scholarly journals Developing a Silk Fibroin Composite Film to Scavenge and Probe H2O2 Associated with UV-Excitable Blue Fluorescence

Sensors ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 366
Author(s):  
Tze-Wen Chung ◽  
Chun-Yi Chang ◽  
Chun-Ning Chang ◽  
Chiu-Hsun Liao ◽  
Yun-Jen Jan ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Composite Film ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Blue Fluorescence ◽  
Near Ir ◽  
Good Biocompatibility ◽  
Responsive Element

A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10−3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications.

scholarly journals Fabrication of Silk Fibroin/Graphene Film with High Electrical Conductivity and Humidity Sensitivity

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1774 ◽  
Author(s):  
Haoran Zhang ◽  
Juntao Zhao ◽  
Tieling Xing ◽  
Shenzhou Lu ◽  
Guoqiang Chen
Keyword(s):  
Mechanical Properties ◽  
Electrical Conductivity ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Wearable Sensors ◽  
Graphene Film ◽  
Natural Material ◽  
Casting Method ◽  
Good Biocompatibility ◽  
Minimum Resistance

Silk fibroin (SF) is a natural material with good biocompatibility and excellent mechanical properties, which are complementary to graphene with ultrahigh electrical conductivity. In this study, to maximally combine graphene and silk fibroin, a well-dispersed silk fibroin/graphene suspension was successfully prepared in a simple and effective way. Then we prepared a flexible conductive SF/graphene film with a minimum resistance of 72.1 ± 4.7 Ω/sq by the casting method. It was found that the electrical conductivity of the SF/graphene film was related to the water content of the film, and the variation was more than 200 times. Therefore, it will play an important role in the field of humidity sensors. It also has excellent mechanical properties in both wet and dry states. These unique features make this material a promising future in the fields of biomedical applications, wearable sensors, and implantable internal sensors.

Silver Nanoparticles - Graphene Oxide Nanocomposite for Antibacterial Purpose

2011 ◽  
Vol 364 ◽  
pp. 439-443 ◽  
Author(s):  
Soon Wei Chook ◽  
Chin Hua Chia ◽  
Zakaria Sarani ◽  
Mohd Khan Ayob ◽  
Kah Leong Chee ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Metallic Nanoparticles ◽  
Biomedical Applications ◽  
Food Packaging ◽  
Single Layer ◽  
In Situ Synthesis ◽  
Graphene Oxide Sheets

Graphene oxide (GO) sheets, a single layer of carbon atoms which can be served as substrates for fabricating metallic nanoparticles-GO nanocomposites. In this study, the nanocomposite of silver nanoparticles and graphene oxide were produced via in-situ synthesis and with the addition of chitosan to investigate the formation of silver nanoparticles on the graphene oxide sheets. XRD and UV-Vis studies confirmed the formation of silver nanoparticles on GO sheets, while TEM and FESEM images presented the loading of silver nanoparticles on the GO sheets. The degree of loading and distribution of the silver nanoparticles on the graphene oxide were depend on the method during the formation of silver nanoparticles. The nanocomposites can be potentially used in food packaging and biomedical applications.

scholarly journals Graphene oxide-modified silk fibroin/nanohydroxyapatite scaffold loaded with urine-derived stem cells for immunomodulation and bone regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiachen Sun ◽  
Lang Li ◽  
Fei Xing ◽  
Yun Yang ◽  
Min Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Minimum Cost ◽  
Histological Evaluation ◽  
Micro Computed Tomography ◽  
Cranial Defect ◽  
Good Biocompatibility

Abstract Background The invasive and complicated procedures involving the use of traditional stem cells limit their application in bone tissue engineering. Cell-free, tissue-engineered bones often have complex scaffold structures and are usually engineered using several growth factors (GFs), thus leading to costly and difficult preparations. Urine-derived stem cells (USCs), a type of autologous stem cell isolated noninvasively and with minimum cost, are expected to solve the typical problems of using traditional stem cells to engineer bones. In this study, a graphene oxide (GO)-modified silk fibroin (SF)/nanohydroxyapatite (nHA) scaffold loaded with USCs was developed for immunomodulation and bone regeneration. Methods The SF/nHA scaffolds were prepared via lyophilization and cross-linked with GO using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxy succinimide (NHS). Scaffolds containing various concentrations of GO were characterized using scanning electron microscopy (SEM), the elastic modulus test, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectrometer (XPS). Examinations of cell adhesion, proliferation, viability, morphology, alkaline phosphatase activity, and osteogenesis-related gene expression were performed to compare the osteogenesis-related biological behaviors of USCs cultured on the scaffolds. The effect of USC-laden scaffolds on the differentiation of macrophages was tested using ELISA, qRT-PCR, and immunofluorescence staining. Subcutaneous implantations in rats were performed to evaluate the inflammatory response of the USC-laden scaffolds after implantation. The scaffolds loaded with USCs were implanted into a cranial defect model in rats to repair bone defects. Micro-computed tomography (μCT) analyses and histological evaluation were performed to evaluate the bone repair effects. Results GO modification enhanced the mechanical properties of the scaffolds. Scaffolds containing less than 0.5% GO had good biocompatibility and promoted USC proliferation and osteogenesis. The scaffolds loaded with USCs induced the M2-type differentiation and inhibited the M1-type differentiation of macrophages. The USC-laden scaffolds containing 0.1% GO exhibited the best capacity for promoting the M2-type differentiation of macrophages and accelerating bone regeneration and almost bridged the site of the rat cranial defects at 12 weeks after surgery. Conclusions This composite system has the capacity for immunomodulation and the promotion of bone regeneration and shows promising potential for clinical applications of USC-based, tissue-engineered bones.

Progress in Preparation of Silk Fibroin Microspheres for Biomedical Applications

2020 ◽  
Vol 8 (5) ◽  
pp. 358-371
Author(s):  
Shihe Long ◽  
Yun Xiao ◽  
Xingdong Zhang
Keyword(s):  
Cell Culture ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Major Protein ◽  
High Mechanical Strength ◽  
Precise Control ◽  
Challenges And Opportunities ◽  
Natural Biomaterial ◽  
Good Biocompatibility ◽  
Near Future

: As a natural biomaterial, silk fibroin (SF) holds great potential in biomedical applications with its broad availability, good biocompatibility, high mechanical strength, ease of fabrication, and controlled degradation. With emerging fabrication methods, nanoand microspheres made from SF have brought about unique opportunities in drug delivery, cell culture, and tissue engineering. For these applications, the size and distribution of silk fibroin particles (SFPs) are critical and require precise control during fabrication. Herein, we review common and emerging SFPs fabrication methods and their biomedical applications, and also the challenges and opportunities for SFPs in the near future. : Lay Summary: The application of silk in textile has an extraordinarily long history and new biomedical applications emerged owing to the good biocompatibility and versatile fabrication options of its major protein component, silk fibroin. With the development of nanotechnology and microfabrication, silk fibroin has been fabricated into nano- or microspheres with precisely controlled shape and distribution. In this review, we summarize common and emerging silk fibroin particle fabrication methods and their biomedical applications, and also discuss their challenges and opportunities in the nearest future.

Fabrication and characterization of flexible silk fibroin films reinforced with graphene oxide for biomedical applications

RSC Advances ◽  
2014 ◽  
Vol 4 (76) ◽  
pp. 40312-40320 ◽  
Author(s):  
Lu Wang ◽  
Chunxiang Lu ◽  
Baoping Zhang ◽  
Bin Zhao ◽  
Feng Wu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Fabrication And Characterization

scholarly journals The Fabrication of Cassava Silk Fibroin-Based Composite Film with Graphene Oxide and Chitosan Quaternary Ammonium Salt as a Biodegradable Membrane Material

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Shuqiang Zhao ◽  
Pibo Ma ◽  
Ailan Wan ◽  
Jiwei Huang ◽  
Houyong Yu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Composite Film ◽  
Silk Fibroin ◽  
Ammonium Salt ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Salt ◽  
Breaking Strength ◽  
Composite Films ◽  
The Novel ◽  
Membrane Material

AbstractA novel and excellent composite film was fabricated by simply casting cassava silk fibroin (CSF), chitosan quaternary ammonium salt (HACC), and graphene oxide (GO) in an aqueous solution. Scanning electron microscope images showed that when GO was dispersed in the composite films, the surface of CSF-based composite film became rough, and a wrinkled GO structure could be found. When the content of GO was 0.8%, the film displayed a higher change with respect to the breaking strength and elongation, respectively, up to 97.69 ± 3.69 and 79.11 ± 1.48 MPa, keeping good thermal properties because of the incorporation of GO and HACC. Furthermore, the novel CSF/HACC/GO composite film demonstrates a lower degradation rate, implying the improvement of the resistance to the enzyme solution. Especially in the film with 0.8 wt% GO, the residual mass arrived at 64.35 ± 1.1% of the primary mass after 21 days compared with the CSF/HACC film. This would reclaim the application of silk-based composite films in the biomaterial field.

Ag@AgCl nanoparticles in-situ deposited cellulose acetate/silk fibroin composite film for photocatalytic and antibacterial applications

Cellulose ◽  
2020 ◽  
Vol 27 (13) ◽  
pp. 7721-7737 ◽  
Author(s):  
Xiaohong Wang ◽  
Yuguang Sui ◽  
Jian Jian ◽  
Zhengqiu Yuan ◽  
Jianxian Zeng ◽  
...  
Keyword(s):  
Composite Film ◽  
Cellulose Acetate ◽  
Silk Fibroin ◽  
Agcl Nanoparticles

scholarly journals Conductive In Situ Reduced Graphene Oxide–Silk Fibroin Bionanocomposites

ACS Omega ◽  
2021 ◽  
Author(s):  
Parushuram Nilogal ◽  
Gauthama B. Uppine ◽  
Ranjana Rayaraddi ◽  
Harisha K. Sanjeevappa ◽  
Lavita J. Martis ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Silk Fibroin ◽  
Reduced Graphene

scholarly journals In situ sonochemical reduction and direct functionalization of graphene oxide: A robust approach with thermal and biomedical applications

2017 ◽  
Vol 34 ◽  
pp. 67-77 ◽  
Author(s):  
Shrikant S. Maktedar ◽  
Shantilal S. Mehetre ◽  
Gopal Avashthi ◽  
Man Singh
Keyword(s):  
Graphene Oxide ◽  
Biomedical Applications ◽  
Robust Approach ◽  
Direct Functionalization ◽  
Sonochemical Reduction

Fabrication and biocompatibility of reduced graphene oxide/poly(vinylidene fluoride) composite membranes

RSC Advances ◽  
2015 ◽  
Vol 5 (121) ◽  
pp. 99841-99847 ◽  
Author(s):  
Supeng Pei ◽  
Fei Ai ◽  
Song Qu
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Reduction Method ◽  
Vinylidene Fluoride ◽  
Composite Membranes ◽  
Thermal Reduction ◽  
Reduced Graphene ◽  
Poly Vinylidene Fluoride ◽  
Good Biocompatibility

RGO/PVDF composite membranes with different RGO loadings were prepared by a convenient in situ thermal reduction method and the structural study reveals the good dispersion of RGO in the PVDF, moreover, RGO/PVDF membranes show good biocompatibility.

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