Effectiveness of space spraying in combating Aedes aegypti populations in dengue-endemic areas

2021 ◽  
Vol Volume 55 issue 2 ◽  
Keyword(s):  
Aedes Aegypti ◽  
Endemic Areas

scholarly journals Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

2018 ◽  
Vol 19 (1) ◽  
pp. 31-36 ◽  
Author(s):  
HASMIWATI HASMIWATI ◽  
SELFI RENITA RUSJDI ◽  
EKA NOFITA
Keyword(s):  
Aedes Aegypti ◽  
Novel Mutation ◽  
Hemorrhagic Fever ◽  
Gene Encoding ◽  
Development Of Resistance ◽  
West Sumatra ◽  
Primary Vector ◽  
Endemic Areas ◽  
Larval Susceptibility

Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especially in Padang City. Synthetic insecticide control is one currently used method to prevent mosquito-borne diseases. The extensive, long-term application of Temephos along with inappropriate dosages, have resulted in the development of resistance in Ae. aegypti populations. Mutation of the Ace-1 gene, encoding an acetyl cholinesterase, is one of the mechanisms that confer resistance to organophosphate (OP). The Temephos resistance status of Ae. aegypti in Padang city has not yet been studied. This study aimed to investigate the resistance status of Ae. aegypti and identify any possible mutation (s) of the Ace-1 gene in Padang city. Ae. aegypti samples were collected in five population in Padang city (Jati (JT), Gunung Pangilun (GP), Lubuk Minturun (LM), Korong Gadang (KG), and Bandar Buat (BB)). The larval susceptibility to Temephos was tested by larval bioassays with Temephos pestanal at 0.02 mg/L dosages. Larval susceptibility was determined by mortality percentage values. The relationship between Ace-1 genotypes and the resistant phenotype was analyzed by percentage of genotype frequency. Out of five populations, assessed by larval bioassays, JT and GP were resistant to Temephos; LM, KG, and BB were tolerant. A total of 50 individuals from larval bioassays were genotyped for Ace-1 gene. Our findings showed that Ace-1 was 495 bp in length. Mutation was not found in the G119S location but in the T506T location. Three alleles in T506T location were detected, including a wild type allele, TT (65.21%), and two mutant alleles, TA (26.08%), AA (8.69%). The use of Temephos showed that some Ae. aegypti populations were resistant, others were tolerant, but no population was vulnerable to Temephos. A novel mutation was detected as substitution in T506T location (ACT>ACA).

scholarly journals The Susceptibility of Aedes aegypti In Dengue Endemic Areas, Tegal, Central Java Indonesia

2021 ◽  
pp. 11-18
Author(s):  
Irfan Kresnadi ◽  
Bany Faris Amin ◽  
Haekal Ariq ◽  
Viharsyah Aulia Akbar ◽  
Rawina Winita ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Endemic Area ◽  
Control Program ◽  
Central Java ◽  
Endemic Areas

Tegal district is a dengue-endemic area. One of the strategies to control Ae. aegypti is the use of insecticides. The determination of insecticide resistance in a dengue-endemic area is useful for supporting policies for Ae. aegypti control program. The aim of this study is to determine the susceptibility of Ae. aegypti in Tegal district, Central Java. Aedes aegypti larvae were collected from June to July 2018. Susceptibility bioassay of Ae. aegypti larvae against temephos and Ae. aegypti female against permethrin were conducted refers to the WHO protocol. The susceptibility of Ae. aegypti was interpreted based on WHO protocol as well. The mortality of Ae. aegypti larvae were at 0.025 ppm by 90%. The LC50 at 0.0005 ppm, and LC99 at 1.1037 ppm, respectively. The mortality rate of Ae. aegypti against permethrin was 26%. The LT50 at 6611.636 minutes, and LT99 at 5958807.272 minutes, respectively. The susceptibility of Ae. aegypti larvae were possible resistant but adult Ae. aegypti was resistant.

scholarly journals Ovitrap Surveillance of Aedes aegypti and Aedes albopictus in Dengue Endemic Areas in Keramat and Shah Alam, Selangor in 2016

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Noor Afizah A ◽  
Mohd Arif AK ◽  
Nazni WA ◽  
Lee HL
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Species Level ◽  
Dengue Vector ◽  
Entomological Surveillance ◽  
Minimum Number ◽  
Endemic Areas ◽  
The Difference ◽  
Study Sites ◽  
Indoor And Outdoor

Introduction: Entomological surveillance is crucial to determine the abundance of dengue vector and to evaluate breeding areas of Aedes aegypti and Aedes albopictus. The objective of this study is to determine the distribution and breeding preference for both Ae. aegypti and Ae. albopictus in dengue endemic areas. Materials and Methods: Ovitraps surveillance was conducted in two dengue endemic areas; AU2, Keramat and Seksyen 7, Shah Alam, Selangor. A minimum number of 100 ovitraps were deployed for 5 days in the study sites. Samples collected were brought back to the lab and all larvae recovered were identified to species level. Results: The ovitap index (OI) in both localities exceeded the transmission threshold of 10% with the OI recorded ranged from 42.3-79.8% in AU2, Keramat and 16.7-42.9% in Seksyen 7, Shah Alam. Ae. albopictus was the dominant species in AU2 Keramat with the highest ratio Ae. aegypti to Ae. albopictus recorded was 1.00:22.79. Nonetheless, in Seksyen 7, Shah Alam the difference in Ae. aegypti to Ae. albopictus ratio is not really prominent with 1.00:3.61 for ovitraps deployed outdoor and 3.40:1.00 for ovitraps set indoor. It was determined that single infestation of either Ae. aegypti or Ae. albopictus is more frequent for ovitraps deployed indoor and/or outdoor, respectively. It was also determined that mixed infestations were found in this study indicating that both species can oviposit in the same container. Conclusion: This study indicates that OI is still above transmission threshold in both study sites. While Ae. aegypti and Ae. albopictus remain as a dominant indoor and outdoor breeder, respectively, mixed breeding of Aedes species in a same container was also observed.

scholarly journals PERBEDAAN STATUS KERENTANAN NYAMUK AEDES AEGYPTI TERHADAP MALATHION DI KABUPATEN BANTUL YOGYAKARTA

2016 ◽  
Vol 11 (2) ◽  
pp. 302
Author(s):  
Liena Sofiana ◽  
Muhammad Surya Rahman
Keyword(s):  
Aedes Aegypti ◽  
Laboratory Test ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
The Status ◽  
Endemic Areas ◽  
Different Levels

<p><strong>Abstrak</strong></p><p>Demam berdarah dengue di Kabupaten Bantul selama 5 tahun terakhir menunjukkan wilayah Kecamatan Sewon termasuk dalam wilayah endemis dan Kecamatan Dlingo merupakan Kecamatan dan desa yang memiliki angka kejadian yang paling rendah, bahkan di Desa Dlingo pernah tidak mengalami kejadian Demam berdarah dengue. Penggunaan malathion sebagai salah satu upaya pencegahan yang berlangsung lama di Desa Panggungharjo dimungkinkan dapat terjadi resistensi, sehingga tujuan penelitian ini adalah untuk mengetahui status resistensi malathion di Desa Panggungharjo dan Dlingo. Penelitian pada tahun 2014 merupakan penelitian deskriptif dengan uji laboratorium. Objek penelitian dalam penelitian ini adalah nyamuk Aedes aegypti hasil rearing yang diperoleh di Desa Panggungharjo dan Dlingo Bantul yang kemudian diuji secara suseptibiltas. Hasil penelitian menunjukkan bahwa nyamuk Aedes aegypti yang berasal dari Desa Panggungharjo telah resistan dan nyamuk Aedes aegypti yang berasal dari Desa Dlingo tidak resisten melainkan masih dalam kategori sedang. Ada perbedaan tingkat resistensi malathion antara Desa Panggungharjo dan Dlingo Bantul Yogyakarta.</p><p>Kata Kunci : Aedes aegypti; Malation; Kerentanan; Resistensi.</p><p> </p><p><strong><em>Abstract</em></strong></p><p><em>Dengue hemorrhagic fever in the district Bantul during the last 5 years shows Sewon district area is also included in the endemic areas and Dlingo is a village that has the lowest incidence. Even at some time in Dlingo there was an  occurrence of dengue hemorrhagic fever. Prolonged use of malathion as one prevention in the Panggungharjo district  may can occur in resistance with this dengue. Purpose of this study was to determine the status of malathion resistance in the Panggungharjo and Dlingo. The research in 2014  was descriptive with laboratory test.  Research object is the Aedes aegypti rearing results obtained from the Panggungharjo and Dlingo Bantul and then tested in susceptibility. The results of this study are Aedes aegypti from the Panggungharjo was resistant with and Aedes aegypti from Dlingo is not resistant but still in the tolerant category. There have different levels of resistance between Panggungharjo and Dlingo Bantul Yogyakarta.</em></p><p><em>Keywords : Aedes aegypti; Malathion; Susceptibility; Resistance.</em></p>

scholarly journals The Differences Analysis of Temperature, Salinity, and Dissolved Oxygen of Aedes aegypti Breeding Place Water in Endemic and Non-Endemic Areas

2019 ◽  
Vol 15 (2) ◽  
pp. 89
Author(s):  
Nurul Hidayah ◽  
Dwi Rahmawati
Keyword(s):  
Water Quality ◽  
Aedes Aegypti ◽  
Dissolved Oxygen ◽  
Statistical Tests ◽  
Control Group ◽  
P Value ◽  
Case Group ◽  
Breeding Sites ◽  
Mean Temperature ◽  
Endemic Areas

Abstract: The study aims to determine differences in water quality of breeding sites in endemic and non-endemic areas. The method used is quantitative observational analytic with a case control study design. Case group was water parameters in endemic areas and the control group is in non-endemic areas. 43 samples of breeding water were taken from each area then water quality measurements were carried out. Data were analyzed using independent t-test. The results obtained mean temperature in endemic areas 27.51 ± 0.739 oC, salinity 2,544 ± 0.638 gr/l, and DO 7,253 ± 1,097 mg/l. The mean temperature in non-endemic areas is 25.7 ± 1.124 oC, salinity is 2.472 ± 2.365 gr/l, and DO is 6.479 ± 1.059 mg/l. P-value of statistical tests of differences in temperature, salinity, and DO parameters in endemic and non-endemic areas are 0.000, 0.266, and 0.001. It was concluded that temperature and DO parameters in endemic areas proved to be significantly different from those in non-endemic areas. However, for salinity variables there are no significant differences. Keywords: Aedes aegypti, dissolved oxygen, salinity, temperature

scholarly journals Bio-physicochemical markers of the Aedes aegypti breeding water in endemic and non-endemic area

2019 ◽  
Vol 8 (2) ◽  
pp. 151
Author(s):  
Nurul Hidayah ◽  
Dwi Rahmawati
Keyword(s):  
Aedes Aegypti ◽  
Endemic Area ◽  
Sampling Technique ◽  
Control Group ◽  
Case Group ◽  
Chi Square ◽  
Breeding Sites ◽  
Endemic Areas ◽  
And Control ◽  
Control Study

The survival of <em>Aedes aegypti</em> larvae is inseparable from the adequacy of food, including organic substances available in the breeding water. It is very dependent on the level of water markers such as temperature, salinity, Dissolved Oxygen, and pH. The study used quantitative observational analytic with a case-control study design. Case group has consisted of breeding water in endemic area and control group was in non-endemic area. The sample size was 43 samples for each group, collected by purposive sampling technique. Data were analyzed by Chi-square and Mann-Whitney test. Larvae mostly presence in endemic area (68.3%) and mostly absent in non-endemic area (85.4%) (<em>p</em>-value = 0.002). Temperature in endemic area mostly in 27-30<sup>o</sup>C (86%) and non-endemic area mostly in &lt;27<sup>o</sup>C or &gt;30<sup>o</sup>C (72.1%) (<em>p</em>-value = 0.000). Salinity in endemic and non-endemic areas has no difference (<em>p</em>-value = 0.266). DO in endemic areas were mostly in 5.02-7.82 mg/l (76.7%). While DO in non-endemic area was mostly in &lt;5.02 mg/l or &gt;7.82 mg/l (95.3%) (<em>p</em>-value = 0.001). The pH &lt;6 or &gt;7.8 is mostly in non-endemic areas (87.8%) and pH 6-7.8 is mostly in endemic areas (63.4%) (<em>p</em>-value = 0.000). Bio-physicochemical markers of breeding sites water have differences between endemic and non-endemic area except salinity. The temperature, salinity, DO, and pH affected the presence of larvae and the most affected is DO marker. While the marker that affected the presence of larvae in the non-endemic area is pH.

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