scholarly journals Peptide-Based Capture of Chikungunya Virus E2 Protein Using Porous Silicon Biosensor

Sensors ◽  
2021 ◽  
Vol 21 (24) ◽  
pp. 8248
Author(s):  
Rabeb Layouni ◽  
Tengfei Cao ◽  
Matthew B. Coppock ◽  
Paul E. Laibinis ◽  
Sharon M. Weiss
Keyword(s):  
Porous Silicon ◽  
Chikungunya Virus ◽  
Elevated Temperatures ◽  
E2 Protein ◽  
Protein Capture ◽  
Sensor Platforms ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Improved Stability ◽  
Peptide Functionalization

The detection of pathogens presents specific challenges in ensuring that biosensors remain operable despite exposure to elevated temperatures or other extreme conditions. The most vulnerable component of a biosensor is typically the bioreceptor. Accordingly, the robustness of peptides as bioreceptors offers improved stability and reliability toward harsh environments compared to monoclonal antibodies that may lose their ability to bind target molecules after such exposures. Here, we demonstrate peptide-based capture of the Chikungunya virus E2 protein in a porous silicon microcavity biosensor at room temperature and after exposure of the peptide-functionalized biosensor to high temperature. Contact angle measurements, attenuated total reflectance—Fourier transform infrared spectra, and optical reflectance measurements confirm peptide functionalization and selective E2 protein capture. This work opens the door for other pathogenic biomarker detection using peptide-based capture agents on porous silicon and other surface-based sensor platforms.

scholarly journals Study of the Properties of the Porous Silicon Synthesized by Ag Assisted Chemical Etching

2016 ◽  
Vol 71 ◽  
pp. 40-48 ◽  
Author(s):  
Madhavi Karanam ◽  
G. Mohan Rao ◽  
Shaik Habibuddin ◽  
R. Padmasuvarna
Keyword(s):  
Porous Silicon ◽  
Etching Time ◽  
Photoluminescence Spectra ◽  
Hydrophobic Properties ◽  
Water Contact ◽  
Optical Absorbance ◽  
Angle Measurements ◽  
Static Water Contact Angle ◽  
Contact Angle Measurements ◽  
Static Water

Porous Silicon (PSi) is synthesized by Ag assisted electroless etching and characterized by Scanning electron microscopy (SEM). The effect of etching time on the optical reflectivity, optical absorbance of PSi is investigated. Reflectivity measurements showed that 45% reflectivity Si wafers drops from 45% to 10% for 2 hours etching and 6% for 3 hours etching. The decrease in the reflectivity shows that the PSi can be employed as an anti reflecting substrate in optoelectronic devices. The absorbance measurements reveal that the average absorbance of PSi is 0.60 in the wavelength range 300-800 nm after 2 hours etching. From the photoluminescence spectra it was found that PL intensity of PSi is high compared to bare silicon wafer. Static water contact angle measurements were performed to examine the hydrophobic properties of the PSi prepared under different conditions.

scholarly journals Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 231 ◽  
Author(s):  
Anusuya Pal ◽  
Amalesh Gope ◽  
Germano Iannacchione
Keyword(s):  
Contact Angle ◽  
Elevated Temperatures ◽  
Behavior Changes ◽  
Ambient Conditions ◽  
Angle Measurements ◽  
Colloid System ◽  
Crack Morphology ◽  
Contact Angle Measurements ◽  
Show Evidence ◽  
Insufficient Time

The drying of bio-colloidal droplets can be used in many medical and forensic applications. The whole human blood is the most complex bio-colloid system, whereas bovine serum albumin (BSA) is the simplest. This paper focuses on the drying characteristics and the final morphology of these two bio-colloids. The experiments were conducted by varying their initial concentrations, and the solutions were dried under various controlled substrate temperatures using optical and scanning electron microscopy. The droplet parameters (the contact angle, the fluid front, and the first-order image statistics) reveal the drying process’s unique features. Interestingly, both BSA and blood drying droplets’ contact angle measurements show evidence of a concentration-driven transition as the behavior changes from non-monotonic to monotonic decrease. This result indicates that this transition behavior is not limited to multi-component bio-colloid (blood) only, but may be a phenomenon of a bio-colloidal solution containing a large number of interacting components. The high dilution of blood behaves like the BSA solution. The ring-like deposition, the crack morphology, and the microstructures suggest that the components have enough time to segregate and deposit onto the substrate under ambient conditions. However, there is insufficient time for evaporative-driven segregation to occur at elevated temperatures, as expected.

Nanostructured Polymer Brushes With Reversibly Changing Properties

2002 ◽  
Vol 727 ◽  
Author(s):  
Denys Usov ◽  
Manfred Stamm ◽  
Sergiy Minko ◽  
Christian Froeck ◽  
Andreas Scholl ◽  
...  
Keyword(s):  
Polymer Brushes ◽  
Phase Segregation ◽  
Water Contact ◽  
Angle Measurements ◽  
Nanostructured Polymer ◽  
Contact Angle Measurements ◽  
Vertical Segregation ◽  
Oxygen Plasma Etching ◽  
Grafting From ◽  
Photoemission Electron

AbstractWe investigated the interplay between different mechanisms of the lateral and vertical segregation in the synthesized via “grafting from” approach symmetric A/B (where A and B are poly(styrene-co-2,3,4,5,6-pentafluorostyrene) and poly(methylmethacrylate), respectively) polymer brushes upon exposure to different solvents. We used X-ray photoemission electron spectroscopy and microscopy (X-PEEM), AFM, water contact angle measurements, and oxygen plasma etching to study morphology of the brushes. The ripple morphology after toluene (nonselective solvent) revealed elongated lamellar-like domains of A and B polymers alternating across the surface. The dimple-A morphology consisting of round clusters of the polymer A was observed after acetone (selective solvent for B). The top layer was enriched with the polymer B showing that the brush underwent both the lateral and vertical phase segregation. A qualitative agreement with predictions of SCF theory was found.

Wrinkling Poly(trimethylene 2,5-Furanoate) Free-standing Films: Nanostructure Formation and Physical Properties

2020 ◽  
Author(s):  
Michelina Soccio ◽  
Nadia Lotti ◽  
Andrea Munari ◽  
Esther Rebollar ◽  
Daniel E Martínez-Tong
Keyword(s):  
Irradiation Time ◽  
Polymer Surface ◽  
Laser Source ◽  
Free Standing ◽  
Force Microscopy ◽  
Nanostructure Formation ◽  
Physicochemical Changes ◽  
Angle Measurements ◽  
Atomic Force ◽  
Contact Angle Measurements

<p>Nanostructured wrinkles were developed on fully bio-based poly(trimethylene furanoate) (PTF) films by using the technique of Laser Induced Periodic Surface Structures (LIPSS). We investigated the effect of irradiation time on wrinkle formation using an UV pulsed laser source, at a fluence of 8 mJ/cm2. It was found that the pulse range between 600 and 4800 pulses allowed formation of periodic nanometric ripples. The nanostructured surface was studied using a combined macro- and nanoscale approach. We evaluated possible physicochemical changes taking place on the polymer surface after irradiation by infrared spectroscopy, contact angle measurements and atomic force microscopy. The macroscopic physicochemical properties of PTF showed almost no changes after nanostructure formation, differently from the results previously found for the terephthalic counterparts, as poly(ethyleneterephthalate), PET, and poly(trimethyleneterephthalate), PTT. The surface mechanical properties of the nanostructured PTF were found to be improved, as evidenced by nanomechanical force spectroscopy measurements. In particular, an increased Young’s modulus and higher stiffness for the nanostructured sample were measured. <br></p>

scholarly journals Adhesion Studies during Generative Hybridization of Textile-Reinforced Thermoplastic Composites via Additive Manufacturing

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3888
Author(s):  
Johanna Maier ◽  
Christian Vogel ◽  
Tobias Lebelt ◽  
Vinzenz Geske ◽  
Thomas Behnisch ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Polyamide 6 ◽  
Thermoplastic Composites ◽  
Efficient Production ◽  
Angle Measurements ◽  
Small Series ◽  
Contact Angle Measurements ◽  
Static Tensile ◽  
Pre Treatment ◽  
Manufacturing Technologies

Generative hybridization enables the efficient production of lightweight structures by combining classic manufacturing processes with additive manufacturing technologies. This type of functionalization process allows components with high geometric complexity and high mechanical properties to be produced efficiently in small series without the need for additional molds. In this study, hybrid specimens were generated by additively depositing PA6 (polyamide 6) via fused layer modeling (FLM) onto continuous woven fiber GF/PA6 (glass fiber/polyamide 6) flat preforms. Specifically, the effects of surface pre-treatment and process-induced surface interactions were investigated using optical microscopy for contact angle measurements as well as laser profilometry and thermal analytics. The bonding characteristic at the interface was evaluated via quasi-static tensile pull-off tests. Results indicate that both the bond strength and corresponding failure type vary with pre-treatment settings and process parameters during generative hybridization. It is shown that both the base substrate temperature and the FLM nozzle distance have a significant influence on the adhesive tensile strength. In particular, it can be seen that surface activation by plasma can significantly improve the specific adhesion in generative hybridization.

Carbon-stabilized porous silicon as novel voltammetric sensor platforms

2021 ◽  
pp. 138077
Author(s):  
Clara Pérez-Ràfols ◽  
Keying Guo ◽  
Maria Alba ◽  
Rou Jun Toh ◽  
Núria Serrano ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Voltammetric Sensor ◽  
Sensor Platforms

scholarly journals Epoxy/alumina composite coating on welded steel 316L with excellent wear and anticorrosion properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maryama Hammi ◽  
Younes Ziat ◽  
Zakaryaa Zarhri ◽  
Charaf Laghlimi ◽  
Abdelaziz Moutcine
Keyword(s):  
Electrochemical Behaviour ◽  
Sem Analysis ◽  
Water Contact ◽  
Welded Steel ◽  
Melted Zone ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Alumina Composite ◽  
The Cost ◽  
Good Contrast

AbstractThe main purpose of this study is to elaborate anticorrosive coatings for the welded steel 316L, since this later is widely used in industrial field. Hence, within this work we have studied the electrochemical behaviour of different zones of the welded steel 316 in 1 M HCl media. The macrography study of the welded steel has revealed the different areas with a good contrast. We have stated three different zones, namely; melted zone (MZ), heat affected zone (HAZ) and base metal zone (BM). Impedance studies on welded steel 316L were conducted in 1 M HCl solution, coating of Epoxy/Alumina composite was applied on different zones, in order to reveal the anti-corrosion efficiency in each zone. Scanning electron microscopy (SEM) analysis was undertaken in order to check how far the used coating in such aggressive media protects the studied zones and these findings were assessed by water contact angle measurements. The choice of this coating is based on the cost and the safety. We concluded that the Epoxy/Alumina composite has a good protecting effect regarding welded steel in aggressive media.

Wettability Determination by Equilibrium Contact Angle Measurements: Reservoir Rock- Connate Water System With Injection of CO2

2012 ◽  
Author(s):  
Narjes Shojaikaveh ◽  
Cas Berentsen ◽  
Susanne Eva Johanne Rudolph-Floter ◽  
Karl Heinz Wolf ◽  
William Richard Rossen
Keyword(s):  
Contact Angle ◽  
Water System ◽  
Reservoir Rock ◽  
Equilibrium Contact Angle ◽  
Connate Water ◽  
Angle Measurements ◽  
Contact Angle Measurements

Surface Wettability Enhances Osteoblast Adhesion on Silicon-Substituted Hydroxyapatite Thin Films

2007 ◽  
Vol 330-332 ◽  
pp. 877-880 ◽  
Author(s):  
E.S. Thian ◽  
J. Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield
Keyword(s):  
Thin Films ◽  
Contact Angle ◽  
Actin Filaments ◽  
Culture Study ◽  
Cell Culture Study ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Osteoblast Adhesion ◽  
Lower Contact

Crystalline hydroxyapatite (HA) and 0.8 wt.% silicon-substituted HA (SiHA) thin films were produced using magnetron co-sputtering. These films were subjected to contact angle measurements and in vitro cell culture study using human osteoblast-like (HOB) cells. A wettability study showed that SiHA has a lower contact angle, and thus is more hydrophilic in nature, as compared to HA. Consequently, enhanced cell growth was observed on SiHA at all time-points. Furthermore, distinct and well-developed actin filaments could be seen within HOB cells on SiHA. Thus, this work demonstrated that the surface properties of the coating may be modified by the substitution of Si into the HA structure.

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