Antioxidant Effects of Different Extracts from Root and Aerial Parts of Scorzonera hieraciifolia

The genus Scorzonera has great potential as traditional drugs and foods in several traditional systems including Turkey. In this current work, we investigated antioxidant properties of different extracts from S. hieraciifolia aerial parts and roots. We used different extracts (dichloromethane, ethyl acetate, hexan, methanol and water). To obtain full picture for antioxidant properties, different methods including radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum assay. Total phenolic and flavonoid contents were also calculated for each extract. Antioxidant abilities and total bioactive components depended on the solvents used. The highest level of phenolic was determined in methanol extracts, followed by water, ethyl acetate, hexan and ethyl acetate. Similar to phenolic contents, the best antioxidant properties were obtained by methanol and water extracts. Based on our findings. S. hieraciifolia extracts could be valuable source to combat oxidative stress related diseases such as cancer.

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Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera,V. labrusca, andV. hybrid) Cultivars

BioMed Research International ◽

10.1155/2013/718065 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 26

Author(s):

Shivraj Hariram Nile ◽

S. H. Kim ◽

Eun Young Ko ◽

Se Won Park

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Coumaric Acid ◽

Grape Skin ◽

Phenolic Contents ◽

Grape Skins ◽

Phenolic And Flavonoid

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (−)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400 mg/100 g), followed by Campbell Early and Tamnara (>300 mg/100 g), and then by Red Globe and Ruby Seedless (>250 mg/100 g), and the total phenolic content was the lowest in Italia and Delaware (<60 mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

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ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC AND FLAVONOID CONTENT VARIATIONS OF LEAF EXTRACTS OF LAURUS NOBILIS L. FROM MOROCCO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.29747 ◽

2018 ◽

Vol 11 (12) ◽

pp. 540 ◽

Cited By ~ 3

Author(s):

Amal Taroq ◽

Fatima El Kamari ◽

Imane Aouam ◽

Yassine El Atki ◽

Badiaa Lyoussi ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Flavonoid Content ◽

Laurus Nobilis ◽

Dry Extract ◽

Water Extracts ◽

Phenolic And Flavonoid

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

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The influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense (Schumach & Thonn) seeds

Croatian Journal of Food Science and Technology ◽

10.17508/cjfst.2020.12.2.09 ◽

2020 ◽

Vol 12 (2) ◽

pp. 211-217

Author(s):

Babatunde Oso ◽

Ige Olaoye

Keyword(s):

Aqueous Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Property A ◽

Piper Guineense ◽

Phenolic Contents ◽

Significant Difference ◽

Dpph Scavenging

The study assessed the influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense seeds. Different portions of the aqueous extract of P. guineense seeds were cooked at different temperatures which include 50 oC, 70 oC, and 90 oC for 10 minutes, while the remaining portion was allowed to stand for 10 minutes at room temperature of 29 oC and all were assessed for the evaluations of reducing power, radical scavenging capacities, and total phenolic contents. The results showed that there was a significant increase in the ferric reducing power at 90 oC, however, there was no significant difference between the raw and the cooked P. guineense seeds at 50 oC and 70 oC. In addition, the radical scavenging potential of the extract was highest at 70 oC, however, none of the cooked P. guineense seeds at the selected temperatures exhibited lower DPPH scavenging property. A similar trend was observed for the phenolic content of the extract with a significant reduction at 90 oC. The study suggests that cooking at 70 oC could enhance the antioxidant potentials of P. guineense seeds

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Antioxidant Activity and Isolation of β-Sitosterol from Ethyl Acetate Extract of Aerial Parts of Consolida orientalis

10.20944/preprints201609.0037.v1 ◽

2016 ◽

Author(s):

Abbas Ali Dehpour

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Molecular Formula ◽

Isolation And Identification ◽

Isolation And Purification ◽

Standard Curve ◽

Aerial Parts

General phytochemical screening of the aerial parts of Consolida orientalis revealed the presence of steroids, terpenes, phenolic compounds, saponins, fatty acids, alkaloids. This study was conducted to investigate the bioactivities of extracts, isolation and identification the compounds from aerial parts of C.orientalis. The main goal of the present study is identifying and characterizing the antioxidant activity of the Consolida orientalis and biological isolation of active terpenoid. Aerial parts of the plant were dried at room temperature and reduced to small pieces, followed by using extraction with ethyl acetate percolation. Tree complementary analysis system was used, DPPH free radical scavenging test, total phenolic metabolites and FRAP. The total phenolic content was 38.83±2.09 mg gallic acid corresponding to g-1 extract with regarding to standard curve (y=0.0054x+0.0488, r2=0.995). IC50 value for DPPH radical – scavenging was 987.11±28.66 mgml-1. The extract was exhibited a medium reducing power compared with Vit C. The isolation and purification was afforded white crystalline powder which was subjected to physical, chemical and spectral identification by IR, 1H- and 13C- NMR and GC-MS. Isolated compound was identified as β-sitosterol. That is a terpenoid with melting point 133.4-134.5 ͦ c and with molecular formula C29H50O.

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In vitro Antioxidant Properties of Bersama abyssinica Stem Bark Extracts

Proceedings ◽

10.3390/proceedings2019040021 ◽

2019 ◽

Vol 40 (1) ◽

pp. 21

Author(s):

Kouadio Ibrahime Sinan ◽

Kouadio Bene ◽

Abdurrahman Aktumsek ◽

Gokhan Zengin

Keyword(s):

Ethyl Acetate ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Water Extract ◽

Reducing Power ◽

Stem Bark ◽

Sub Saharan Africa ◽

Total Phenolic ◽

Bersama Abyssinica

Bersama abyssinica, belonging to the Melianthaceae family, is distributed across Sub Saharan Africa. Decoctions of the bark, leaves, and roots of B. abyssinica have been extensively used in traditional medicine to manage many stomach complications such as colic, diarrhea, dysentery, and intestinal worms. In this study, we examined three extracts (ethyl acetate, methanol and water) obtained from B. abyssinica stem barks in terms of antioxidant properties. The antioxidant abilities were investigated by different chemical methods, including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum assay. In addition, total phenolic and flavonoid contents in the extracts were calculated. The highest level of phenolics was determined in water extract (230.83 mg GAE/g extract), followed by methanol (216.79 mg GAE/g extract) and ethyl acetate (100.57 mg GAE/g extract). In same line with total phenolic content, the best antioxidant properties were noted for water and methanol extracts. Our findings suggested that B. abyssinica stem bark extracts could be considered as promising sources of natural antioxidants.

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Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i6.1124-1128.4372 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1124-1128

Author(s):

Fatma Ergün

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Flavonoid ◽

Total Phenolic ◽

Phenolic Substance ◽

Amount Of Substance ◽

Phenolic And Flavonoid

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

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Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

Clinical Phytoscience ◽

10.1186/s40816-019-0123-1 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Praneetha Pallerla ◽

Narsimha Reddy Yellu ◽

Ravi Kumar Bobbala

Keyword(s):

Methanolic Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Liver Homogenate ◽

Reducing Power ◽

Hepatoprotective Activity ◽

Total Phenolic ◽

Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

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Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/612805 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 29

Author(s):

K. Nagendra Prasad ◽

Jing Hao ◽

Chun Yi ◽

Dandan Zhang ◽

Shengxiang Qiu ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Liver Carcinoma ◽

Scavenging Activity ◽

Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem098 ◽

2009 ◽

Vol 6 (2) ◽

pp. 227-231 ◽

Cited By ~ 28

Author(s):

S. A. Adesegun ◽

A. Fajana ◽

C. I. Orabueze ◽

H. A. B. Coker

Keyword(s):

Ascorbic Acid ◽

Leaf Extract ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

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