scholarly journals Effect of the C/N Ratio on Biodegradation of Ciprofloxacin and Denitrification from Low C/N Wastewater as Assessed by a Novel 3D-BER System

2020 ◽  
Vol 12 (18) ◽  
pp. 7611
Author(s):  
Mahdi Hassan ◽  
Guangcan Zhu ◽  
Zhonglian Yang ◽  
Yongze Lu ◽  
Yan Lang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Denitrifying Bacteria ◽  
Vital Role ◽  
Current Intensity ◽  
The Novel ◽  
The Past ◽  
Class Level ◽  
The Ideal

Emerging pollutants in the form of pharmaceuticals have drawn international attention during the past few decades. Ciprofloxacin (CIP) is a common drug widely found in effluents from hospitals, industrial and different wastewater treatment plants, as well as rivers. In this work, the lab-scale 3D-BER system was established, and more than 90% of the antibiotic CIP was removed from Low C/N wastewater. The best results were obtained with the current intensity being taken into account, and a different C/N ratio significantly improved the removal of CIP and nitrates when the ideal conditions were C/N = 1.5–3.5, pH = 7.0–7.5 and I = 60 mA. The highest removal efficiency occurred when CIP = 94.2%, NO3−-N = 95.5% and total nitrogen (TN) = 84.3%, respectively. In this novel system, the autotrophic-heterotrophic denitrifying bacteria played a vital role in the removal of CIP and an enhanced denitrification process. Thus, autotrophic denitrifying bacteria uses CO2 and H2 as carbon sources to reduce nitrates to N2. This system has the assortment and prosperous community revealed at the current intensity of 60 mA, and the analysis of bacterial community structure in effluent samples fluctuates under different conditions of C/N ratios. Based on the results of LC-MS/MS analysis, the intermediate products were proposed after efficient biodegradation of CIP. The microbial community on biodegrading was mostly found at phylum, and the class level was dominantly responsible for the NO3−-N and biodegradation of CIP. This work can provide some new insights towards the biodegradation of CIP and the efficient removal of nitrates from low C/N wastewater treatment through the novel 3D-BER system.

scholarly journals Effect of C/N Ratio on Biodegradation of Ciprofloxacin and Denitrification from Low C/N Wastewater by a Novel 3D-BER System

2020 ◽  
Author(s):  
Mahdi Hassan ◽  
Guangcan Zhu ◽  
Zhonglian Yang ◽  
Yongze Lu ◽  
Yan Lang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Denitrifying Bacteria ◽  
Vital Role ◽  
Current Intensity ◽  
The Novel ◽  
Efficient Removal ◽  
Class Level ◽  
The Ideal

Emerging pollutants as pharmaceuticals have been focusing international attention for few decades. Ciprofloxacin (CIP) is a common drug widely found in effluents from hospitals, industrial and different wastewater treatment plants, as well as rivers. In this work, the lab-scale 3D-BER system was established, and more than 90% of the antibiotic CIP removal from the Low C/N wastewater. Best results were obtained with current intensity, and different C/N ratio significantly improve the removal of CIP and nitrates, when the ideal conditions were; C/N = 1.5-3.5, pH =7.0-7.5, and I = 60 mA. The highest removal efficiency of CIP = 94.20 %, NO3--N= 95.53 % and total nitrogen (TN) = 84.27 %, respectively. In this novel system, the autotrophic-heterotrophic denitrifying bacteria played vital role for the removal of CIP and enhanced denitrification process. Thus, autotrophic denitrifying bacteria uses CO2 and H2 as carbon sources to reduce nitrates to N2. This system has the assortment and prosperous community revealed at the current intensity of 60 mA, and the analysis of bacterial community structure in effluent samples fluctuates under different condition of C/N ratios. According to the results of LC-MS/MS analysis, the intermediate products were proposed after efficient biodegradation of CIP. Microbial community on biodegrading was mostly found at phylum, and class level was dominantly responsible for the NO3--N and biodegradation of CIP. This work can provide some new insights towards the biodegradation of CIP and the efficient removal of nitrates from low C/N wastewater treatment by the novel 3D-BER system.

scholarly journals In Situ Characterization ofNitrospira-Like Nitrite-Oxidizing Bacteria Active in Wastewater Treatment Plants

2001 ◽  
Vol 67 (11) ◽  
pp. 5273-5284 ◽  
Author(s):  
Holger Daims ◽  
Jeppe L. Nielsen ◽  
Per H. Nielsen ◽  
Karl-Heinz Schleifer ◽  
Michael Wagner
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
Laser Scanning ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Rrna Gene ◽  
Phylogenetic Affiliation

ABSTRACT Uncultivated Nitrospira-like bacteria in different biofilm and activated-sludge samples were investigated by cultivation-independent molecular approaches. Initially, the phylogenetic affiliation of Nitrospira-like bacteria in a nitrifying biofilm was determined by 16S rRNA gene sequence analysis. Subsequently, a phylogenetic consensus tree of theNitrospira phylum including all publicly available sequences was constructed. This analysis revealed that the genusNitrospira consists of at least four distinct sublineages. Based on these data, two 16S rRNA-directed oligonucleotide probes specific for the phylum and genus Nitrospira, respectively, were developed and evaluated for suitability for fluorescence in situ hybridization (FISH). The probes were used to investigate the in situ architecture of cell aggregates ofNitrospira-like nitrite oxidizers in wastewater treatment plants by FISH, confocal laser scanning microscopy, and computer-aided three-dimensional visualization. Cavities and a network of cell-free channels inside the Nitrospiramicrocolonies were detected that were water permeable, as demonstrated by fluorescein staining. The uptake of different carbon sources byNitrospira-like bacteria within their natural habitat under different incubation conditions was studied by combined FISH and microautoradiography. Under aerobic conditions, theNitrospira-like bacteria in bioreactor samples took up inorganic carbon (as HCO3 − or as CO2) and pyruvate but not acetate, butyrate, and propionate, suggesting that these bacteria can grow mixotrophically in the presence of pyruvate. In contrast, no uptake by theNitrospira-like bacteria of any of the carbon sources tested was observed under anoxic or anaerobic conditions.

scholarly journals Hydrogen via reforming aqueous ammonia and biomethane co-products of wastewater treatment: environmental and economic sustainability

2020 ◽  
Vol 4 (11) ◽  
pp. 5835-5850
Author(s):  
Oliver Grasham ◽  
Valerie Dupont ◽  
Timothy Cockerill ◽  
Miller Alonso Camargo-Valero ◽  
Martyn V. Twigg
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Aqueous Ammonia ◽  
Economic Sustainability ◽  
The Novel ◽  
Novel Method

Wastewater treatment plants can be centres of green H2 production via the novel method presented for co-reforming of bio-ammonia and bio-methane.

scholarly journals Research on Forward Osmosis Membrane Technology Still Needs Improvement in Water Recovery and Wastewater Treatment

Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 107 ◽  
Author(s):  
Li Li ◽  
Wenxin Shi ◽  
Shuili Yu
Keyword(s):  
Wastewater Treatment ◽  
Forward Osmosis ◽  
Solute Flux ◽  
Successful Implementation ◽  
Water Recovery ◽  
The Novel ◽  
Permeate Flux ◽  
Membrane Material ◽  
The Past ◽  
Research Hotspots

Forward osmosis (FO) has become an evolving membrane separation technology to recover water due to its strong retention capacity, sustainable membrane fouling, etc. Although a good deal of research has been extensively investigated in the past decades, major challenges still remain as follows: (1) the novel FO membrane material properties, which significantly influence the fouling of the FO membranes, the intolerance reverse solute flux (RSF), the high concentration polarization (CP), and the low permeate flux; (2) novel draw solution preparation and utilization; (3) salinity build-up in the FO system; (4) the successful implementation of the FO process. This work critically reviews the last five years’ literature in development of the novel FO membrane material, structure in modification, and preparation, including comparison and analysis on the traditional and novel draw solutes coupled with their effects on FO performance; application in wastewater treatment, especially hybrid system and integrated FO system; fouling mechanism; and cleaning strategy as discussed in the literature. The current barriers of the research results in each hotspot and the areas that can be improved are also analyzed in detail. The research hotspots in the research and development of the novel membrane materials in various countries and regions have been compared in recent years, and the work of variation in pop research hotspots in the past 10 years has been analyzed and the ideas that fill the blank gaps also have been proposed.

Substrate uptake by Gordonia amarae in activated sludge foams by FISH-MAR

2006 ◽  
Vol 54 (1) ◽  
pp. 39-45 ◽  
Author(s):  
E.L. Carr ◽  
K.L. Eales ◽  
R.J. Seviour
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Mycolic Acid ◽  
Substrate Uptake ◽  
Wastewater Treatment Process ◽  
Pure Cultures ◽  
Gordonia Amarae

Gordonia amarae is a right-angled branching filament belonging to the mycolic acid-containing Actinobacteria which is commonly found in many foaming activated sludge wastewater treatment plants. Although studies on different substrates as sole carbon sources by pure cultures of G. amarae have been carried out, none have examined substrate uptake by this organism in situ. Uptake of several hydrophilic and hydrophobic substrates by G. amarae was evaluated in situ using a combination of fluorescence in situ hybridization and microautoradiography. G. amarae could assimilate a range of both hydrophilic and hydrophobic substrates. From the data, G. amarae appears to be physiologically active under aerobic, anaerobic and anoxic condition (NO2 and NO3) for some substrates. This might explain why attempts to control foaming caused by G. amarae using anoxic and anaerobic selectors have been unsuccessful. This study emphasizes that bacteria can behave differently in situ to pure cultures and that it is important to evaluate the in situ physiology of these bacteria if we are to better understand their role in the wastewater treatment process.

Community dynamics of denitrifying bacteria in full-scale wastewater treatment plants

2016 ◽  
Vol 37 (18) ◽  
pp. 2358-2367 ◽  
Author(s):  
Magdalena Zielińska ◽  
Paulina Rusanowska ◽  
Joanna Jarząbek ◽  
Jeppe Lund Nielsen
Keyword(s):  
Wastewater Treatment ◽  
Community Dynamics ◽  
Wastewater Treatment Plants ◽  
Denitrifying Bacteria ◽  
Full Scale

Optimizing Polymer Consumption in Sludge Dewatering Applications

1990 ◽  
Vol 22 (7-8) ◽  
pp. 261-267
Author(s):  
P. M. Crawford
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Operating Performance ◽  
Water Systems ◽  
Full Scale ◽  
Sludge Dewatering ◽  
The Past ◽  
Sludge Conditioning ◽  
Original Objective ◽  
Device Operation

As we move into the 1990's, upgrading of existing wastewater treatment plants is becoming a very important consideration. Although upgrading can take many forms, one of the most economic is to optimize the performance of the existing equipment and structures. In the realm of sludge dewatering, also an increasingly important topic, an area which has received little attention in the past is the control of the sludge conditioning process prior to dewatering. In conjunction with the Wastewater Technology Centre in Burlington, Canada, ZENON Water Systems Inc. has developed the Sludge Conditioning Controller (SCC) to fulfill this need in the wastewater marketplace. A description of both the hardware and software aspects of the SCC are presented. In addition, typical operating performance of the microprocessor-based system is shown. Experience with full scale systems has revealed that the benefits associated with the SCC far exceed the original objective of saving polymer. The others include automation of the dewatering device operation, increased capacity, and more uniform performance of the dewatering machine.

scholarly journals Casimicrobium huifangae gen. nov., sp. nov., a Ubiquitous “Most-Wanted” Core Bacterial Taxon from Municipal Wastewater Treatment Plants

2019 ◽  
Vol 86 (4) ◽  
Author(s):  
Yang Song ◽  
Cheng-Ying Jiang ◽  
Zong-Lin Liang ◽  
Bao-Jun Wang ◽  
Yong Jiang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Short Chain Fatty Acids ◽  
Municipal Wastewater Treatment ◽  
Content Type ◽  
Bacterial Taxon ◽  
Municipal Wastewater Treatment Plants

ABSTRACT Microorganisms in wastewater treatment plants (WWTPs) play a key role in the removal of pollutants from municipal and industrial wastewaters. A recent study estimated that activated sludge from global municipal WWTPs harbors 1 × 109 to 2 × 109 microbial species, the majority of which have not yet been cultivated, and 28 core taxa were identified as “most-wanted” ones (L. Wu, D. Ning, B. Zhang, Y. Li, et al., Nat Microbiol 4:1183–1195, 2019, https://doi.org/10.1038/s41564-019-0426-5). Cultivation and characterization of the “most-wanted” core bacteria are critical to understand their genetic, physiological, phylogenetic, and ecological traits, as well as to improve the performance of WWTPs. In this study, we isolated a bacterial strain, designated SJ-1, that represents a novel cluster within Betaproteobacteria and corresponds to OTU_16 within the 28 core taxa in the “most-wanted” list. Strain SJ-1 was identified and nominated as Casimicrobium huifangae gen. nov., sp. nov., of a novel family, Casimicrobiaceae. C. huifangae is ubiquitously distributed and is metabolically versatile. In addition to mineralizing various carbon sources (including carbohydrates, aromatic compounds, and short-chain fatty acids), C. huifangae is capable of nitrate reduction and phosphorus accumulation. The population of C. huifangae accounted for more than 1% of the bacterial population of the activated sludge microbiome from the Qinghe WWTP, which showed seasonal dynamic changes. Cooccurrence analysis suggested that C. huifangae was an important module hub in the bacterial network of Qinghe WWTP. IMPORTANCE The activated sludge process is the most widely applied biotechnology and is one of the best ecosystems to address microbial ecological principles. Yet, the cultivation of core bacteria and the exploration of their physiology and ecology are limited. In this study, the core and novel bacterial taxon C. huifangae was cultivated and characterized. This study revealed that C. huifangae functioned as an important module hub in the activated sludge microbiome, and it potentially plays an important role in municipal wastewater treatment plants.

Biological nitrification and denitrification of opto-electronic industrial wastewater

2003 ◽  
Vol 48 (8) ◽  
pp. 27-34 ◽  
Author(s):  
T.-K. Chen ◽  
C.-H. Ni ◽  
J.-N. Chen
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Organic Nitrogen ◽  
Wastewater Treatment Plants ◽  
Biological Nutrient Removal ◽  
Nitrogen And Phosphorus ◽  
Mixed Liquor ◽  
The Past ◽  
Two Factors ◽  
Recycle Rate

Development and application of biological nutrient removal processes accelerated significantly over the past decade due to more stringent nutrients (nitrogen and phosphorus) discharge limits being imposed on wastewater treatment plants. The opto-electronic industry has developed very fast over the past decade in the world. The wastewater often contains a significant quantity of organic nitrogen compounds and has a ratio of over 95% in organic nitrogen (Org-N) to total nitrogen (T-N). In this study, a 2-stage Anoxic/Aerobic pre-denitrification process was established and the efficiency of wastewater treatment was evaluated. Wastewater from an actual LCD-plant was obtained as the sample for looking into the feasibility of opto-electronic industrial wastewater treatment. Hydraulic retention time (HRT) and mixed liquor recycle rate (MLR) were controlled independently to distinguish between the effects of these two factors. Under suitable HRT and mixed liquor recycle ratio, effluents of NH4-N, NOx-N and COD can fall below 20 mg/l, 30 mg/l and 80 mg/l.

Distillery wastes as external carbon sources for denitrification in municipal wastewater treatment plants

2012 ◽  
Vol 65 (9) ◽  
pp. 1583-1590 ◽  
Author(s):  
K. Czerwionka ◽  
J. Makinia ◽  
M. Kaszubowska ◽  
J. Majtacz ◽  
M. Angowski
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Biological Nutrient Removal ◽  
Yield Coefficient ◽  
Municipal Wastewater Treatment ◽  
Reject Water ◽  
Fusel Oils ◽  
By Products

In this study, by-products from alcohol production were examined in terms of their potential application as external carbon sources for enhancing denitrification in biological nutrient removal systems. Three types of batch tests were used to compare the effects of the distillery by-products, such as fusel oil, syrup and reject water, on the non-acclimated activated sludge. Much higher nitrate utilization rates (NURs) were observed for the latter two carbon sources. In the conventional NUR measurements (one-phase experiments), the observed NURs with syrup and reject water were 3.2–3.3 g N/(kg VSS h) compared with 1.0 g N/(kg VSS h) obtained for fusel oils from two different distilleries. When the carbon sources were added at the beginning of the anoxic phase preceded by an anaerobic phase (two-phase experiments), the NURs were 4.2 g N/(kg VSS h) (syrup and reject water) and 2.4–2.7 g N/(kg VSS h) (fusel oils). The heterotrophic yield coefficient, determined based on the conventional OUR measurements, varied in a relatively narrow range (0.72–0.79 g COD/g COD) for all the examined carbon sources. Due to advantageous composition (much higher COD concentrations and COD/N ratios), fusel is a preferred carbon source for practical handling in full-scale wastewater treatment plants.

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