scholarly journals MILK OF BLACK-WHITE COWS WITH DIFFERENT GENES OF CAPPA CASEIN AND DIACYLGLYCEROL OF O-ACYLTRANSFERASE FOR CHEESEMAKING

2020 ◽  
Vol 5 (2) ◽  
pp. 59-63
Author(s):  
Radik Shaydullin ◽  
Gazimzyan Sharafutdinov ◽  
Anastasiy Moskvicheva

The purpose of the research is improving the quality and technological properties of milk from black-and-white cows. The research was conducted on first-calf cows of a black-and-white breed in LLC «Dusym» of the Atninsky district of the Republic of Tatarstan. Three groups of experimental cows were formed depending on the genotype of CSN3 and DGAT1, which have equal lactation month. Cheese making milk obtained from cows with different allelic variants of the kappa-casein and diacylglycerol O-acyltransferase genes was studied. DNA testing was performed on the CSN3 and DGAT1 genes of experimental cows and allelic variants of these genes were determined by pol-ymerase chain reaction followed by analysis of restriction-site polymorphism. It was found that milk of cows with the genotype CSN3 AB and CSN3 BB, when exposed to rennet, showed a better yield of a dense casein clot (80 and 100%) and separation of serum (P <0.05), with a shorter coagulation time (20.3 and 16.5 min; P <0.05-0.01). Ac-cording to the DGAT1 gene, first-calf milk with the allelic variant DGAT1 AK and DGAT1 KK had the advantage, respectively, 86.7% and 85.7%, 24.0 and 25.7 minutes. The worst milk coagulation time is typical for CSN3 AA group – 29.5 minutes and DGAT1 AA one – 30.4 min. In terms of milk coagulation time, type II (15-40 min.) groups have the genotype CSN3 AB and CSN3 BB with the largest share of cows in the herd – 80.0 and 71.4% and with the genotype DGAT1 AK and DGAT1 KK – 86.6 and 85.7%, respectively. Thus, milk obtained from cows with the kappa-casein gene allele B and the diacylglycerol O-acyltransferase gene allele K gene in the genotype has the best cheese-making properties.

Author(s):  
Р.Р. ШАЙДУЛЛИН ◽  
А.Б. МОСКВИЧЕВА ◽  
Т.Х. ФАИЗОВ ◽  
Л.Р. ЗАГИДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
...  

Изучен полиморфизм и определена частота встречаемости аллельных вариантов по генам пролактина (PRL) и соматотропина (GH) у коров в условиях двух племенных хозяйств Республики Татарстан. Объектом исследования были животные черно-пестрой породы первого (n=151) и третьего (n=168) отелов и холмогорской породы первого (n=160) и третьего (n=143) отелов. Проведено генотипирование коров по генам PRL и GH методом ДНК-диагностики. Установлено, что среди молочного скота преимущественно встречаются особи с генотипом PRL АА (70,0—77,5%), с частотой аллеля А гена пролактина — 0,83—0,87, аллеля В — 0,13—0,17. Встречаемость аллеля В была выше среди холмогорских коров (0,16—0,17). В стаде полновозрастных животных черно-пестрой породы имеется сдвиг генетического равновесия в сторону генотипов PRL AA и PRL BB (χ2=3,97; Р<0,05). В исследуемых стадах крупного рогатого скота выражено преимущество генотипа GH LL с частотой встречаемости 51,0—79,2%. Встречаемость генотипа GH VV у черно-пестрого скота составила 2,0—2,4%, у холмогорского — 5,6—7,0%. Аллель L гена соматотропина преобладает над аллелем V в обеих породах. При этом чаще встречаемость аллеля L GH среди коров холмогорской породы (0,28—0,29). У холмогорских коров больше представлены редкие и желательные аллели генов PRL и GH. Чтобы повысить встречаемость «предпочтительных» в хозяйственном отношении генотипов и аллелей ДНК-маркеров, необходимо в большей степени использовать быков-производителей с желательными аллелями PRL и GH, а именно В и V соответственно. The polymorphism was studied and the frequency of occurrence of allelic variants for the genes of prolactin (PRL) and somatotropin (GH) was determinedin cows in two bred livestock farms of the Republic of Tatarstan. The object of the study were animals of the black-and-white breed of the first (n=151) and third (n=168) calving and the Kholmogory breed of the first (n=160) and third (n=143) calving. Genotyping of cows for PRL and GH genes was carried out using method of DNA diagnostics. It was revealed that there were mainly individuals with the PRL AA genotype (70,0–77,5%)among dairy cattle, the frequency of the A allele of the prolactin gene was 0,83–0,87, the B allele was 0,13–0,17. The frequency of allele B was higher among Kholmogory cows (0,16–0,17). There was a shift in the genetic balance towards the PRL AA and PRL BB genotypes (χ2=3,97; Р<0,05) in the herd of full-age animals of the black-and-white breed. The advantage of the GH LL genotype was expressed with a frequency of occurrence 51,0–79,2% in the studied cattle herds. The occurence of the GH VV genotype was 2,0–2,4%in black-and-white cattle, 5,6–7,0% was in Kholmogorycattle. The L allele of the somatotropin gene predominated over the V allele in both breeds. Moreover, the occurrence of the L GH allele among cows of the Kholmogory breed (0,28–0,29) was more often. Rare and desirable alleles of the PRL and GH genes are more represented in Kholmogory cows. In order to increase the occurrence of economically “preferred” genotypes and alleles of DNA markers, it is necessary to use more stud bulls with the desired PRL and GH alleles, namely B and V, respectively.


2020 ◽  
Vol 11 (2) ◽  
pp. 1511-1514
Author(s):  
Sergey Yu. Smolentsev ◽  
Galiya R Yusupova ◽  
Nikita V Nikolaev ◽  
Zaira F Aukhadieva ◽  
Renat A Volkov ◽  
...  

The effectiveness of dairy cattle breeding largely depends on the intensity of use of the breeding stock. At the same time, the factor of the duration of economic use of animals is gaining importance, which affects not only the production economy, but also the improvement of stocks and breeds. The lifelong productivity, the quantitative and qualitative growth of the stock, the amount of capital investment in its formation and the efficiency of operation depend on the term for the productive use of cows. The terms of breeding and production use of animals are direct linked with the biologically possible longevity of each animal, in turn, the duration of the productive period of cattle is in the range of 12-17 lactations. The influence of feed on production is associated primarily with the level of digestion and assimilation. The digestive capacity of the digestive tract of animals can be improved by selecting the optimal ratio in the diet of rough, juicy and concentrated feeds, i.e. optimization of animal feeding types. We conducted an analysis of milk productivity in lactating cows, when using AVMC feed additive in the diet. The experiment was conducted in the conditions of the dairy farm of Biryuli CJSC of the Vysokogorsky District of the Republic of Tatarstan on three groups of lactating cows. For the experiment, 30 cows were selected, 10 in each Holstein black-and-white breed according to the principle of analogues. Studies have shown that AVMC with rapeseed oil seeds and AVMC with fus in the diet of lactating cows provided higher animal productivity and positively affected the chemical composition of milk.


2021 ◽  
pp. 19-23
Author(s):  
Т.В. Павлова ◽  
Н.Н. Шамич ◽  
В.А. Бельцов

Цель работы – установить влияние генотипа и интенсивности формирования коров на уровень их молочной продуктивности в одном из предприятий Минской области Республики Беларусь. Объектом исследований являлись 940 коров белорусской чёрно-пёстрой породы с разной долей генотипа по голштинской породе. Для проведения группировки коров по генотипам у каждого животного определяли линейную принадлежность, породность по голштинской породе и страну селекции отца. При оценке молочной продуктивности коров учитывались следующие показатели: удой за 305 дней первой и последней законченной лактации; массовая доля жира, %; массовая доля белка, %; выход молочного жира и белка, кг. Установлено, что с увеличением породности по голштинской породе у коров в стаде рассматриваемого предприятия прослеживается повышение удоев и снижение жирно- и белковомолочности. Наиболее высокий удой наблюдается у коров шведской (9190 кг) и итальянской (8148 кг) селекций, удой этих коров достоверно (Р = 0,999) превышает средний по стаду на 2019 и 977 кг соответственно; массовая доля жира и белка в молоке – у коров российской селекции (4,26 и 3,54% соответственно); у животных белорусской селекции удой составил 6714 кг при жирно- и белковомолочности 4,24 и 3,49% соответственно. С увеличением интенсивности формирования животных снижается возраст первого осеменения и увеличивается живая масса при первом осеменении, наиболее близки к оптимальным параметры у умеренноформирующихся животных. У них же отмечен и наиболее высокий удой за 305 дней первой лактации – 7226 кг, что достоверно (Р = 0,95) превышает этот показатель у медленно- и быстроформирующихся животных на 264 и 150 кг соответственно. The purpose of the work is to establish the influence of the genotype and intensity of cows’ formation on the level of their lactation performance in one of the enterprises of the Minsk region of the Republic of Belarus. The object of research was 940 cows of the Belarusian Black-and-White breed with different genotype proportions for the Holstein breed. To group cows by genotypes, each animal was determined by directional affiliation, race by Holstein breed and country of father selection. When assessing the lactation performance of cows, the following indicators were taken into account: yield for 305 days of the first and last completed lactation; weight fraction of fat, %; weight fraction of protein, %; milk fat and protein yield, kg. It was established that with an increase in breeding in the Holstein breed, cows in the herd of the enterprise under consideration show an increase in yields and a decrease in fat and protein milking capacity. The highest yield is observed in cows of Swedish (9190 kg) and Italian (8148 kg) selection, the yield of these cows significantly (P = 0.999) exceeds the herd average yield for 2019 and 977 kg, respectively; mass fraction of fat and protein in milk – in cows of Russian selection (4.26 and 3.54%, respectively); in animals of Belarusian selection the yield was 6714 kg with fat and protein milking capacity of 4.24 and 3.49%, respectively. As the intensity of animal formation increases, the age of the first insemination decreases and the live weight increases at the first insemination, the parameters are closest to optimal in moderately forming animals. They also had the highest yield for 305 days of the first lactation – 7226 kg, which significantly (P = 0.95) exceeds this indicator in slow and fast-forming animals by 264 and 150 kg, respectively.


Author(s):  
A. S. Durov ◽  
V. S. Deeva

The assessment of black-and-white, red steppe, and Simmental cattle, differentiated by milk fat was carried out. Three groups were formed: selection, production and marriage. The selection parameters are calculated using the standard deviation. The calculated parameters of selection of animals of the breeding group for milk fat in adult black-and-white cows are at least 179 kg, red steppe - 186, Simmental breed from the Novosibirsk region - 143, the Republic of Khakassia - 176 kg. Cows of the red steppe breeding group are leading in productivity. Their milk fat index is 216.8 kg (P ≥ 0.95). In animals of the black-and-white breed, the milk fat productivity is 193.2 kg. In the Simmental Republic of Khakassia it is 193.8, in the Novosibirsk region - 163.5 kg. Analysis of interbreed differences in cows of breeding groups shows that animals of the black-and-white breed surpass their peers in chest width behind the shoulder blades, milk yield and milk production index. Animals of the red steppe breed are the best in milk fat, live weight, width in shanks, oblique body length, oblique rear length, chest girth, milk fat content. Simmental cows bred in the Novosibirsk region lead over their peers in height at the withers, and Simmental cows in Khakassia - in height at the sacrum, chest depth, cannon girth, assessment of the exterior. Evaluation of the production groups of animals for milk fat allows to note that the selection group of the black-and-white breed, despite the superiority in milk yield, is inferior to the peers of the red steppe and Simmental in terms of the evaluated character. However, the cows of the black-and-white breed of the production group surpass their peers in milk fat and most other signs. The formation of breeding groups for milk fat enables to note the consolidation of the black-and-white breed in terms of productive and exterior characteristics. The Red Steppe and Simmental breeds have a high potential for improvement. These breeding groups are worthy of competing with the contemporaries of the black-andwhite breed in milk fat productivity.


2021 ◽  
pp. 33-36
Author(s):  
K. Zh. Zhumanov ◽  
T. N. Karymsakov ◽  
M. A. Kineev ◽  
A. D. Baimukanov

The relevance of research. Currently the methodological base for assessing the breeding qualities of bulls-producers of dairy and milk-meat breeds by the quality of offspring is carried out in accordance with the Instruction approved by the MA of the Republic of Kazakhstan in 2007. The principle of assessment, set out in the Instruction, is to compare the phenotypic indicators of the offspring with each other according to the principle of “peer daughter”. Although this document was approved already in the XXI century, nevertheless the basic approaches, which were put the basis, were developed in the first half of the last century and currently do not correspond to modern scientific principles. At the same time the world leaders in the field of breeding in dairy cattle breeding have been successfully using the BLUP method in breeding practice to assess the breeding qualities of animals for decades. This principle of assessing the breeding value of bulls is the most theoretically grounded and allows you to obtain results comparable to each other. Therefore the development and optimization of the equations of mixed BLUP models is extremely relevant for the conditions of the Republic of Kazakhstan from both scientific and practical points of view.Material and research methods. The material of the research was the data on the phenotypic indicators of the signs of milk productivity of first-calf cows of the Holstein black-and-white breed obtained from the republican database of the Republic of Kazakhstan for 2016-2017. As a criterion for choosing the best equation the residual variance values of each model under study were used.Results: when improving the method for assessing bulls-sires of the Holstein black-and-white breed according to the quality of offspring, out of the four studied equations of the mixed BLUP model one equation was optimized to assess the breeding qualities of the sires. In principle, to assess the breeding qualities of producers by the quality of offspring it is possible to use any of the models under consideration, since the established differences for all analyzed characteristics of milk productivity are insignificant (no more than 6%)


Author(s):  
N. V. Kazarovets ◽  
T. V. Pavlova ◽  
K. A. Moiseev

 Over the past decades, in many countries with developed dairy cattle breeding milk yield per cow per lactation has significantly increased, and period of cow productive use has been significantly reduced. The average duration of cow use in many countries is 2.5–2.8 lactations, and this is not enough for sustainable implementation of genetic potential of animals, which is revealed only at the 5–6th lactations. There is an increase in milk productivity and improvement of exterior type of black-and-white breed of cows in the Republic of Belarus, which is due to the use of Holstein cattle with outstanding milk productivity potential and a well-defined milk exterior type, however, as in other countries, there is a decrease in cow use period. The paper presents the results of monitoring of production use of cows in dairy herd conditions with highly productive breeding livestock. 5670 Holsteinized cows of Belarusian black-and-white breed were evaluated in two herds. The main causes of cows culling are determined, effect of genetic (blood level of Holstein breed, level of fathers and mothers performance) and paratypic (age of the 1st calving, level of heifers milking, inter-calving period, service period, results of the first insemination) factors for the life-long milk performance and cow use period. Correlation and heritability of indicators of productive longevity of cows is determined. A system of arrangement and breeding measures to improve longevity of highly productive livestock has been developed. Implementation of the developed system will allow to improve the integrated indices of breeding value of bulls and dairy cows and increase profitability of milk production by increasing the period of use of dairy herd maternal livestock.  Acknowledgments. The work was carried out as part of the state research and technical program “Agropromcomplex – sustainable development” for 2011–2015 sub-task 3.3.1 on topic “Develop system of breeding work with maternal livestock of Holsteinized dairy herds of different productivity levels”. 


REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 109-114
Author(s):  
K.Zh. Zhumanov ◽  
◽  
A.D. Baimukanov ◽  

The article deals with the productive parameters of cows of dairy cattle of the Holstein black-and-white breed in Kazakhstan. The research aimed to determine the level of productivity of the first-calf heifers of the Holstein black-and-white cattle. As an object of the research, information was used on first-calf heifers lactating in 2016-2017 in the breeding herds of the Holstein black-and-white cattle of the Republic of Kazakhstan. The source was the official data of the information-analytical system (IAS) of the livestock breeding of the Republic. The data analysis on the dairy productivity of the cows-daughters of the estimated servicing bulls is carried out according to the indicators of milk yield, the fat and protein content in milk, the yield of milk fat and protein for 305 days of lactation and the research period.


Author(s):  
I. F. Gridyushko ◽  
A. A. Balnikov ◽  
I. P. Sheyko ◽  
O. Y. Vasilyuk ◽  
E. S. Gridyushko

The methods of molecular genetic analysis are widely used to assess the genetic structure and to study the dynamics of population-genetic processes in populations of domestic animals. I n particular, these are the highly polymorphic genetic markers – the microsatellites that are the most common molecular markers in genetic and genomic research. The aim of the research was to develop the genealogical structure of the Belarusian black-and-white breed based on the microsatellite analysis of the lines bred at a gene pool enterprise. The research was carried out with the animals bred at the JSC “SGC “Zarechye”. For the microsatellite analysis of boars of the Belarusian black-and-white breed, the DNA testing was carried out at 9 STR-loci (SО155, SО355, SО386, SО005, SW72, SW951, SО101, SW240, and SW857). In all studied lines, the largest number of effective alleles was at the SО005 locus – 4.00–5.14. The lines of animals with heterozygosity can be used in various breeding schemes, both in purebred breeding (in pedigree pig breeding) and in crossing (in industrial pig breeding). Polymorphism determines the degree of genetic variation in populations. The locus is classified as polymorphic when the frequency of the most common allele of this locus does not exceed 95% (р ≤ 95). The revealed polymorphism of loci in the lines Maket, Tik, and Veseliy indicates the presence of genetic variability in them, which allows using them in breeding and breed-forming processes. Based on the studies made, the genealogical structure of the Belarusian black-and-white breed, consisting of one subcluster and two branches, has been developed, which makes it possible to assess the state and degree of relation of structural units (lines), and reflects the prospect of their further use.


Author(s):  
KH.KH. TAGIROV ◽  
I.V. MIRONOVA ◽  
E.V. POZDNYAKOVA ◽  
L.A. ZUBAIROVA ◽  
N.V. GIZATOVA

В статье приводятся показатели, характеризующие рост и уровень мясной продуктивности бычков черно-пестрой породы при скармливании им люцернового сенажа, заготовленного с разной дозировкой биозакваски на основе пропионовокислых бактерий. Препарат вводили в процессе закладки сенажа в виде рабочего раствора в количестве 2, 4 и 6 л на 1 т консервируемой массы. Опыт проводился в условиях Республики Башкортостан. Для этого 40 10-месячных бычков разделили на 4 группы. Молодняк контрольной группы получал неконсервированный сенаж, а опытных групп — сенаж, с закваской на основе пропионовокислых бактерий в указанных выше дозах. Установлено, что молодняк опытных групп лучше рос и развивался. В 12 мес его живая масса была выше, чем в контрольной группе на 2,50—6,10 кг (0,73—1,77%); в 15 мес — на 9,34—15,14 кг (2,20—3,57%; Р<0,05); в 18 мес — на 15,51—23,31 кг (2,10—4,66%; Р<0,05—0,01). Более высокая энергия роста молодняка опытных групп при откорме на рационах, включающих консервированный сенаж, способствовала и более высоким убойным показателям. Наилучший эффект проявился у бычков, потребляющих сенаж с закваской на основе пропионовокислых бактерий в дозе 4 л рабочего раствора на 1 т зеленой массы. Данная дозировка обеспечивает увеличение массы парной туши на 12,9 кг (4,79%),The article presents the indicators characterizing the growth and level of meat productivity of bull-calves of the black-and-white breed, when they are fed alfalfa haylage, harvested with different dosages of biosakvaski based on propionic acid bacteria. The drug was administered in the process of laying haylage in the form of a working solution in the amount of 2, 4 and 6 liters per 1 ton of preserved mass. The experiment was conducted in the conditions of the Republic of Bashkortostan (Alga, collective farm, Chekmagushevsky district). For this 10-month gobies were divided into 4 groups. The young of the control group did not receive canned haylage, but the test groups received haylage, with sour based on propionic acid bacteria in the above doses. It was found that the young of the experimental groups grew and developed better. So at 12 months of age, their live weight was higher than in the control by 2.50—6.10 kg (0.73—1,77%); in 15 months — by 9,34—15,14 kg (2,20—3,57%; P<0,05); in 18 months — by 15,51—23,31 kg (2,10—4,66%; P<0,05—0,01). The higher growth energy of the young of the experimental groups during fattening on rations, including canned haylage, contributed to a higher slaughter rate. The best effect was manifested in calves consuming haylage with ferment based on propionic acid bacteria in a dose of 4 liters of working solution per 1 ton of green mass.This dosage provides an increase in the mass of steam carcass by 12,9 kg (4,79%), carcass output by 1,0%; slaughter weight — by 14,0 kg (4,95%; P<0,05); slaughter yield — by 1,2%.


Author(s):  
Шайдуллин ◽  
Radik Shaydullin ◽  
Шарафутдинов ◽  
Gazimzyan Sharafutdinov ◽  
Сибагатуллин ◽  
...  

Thanks to DNA diagnostics method it has become possible to identify the genotypes of marker genes of servicing bulls, that makes effective use of manufacturers with the desired gene alleles of economically useful features and is now the main task of breeding in cattle. The analysis and synthesis of polymorphism according to DNA markers of productivity: the kappa-casein (CSN3), diacylglycerol O-acyltransferase (DGAT1), beta-lactoglobulin (LGB), prolactin (PRL), growth hormone (GH), thyroglobulin (TG5), leptin ( LEP) in bulls of Tatarstan type of Kholmogorsk breeding, black-and-white and Kholstein breeding, belonging to tribal enterprises of Tatarstan: JSC Parent enterprise “Elita”, JSC “Menzelinsky breeding enterprise”, JSC “Bugulminsk breeding enterprise”. It was found that the bulls of Tatarstan type of Kholmogorsk breeds have the highest frequency of heterozygous genotypes for most genes, so according to CSN3 genotype AB - 34.2%, according to PRL genotype AB - 30.4%, to TG5 genotype TC - 35.9%, to LEP genotype TC - 66.7% and homozygous genotypes for the genes DGAT1, LGB, PRL, GH, LEP, respectively, AA genotype (47.2%), BB (48.7%), BB (2.2%), VV (20,0%), SS (33.3%). Genetic equilibrium is disrupted on gene of diacylglycerol acyltransferase in bulls of Tatarstan type of Kholmogory breed (χ2 = 4,72; P <0.05) and a black-and-white breed (χ2 = 3,91; P <0.05), and according to the somatropin gene only Kholmogory breed producers (χ2 = 5,42; P <0.05).


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