Corrective effect of immunological drugs on oral microbiozinosis in patients with chronical apical periodontitis against the background of oral dysbiosis

2019 ◽  
Vol 24 (2) ◽  
pp. 173-177
Author(s):  
N. I. Chepurova ◽  
I. G. Romanenko
Keyword(s):  
Oral Cavity ◽  
Clinical Manifestations ◽  
Apical Periodontitis ◽  
Control Group ◽  
Immunomodulatory Drugs ◽  
Treatment Comparison ◽  
Corrective Effect ◽  
Grade Ii ◽  
Destructive Processes

Relevance. One of the main causes of periodontitis is oral dysbiosis, in which changes in the quantitative and qualitative composition of the microflora occur, the systems of interaction between the microorganism and the microbiota are disturbed, and immunity is reduced. Is to study the comparative anti-disbiotic efcacy of various immunobiological preparations in patients with chronic apical periodontitis against the background of oral dysbiosis.Materials and methods. The study included 66 patients who were divided into 2 groups: comparisons (n = 34) and main (n = 32). Each group was divided into three subgroups depending on the degree of dysbiosis of the oral cavity according to the method of A.P. Levitsky (2007): determination of the ratio of the relative activities of urease and saliva lysozyme. All patients underwent traditional endodontic treatment and oral hygiene with parallel correction of oral microbiocenosis through the use of a complex of immunomodulatory drugs to patients of the main group.Results. In chronic apical periodontitis, urease activity was signifcantly increased, and in those examined with grade II-III dysbiosis almost 2 times compared with patients with grade I severity of clinical manifestations. The proposed treatment reduced the activity of urease in patients in all three subgroups of both groups (main and comparison). The results indicate a signifcant decrease in the activity of lysozyme in all patients more than 2 times. Conducted traditional treatment (comparison group) increased activity, but did not lead to a recovery in the rate of healthy individuals. The use of immunomodulatory drugs allowed to increase the level of lysozyme to the indicators of the control group.Conclusions. The inclusion of immunobiological preparations in the complex therapy of chronic apical periodontitis against the background of oral dysbiosis eliminates the dysbiotic phenomena in the oral cavity and has a therapeutic effect on destructive processes in the periapical tissues that are difcult to treat and often recur.

scholarly journals Effect of immunoadjuvants on the disease course in patients with operable breast cancer

2019 ◽  
Vol 14 (4) ◽  
pp. 20-23
Author(s):  
M. M. Vasilyeva ◽  
I. P. Moshurov ◽  
L. Yu. Grivtsova ◽  
N. N. Tupitsyn ◽  
N. A. Kozlov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Tissue ◽  
Radical Mastectomy ◽  
Control Group ◽  
Cancer Management ◽  
New Strategy ◽  
Breast Cancer Management ◽  
Grade Ii ◽  
And Control

Background. A new strategy of breast cancer management is required for effective disease prevention and control. Immunotherapy is one of promising options.Objective: to evaluate the effect of immunoadjuvant polyoxidonium on pathmorphological changes in breast tumors.Materials and methods. The study was performed in 75 patients with breast cancer: 50 patients received polyoxidonium in a neoadjuvant regimen (main group), 25 patients did not receive polyoxidonium (control group). Polyoxidonium at a dose of 12 mg was administered intramuscularly 1 time per day on the days 1, 2, 3, 5 and 7 before surgery; on the day 8, radical mastectomy was performed in patients of both groups, followed by histological examination and determination of the grade of therapeutic pathomorphosis of the tumor.Results. The proportion of cases with grade I therapeutic pathomorphosis was 58 %, grade II therapeutic pathomorphosis – 6 %. Pathomorphosis in metastatic lymph nodes was observed in 22.7 % of cases.Conclusion. The anti-tumor effect of polyoxidonium was confirmed on the basis of the induction of pathomorphosis of the tumor tissue of breast cancer.

Effect of copper oxide nanoparticles on gene expression of NMDA receptor

2021 ◽  
Vol 29 (6) ◽  
pp. 60-66
Author(s):  
Ivan Andreevich Sitnikov ◽  
Daria Ramilevna Shaikhova ◽  
Anna Mikhailovna Amromina ◽  
Marina Petrovna Sutunkova ◽  
Yuliya Vladimirovna Ryabova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nmda Receptor ◽  
Copper Oxide ◽  
Clinical Manifestations ◽  
Copper Oxide Nanoparticles ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Genes Encoding ◽  
Effect Of Copper

Introduction. Copper plays an important role in the metabolism of the brain, but particles of copper, in the nanometer range, exhibit neurotoxic properties and cause malfunctioning of brain cells. Material and methods. For 6 weeks, 3 times a week, the animals were injected with a suspension of NPs of copper oxide. The determination of the expression of the genes GRIN1, GRIN2a, and GRIN2b, encoding the proteins GluN1, GluN2a, and GluN2b, respectively, was carried out by real-time PCR with probes. Results. A statistically significant decrease in the expression level of genes encoding NMDA receptor proteins was determined when exposed to 0.5 mg/ml CuO nanoparticles (ΔCt(GRIN1) = 0.813; ΔCt(GRIN2A) = 3.477; ΔCt(GRIN2B) = 1.37) in comparison with control group (ΔCt(GRIN1) = 6.301; ΔCt(GRIN2A) = 7.823; ΔCt(GRIN2B) = 4.747). Conclusion. Evaluation of gene expression of the NMDA receptor may be present in a genetic marker to determine the toxic effect of copper oxide nanoparticles; however, further studies are needed, including behavioral tests to confirm the clinical manifestations of neurodegenerative disorders.

scholarly journals Dental status of professional athletes

2020 ◽  
Vol 101 (3) ◽  
pp. 365-370
Author(s):  
J G Hajiyev
Keyword(s):  
Oral Cavity ◽  
Oral Hygiene ◽  
Clinical Manifestations ◽  
Maximum Level ◽  
The State ◽  
Dental Status ◽  
Professional Athletes ◽  
Control Group ◽  
Periodontal Tissues ◽  
Periodontal Inflammation

Aim. To reveal the rate and severity of clinical manifestations of common dental diseases and dentofacial anomalies in professional athletes Methods. The effect of intense physical exertion and overtraining syndrome on the state of the organs and tissues of the dental system were examined in 200 professional athletes aged 1825 years between 2014 and 2016. Five groups where each has the same number of athletes (n=40) included: team sport athletes (volleyball, handball), martial arts athletes (wrestling, boxing), gymnasts, swimmers, athletes. The control group consisted of 40 individuals who were not professionally involved in sports. The state of the oral cavity, in particular periodontal tissues, was judged by changes in the following indicators: prevalence of dentofacial anomalies, the incidence of dental caries, state of the oral cavity hygienic using by simplified oral hygiene index (OHI-S) ( Green J.С., Vermillion J.R., 1963), degree of gums bleeding by using the MuhlemannCowell bleeding index, degrees of severity of periodontopathy by using the papillary-marginal alveolar index (RMA) [Massler, Schour (1949) in Parma modification (1960)]. Results. According to the values of the simplified oral hygiene index the worst state of the oral cavity hygienic was in wrestlers (2.120.022 points) and boxers (2.030.029) compared to the control group (1.620.026, p=0.049 and p=0.001, respectively). Catarrhal gingivitis and generalized chronic periodontitis are most often diagnosed in the groups of wrestling (82.56.01%), gymnastics (77.56.60%), boxing (70.07.25%) and swimming (70.07.25%). Dentoalveolar anomalies were more often detected in boxers (77.5% of cases, p=0.001) and in gymnasts (34.0%, p=0.365) compared to the control group. In these groups, diastema was also more often diagnosed. Conclusion. Periodontal inflammation indicators were higher in wrestlers and gymnasts groups compared to the dental status of all other groups of athletes; caries and maximum level of dental anomalies are more common for boxers.

scholarly journals Yeasts from the oral cavity of children with AIDS: exoenzyme production and antifungal resistance

2003 ◽  
Vol 17 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Vera Lúcia Bosco ◽  
Esther Goldenberg Birman ◽  
Arlete Emily Cury ◽  
Claudete Rodrigues Paula
Keyword(s):  
Oral Cavity ◽  
Amphotericin B ◽  
Virulence Factors ◽  
Outpatient Treatment ◽  
Healthy Subjects ◽  
Antifungal Agents ◽  
Clinical Manifestations ◽  
Control Group ◽  
Test Kit ◽  
Fungal Microbiota

The oral fungal microbiota of 30 children with AIDS, of both genders, aged from two to six years, receiving outpatient treatment, was evaluated and compared with that of a control group composed of 30 healthy subjects with matching ages and genders. Virulence factors, such as exoenzyme production, and susceptibility to five antifungal agents using an E-Test kit were evaluated. C. albicans predominated over other species in the AIDS group, showing a higher production of proteinase and phospholipase when compared with that observed in the control group. In this study few clinical manifestations of and low selectivity for C. albicans (23.3%) were observed in the AIDS group. The enzymatic studies showed that 53.8% of the AIDS strains were strongly positive whereas only 33.3% of the non-AIDS strains were positive. Amphotericin B was the most effective drug among the antifungal agents tested against C. albicans. The frequency, selectivity and level of exoenzyme production by C. albicans suggest a higher pathogenicity in the AIDS children than in the control children.

scholarly journals CLINICAL AND DIAGNOSTIC VALUE OF DETERMINATION OF NEOPTERIN IN ATOPIC DERMATITIS IN CHILDREN

2018 ◽  
Vol 25 (3) ◽  
pp. 24-27
Author(s):  
M. M. BEN ◽  
E. YU. YANCHEVSKAYA ◽  
O. A. MESNYANKINA
Keyword(s):  
Atopic Dermatitis ◽  
Skin Diseases ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Diagnostic Value ◽  
Laboratory Method ◽  
Control Group ◽  
Allergic Skin ◽  
Monitoring And Forecasting

Aim. To improve the diagnosis of allergic skin diseases in childrenMaterials and methods. 135 children aged from 2 to 6 with atopic dermatitis were examined during exacerbation. They were on inpatient treatment in the skin and venereologic department of the Regional skin and venereologic dispensary and the Department of Allergology of the Regional children's clinical hospital. At the same time, 103 people (the main group) had uncomplicated course of the disease, 32 patients had complications of atopic dermatitis in the form of secondary infection. The control group consisted of 29 children aged 2 to 6. Neopterin in serum was determined by enzyme-linked immunosorbent analysis using test kits "Neopterin ELISA" (Germany). Statistical reliability of the obtained data was evaluated using The Student's test.Results. Atopic dermatitis in children, regardless of clinical manifestations, is accompanied by an increase in the content of neopterin in serum. The nature of the increase in the level of neopterin is determined by the duration of the disease, while its highest values are characteristic of the prolonged course of the process (over 4 years).Conclusion. The determination of neopterin in children with atopic dermatitis is a promising laboratory method for assessing the severity of dermatosis, monitoring and forecasting the further course of the process, as well as evaluating the effectiveness of therapeutic measures.

Influence of the combined application of physiotherapeutic factors on microhemodynamic parameters and the level of oxygenation of tissues of the peri-implant zone in inflammatory complications in patients with orthopedic constructions on dental implants

2021 ◽  
pp. 48-58
Author(s):  
V. I. Livshits ◽  
S. N. Nagornev ◽  
R. Sh. Gvetadze ◽  
V. K. Frolkov
Keyword(s):  
Dental Implants ◽  
Dental Treatment ◽  
Clinical Manifestations ◽  
Low Frequency ◽  
Initial State ◽  
Trophic Relations ◽  
Level Of Functioning ◽  
Combined Application ◽  
Corrective Effect ◽  
Destructive Processes

The article analyzes the role of microcirculatory disorders in the pathogenesis of inflammatory complications (peri-implantitis) in patients with orthopedic constructions on dental implants and substantiates the possibility of a complex course application of low-intensity infrared laser therapy and low-frequency electrostatic therapy. It was shown that the initial state of patients with peri-implantitis is characterized by significantly low values of the level of general perfusion and its intensity, as well as the predominance of shunt mechanisms that reduce the values of the nutritive link of blood flow. The cause of the revealed disorders is the low level of functioning of the vasodilation mechanisms, caused by inflammatory and destructive processes in the peri-implant tissues. The use of a complex of physiotherapeutic exposure was characterized by a more pronounced corrective effect on the parameters of microcirculation and oxidative metabolism in comparison with standard dental treatment. The observed dynamics of microcirculatory systems of tissues of the peri-implant zone under the conditions of the complex influence of physiotherapeutic factors is aimed at the formation of adaptive reactions of local hemodynamics to the local needs of soft and bone tissues experiencing an inflammatory process around the implant, as well as strengthening the mechanisms of long-term adaptation of trophic relations in tissue peri-implant microregions. Taking into account the pathogenetic significance of microhemodynamic disorders in the formation of clinical manifestations of peri-implantitis, the revealed activating effect of the technology of the combined use of a laser and low-frequency electrostatic therapy serves as an objective proof of its therapeutic efficacy.

Cardiac and Psycho-vegetative Abnormalities During Menopause Period and the Ways for Their Therapeutic Correction

2016 ◽  
pp. 36-40
Author(s):  
Ivan Katerenchuk
Keyword(s):  
Blood Pressure ◽  
Coronary Heart Disease ◽  
Beta Blockers ◽  
Clinical Manifestations ◽  
Control Group ◽  
Lipid Disorders ◽  
Concurrent Use ◽  
Complex Therapy

The objective: to study the features of cardiac and autonomic symptoms in women in climacteric period and determination of the effectiveness of Maksipim in the correction of violations. Patients and methods. There were examined 62 patients perimenopausal and postmenopausal period of life between the ages from 48 to 67 years (average age of 51.2±7,42 years). Women in the control group (n=32) received conventional therapy according to the standards (nitrates, beta-blockers, ACE inhibitors, statins). Women of the controlled group (n=30) on the background of basic therapy were receiving additional Maksipim (STADA, Germany) (active substance 3-hydroxy-6-methyl-2-ethylpyridine succinate) for 5 ml intravenously once a day, 5 injections, then oraly 1 tablet (0.125 g) 3 times a day for 1 month. Results. The results of these studies showed that menopause lipid disorders, clinical manifestations of angina, presence of hypertension and psycho-vegetative disorders. Conclusion. Application in complex therapy of patients with coronary heart disease (CHD) combined with arterial hypertension in the menopausal period of the drug Maksipim, helps normalize lipid metabolism, reduce the clinical manifestations of CHD, decrease of blood pressure. Concurrent use Maksipim helps to eliminate manifestations of somatic vegetative and psycho-vegetative disorders, which favorably affects the quality of patients’ life.

Determination of clinical and metabolic features in pre- and postmenopausal women with type 2 diabetes mellitus and osteoarthrosis against the background of visfatin metabolism disorders

2021 ◽  
Author(s):  
V. O. Cherpita
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Postmenopausal Women ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Women

Objective — to determine the clinical and metabolic features of pre‑ and postmenopausal women with type 2 diabetes mellitus (DM2) and osteoarthritis (OA) against the background of impaired metabolism of visfatin (VF). Materials and methods. 120 pre‑ and postmenopausal women were selected for the study and divided into 3 groups: group 1 included women with isolated DM2; group 2 — women with isolated OA; group 3 — women with DM2, combined with OA. The control group consisted of 16 healthy women. Investigations included anthropometric measurements, assessment of the indices of lipid and carbohydrate exchange and clinical manifestations of premenopause and postmenopause. Determination of the serum VF levels of patients was performed by enzyme‑linked immunosorbent assay on the analyzer «Labline‑90» (Austria). Results. Investigation of the specific features of the DM2 and OA course in premenopausal women demonstrated violations of carbohydrate and lipid metabolism, defined by the significant (р < 0.001) increase in VF levels up to (3.9 ± 1.2 ng/ml) and (4.2 ± 1.1 ng/ml), respectively. The highest VF level was recorded in the group of comorbid DM2 and OA (5.5 ± 1.0 ng/ml) compared with the levels of relatively healthy women of the same age group (1.8 ± 0.5 ng/ml). Moreover, the high indices of body mass index, waist and hip circumference, systolic and diastolic pressure, as well as of the menopausal index were established at DM2 and OA vs control group. Conclusions. Clinical and metabolic disorders have been identified in pre‑ and postmenopausal women against the background of OA and DM2 based on the significant (р < 0.001) increase in visfatin levels, especially in case of comorbid OA and DM2, as compared to the control group of age‑matched practically healthy women.

CLINIC-MOLECULAR INDICATORS OF INFLAMMATORY DESTRUCTIVE DAMAGE OF THE ORAL CAVITY IN PERIODONTITIS IN PERSONS WITH VARIOUS GROUP ACCESSORIES OF BLOOD

2020 ◽  
Vol 65 (2) ◽  
pp. 100-105
Author(s):  
I. A. Selezneva ◽  
F. N. Gilmiyarova ◽  
I. A. Borodina ◽  
A. A. Ereshchenko ◽  
E. M. Gilmiyarov ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Blood Group ◽  
Oral Fluid ◽  
Clinical Manifestations ◽  
Similar Data ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Inflammatory Processes ◽  
Destructive Processes ◽  
Molecular Indicators

In order to find a connection between the alteration of oral tissues and genetic predisposition to inflammatory and destructive processes in oral media, the cytokine profile of the oral fluid of clinically healthy individuals was determined for various blood group affiliations according to the AB0 system. The group-specific features of individuals with B(III) blood group were revealed: an increase of 32,5% in the content of interleukin-6 and 63,1% in the content of interleukin-8 compared with similar data for people with 0(I), A(II), AB(IV) blood groups, which can predispose to the greatest activity of the inflammatory process in the oral cavity in individuals with antigen B. Confirmation of this fact is an increase of IgA antibodies to gliadin in the blood among patients with chronic generalized periodontitis with B(III) blood group, up to 5,00 U/ml (p<0,01), which indicates the processes of acute inflammation, and along with an increase in blood IgG antibodies to transglutaminase in comparison with a group of clinically healthy individuals, it serves as an indicator of damage to the body’s connective tissue at the molecular level. When examining the dental status, pronounced clinical manifestations of chronic generalized periodontitis were found in patients with A(II) blood group, the molecular foundation of which is the highest content of IgA and IgG antibodies to transglutaminase in the oral fluid (0,35 U/ml and 0,45 U/ml), which contributes to the activation of periodontal-destroying inflammatory and inflammatory processes, obviously, with a tendency to the chronic course of the disease. The studies performed allowed us to analyze in clinically healthy individuals a predisposition to alternative processes in oral environments, using gradation by group blood affiliation.

scholarly journals MODERN DIAGNOSTICS METHODS OF NON-SPECIFIC PROTECTION OF PERIODONTAL TISSUES IN RESIDENTS OF THE INDUSTRIAL REGION

2020 ◽  
pp. 18-23
Author(s):  
A.V. Samoilenko ◽  
S.V. Pavlov ◽  
I.V. Vozna
Keyword(s):  
Oral Cavity ◽  
Oral Fluid ◽  
Pathological Process ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Study Group ◽  
Periodontal Tissue ◽  
Oral Liquid ◽  
Healthy Control

The aim of the article is to study the peculiarities of antimicrobial immunity of the oral cavity of the industrial region’s inhabitants in order to optimize special preventive programs of major dental diseases. The object and research methods. 178 patients was examined from 21 to 50 years old, who turned to the University Dental Center of Zaporizhzhia State Medical University. The study group consisted of 126 patients with generalized periodontitis of the initial (8), I (32), II (68) and III (18) degree of chronic course, complicated by harmful factors of manufacturing. The comparison group consisted of 32 patients with periodontitis (5), I (10), II (11) and III (6) degrees who did not work in adverse conditions. The control group consisted of 20 relatively healthy individuals aged from 19 to 25 years without signs of generalized periodontitis. To study the factors of local immunity of the oral cavity, oral fluid was collected from each examined person, which was obtained without stimulation, spitting into sterile tubes. Then the oral fluid was centrifuged for 15 minutes at 8,000 rpm. The supernatant part of the oral liquid was poured into plastic tubes and stored at 30°C. The quantitative determination of markers in the oral fluid was performed by standard enzyme immunoassay kits according to the instructions of the "Lactoferrin-strip" ("VectorBest") manufacturers. The result was expressed in mµg / ml. The content of cathelicidin LL-37 was determined by enzyme-linked immunosorbent assay using NycultBiotechhuman LL-37 ELISA (Netherlands) firm reagent kit-manufacturers. The result was expressed in µg / ml. Statistica 13.0 licensed number JPZ804I382130ARCN10-J was used to process the results. The results of the study and their discussion. The hygienic condition in the examined patients of the study group was assessed as unsatisfactory: from 2.24 ± 0.5 to 2.99 ± 0.47 points depending on the age and length of service at the enterprise, but the PI and SBI values were increased. It was found, that the lactoferrin level in the oral fluid was higher in the all patients with periodontitis, than the same index in comparison with healthy control group and consistently increased with increasing severity of the process. In the patients’ study group, working in hazardous conditions of production, the lactoferrin content has increased in the oral fluid relative to the level of the healthy patients was more pronounced in comparison with the patients, suffering of periodontal tissue disease, but do not work in harmful manufacturing conditions. Increased lactoferrin in the oral fluid in the steelmaking workers can be considered as a means of compensation, that provides protection of the oral mucosa from colonization of microorganisms. In the steel industry workers, a statistically significant cathelicidin content decrease in the oral fluid was observed compared to healthy control patients’ group. In the clinical group of the patients without adverse factors of production, the cathelicidin’s concentration in the oral fluid was also reduced. The increasing level of lactoferrin in the oral fluid in parallel with the cathelicidin’s decrease in saliva are markers of the inflammatory phase, as well as the destructive phase of connective tissue. Conclusions. Thus, our observations have established a direct relationship between the lactoferrin’s concentration in the oral fluid and the severity of periodontal tissue diseases and the feedback between the cathelicidin’s content in the oral liquid and the activity of the pathological process. The determination of lactoferrin and cathelicidin in the oral fluid of the patients with harmful manufacturing factors allow us to identify the signs of pathological process in the oral cavity.

Sign in / Sign up

Export Citation Format

Share Document