scholarly journals In vitro study on the antitumor activity of Tanacetum vulgare L. extracts

2019 ◽  
Vol 51 (2) ◽  
pp. 249-255
Author(s):  
A. M. Vasileva ◽  
I. A. Iliev ◽  
V. S. Lozanov ◽  
M. B. Dimitrova ◽  
V. I. Mitev ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Tanacetum Vulgare ◽  
Dicaffeoylquinic Acid ◽  
Flavonoid Aglycone ◽  
Ethyl Acetate Extracts

The major nonvolatile compounds derived from extracts and fractions from Tanacetum vulgare L. flowers were determined by LC–HRMS. Major compounds in the crude extract were determined to be: six hydroxycinnamoyl quinic acids with 4,5-dicaffeoylquinic acid and twelve flavonoids and their derivatives, six of which were in the form of flavonoid-O-glucuronides. Generally, the major flavonoid aglycone in tansy was luteolin. Extracts and fractions were tested under in vitro conditions in nine cell lines - one control non-tumorogenic and eight tumor lines, whereby antitumor activity was observed after 72 hours of incubation with the aforementioned substances as determined by an MTT assay. The obtained results show the highest selectivity index for the ethyl acetate extract from Flores Tanaceti (EAFT) and for the ethyl acetate fraction of the crude extract (EACE). EAFT extract was found to exert the highest antitumor effect, followed by EACE. From the above results it becomes evident that ethyl acetate extracts of T. vulgare contain substances with high selective activity against tumor cells.

scholarly journals Antitumor Activities of Ethyl Acetate Extracts fromSelaginella doederleiniiHieronIn VitroandIn Vivoand Its Possible Mechanism

2015 ◽  
Vol 2015 ◽  
pp. 1-9
Author(s):  
Jia-zhi Wang ◽  
Juan Li ◽  
Ping Zhao ◽  
Wen-tao Ma ◽  
Xie-he Feng ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Ethyl Acetate ◽  
Cell Apoptosis ◽  
Mrna Level ◽  
Pcr Analysis ◽  
Antitumor Activities ◽  
Cox 2 ◽  
Ethyl Acetate Extracts

The antitumor activities of ethyl acetate extracts fromSelaginella doederleiniiHieron (SD extracts)in vitroandin vivoand its possible mechanism were investigated. HPLC method was developed for chemical analysis. SD extracts were submitted to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on different cells, flow cytometry, and RT-PCR analysis using HepG2 cell and antitumor activityin vivousing H-22 xenograft tumor mice. Six biflavonoids from SD extracts were submitted to molecular docking assay. The results showed that SD extracts had considerable antitumor activityin vitroandin vivowithout obvious toxicity on normal cells and could induce cell apoptosis. The mechanisms of tumorigenesis and cell apoptosis induced by SD extracts may be associated with decreasing the ratio of bcl-2 and bax mRNA level, activating caspase-3, suppressing survivin, and decreasing the gene expression of COX-2, 5-LOX, FLAP, and 12-LOX mRNA. The main active component in SD extracts is biflavonoids and some exhibited strong interactions with COX-2, 5-LOX, 12-LOX, and 15-LOX. These results offering evidence of possible mechanisms of SD extracts suppress cell proliferation and promote apoptosis and provide the molecular theoretical basis of clinical application ofS. doederleiniifor cancer therapy.

scholarly journals Gendola Leaf Ethyl Acetate Fraction (Basella rubra Linn) Reduces Spermatozoa Motility and Viability in Vitro

2021 ◽  
Vol 5 (2) ◽  
pp. 228-233
Author(s):  
Lusia Hayati ◽  
Joko Marwoto ◽  
Septi Purnamasari ◽  
Yuni Fitriayanti
Keyword(s):  
Treatment Group ◽  
Ethyl Acetate ◽  
Calculated Data ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Control Group ◽  
Phenol Compounds ◽  
Spermatozoa Motility ◽  
Motility Of Sperm

A B S T R A C TBackground. The use of plants as anti-fertility in men has been done to reduce sideeffects. The Gendola plant is one of the Basellaceae family plants, which is a naturalmedicinal plant in Indonesia. Gendola leaves contain phenol compounds, flavonoids,tannins, steroids and triterpenoids which can have cytotoxic activity. This studyaims to determine the in vitro anti sperm activity of the ethyl acetate fraction ofGendola leaves on male spermatozoa. Methods. This study is an experimentallaboratory in vitro study using male sperm preparations. The sample used was 20men for each group, both the group was given the gondola leaf fraction and thecontrol group. The treatment group was given ethyl acetate fraction of Gendola leaves(Basella rubra Linn.) With a concentration of 0.05% for 5, 10 and 15 minutes, theviability and motility of sperm were calculated. Data analysis was performed usingthe Mann Whitney test (p <0.05) using SPSS release for Windows software. Results.The results showed that the ethyl acetate fraction affected the motility and viabilityof spermatozoa. When compared with the control group, the motility treatment groupobtained was classified as non-progressive. In the treatment group, the viability ofspermatozoa was significantly different at minute 5, minute 10 and minuted 15 (p<0.05). Conclusion. Ethyl acetate fraction has potential as an anti sperm inreducing sperm motility and viability.

scholarly journals In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Zemene Demelash Kifle ◽  
Simachew Gidey Debeb ◽  
Yaschilal Muche Belayneh
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Ethyl Acetate Fraction ◽  
Maximum Activity ◽  
Chloroform Fraction ◽  
Aqueous Fraction ◽  
Water Fraction ◽  
Hagenia Abyssinica

Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. abyssinica leaves. Methods. The in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of α-amylase, α-glucosidase, and DPPH scavenging effect was presented as means ± SEM ( n = 3 ). Results. The α-amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration-dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC50, 14.52 μg/ml) followed by water fraction 68.24% (IC50, 16.31 μg/ml), ethyl acetate fraction 61.57% (IC50, 18.73 μg/ml), and chloroform fraction 56.87% (IC50, 21.57 μg/ml) of H. abyssinica leaves. In the α-glucosidase inhibition assay, the maximum activity was exhibited by the aqueous fraction 62.54% (IC50, 11.67 μg/ml) followed by ethyl acetate fraction 54.97% (IC50, 15.89 μg/ml), crude extract 46.79% (IC50, >16.5 μg/ml), and chloroform fraction 36.44% (IC50, >16.5 μg/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC50, 10.25 μg/ml) followed by water fraction 78.59% (IC50, 13.86 μg/ml), ethyl acetate fraction 71.58% (IC50, 16.34 μg/ml), and chloroform fraction 63.65% (IC50, 18.83 μg/ml). Conclusion. This study has revealed that H. abyssinica leaves possess noticeable in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities.

scholarly journals In Vitro Phytochemistry and Antiplasmodial Activity of Leaf Extract and Fractions of Nauclea diderrichii

2019 ◽  
pp. 333-342
Author(s):  
M. Abdulrahman ◽  
M. Adamu
Keyword(s):  
Plasmodium Falciparum ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Leaf Extract ◽  
Folk Medicine ◽  
Ethyl Acetate Fraction ◽  
Antiplasmodial Activity ◽  
Crude Leaf Extract ◽  
Nauclea Diderrichii

The current investigation deals with phytochemical screening and in vitro antiplasmodial activity of crude ethanol leaf extract and three fractions of crude ethanol leaf-extract of Nauclea diderrichii. Phytochemical test to screen bioactive compounds was carried out via standard protocols which uncovered the presence of alkaloids, saponins, steroids, phenols, tannins, flavonoids, glycosides and carbohydrates, extraction was done using absolute ethanol to afford the crude extract (Nd-ET) while maceration was done using solvents of different polarity gradient (petroleum ether, chloroform and ethyl acetate) to afford the remaining fractions (Nd-F1, Nd-F2 and Nd-F3). The antiplasmodial activity of the crude-extract and those of crude-extract-fractions against plasmodium falciparum unveil promising percentage elimination at all concentrations, with ethanol crude extract (Nd-ET) and ethyl acetate fraction (Nd-F3) having the highest, with 75.50% and 72.65% at 625µg/ml, and 87.83% and 86.33% at 5000µg/ml, respectively. These results clearly indicated that the active compounds present in the crude leaf extract/fractions of Nauclea diderrichii are highly potent eliminators of plasmodium falciparum and validate their popular usage in folk medicine in Gusau Local Government, Zamfara State, Nigeria, for the treatment of malaria.

scholarly journals In Vitro Antidiabetic Activities of Extract and Isolated Flavonoid Compounds from Artocarpus altilis (Parkinson) Fosberg

2014 ◽  
Vol 14 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Puspa Dewi Narrij Lotulung ◽  
Tjandrawati Mozef ◽  
Chandra Risdian ◽  
Akhmad Darmawan
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Inhibition Mechanism ◽  
Artocarpus Altilis ◽  
Flavonoid Compounds ◽  
Mechanism Of Inhibition ◽  
Ethyl Acetate Extracts

The antidiabetic activity test through a mechanism of inhibition of α-glucosidase enzyme was studied against ethanol, n-hexane, ethyl acetate and n-butanol fractions of ethanol extract of Artocarpus altilis (Parkinson) Fosberg (Moraceae) leaves and four flavonoid compounds isolated from ethyl acetate extracts of A. altilis. Ethyl acetate fraction has strongest antidiabetic activity compared to ethanol, n-hexane, and n-butanol fractions with IC50values5.98,6.79, 440.18and14.42μg/mL, respectively. Four flavonoid compounds (1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl]-1-propanone (AC-31), 2-geranyl-2',3,4,4'-tetrahydroxy dihydrochalcone (AC-51), 8-geranyl-4',5,7-trihydroxyflavone (AC-33) andcyclocommunol (AA-3), have been isolated from ethylacetate fraction. AC-31 was the strongest antidiabetic compound compared to AC-51, AC-33 and cyclocommunolwithIC50values are 15.73, 24.41,49.49,and72.20μg/mL. Kineticstudies of AC-31 using Lineweaver-Burk method showed that inhibition mechanism of enzymeα-glucosidase was anon-competitivetype.

scholarly journals Potensi Kulit Batang Bungur (Lagerstroemia loudonii Teijsm and Binn.) Sebagai Herbal Antidiabetes dengan Mekanisme Penghambat Alfa-glukosidase

2018 ◽  
Vol 1 (3) ◽  
pp. 117-120
Author(s):  
Soraya Riyanti ◽  
Julia Ratnawati ◽  
Muhammad Ibnu Shaleh ◽  
Asep Gana Suganda
Keyword(s):  
Ethyl Acetate ◽  
Colorimetric Method ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Water Fraction ◽  
Ic50 Value ◽  
The Family ◽  
Ic50 Values ◽  
Alpha Glucosidase

Tumbuhan bungur (Lagerstroemia loudonii T.B.) termasuk dalam famili Lytrharceae. Famili Lythraceae telah diketahui memiliki aktivitas farmakologi sebagai antidiabetes, antiinflamasi, antimikroba, serta antiobesitas. Daun dan buah bungur (Lagerstroemia loudonii T.B.) memiliki aktivitas dalam menghambat alfa-glukosidase. Berdasarkan teori khemotaksonomi didalam tumbuhan, kemungkinan bagian lain dari tumbuhan bungur memiliki aktivitas dan kandungan kimia yang sama, sehingga dilakukan pengujian aktivitas penghambatan terhadap alfa-glukosidase pada bagian kulit batang bungur. Ekstraksi simplisia dilakukan dengan cara maserasi selama 24 jam menggunakan pelarut etanol 96%. Proses fraksinasi menggunakan cara Ekstraksi Cair-Cair (ECC) dengan pelarut n-heksana, etil asetat dan air. Pengujian aktivitas penghambatan alfa-glukosidase secara in vitro menggunakan metode kolorimetri dengan alat spektrofotometer UV-VIS pada panjang gelombang 400,4 nm dengan substrat p-nitrofenil-α-D-glukopiranosid (PNPG). Akarbose digunakan sebagai pembanding. Hasil penelitian menunjukkan bahwa ekstrak, fraksi air, fraksi etil asetat dan fraksi n-heksana memiliki nilai IC50 berturut-turut sebesar 240,53±0,47 μg/ml, 186,111±1,02 μg/ml, 79,479±0,52 μg/ml dan 113,101±0,46 μg/ml. Nilai IC50 akarbose adalah sebesar 10,457±1,48 μg/ml. Ekstrak dan fraksi-fraksi (air,etil asetat dan n-heksana) kulit batang bungur mampu menghambat aktivitas enzim α-glukosidase. Aktivitas yang paling baik ditunjukan oleh fraksi etil asetat dengan nilai IC50 sebesar 79,479±0,52 μg/ml.   Bungur (Lagerstroemia loudonii T.B.) is included in the Family  Lytrharceae. The Lythraceae has been known to have pharmacological activity as antidiabetic, anti-inflammatory, antimicrobial, and antiobesity. Leaves and fruits of bungur (Lagerstroemia loudonii T.B.) have activities to inhibit alpha-glucosidase. Based on the chemotaxonomy theory in plants, it is possible that other parts of the bungur plant have the same chemical activity and content, Hence the study to evalute the  inhibitory activity against alpha-glucosidase was carried out on its bark stem.The extraction of dried powder material was carried out by maceration for 24 hours using 96% ethanol. The extract was fractionated by Liquid-Liquid Extraction (ECC) method with n-hexane, ethyl acetate and water. The In vitro study of alpha-glucosidase inhibition activity using a colorimetric method with a UV-VIS spectrophotometer at a wavelength of 400.4 nm with a p-nitrophenyl-α-D-glucopiranoside (PNPG) substrate was performed. Akarbose was used as a standard drug.The results showed that extract, water fraction, ethyl acetate fraction and n-hexane fraction showed IC50 values ​​of 240.53 ± 0.47 μg / ml, 186.111 ± 1.02 μg / ml, 79.497 ± 0.52 μg/ ml and 113.101 ± 0.46 μg / ml, respectively. The IC50value of bungur was 10.457 ± 1.48 μg / ml. Extracts and fractions (water, ethyl acetate and n-hexane) of bark stem were able to inhibit the activity of α-glucosidase. Theethyl acetate fraction showed the strongestactivity with IC50 value of 79,479 ± 0.52 μg / ml

scholarly journals Gendola Leaf Ethyl Acetate Fraction (Basella rubra Linn) Reduces Spermatozoa Motility and Viability in Vitro

2021 ◽  
Vol 5 (3) ◽  
pp. 272-277
Author(s):  
Lusia Hayati ◽  
Joko Marwoto ◽  
Septi Purnamasari ◽  
Yuni Fitriayanti
Keyword(s):  
Treatment Group ◽  
Ethyl Acetate ◽  
Calculated Data ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Control Group ◽  
Phenol Compounds ◽  
Spermatozoa Motility ◽  
Motility Of Sperm

A B S T R A C TBackground. The use of plants as anti-fertility in men has been done to reduce sideeffects. The Gendola plant is one of the Basellaceae family plants, which is a naturalmedicinal plant in Indonesia. Gendola leaves contain phenol compounds, flavonoids,tannins, steroids and triterpenoids which can have cytotoxic activity. This studyaims to determine the in vitro anti sperm activity of the ethyl acetate fraction ofGendola leaves on male spermatozoa. Methods. This study is an experimentallaboratory in vitro study using male sperm preparations. The sample used was 20men for each group, both the group was given the gondola leaf fraction and thecontrol group. The treatment group was given ethyl acetate fraction of Gendola leaves(Basella rubra Linn.) With a concentration of 0.05% for 5, 10 and 15 minutes, theviability and motility of sperm were calculated. Data analysis was performed usingthe Mann Whitney test (p <0.05) using SPSS release for Windows software. Results.The results showed that the ethyl acetate fraction affected the motility and viabilityof spermatozoa. When compared with the control group, the motility treatment groupobtained was classified as non-progressive. In the treatment group, the viability ofspermatozoa was significantly different at minute 5, minute 10 and minuted 15 (p<0.05). Conclusion. Ethyl acetate fraction has potential as an anti sperm inreducing sperm motility and viability.

scholarly journals CYTOTOXICITY ACTIVITY OF PICRIA FEL-TERRAE LOUR. HERBS AGAINST 4T1 AND MCF-7 BREAST CANCER CELLS

2018 ◽  
Vol 11 (13) ◽  
pp. 194
Author(s):  
Urip Harahap ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Panal Sitorus ◽  
Denny Satria
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Breast Cancer Cells ◽  
In Vitro Study ◽  
Ethyl Acetate Fraction ◽  
Cytotoxicity Activity ◽  
Mtt Method ◽  
Mcf 7

 Objective: This study was carried out to investigate the cytotoxic activity toward 4T1 and MCF-7 cell lines of Picria fel-terrae Lour. herb fractions.Methods: P. fel-terrae Lour. herb powder was extracted by maceration method with n-hexane, ethyl acetate, and ethanol solvent. In vitro study was using MTT method toward 4T1 and MCF-7 cell lines.Results: The inhibitory concentration 50% was 234.10 ± 7.85, 50.49 ± 1.07, and 212.53 ± 7.55 μg/mL for 4T1 and 84.62 ± 1.44, 56.79 ± 0.22, and 235.51 ± 4.77 μg/mL for MCF-7 cell lines, respectively.Conclusion: The results reveal that P. fel-terrae Lour. herb fractions provide effective as anticancer. Our further study is to assess the mechanism of ethyl acetate fraction in inhibit angiogenesis and metastatic in breast cancer.

scholarly journals Ethyl Acetate Fraction of Helianthus tuberosus L. Induces Anti-Diabetic, and Wound-Healing Activities in Insulin-Resistant Human Liver Cancer and Mouse Fibroblast Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 99
Author(s):  
Arokia Vijaya Anand Mariadoss ◽  
SeonJu Park ◽  
Kandasamy Saravanakumar ◽  
Anbazhagan Sathiyaseelan ◽  
Myeong-Hyeon Wang
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Scavenging Activity ◽  
Helianthus Tuberosus ◽  
Insulin Resistant ◽  
Dicaffeoylquinic Acid

Traditional, complementary, and integrative medicine are globally accepted alternative methods for the treatment of diabetes mellitus (DM). However, the mechanism of anti-diabetic effects of Helianthus tuberosus L. remains unproven. In the present study, antioxidant and anti-diabetic activity of the tubers of H. tuberosus were studied in detail. Methanolic extracts of H. tuberosus tubers were subjected to solvent fractionation method by increasing the polarity of the solvent using n-hexane, and ethyl acetate. The obtained methanol extracts and its fractions were subjected to free radical scavenging activity (DPPH and ABTS assay) and in vitro enzyme (α-amylase and α-glucosidase) inhibition assay. Moreover, glucose uptake in insulin-resistant HepG2 cell line was analyzed. The preliminary phytochemical analysis confirmed the presence of phenolic and flavonoid compounds in the active fraction. The radical scavenging and in vitro diabetic related enzyme inhibitory activities were found to be dose dependent. The maximum ABTS+ and DPPH scavenging activity was documented in ethyl acetate fraction of the H. tuberosus followed by methanol extract, hexane fraction, and methanol fraction. We also found that H. tuberosus showed a less toxicity in mouse fibroblast cells and enhance the glucose uptake in insulin-resistant HepG2 cells. Besides, the ethyl acetate fraction of the H. tuberosus analyzed by UPLC-QTOF-MS-MS and GC/MS revealed the presence of phenolic compounds such as neochlorogenic acid, chlorogenic acid, caffeic acid, 5-O-(4-coumaroyl)-quinic acid, feruloylquinic acid, caffeoylquinic acid, isoxazolidine, salicylic acid β-D-glucoside, dicaffeoylquinic acid isomers, salvianolic acid derivative isomers, and 1,4 dicaffeoylquinic acid etc. Among the identified phytochemicals, six were chosen for molecular docking study to explore their its inhibitory interactions with α-amylase and α-glucosidase. Taken together, the findings of the present study suggested that phytocompounds of EAF were responsible for the significant in vitro antioxidant, wound-healing, and anti-diabetic activities.

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