Faculty Opinions recommendation of Exploring the "hair growth-wound healing connection": anagen phase promotes wound re-epithelialization.

Deer antlers are the only mammalian appendage capable of regeneration. We aimed to investigate the effect of red deer antler extract in regulating hair growth, using a mouse model. The backs of male mice were shaved at eight weeks of age. Crude aqueous extracts of deer antler were prepared at either 4°C or 100°C and injected subcutaneously to two separate groups of mice (n=9) at 1 mL/day for 10 consecutive days, with water as a vehicle control group. The mice skin quantitative hair growth parameters were measured and 5-bromo-2-deoxyuridine was used to identify label-retaining cells. We found that, in both the 4°C and the 100°C deer antler aqueous extract-injection groups, the anagen phase was extended, while the number of BrdU-incorporated cells was dramatically increased. These results indicate that deer antler aqueous extract promotes hair growth by extending the anagen phase and regulating cell proliferation in the hair follicle region.

Download Full-text

The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

Applied Sciences ◽

10.3390/app10082646 ◽

2020 ◽

Vol 10 (8) ◽

pp. 2646

Author(s):

Keng-Liang Ou ◽

Yun-Wen Kuo ◽

Chia-Yu Wu ◽

Bai-Hung Huang ◽

Fang-Tzu Pai ◽

...

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Hair Follicle ◽

Conditioned Medium ◽

Hair Growth ◽

Hair Regeneration ◽

Hacat Keratinocyte ◽

Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

Download Full-text

CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen

Genes ◽

10.3390/genes11030297 ◽

2020 ◽

Vol 11 (3) ◽

pp. 297 ◽

Cited By ~ 2

Author(s):

Yuxin Xu ◽

Hongmei Liu ◽

Huilin Pan ◽

Xinyue Wang ◽

Yuxin Zhang ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Hair Growth ◽

Negative Regulator ◽

Fibroblast Growth ◽

Hair Cycle ◽

Ideal Model ◽

Anagen Phase ◽

Hematoxylin And Eosin ◽

Scanning Electron

Hair growth and morphology are generally regulated by the hair cycle in mammals. Fibroblast Growth Factor 5 (FGF5), which is a hair cycle regulator, has a role in regulating the hair cycle during the transition from the anagen phase to the catagen phase, and a hereditary long hair phenotype has been widely reported when FGF5 is mutated in humans and other species. However, there has been no such report in rabbits. Thus, the first exon of rabbit FGF5 was disrupted by the CRISPR/Cas9 system, and the phenotype of FGF5-/- rabbits was characterized while using hematoxylin and eosin (H&E) staining, immunohistochemistry, quantitative PCR, scanning electron microscopy, and western blotting. The results showed a significant and systemic long hair phenotype in the FGF5-/- rabbits, which indicated that FGF5 is a negative regulator of hair growth. In addition, a decreased diameter of the fiber and a higher area proportion of hair follicle clusters were determined in FGF5-/- rabbits as compared with the WT rabbits. Further investigation verified that prolonging the anagen phase in rabbits, with decreased BMP2/4 pathway signaling and increased VERSICAN pathway signaling, caused the systemic long hair phenotype. Taken together, these results indicate a systemic long hair phenotype by prolonging anagen in FGF5-/- rabbits, which could be widely used for Fur production and an ideal model for studying the mechanism of long hair in the future.

Download Full-text

Molecular Pathways Involved in Promoting Activity of Timosaponin BII on Hair Growth in C57BL/6 Mice

BioMed Research International ◽

10.1155/2020/9451596 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Lei Xiao ◽

Xia Zhang ◽

Zhiyi Chen ◽

Jianhua Li ◽

Bing Li ◽

...

Keyword(s):

Hair Follicle ◽

Hair Growth ◽

Immunohistochemical Analysis ◽

Hair Follicles ◽

Common Disease ◽

Male Mice ◽

High Concentration ◽

Anagen Phase ◽

Approved Drugs ◽

Anemarrhena Asphodeloides

Hair loss is a common disease in dermatology, while the approved drugs may have unpredictable side effects. In this study, the effect of timosaponin BII extracted from Anemarrhena asphodeloides on hair growth of C57BL/6 mice was investigated by measuring the hair follicle morphology, hair growth length and area in C57BL/6 male mice, and the immunohistochemical analysis of β-catenin, Wnt3a, and Wnt10b in the dorsal skins of mice after topical application with minoxidil and timosaponin BII for 15 days. The decrease in skin brightness, the increase in the regrowing area of hair and hair follicles numbers, and the improvement of hair follicle morphology in the group applied with 0.5% timosaponin BII indicated an induction of the anagen phase in telogenic mice skin, which were comparative to the 2% minoxidil treatment. The immunohistochemical analysis detected an increase in the expression of β-catenin and Wnt10b, supporting the theory of the activation of the β-catenin/Wnt pathway was one of the pathways that are related to anagen phase induction. Anemarrhena asphodeloides is a herb commonly used for metabolic disorders in China. The present study is the first to show that the timosaponin BII, which is present at a high concentration in A. asphodeloides, promotes hair growth in C57BL/6 male mice. The results indicate that timosaponin BII may be a potential promoting agent for hair growth.

Download Full-text

Dermal exosomes containing miR-218-5p promote hair regeneration by regulating β-catenin signaling

Science Advances ◽

10.1126/sciadv.aba1685 ◽

2020 ◽

Vol 6 (30) ◽

pp. eaba1685 ◽

Cited By ~ 3

Author(s):

Shiqi Hu ◽

Zhenhua Li ◽

Halle Lutz ◽

Ke Huang ◽

Teng Su ◽

...

Keyword(s):

Hair Follicle ◽

Hair Growth ◽

Dermal Papilla ◽

Hair Follicles ◽

Hair Cycle ◽

Paracrine Signaling ◽

Hair Regrowth ◽

Study Results ◽

Anagen Phase ◽

Hair Follicle Cycle

The progression in the hair follicle cycle from the telogen to the anagen phase is the key to regulating hair regrowth. Dermal papilla (DP) cells support hair growth and regulate the hair cycle. However, they gradually lose key inductive properties upon culture. DP cells can partially restore their capacity to promote hair regrowth after being subjected to spheroid culture. In this study, results revealed that DP spheroids are effective at inducing the progression of the hair follicle cycle from telogen to anagen compared with just DP cell or minoxidil treatment. Because of the importance of paracrine signaling in this process, secretome and exosomes were isolated from DP cell culture, and their therapeutic efficacies were investigated. We demonstrated that miR-218-5p was notably up-regulated in DP spheroid–derived exosomes. Western blot and immunofluorescence imaging were used to demonstrate that DP spheroid–derived exosomes up-regulated β-catenin, promoting the development of hair follicles.

Download Full-text

Impaired Hair Growth and Wound Healing in Mice Lacking Thyroid Hormone Receptors

PLoS ONE ◽

10.1371/journal.pone.0108137 ◽

2014 ◽

Vol 9 (9) ◽

pp. e108137 ◽

Cited By ~ 11

Author(s):

Constanza Contreras-Jurado ◽

Laura García-Serrano ◽

Mónica Martínez-Fernández ◽

Lidia Ruiz-Llorente ◽

Jesus M. Paramio ◽

...

Keyword(s):

Wound Healing ◽

Thyroid Hormone ◽

Hair Growth ◽

Hormone Receptors ◽

Thyroid Hormone Receptors

Download Full-text

Unwanted hair growth induced by topical epidermal growth factor during wound healing: true or myth?

International Wound Journal ◽

10.1111/iwj.12354 ◽

2014 ◽

Vol 13 (5) ◽

pp. 1036-1037 ◽

Cited By ~ 2

Author(s):

Moo Yeol Hyun ◽

Jang Mi Suk ◽

Kwang Ho Yoo ◽

Beom Joon Kim ◽

Myeung Nam Kim ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Hair Growth ◽

Epidermal Growth

Download Full-text

Heme oxygenase-1 in neovascularisation: A diabetic perspective

Thrombosis and Haemostasis ◽

10.1160/th09-12-0825 ◽

2010 ◽

Vol 104 (09) ◽

pp. 424-431 ◽

Cited By ~ 28

Author(s):

Anna Grochot-Przeczek ◽

Jozef Dulak ◽

Alicja Jozkowicz

Keyword(s):

Wound Healing ◽

Heme Oxygenase ◽

Tissue Regeneration ◽

Hair Growth ◽

Tumour Progression ◽

Heme Oxygenase 1 ◽

Blood Vessel Formation ◽

Physiological Processes ◽

Healing Tissue

SummaryNeovascularisation is crucial both for physiological processes, like development, wound healing, tissue regeneration, hair growth or menstrual cycle, and for pathological states, such as tumour progression, retinopathy and psoriasis. Blood vessel formation is orchestrated by numerous pro-angiogenic and anti-angiogenic factors, acting together to keep tight rein on this complicated, desirable but also dangerous process. One of the proteins important for neovascularisation is heme oxygenase-1 (HO-1), an enzyme degrading heme. This review focuses on the role of HO-1 in angiogenesis and vasculogenesis, having a closer look at the significance of this system in diabetes.

Download Full-text