scholarly journals In Vitro Lung Models and Their Application to Study SARS-CoV-2 Pathogenesis and Disease

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 792
Author(s):  
Natalie Heinen ◽  
Mara Klöhn ◽  
Eike Steinmann ◽  
Stephanie Pfaender
Keyword(s):  
Cell Culture ◽  
Ex Vivo ◽  
Treatment Options ◽  
Pharmaceutical Companies ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Novel Treatment ◽  
Cell Culture Systems ◽  
Drug Efficiency

SARS-CoV-2 has spread across the globe with an astonishing velocity and lethality that has put scientist and pharmaceutical companies worldwide on the spot to develop novel treatment options and reliable vaccination for billions of people. To combat its associated disease COVID-19 and potentially newly emerging coronaviruses, numerous pre-clinical cell culture techniques have progressively been used, which allow the study of SARS-CoV-2 pathogenesis, basic replication mechanisms, and drug efficiency in the most authentic context. Hence, this review was designed to summarize and discuss currently used in vitro and ex vivo cell culture systems and will illustrate how these systems will help us to face the challenges imposed by the current SARS-CoV-2 pandemic.

scholarly journals Vessel-on-a-chip models for studying microvascular physiology, transport, and function in vitro

2020 ◽  
Author(s):  
Savannah R. Moses ◽  
Jonathan J. Adorno ◽  
Andre F. Palmer ◽  
Jonathan W. Song
Keyword(s):  
Cell Culture ◽  
Tissue Level ◽  
Culture Conditions ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Microscale Flow ◽  
Flow Phenomena ◽  
Defined Culture ◽  
And Function

To understand how the microvasculature grows and remodels, researchers require reproducible systems that emulate the function of living tissue. Innovative contributions toward fulfilling this important need have been made by engineered microvessels assembled in vitro using microfabrication techniques. Microfabricated vessels, commonly referred to as "vessels on a chip," are from a class of cell culture technologies that uniquely integrate microscale flow phenomena, tissue-level biomolecular transport, cell-cell interactions, and proper 3-D extracellular matrix environments under well-defined culture conditions. Here, we discuss the enabling attributes of microfabricated vessels that make these models more physiological compared to established cell culture techniques, and the potential of these models for advancing microvascular research. This review highlights the key features of microvascular transport and physiology, critically discusses the strengths and limitations of different microfabrication strategies for studying the microvasculature, and provides a perspective on current challenges and future opportunities for vessel on a chip models.

Imitating Hypoxia and Tumor Microenvironment with Immune Evasion by Employing Three Dimensional in vitro Cellular Models: Impressive Tool in Drug Discovery

2021 ◽  
Vol 16 ◽  
Author(s):  
Suman Kumar Ray ◽  
Sukhes Mukherjee
Keyword(s):  
Cell Culture ◽  
Tumor Microenvironment ◽  
Immune Evasion ◽  
Cancer Biology ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Hypoxic Tumor

: The heterogeneous tumor microenvironment is exceptionally perplexing and not wholly comprehended. Different multifaceted alignments lead to the generation of oxygen destitute situations within the tumor niche that modulate numerous intrinsic tumor microenvironments. Disentangling these communications is vital for scheming practical therapeutic approaches that can successfully decrease tumor allied chemotherapy resistance by utilizing the innate capability of the immune system. Several research groups have concerned with a protruding role for oxygen metabolism along with hypoxia in the immunity of healthy tissue. Hypoxia in addition to hypoxia-inducible factors (HIFs) in the tumor microenvironment plays an important part in tumor progression and endurance. Although numerous hypoxia-focused therapies have shown promising outcomes both in vitro and in vivo these outcomes have not effectively translated into clinical preliminaries. Distinctive cell culture techniques have utilized as an in vitro model for tumor niche along with tumor microenvironment and proficient in more precisely recreating tumor genomic profiles as well as envisaging therapeutic response. To study the dynamics of tumor immune evasion, three-dimensional (3D) cell cultures are more physiologically important to the hypoxic tumor microenvironment. Recent research has revealed new information and insights into our fundamental understanding of immune systems, as well as novel results that have been established as potential therapeutic targets. There are a lot of patented 3D cell culture techniques which will be highlighted in this review. At present notable 3D cell culture procedures in the hypoxic tumor microenvironment, discourse open doors to accommodate both drug repurposing, advancement, and divulgence of new medications and will deliberate the 3D cell culture methods into standard prescription disclosure especially in the field of cancer biology which will be discussing here.

Cell Culture–Based Tissue Engineering as an Alternative to Bone Grafts in Implant Dentistry: A Literature Review

2012 ◽  
Vol 38 (S1) ◽  
pp. 538-545 ◽  
Author(s):  
Daniel Gonçalves Boeckel ◽  
Rosemary Sadami Arai Shinkai ◽  
Márcio Lima Grossi ◽  
Eduardo Rolim Teixeira
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Literature Review ◽  
Bone Tissue ◽  
Ex Vivo ◽  
Maxillofacial Surgery ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Implant Dentistry ◽  
A New Technique

Several biomaterials and techniques for bone grafting have been described in the literature for atresic bone tissue replacement caused by edentulism, surgical resectioning, and traumas. A new technique involves tissue engineering, a promising option to replace bone tissue and solve problems associated with morbidity of autogenous grafting. This literature review aims to describe tissue-engineering techniques using ex vivo cell culture as an alternative to repair bone maxillary atresias and discuss the concepts and potentials of bone regeneration through cell culture techniques as an option for restorative maxillofacial surgery.

scholarly journals Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1656
Author(s):  
Christoph Grün ◽  
Brigitte Altmann ◽  
Eric Gottwald
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
3D Cell Culture ◽  
Cell Types ◽  
Cell Contact ◽  
Systematic Analysis ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
3D Microenvironment

Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020.

Advanced cell culture techniques for cancer research

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Karolina Balik ◽  
Karolina Matulewicz ◽  
Paulina Modrakowska ◽  
Jolanta Kozłowska ◽  
Xavier Montane ◽  
...  
Keyword(s):  
Cell Culture ◽  
Basic Research ◽  
Animal Testing ◽  
Culture Methods ◽  
Cancer Treatments ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Strong Trend ◽  
Anti Cancer

Abstract The incessant increase number of cancer cases, motivates scientists to constantly develop and search for new therapies. Along with the dynamic development of anti-cancer drugs and therapies, we are witnessing huge progress in the world of science - the development of personalized medicine. An inseparable element is also a very strong trend in the development of new in vitro animal models for chemotherapeutic research. Cell cultures are commonly undertaken by research models before animal testing. They are the basis for the development of new diagnostic and cancer treatments. It should be emphasized that basic research is a strong foundation for any therapy introduced. This chapter provides an overview of the modern cell culture techniques that are currently developing, which allow the introduction of modern models that reflect the organs and physiological system. Currently available cell culture methods are a key aspect of studying these interactions, however, a method that eliminates the limitations of standard methods is still being sought.

scholarly journals A Unique 3D In Vitro Cellular Invasion Assay

2012 ◽  
Vol 17 (8) ◽  
pp. 1088-1095 ◽  
Author(s):  
Daniela F. Quail ◽  
Tamara J. Maciel ◽  
Kem Rogers ◽  
Lynne M. Postovit
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
3D Cell Culture ◽  
Breast Cancer Cell Lines ◽  
Invasion Assay ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Cellular Invasion

Three-dimensional (3D) cell culture techniques using a bioreactor have been used to co-culture various breast cancer cell lines. Comparisons between 3D co-cultures containing different proportions of breast cancer cell lines have been made with respect to cluster size, cell surface marker distribution, and Ki67 expression. Furthermore, an observed difference in invasion through collagen between co-cultures has been briefly reported. However, these assays have not yet been developed into a quantifiable methodology to assess the effects of drugs and/or microenvironments on cellular invasion. From a cancer perspective, two important aspects of cellular invasion that are often left out of in vitro assays are considerations about the 3D structural heterogeneity of the primary tumor and the ability of cells to migrate in all directions. Accordingly, we have taken advantage of the methodology previously described for 3D cell culture techniques and have developed a 3D invasion assay using cell clusters that can be used to assess the effects of different drugs and treatment conditions on cancer cell invasion. We also describe a novel whole-mount technique that permits fluorescence-based immunolocalization of proteins through the entire tumorsphere, without the need for sectioning. Our assay provides a simple, inexpensive, and physiologically relevant context to study cellular invasion in vitro, in a way that recapitulates an in vivo milieu.

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