing effect of EOT, a novel gel formulation was prepared and subsequently tested in vitro (using the scratch test assay) and in vivo (on an animal model). The in vitro tests demonstrated the complete recovery of the dermal fibroblast monolayer when treated with EOT in a concentration of 50 µg/mL. In vivo results using a hydrogel formulation based on EOT demonstrated improved wound contraction time and complete healing after 13 days of treatment. Moreover, a clinical case of Psoriasis vulgaris was presented, in which one week of treatment led to the significant improvement of the patient’s health. In conclusion, the topical use of the novel gel formulation containing EOT is a successful therapeutic alternative in the treatment of dermal diseases.

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Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽

10.3390/molecules26092488 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2488

Author(s):

Judith Salas-Oropeza ◽

Manuel Jimenez-Estrada ◽

Armando Perez-Torres ◽

Andres Eliu Castell-Rodriguez ◽

Rodolfo Becerril-Millan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Folk Medicine ◽

Fibroblast Cell ◽

In Vitro Tests ◽

Low Concentrations ◽

In Vivo Tests ◽

Wound Healing Activity

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

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Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study

Journal of Functional Biomaterials ◽

10.3390/jfb12040061 ◽

2021 ◽

Vol 12 (4) ◽

pp. 61

Author(s):

Mojtaba Koosha ◽

Hadis Aalipour ◽

Mohammad Javad Sarraf Shirazi ◽

Ali Jebali ◽

Hong Chi ◽

...

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Wound Repair ◽

Dermal Fibroblast ◽

Normal Human Dermal Fibroblast ◽

Gel Content ◽

Freeze Thaw

Chitosan/PVA hydrogel films crosslinked by the freeze–thaw method and containing honey and allantoin were prepared for application as wound dressing materials. The effects of the freeze–thaw process and the addition of honey and allantoin on the swelling, the gel content and the mechanical properties of the samples were evaluated. The physicochemical properties of the samples, with and without the freeze–thaw process, were compared using FTIR, DSC and XRD. The results showed that the freeze–thaw process can increase the crystallinity and thermal stability of chitosan/PVA films. The freeze–thaw process increased the gel content but did not have a significant effect on the tensile strength. The presence of honey reduced the swelling and the tensile strength of the hydrogels due to hydrogen bonding interactions with PVA and chitosan chains. Long-term cell culture experiments using normal human dermal fibroblast (NHDF) cells showed that the hydrogels maintained their biocompatibility, and the cells showed extended morphology on the surface of the hydrogels for more than 30 days. The presence of honey significantly increased the biocompatibility of the hydrogels. The release of allantoin from the hydrogel was studied and, according to the Korsmeyer–Peppas and Weibull models, the mechanism was mainly diffusional. The results for the antimicrobial activity against E. coli and S. aureus bacteria showed that the allantoin-containing samples had a more remarkable antibacterial activity against S. aureus. According to the wound healing experiments, 98% of the wound area treated by the chitosan/PVA/honey hydrogel was closed, compared to 89% for the control. The results of this study suggest that the freeze–thaw process is a non-toxic crosslinking method for the preparation of chitosan/PVA hydrogels with long term biocompatibility that can be applied for wound healing and skin tissue engineering.

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In vitro antimicrobial and in vivo wound healing effect of actinobacterially synthesised nanoparticles of silver, gold and their alloy

RSC Advances ◽

10.1039/c7ra08483h ◽

2017 ◽

Vol 7 (81) ◽

pp. 51729-51743 ◽

Cited By ~ 11

Author(s):

Thangavel Shanmugasundaram ◽

Manikkam Radhakrishnan ◽

Venugopal Gopikrishnan ◽

Krishna Kadirvelu ◽

Ramasamy Balagurunathan

Keyword(s):

Wound Healing ◽

Au Nanoparticles ◽

Healing Effect ◽

Wound Healing Effect ◽

Nanoparticles Of Silver

Antibacterial, antifungal and wound healing potential of actinobacterially synthesised Ag, Au and Ag/Au nanoparticles.

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Increased cutaneous wound healing effect of biodegradable liposomes containing madecassoside: preparation optimization, in vitro dermal permeation, and in vivo bioevaluation

International Journal of Nanomedicine ◽

10.2147/ijn.s105035 ◽

2016 ◽

Vol Volume 11 ◽

pp. 2995-3007 ◽

Cited By ~ 24

Author(s):

Meifeng Liu ◽

Zehao Li ◽

Huijuan Wang ◽

Song Du

Keyword(s):

Wound Healing ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Healing Effect ◽

Wound Healing Effect

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Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing

International Journal of Molecular Sciences ◽

10.3390/ijms20163890 ◽

2019 ◽

Vol 20 (16) ◽

pp. 3890 ◽

Cited By ~ 6

Author(s):

Yu Cheng ◽

Zhang Hu ◽

Yuntao Zhao ◽

Zuhao Zou ◽

Sitong Lu ◽

...

Keyword(s):

Wound Healing ◽

Ftir Spectroscopy ◽

Complete Healing ◽

Carboxymethyl Chitosan ◽

Pathological Examination ◽

In Vitro Tests ◽

Burn Care ◽

Collagen Peptides ◽

Fiber Deposition

Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC–COP) were prepared by covalent coupling and freeze drying. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were then used to characterize the prepared sponges. To evaluate the wound healing activity of the CMC–COP sponges, in vitro tests including cell viability scratch wound healing and scald wound healing experiments were performed in rabbits. Appearance studies revealed the porous nature of sponges and FTIR spectroscopy demonstrated the successful incorporation of COP into CMC. The in vitro scratch assay showed that treatment with CMC–COP sponges (at 100 μg/mL) had significant effects on scratch closure. For burn wounds treated with CMC–COP, regeneration of the epidermis and collagen fiber deposition was observed on day 7, with complete healing of the epidermis and wound on days 14 and 21, respectively. Based on the pathological examination by hematoxylin and eosinstaining, the CMC–COP group demonstrated pronounced wound healing efficiencies. These results confirmed that the CMC–COP treatment enhanced cell migration and promoted skin regeneration, thereby highlighting the potential application of these sponges in burn care.

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Preparation and Evaluation of Atorvastatin-Loaded Nanoemulgel on Wound-Healing Efficacy

Pharmaceutics ◽

10.3390/pharmaceutics11110609 ◽

2019 ◽

Vol 11 (11) ◽

pp. 609 ◽

Cited By ~ 10

Author(s):

Morsy ◽

Abdel-Latif ◽

Nair ◽

Venugopala ◽

Ahmed ◽

...

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Skin Permeation ◽

Healing Effect ◽

Complex Processes ◽

Wound Healing Effect ◽

Histological Architecture ◽

Gel Formulations

Tissue repair and wound healing are complex processes that involve inflammation, granulation, and remodeling of the tissue. The potential of various statins including atorvastatin (ATR) to improve the wound healing effect was established. The aim of this study was to formulate and evaluate the efficacy of topical application of ATR-based nanoemulgel on wound healing. The prepared formulations (ATR gel, ATR emulgel, and ATR nanoemulgel) were evaluated for their physical appearance, rheological behavior, in vitro drug release and ex vivo drug permeation. The in vivo wound healing effect was evaluated in wound-induced rats. The prepared ATR gel formulations showed good physical properties and were comparable. The release profiles of drugs from gel, emulgel, and nanoemulgel were distinct. Skin permeation potential of ATR was significantly (p < 0.05) enhanced when formulated into nanoemulgel. In vivo wound healing studies showed that ATR nanoemulgel exhibited the highest percent wound contraction. Histopathological assessment showed marked improvement in the skin histological architecture after 21 days of ATR nanoemulgel treatment. In conclusion, the data demonstrated here signify the prospective of ATR nanoemulgel as an innovative therapeutic approach in wound healing.

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In vivo incisional wound healing augmented by platelet-derived growth factor and recombinant c-sis gene homodimeric proteins.

Journal of Experimental Medicine ◽

10.1084/jem.167.3.974 ◽

1988 ◽

Vol 167 (3) ◽

pp. 974-987 ◽

Cited By ~ 162

Author(s):

G F Pierce ◽

T A Mustoe ◽

R M Senior ◽

J Reed ◽

G L Griffin ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Tissue Repair ◽

Inflammatory Cells ◽

Cell Types ◽

Platelet Derived Growth Factor ◽

Inflammatory Cell Infiltrate ◽

In Vitro Tests

Human platelet-derived growth factor (hPDGF) is likely to be important in stimulating tissue repair, based upon its in vivo chemotactic and stimulatory activities for inflammatory cells and fibroblasts and upon the presence of PDGF and related proteins in platelets, macrophages, and activated fibroblasts, cell types that make up the milieu of the healing wound. Recombinant human c-sis (rPDGF-B), homodimers of the B chain of PDGF, were compared with hPDGF in vitro. rPDGF-B was immunologically similar to hPDGF and, at identical concentrations, similar to hPDGF in stimulating fibroblast mitogenesis and chemotaxis of polymorphonuclear leukocytes, monocytes, and fibroblasts. Purified hPDGF and rPDGF-B were also tested in vivo for potency in a model of tissue repair using a linear incision wound through rat dermis. A single application of hPDGF or rPDGF-B (2-20 micrograms/wound) in a slow release vehicle at the time of wounding resulted in a dose-dependent, statistically highly significant increase of breaking strength of treated wounds. Wound healing in animals treated with rPDGF-B was 170% stronger and accelerated by 2 d during the first week over control wounds and by 4-6 d over the next 2 wk. Histologic evaluation of growth factor-treated wounds correlated the in vitro chemotactic activity and the accelerated healing of wounds with a striking inflammatory cell infiltrate early after wounding, markedly increased formation of granulation tissue by 4-d, and increased fibrosis by 14 d in comparison to control wounds. The results thus demonstrate that rPDGF-B is fully active in in vitro tests of mitogenesis and chemotaxis and, for the first time, demonstrate directly that PDGF significantly advances wound healing in incisional wounds of experimental animals.

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