scholarly journals Variation of the Main Alkaloid Content in Equisetum palustre L. in the Light of Its Ontogeny

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 710
Author(s):  
Jürgen Müller ◽  
Philipp Mario Puttich ◽  
Till Beuerle
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Developmental Stages ◽  
Alkaloid Content ◽  
Ontogenetic Stage ◽  
Poisonous Plants ◽  
Major Health ◽  
Growing Seasons ◽  
Wet Grasslands ◽  
Two Populations

Marsh horsetail (Equisetum palustre L.) is one of the most poisonous plants of wet grasslands in the northern hemisphere, which poses a major health threat to livestock. Available data on the levels of its main alkaloids are currently contradictory due to the inadequate analytical methods and the wide variation in toxicity levels reported. Here, we tested the hypothesis that the ontogenetic stage of plant development may explain a significant part of the variations in the main Equisetum-type alkaloids. Two populations of marsh horsetail were sampled over two growing seasons. The plant material was classified according to their developmental stages and subsequently the main alkaloids were determined by hydrophilic interaction liquid chromatography and high-performance liquid chromatography electrospray tandem mass spectrometry (HILIC HPLC-ESI-MS/MS) analysis. ANOVA revealed significant effects of the ontogenetic stage but not the site on the main Equisetum-type alkaloids (sum of palustrine and palustridiene) ranging from 213 to 994 mg/kg dry matter (DM). The highest alkaloid content was found in the stages of early development. Not the season itself, but the growth temperature co-influenced the alkaloid content. Our results help to resolve the seemingly contradictory information provided by previous studies on the toxicity of E. palustre and are of practical relevance for the prevention of contamination risks in wet grassland use.

Newborn screening for haemoglobinopathies by high performance liquid chromatography (HPLC): diagnostic utility of different approaches in resource-poor settings

2014 ◽  
Vol 52 (12) ◽  
Author(s):  
Dipti S. Upadhye ◽  
Dipty L. Jain ◽  
Yogesh L. Trivedi ◽  
Anita H. Nadkarni ◽  
Kanjaksha Ghosh ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Newborn Screening ◽  
Sickle Cell ◽  
High Performance ◽  
Diagnostic Utility ◽  
Gene Deletions ◽  
Major Health ◽  
Sickle Cell Disorders ◽  
Normal Baby

AbstractSickle cell disease is a major health burden in India. The aim of the study was to compare the diagnostic utility of two different approaches on automated high performance liquid chromatography (HPLC) for newborn screening for sickle cell disorders and other haemoglobinopathies in India.Newborn babies of sickle heterozygous mothers were tested by HPLC using two different kits, the β-thal short kit, which is routinely used for screening for haemoglobinopathies in most laboratories, and the sickle cell short kit which is specific only for neonatal samples. Confirmation of the sickle and α genotypes was done by molecular analysis.Of the 601 babies tested, 276 were normal, 284 were sickle heterozygous and 41 were sickle homozygous using the β-thal short kit. Using the sickle cell short kit, a discrepancy was seen in one newborn sample where a normal baby was identified as a sickle heterozygous baby. α-Genotyping was done in 42 babies and 16 of them had α gene deletions. The presence of α thalassaemia could be suspected in 15 of these 16 babies based on a spike at the start of the chromatogram using the β-thal short kit. In comparison, using the sickle cell short kit the diagnosis of α thalassaemia was difficult based on the percentage of the FAST peak. Further, other rare α chain Hb variants were also missed.The β-thal short kit was more versatile than the sickle cell short kit for screening for haemoglobinopathies in newborns in our population.

Quantitative Analysis of Flavonoids in the Flowers and Leaves of Ficaria verna Huds.

2003 ◽  
Vol 58 (9-10) ◽  
pp. 763-765 ◽  
Author(s):  
Michał Tomczyk ◽  
Jan Gudej
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
High Performance ◽  
Hplc Analysis ◽  
Flavonoid Content ◽  
Growing Seasons ◽  
Ficaria Verna

AbstractThe quantitative determination of flavonoid compounds in flowers and leaves of Ficaria verna Huds. (Ranunculaceae) was carried out in different growing seasons of the plant, using Christ-Müller’s method (Polish Pharmacopoeia, 1999) and high performance liquid chromatography (HPLC) analysis after acid hydrolysis. The flavonoid content was much higher in flowers than in leaves.

scholarly journals Profile of Immunoglobulin G N-Glycome in COVID-19 Patients: A Case-Control Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Haifeng Hou ◽  
Huan Yang ◽  
Pengcheng Liu ◽  
Changwu Huang ◽  
Meng Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Immune Responses ◽  
High Performance ◽  
Case Control Study ◽  
Case Control ◽  
Hydrophilic Interaction ◽  
Major Health ◽  
Alternative Complement ◽  
Dependent Cell ◽  
Control Study

Coronavirus disease 2019 (COVID-19) remains a major health challenge globally. Previous studies have suggested that changes in the glycosylation of IgG are closely associated with the severity of COVID-19. This study aimed to compare the profiles of IgG N-glycome between COVID-19 patients and healthy controls. A case-control study was conducted, in which 104 COVID-19 patients and 104 age- and sex-matched healthy individuals were recruited. Serum IgG N-glycome composition was analyzed by hydrophilic interaction liquid chromatography with the ultra-high-performance liquid chromatography (HILIC-UPLC) approach. COVID-19 patients have a decreased level of IgG fucosylation, which upregulates antibody-dependent cell cytotoxicity (ADCC) in acute immune responses. In severe cases, a low level of IgG sialylation contributes to the ADCC-regulated enhancement of inflammatory cytokines. The decreases in sialylation and galactosylation play a role in COVID-19 pathogenesis via the activation of the lectin-initiated alternative complement pathway. IgG N-glycosylation underlines the complex clinical phenotypes of SARS-CoV-2 infection.

scholarly journals N-glycan profiling of bovine follicular fluid at key dominant follicle developmental stages

Reproduction ◽  
2014 ◽  
Vol 148 (6) ◽  
pp. 569-580 ◽  
Author(s):  
T Tharmalingam-Jaikaran ◽  
S W Walsh ◽  
P A McGettigan ◽  
O Potter ◽  
W B Struwe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Follicular Fluid ◽  
High Performance ◽  
Developmental Stages ◽  
Exchange Chromatography ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Glycan Profile ◽  
Dairy Heifers

Follicular fluid (FF), an important microenvironment for the development of oocytes, contains many proteins that are glycosylated withN-linked glycans. This study aimed i) to present an initial analysis of theN-linked glycan profile of bovine FF using hydrophilic interaction liquid chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC)-based separations and subsequent liquid chromatography–mass spectrometry/mass spectrometry analysis; ii) to determine differences in theN-glycan profile between FF from dominant and subordinate follicles from dairy heifers and lactating dairy cows and iii) to identify alterations in theN-glycan profile of FF during preovulatory follicle development using newly selected, differentiated (preovulatory) and luteinised dominant follicles from dairy heifers and lactating cows. We found that the majority of glycans on bovine FF are based on biantennary hypersialylated structures, where the glycans are sialylated on both the galactose andN-acetylglucosamine terminal sugars. A comparison of FFN-glycans from cows and heifers indicated higher levels of nonsialylated glycans with a lower proportion of sialylated glycans in cows than in heifers. Overall, as the follicle develops from Selection, Differentiation and Luteinisation in both cows and heifers, there is an overall decrease in sialylated structures on FFN-glycans.

Sign in / Sign up

Export Citation Format

Share Document