scholarly journals Growth and Survival of Kanagawa Positive Vibrio parahaemolyticus in Fish and Prawn Preparations Held at Ambient and Elevated Temperature

2001 ◽  
Vol 14 (1) ◽  
Author(s):  
M.N. VENUGOPAL ◽  
I. KARUNASAGAR ◽  
I. KARUNASAGAR ◽  
M.C. VARADARAJ
Keyword(s):  
Elevated Temperature ◽  
Vibrio Parahaemolyticus ◽  
Growth And Survival

scholarly journals Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 280
Author(s):  
Linh Nguyen Thi Truc ◽  
Tuu Nguyen Thanh ◽  
To Tran Thi Hong ◽  
Day Pham Van ◽  
Minh Vo Thi Tuyet ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Vibrio Parahaemolyticus ◽  
Control Group ◽  
Penaeus Vannamei ◽  
Growth And Survival ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Nitrogen Phosphorus ◽  
Shrimp Health

This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.

scholarly journals Modulation of Responses of Vibrio parahaemolyticus O3:K6 to pH and Temperature Stresses by Growth at Different Salt Concentrations

2010 ◽  
Vol 76 (14) ◽  
pp. 4720-4729 ◽  
Author(s):  
W. Brian Whitaker ◽  
Michelle A. Parent ◽  
Lynn M. Naughton ◽  
Gary P. Richards ◽  
Seth L. Blumerman ◽  
...  
Keyword(s):  
Stress Response ◽  
Vibrio Parahaemolyticus ◽  
Acid Stress ◽  
The United States ◽  
Stress Conditions ◽  
Moderate Halophile ◽  
Growth And Survival ◽  
Absolute Requirement ◽  
Acid Acid ◽  
Bacterial Gastroenteritis

ABSTRACT Vibrio parahaemolyticus inhabits marine, brackish, and estuarine waters worldwide, where fluctuations in salinity pose a constant challenge to the osmotic stress response of the organism. Vibrio parahaemolyticus is a moderate halophile, having an absolute requirement for salt for survival, and is capable of growth at 1 to 9% NaCl. It is the leading cause of seafood-related bacterial gastroenteritis in the United States and much of Asia. We determined whether growth in differing NaCl concentrations alters the susceptibility of V. parahaemolyticus O3:K6 to other environmental stresses. Vibrio parahaemolyticus was grown at a 1% or 3% NaCl concentration, and the growth and survival of the organism were examined under acid or temperature stress conditions. Growth of V. parahaemolyticus in 3% NaCl versus that in 1% NaCl increased survival under both inorganic (HCl) and organic (acetic acid) acid conditions. In addition, at 42°C and −20°C, 1% NaCl had a detrimental effect on growth. The expression of lysine decarboxylase (encoded by cadA), the organism's main acid stress response system, was induced by both NaCl and acid conditions. To begin to address the mechanism of regulation of the stress response, we constructed a knockout mutation in rpoS, which encodes the alternative stress sigma factor, and in toxRS, a two-component regulator common to many Vibrio species. Both mutant strains had significantly reduced survival under acid stress conditions. The effect of V. parahaemolyticus growth in 1% or 3% NaCl was examined using a cytotoxicity assay, and we found that V. parahaemolyticus grown in 1% NaCl was significantly more toxic than that grown in 3% NaCl.

Elevated temperature and CO2 have positive effects on the growth and survival of larval Australasian snapper

2020 ◽  
Vol 161 ◽  
pp. 105054
Author(s):  
Shannon J. McMahon ◽  
Darren M. Parsons ◽  
Jennifer M. Donelson ◽  
Steve M.J. Pether ◽  
Philip L. Munday
Keyword(s):  
Elevated Temperature ◽  
Growth And Survival ◽  
Positive Effects

scholarly journals Combined effects of ocean acidification and elevated temperature on feeding, growth, and physiological processes of Antarctic krill Euphausia superba

2021 ◽  
Vol 665 ◽  
pp. 1-18
Author(s):  
GK Saba ◽  
AB Bockus ◽  
CT Shaw ◽  
BA Seibel
Keyword(s):  
Elevated Temperature ◽  
Metabolic Rate ◽  
Ambient Temperature ◽  
Antarctic Krill ◽  
Ph Effect ◽  
Euphausia Superba ◽  
Low Ph ◽  
Interactive Effects ◽  
Growth And Survival ◽  
Key Species

Antarctic krill Euphausia superba is a key species in the Southern Ocean, where its habitat is projected to undergo continued warming and increases in pCO2. Experiments during 2 summer field seasons at Palmer Station, Antarctica, investigated the independent and interactive effects of elevated temperature and pCO2 (decreased pH) on feeding, growth, acid-base physiology, metabolic rate, and survival of adult Antarctic krill. Ingestion and clearance rates of chlorophyll were depressed under low pH (7.7) compared to ambient pH (8.1) after a 48 h acclimation period, but this difference disappeared after a 21 d acclimation. Growth rates were negligible and frequently negative, but were significantly more negative at high (3°C, -0.03 mm d-1) compared to ambient temperature (0°C, -0.01 mm d-1) with no effect of pH. Modest elevations in tissue total CO2 and tissue pH were apparent at low pH but were short-lived. Metabolic rate increased with temperature but was suppressed at low pH in smaller but not larger krill. Although effects of elevated temperature and/or decreased pH were mostly sublethal, mortality was higher at high temperature/low pH (58%) compared to ambient temperature/pH or ambient temperature/low pH (>90%). This study identified 3 dominant patterns: (1) shorter-term effects were primarily pH-dependent; (2) krill compensated for lower pH relatively quickly; and (3) longer-term effects on krill growth and survival were strongly driven by temperature with little to no pH effect.

Growth and Survival of Vibrio parahaemolyticus in Postharvest American Oysters

2002 ◽  
Vol 65 (6) ◽  
pp. 970-974 ◽  
Author(s):  
J. A. GOOCH ◽  
A. DePAOLA ◽  
J. BOWERS ◽  
D. L. MARSHALL
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Geometric Mean ◽  
Fold Increase ◽  
Mobile Bay ◽  
Postharvest Storage ◽  
Growth And Survival ◽  
Naturally Occurring ◽  
Hemolysin Gene ◽  
Plating Method ◽  
Species Specific

Oysters at the retail stage of distribution generally contain greater densities of Vibrio parahaemolyticus than do oysters at harvest. The objective of this study was to determine the effects of postharvest storage at 26 and 3°C on the growth and survival of naturally occurring V. parahaemolyticus in shellstock American oysters (Crassostrea virginica). Oysters were collected monthly from May 1998 through April 1999 from Mobile Bay, Alabama, and their V. parahaemolyticus densities were determined after 0, 5, 10, and 24 h of postharvest storage at 26°C. After 24 h of storage at 26°C, oysters were transferred to a refrigerator at 3°C and analyzed 14 to 17 days later. V. parahaemolyticus numbers were determined by a direct plating method involving an alkaline-phosphatase-labeled DNA probe that targets the species-specific thermolabile hemolysin gene (tlh-AP) to identify suspect isolates. From April to December, when water temperatures at harvest were &gt;20°C, the geometric mean harvest density of V. parahaemolyticus was 130 CFU/g. When water temperatures were &lt;20°C, the geometric mean harvest density was 15 CFU/g. After harvest, V. parahaemolyticus multiplied rapidly in live oysters held at 26°C, showing a 50-fold increase (1.7 log CFU/g) at 10 h and a 790-fold increase (2.9 log CFU/g) at 24 h (April through December). Average V. parahaemolyticus numbers showed a sixfold decrease (0.8 log CFU/g) after approximately 14 days of refrigeration. These results indicate that V. parahaemolyticus can grow rapidly in unrefrigerated oysters.

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