Identification of a β1 integrin isoform with restricted tissue expression in a teleost fish

The composition and organisation of extracellular matrix (ECM)-related molecules change during development. These components interact with different cell surface receptors to modulate the transduction of signals for cell growth, differentiation, migration, proliferation and apoptosis. Previous findings in the teleost fish gilthead seabream (Sparus aurata L., Teleostei), a marine protandrous hermaphrodite fish, showed that endocrine and immune stimuli are able to modulate the expression of ECM-related molecules, as well as specific correlations between them. In the present study, quantitative reverse transcription–polymerase chain reaction was used to examine the gene expression profile of β1 integrin isoform b (ITGB1b) and its possible role in reproductive physiology, especially in relation to spermatogenesis. Expression profiles were analysed in the context of the reproductive cycle (RC) and in relation with other ECM-related molecules, including matrix metalloproteinase (MMP)-2, MMP-9, MMP-13, tissue-specific inhibitor of metalloproteinase (TIMP)-2a, TIMP-2b, collagen (COL1A1) and ITGB1a. Expression of ITGB1b was found in the testis and brain and, to some extent, in endothelial cells. In contrast, ITGB1a was expressed ubiquitously. In the testis, the ITGB1b expression peaked during spermatogenesis, whereas the expression of the other ECM-related molecules is induced mainly during the post-spawning stage, both stages of marked tissue remodelling during the first and second RC in males. In addition, in fish exposed to the endocrine disruptor 17α-ethynyloestradiol (at 5 and 50 μg g–1 food during 7, 14 and 21 days), ITGB1b expression in the testis was inhibited in a dose- and time-dependent manner and was related to reduced serum levels of testosterone. Together, these results suggest a different functionality for the two ITGB1 isoforms in the gilthead seabream, where ITGB1b is more specifically involved in reproduction. This is the first report of an ITGB1 gene isoform whose expression is restricted to endocrine-related tissues in vertebrates.

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Patrón de expresión tisular de los genes elovl4 en Sparus aurata y Solea senegalensis: de larva a adulto

AquaTechnica: Revista Iberoamericana de Acuicultura. ◽

10.33936/at.v3i1.3476 ◽

2021 ◽

Vol 3 (1) ◽

pp. 16

Author(s):

Miguel Torres-Rodríguez ◽

Francisco Hontoria Danés ◽

Óscar Monroig Marzá ◽

Inmaculada Varó Vaello ◽

Juan Carlos Navarro Tárrega

Keyword(s):

Fatty Acids ◽

Expression Pattern ◽

Sparus Aurata ◽

Tissue Expression ◽

Development Stage ◽

Solea Senegalensis ◽

Gilthead Seabream ◽

Tissue Expression Pattern ◽

Neural Tissues ◽

Long Chain

Very long-chain (˃ C24) fatty acids (VLC-FA) play critical roles during early development of vertebrates, since these compounds are accumulated in the rapidly forming neural tissues, ensuring their normal function. The functionality of VLC-FA has aroused scientific interest, focusing on the study of elongases protein 4 of very long chain fatty acid (Elovl4), which are responsible for their biosynthesis from shorter fatty acids (precursors). For a better understanding of the metabolism and the potential tissue-specific requirements of VLC-FA in marine teleosts, the present study aimed to determine the tissue-expression pattern of the genes that encode for Elovl4 isoforms, i.e. elovl4a and elovl4b, in different windows of development (larval and adult stages) of Gilthead seabream Sparus aurata and Senegalese sole Solea senegalensis. The results indicated that in S. aurata larvae, elovl4a is widely expressed in the head, while elovl4b is strongly focused in the eyes. Interestingly, in S. senegalensis larvae an opposite tissue-expression pattern was observed for both elovl4 isoforms. In adults of both fish, although elovl4 transcripts were detected in most tissues analyzed, elovl4a and elovl4b genes were strongly expressed in brain and eyes, respectively. Importantly, the differential tissue-expression pattern of both elovl4 isoforms associated to the pre- and post-metamorphic stage of S. senegalensis could be indicative of the VLC-FA particular needs linked to neural tissues functionality in each development stage. These findings can contribute to a better understanding of the species-specific VLC-FA metabolism in marine teleosts.

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Toll-like receptor 22 of gilthead seabream, Sparus aurata: Molecular cloning, expression profiles and post-transcriptional regulation

Developmental & Comparative Immunology ◽

10.1016/j.dci.2013.12.003 ◽

2014 ◽

Vol 44 (1) ◽

pp. 173-179 ◽

Cited By ~ 22

Author(s):

Iciar Muñoz ◽

María Pilar Sepulcre ◽

José Meseguer ◽

Victoriano Mulero

Keyword(s):

Transcriptional Regulation ◽

Molecular Cloning ◽

Sparus Aurata ◽

Expression Profiles ◽

Gilthead Seabream ◽

Toll Like Receptor ◽

Post Transcriptional Regulation

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Identification of a FasL-like molecule in leucocytes of the teleost fish gilthead seabream (Sparus aurata L.)

Developmental & Comparative Immunology ◽

10.1016/s0145-305x(02)00041-1 ◽

2003 ◽

Vol 27 (1) ◽

pp. 21-27 ◽

Cited By ~ 20

Author(s):

Alberto Cuesta ◽

M Ángeles Esteban ◽

José Meseguer

Keyword(s):

Teleost Fish ◽

Sparus Aurata ◽

Gilthead Seabream

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Bortezomib-Induced Reversal of CAM-DR Phenotype in Myeloma Is Associated with AKT and ERK1/2 Signaling.

Blood ◽

10.1182/blood.v104.11.3357.3357 ◽

2004 ◽

Vol 104 (11) ◽

pp. 3357-3357

Author(s):

Ramadevi Nimmanapalli ◽

Lori Hazlehurst ◽

Steven Enkemann ◽

Melissa Alsina ◽

William S. Dalton

Keyword(s):

Multiple Myeloma ◽

Drug Resistance ◽

Tumor Cell ◽

Expression Profiles ◽

Specific Inhibitor ◽

Gene Expression Profiles ◽

Akt Signaling ◽

Chemotherapeutic Agents ◽

Dependent Manner ◽

Myeloma Cells

Abstract An increasing body of evidence suggests that interactions between myeloma cells and the microenvironment are critical for tumor cell survival, proliferation and drug resistance. These pro-survival interactions include binding of soluble factors, and direct contact with stromal cells, or extra cellular matrix (ECM) components such as fibronectin (FN). More specifically, adhesion of multiple myeloma (MM) to FN via ν1 integrins has been demonstrated to confer resistance to a host of chemotherapeutic agents (Shain et al., 2000). This anti-apoptotic phenomenon, termed cell adhesion-mediated drug resistance (CAM-DR), is an intrinsic pathway for evading the therapy-induced apoptosis (Damiano et al., 2000). Bortezomib (PS-341), a specific inhibitor of proteosome, has been shown to cause apoptosis of several tumor cell lines including multiple myeloma. In contrast to conventional cytotoxics, Bortezomib, showed more apoptosis in 24 hours for 8226 myeloma cells adhered to FN (25 % 10 nM) compared to cells in suspension (10 % 10 nM) in a dose -dependent manner. To investigate the signal transduction mechanism responsible for the reversal of CAM-DR phenotype by Bortezomib, we conducted Affymetrix GeneChipÒ Expression of 8226 cells cultured either in suspension, or adhered to FN in the presence and absence of Bortezomib (50 nM) for 4 and 24 hours using the Affymetrix U133A chips. A total of 197 genes were upregulated on FN compared to suspension, among which only 12 genes were increased with Bortezomib and 105 genes were decreased with PS-341. A total of 870 genes were downregulated on FN adhesion, among which the majority of them were increased with Bortezomib. Similar patterns of gene expression profiles were observed after 24 hrs of treatment with Bortezomib. To examine how Bortezomib is reversing CAMDR phenotype, we chose to examine the genes that were changed with FN adhesion and reversed when treated with Bortezomib. The adhesion of 8226 cells to FN enhanced both ERK1/2 and AKT signaling pathways, and both pathways were down regulated when treated with Bortezomib. The main indicators of the AKT signaling on FN are upregulation of PYK2, AKT2, MDM2 and downregulation of p21 and BAD. In conformation with our micro-array data, PYK2 phosphorylation and ERK1/2 phosphorylation were enhanced when 8226 cells were adhered to FN. The mechanisms to understand role of PYK2 phosphorylation and downstream AKT signaling in Bortezomib-induced reversal of CAMDR are under investigation.

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The molecular, functional and phylogenetic characterization of PGE2 receptors reveals their different roles in the immune response of the teleost fish gilthead seabream (Sparus aurata L.)

Developmental & Comparative Immunology ◽

10.1016/j.dci.2020.103803 ◽

2021 ◽

Vol 114 ◽

pp. 103803

Author(s):

Fatma Hermi ◽

Victoria Gómez-Abellán ◽

Ana B. Pérez-Oliva ◽

Diana García-Moreno ◽

Azucena López-Muñoz ◽

...

Keyword(s):

Immune Response ◽

Teleost Fish ◽

Sparus Aurata ◽

Gilthead Seabream ◽

Phylogenetic Characterization

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The compositional and metabolic responses of gilthead seabream (Sparus aurata) to a gradient of dietary fish oil and associatedn-3 long-chain PUFA content

British Journal Of Nutrition ◽

10.1017/s0007114517002975 ◽

2017 ◽

Vol 118 (12) ◽

pp. 1010-1022 ◽

Cited By ~ 22

Author(s):

Sam J. S. Houston ◽

Vasileios Karalazos ◽

John Tinsley ◽

Mónica B. Betancor ◽

Samuel A. M. Martin ◽

...

Keyword(s):

Fatty Acid ◽

Fish Oil ◽

Fatty Acid Synthase ◽

Sparus Aurata ◽

Gilthead Seabream ◽

Dependent Manner ◽

Tissue Fatty Acid ◽

Chain Pufa ◽

Fat Deposits ◽

Long Chain

AbstractThe replacement of fish oil (FO) with vegetable oil (VO) in feed formulations reduces the availability ofn-3 long-chain PUFA (LC-PUFA) to marine fish such as gilthead seabream. The aim of this study was to examine compositional and physiological responses to a dietary gradient ofn-3 LC-PUFA. Six iso-energetic and iso-nitrogenous diets (D1–D6) were fed to seabream, with the added oil being a blend of FO and VO to achieve a dietary gradient ofn-3 LC-PUFA. Fish were sampled after 4 months feeding, to determine biochemical composition, tissue fatty acid concentrations and lipid metabolic gene expression. The results indicated a disturbance to lipid metabolism, with fat in the liver increased and fat deposits in the viscera reduced. Tissue fatty acid profiles were altered towards the fatty acid compositions of the diets. There was evidence of endogenous modification of dietary PUFA in the liver which correlated with the expression of fatty acid desaturase 2 (fads2). Expression of sterol regulatory element binding protein 1 (srebp1), fads2and fatty acid synthase increased in the liver, whereas PPARα1 pathways appeared to be supressed by dietary VO in a concentration-dependent manner. The effects in lipogenic genes appear to become measurable in D1–D3, which agrees with the weight gain data suggesting that disturbances to energy metabolism and lipogenesis may be related to performance differences. These findings suggested that suppression ofβ-oxidation and stimulation ofsrebp1-mediated lipogenesis may play a role in contributing toward steatosis in fish fedn-3 LC-PUFA deficient diets.

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Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/5649169 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Ruihua Zhong ◽

Aying Ma ◽

Jianping Zhu ◽

Guoting Li ◽

Shuwu Xie ◽

...

Keyword(s):

Cytochrome C ◽

Protein Expression ◽

Rat Model ◽

Expression Profiles ◽

Serum Levels ◽

Dependent Manner ◽

Western Blot Assay ◽

Eutopic Endometrium ◽

Protein Levels ◽

Ectopic Endometrium

We evaluated the effectiveness of Kuntai Capsule (KTC) for treating endometriosis using rat model and investigated its preliminary mechanism of action involved. SD rats were implanted with endometrial tissues and treated with KTC for three weeks. Then, laparotomy was performed to examine volume changes of the autografts. The serum levels of TNF-α, IL-6, COX-2, E2, and P4 were measured through ELISA. TUNEL was performed to analyze the apoptosis on ectopic endometrium. Protein levels of caspases 8, 9, and 3 and cytochrome c in the ectopic and eutopic endometrium were measured by western blotting. Results showed that KTC significantly decreased the volumes of ectopic endometrium. The level of TNF-αincreased and E2decreased in the KTC treatment groups. TUNEL and western blot assay showed that KTC could induce apoptosis of endometriotic tissues, accompanied with the increased protein expression of caspases 8 and 9, activated caspase-3, and cytochrome c in a dose-dependent manner. However, these protein expression profiles were not affected in eutopic endometrium. Our findings suggest that KTC could inhibit the growth of ectopic endometrial tissue through upregulating the level of TNF-αand its downstream signaling, including caspases and cytochrome c.

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Vitamin D Supplementation and Serum Levels of Magne-sium and Selenium in Type 2 Diabetes Mellitus Patients: Gender Dimorphic Changes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000190 ◽

2014 ◽

Vol 84 (1-2) ◽

pp. 27-34 ◽

Cited By ~ 7

Author(s):

Nasser M. Al-Daghri ◽

Khalid M. Alkharfy ◽

Nasiruddin Khan ◽

Hanan A. Alfawaz ◽

Abdulrahman S. Al-Ajlan ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Vitamin D ◽

Type 2 Diabetes Mellitus ◽

Serum Levels ◽

Vitamin D Supplementation ◽

Serum Selenium ◽

Dependent Manner

The aim of our study was to evaluate the effects of vitamin D supplementation on circulating levels of magnesium and selenium in patients with type 2 diabetes mellitus (T2DM). A total of 126 adult Saudi patients (55 men and 71 women, mean age 53.6 ± 10.7 years) with controlled T2DM were randomly recruited for the study. All subjects were given vitamin D3 tablets (2000 IU/day) for six months. Follow-up mean concentrations of serum 25-hydroxyvitamin D [25-(OH) vitamin D] significantly increased in both men (34.1 ± 12.4 to 57.8 ± 17.0 nmol/L) and women (35.7 ± 13.5 to 60.1 ± 18.5 nmol/L, p < 0.001), while levels of parathyroid hormone (PTH) decreased significantly in both men (1.6 ± 0.17 to 0.96 ± 0.10 pmol/L, p = 0.003) and women (1.6 ± 0.17 to 1.0 ± 0.14 pmol/L, p = 0.02). In addition, there was a significant increase in serum levels of selenium and magnesium in men and women (p-values < 0.001 and 0.04, respectively) after follow-up. In women, a significant correlation was observed between delta change (variables at six months-variable at baseline) of serum magnesium versus high-density lipoprotein (HDL)-cholesterol (r = 0.36, p = 0.006) and fasting glucose (r = - 0.33, p = 0.01). In men, there was a significant correlation between serum selenium and triglycerides (r = 0.32, p = 0.04). Vitamin D supplementation improves serum concentrations of magnesium and selenium in a gender-dependent manner, which in turn could affect several cardiometabolic parameters such as glucose and lipids.

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Changes in growth rate and thyroid- and sex-hormones blood levels in rats under sub-chronic lithium treatment

Human & Experimental Toxicology ◽

10.1191/0960327106ht620oa ◽

2006 ◽

Vol 25 (5) ◽

pp. 243-250 ◽

Cited By ~ 9

Author(s):

M S Allagui ◽

N Hfaiedh ◽

C Vincent ◽

F Guermazi ◽

J-C Murat ◽

...

Keyword(s):

Growth Rate ◽

Lithium Treatment ◽

Lithium Carbonate ◽

Serum Levels ◽

Antagonistic Effect ◽

Blood Levels ◽

Vaginal Mucosa ◽

Dependent Manner ◽

Serum Concentrations ◽

Dose Dependent

Lithium therapy, mainly used in curing some psychiatric diseases, is responsible for numerous undesirable side effects. The present study is a contribution to the understanding of the pathophysiological mechanisms underlying lithium toxicity. Male and female mature rats were divided into three batches and fed commercial pellets: one batch was the control and the second and third batches were given 2 g (Li1) and 4 g (Li2) of lithium carbonate/kg of food/day, respectively. After 7, 14, 21 and 28 days, serum levels of free tri-iodothyronine (FT3), thyroxine (FT4), testosterone and estradiol were measured. Attention was also paid to growth rate and a histological examination of testes or vaginal mucosa was carried out. In treated rats, a dose-dependent loss of appetite and a decrease in growth rate were observed, together with symptoms of polydypsia, polyuria and diarrhea. Lithium serum concentrations increased from 0.44 mM (day 7) to 1.34 mM (day 28) in Li1 rats and from 0.66 to 1.45 mM (day 14) in Li2 rats. Li2 treatment induced a high mortality after 14 days, reaching 50-60% in female and male animals. From these data, the LD50 (14 days Li2 chronic treatment) was calculated to be about 0.3 g/day per kilogram of animal, leading to Li serum concentrations of about 1.4 mM. A significant decrease of FT3 and FT4 was observed in treated rats. This effect appeared immediately for the highest dose and was more pronounced for FT3, resulting in an increase of the FT4/FT3 ratio. In males, testosterone decreased and spermatogenesis was stopped. Conversely, in females, estradiol increased in a dose-dependent manner as the animals were blocked in the diestrus phase at day 28. This finding supports a possible antagonistic effect of lithium on the estradiol receptors.

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