scholarly journals Az évszak és az azonos évszakban történt ismételt mintavételek hatása holstein tenyészbikák ejakulátumának minőségi- és mennyiségi jellemzőire

2002 ◽  
pp. 33-37
Author(s):  
János Tőzsér ◽  
Miklós Mézes ◽  
József Várszegi ◽  
Ferenc Szász
Keyword(s):  
Sperm Quality ◽  
Seasonal Effects ◽  
Seasonal Effect ◽  
Sperm Cells ◽  
Sperm Production ◽  
Special Effect ◽  
Special Effects ◽  
Holstein Friesian ◽  
Multiple Analysis

This study was carried out at the Hungarian National Artificial Insemination Centre in order to evaluate the seasonal effects on the quantitative and qualitative parameters of sperm production using sperm analyser equipment. The volume of ejaculate (VE), percentage of living sperm (PLS), spermatozoa concentration (SC), motility score (MC), percentage of motile spermatozoa (PMS), speed of movement of sperm cells (SMS) and the percentage of sperm cells moving straightforward (PSMS) were collected and observed in three seasons (summer, autumn, winter) in Holstein-Friesian breeding bulls (n = 15). The sperm collection was done for three successive weeks in every season on the same day (summer: n = 41, autumn: n=39, winter: n = 42, altogether: n = 122). The same sperm samples were measured by sperm analyser equipment (HTM version 7.0, Danvers) for PMS, SMS and PSMS. The seasonal and the number of sperm collection effects were confirmed on semen characteristics by multiple analysis of variance (two ways MANOVA, Type III) using the program package of STATISTICA 4.5. The average values of VE were similar in the three seasons (summer: from 4.42 to 6.28 cm3, autumn: from 4.08 to 6.86 cm3, winter: from 5.43 to 5.71 cm3). The average values of the MC were similar in each of the three seasons (summer: from 3.66-4.00 M; autumn: from 3.66-3.77 M; winter: from 3.86-4.07 M). The summary of all effects for season (P<0.001), repeated sperm collections in the same season (P<0.05) and interaction of two traits (P<0.01) were established on the measured characteristics. The special effects (P<0.05) of a given season were calculated, excepting the VE and MC, on all of the measured characteristics. The special effect (P<0.01) of the repeated sperm collections in the same season was verified in only one case (SMS). It is not surprised at the interaction was established on the VE (P<0.001), PMS (P<0.05), and SMS (P<10.0). The results of present study suggest that seasonal effect on sperm quality of breeding bulls cannot be eliminated even at standard feeding and keeping conditions. Considering our results, we can recommend that the average values of three successive weeks in every season be used, to take advantage of seasonal effect on the quantitative and qualitative parameters of sperm production.

scholarly journals Evaluation of Holstein stud bulls by productivity and quality of sperm

2020 ◽  
Vol 23 (11) ◽  
pp. 28-38
Author(s):  
Liudmyla Piddubna ◽  
Daria Zakharchuk ◽  
Ruslan Bratushka
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Individual Characteristics ◽  
Productivity Index ◽  
Strong Positive Correlation ◽  
Sperm Production ◽  
Freezing Resistance ◽  
High Quality ◽  
Important Indicator

The sperm quality of stud bulls is an important indicator on which depends the result of cattle reproduction. The aim of the study was to evaluate the Holstein bulls in terms of productivity and quality of sperm in the conditions of LLC “Ukrainian Genetic Company”. The study was carried out on livestock of 20 stud bulls of the Holstein breed of foreign selection, which were in the same conditions of feeding, keeping and use. The sperm production parameters were determined using an IVOS sperm analyser and evaluated in accordance with respective DSTU standard. Freezing resistance of sperm was estimated as a percentage ratio of sperm doses rejected after cryopreservation to their total amount. The absolute sperm efficiency of stud bulls was evaluated by the method of the experts of the Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science. It was found that that stud bulls of LLC “Ukrainian Genetic Company” are characterized by sufficient fertility. They have produced, on average, 156 ejaculates within a year, which included 106 high-quality ejaculates (67.9%). Characteristics of sperm efficiency of experimental stud bulls are rather variable (Сv from 23.1-79.7%), and vary within the following limits: the number of high-quality ejaculates throughout a year – 32-173 pcs., native sperm obtained – 201-1016 ml, percent of rejected sperm – 3.1-76.1, doses of sperm obtained – 5755-61920 pcs. It was revealed that probable difference in sperm production indicators of sperm providers is associated with their individual characteristics. Volume of ejaculate ranges from 3.77 to 7.30 ml (d=3.57; td=16.6; P<0.001), sperm motility ranges from 7.2 to 8.3 points (d=1.1; td=11.1; P<0.001), sperm concentration in ejaculate ranges from 1.51 to 3.52 bln/ml (d=2.01; td=24; P<0.001), freezing resistance of sperm ranges from 59.6 to 99.6%. Holstein stud bulls of LTD “Ukrainian Genetic Company” have rather high sperm productivity index that ranges from 5.19 to 15.29 bln of motile spermatozoids in ejaculate. A strong positive correlation was found between motility and sperm concentration (r = +0.825; P<0.001), motility and the quantity of sperm doses obtained per year (r = +0.766; P<0.001), concentration and quantity of sperm doses obtained per year (r = +0.595; P<0.001)

Comparison of sperm quality of Belgian Blue and Holstein Friesian bulls

2006 ◽  
Vol 66 (8) ◽  
pp. 1834-1846 ◽  
Author(s):  
G. Hoflack ◽  
G. Opsomer ◽  
A. Van Soom ◽  
D. Maes ◽  
A. de Kruif ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Holstein Friesian

scholarly journals Seasonal effect on semen availability and quality of racing pigeon in Thailand

2021 ◽  
pp. 1459-1464
Author(s):  
Suwarak Wannaratana ◽  
Em-on Olanratmanee ◽  
Kuekaroon Charoenmuang ◽  
Thanvarath Boriharnthanawuth ◽  
Banpatee Tangtrongwanich ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Seasonal Effect ◽  
Seasonal Temperature ◽  
Success Rates ◽  
Average Temperature ◽  
Semen Collection ◽  
Geographical Regions

Background and Aim: Seasonal variations among geographical regions could influence pigeon semen quality differently. This study aimed to determine the seasonal effect on semen availability and quality of racing pigeons in Thailand to understand and improve breeding management in the country. Materials and Methods: Semen was collected from six fertile captive pigeons once a week during summer (March-June), monsoon (July-October), and winter (November-February) during 2019-2020. The success rate of semen collection and semen quality was determined in each season – by which changes in average temperature, humidity, and photoperiod were obtained. Results: Comparable success rates of semen collection were acquired among different seasons, while varied semen qualities were revealed. The percentages of total motility and progressive motility score of sperm were significantly lowest in summer (66.35±3.40 and 3.88±0.15, respectively) compared to monsoon (85.45±2.91 and 4.67±0.10, respectively) and winter (79.29±1.96 and 4.37±0.10, respectively), while its concentration (×109 sperm/mL) and outputs (×106 sperm) were significantly highest in winter (7.62±0.54 and 91.44±10.83, respectively) compared to summer (4.23±0.41 and 48.45±6.35, respectively) and monsoon (3.57±0.30 and 51.45±7.21, respectively). Besides, semen samples collected from birds housing at an average temperature of <29.5°C demonstrated better sperm motility sperm concentration and total sperm counts than those from at a higher temperature. Conclusion: Winter was regarded as the best season contributing the best semen quality, while summer was the worst. Due to the fluctuation of temperature during summer and winter, the seasonal temperature was implied as the major factor contributing to changes in sperm quality of racing pigeons in Thailand.

scholarly journals Differentiation in body and testes weight as well as sperm quality of male roe deer from the Lublin Upland in the reproductive season

2017 ◽  
Vol 73 (7) ◽  
pp. 429-433
Author(s):  
Marian Flis ◽  
Zygmunt Wrona Zbigniew Nozdryn-Płotnicki
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Roe Deer ◽  
Sperm Quality ◽  
Age Groups ◽  
Body Weight Loss ◽  
Sperm Cells ◽  
Hunting Season ◽  
Physiological Processes

The aim of the study was to evaluate the morphology structure of sperm cells and quality of individual male deer obtained in the Lublin Upland during the 2015/2016 hunting season. The comparison included two sepa-rate periods of the hunting season resulting from varying physiological processes related to the estrus period of this species. In the first study with regards to the estrus period, males were characterized by an average of 1.6 grams less testicle weight, while body weight was 1.9 kg higher. Increasing the mass of the testicles in the repro-ductive season proceeded simultaneously with the increase in the share of properly developed sperm cells to the level of 70.6%. During the pre-estrus period, the percentage of spermatozoids with main and secondary defects accounted for almost 60%. Between the compared periods, an approximately 6.8% body weight loss occurred. The highest percentage of properly developed spermatozoids was present in deer aged 4 and 5 years. Regarding the greatest body weight loss in these two age groups, it is indicated that this age is the climax in terms of poten-tial accession to the breeding and effective mating of females and the possibility of fertilization. Analysis of the relationship between body weight and testicle weight showed low and statistically insignificant correlation coef-ficients (rxy=0.331-0.393) between those features.

scholarly journals Testicle size as indicator of fertility in bulls

2014 ◽  
Vol 68 (5-6) ◽  
pp. 311-322
Author(s):  
Igor Prka ◽  
Branislava Djordjevic
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Sperm Production ◽  
Production Potential ◽  
Holstein Friesian ◽  
Selection For ◽  
One Year

Male calves from the high value parents, bull fathers and bull dams, enter the selection for artificial insemination. After laboratory tests, the calves are taken to the center for artificial insemination, and after a stay in quarantine the are moved to a test station. At the age of twelve months they are measured for assessing the value of each calf exterior. One of the measures recorded was the testicle scope. On the basis of testicle size, it is possible to predict sperm production potential. For the determination of testicle size (testicular biometry), tapes or rulers were used. The aim of this work was to investigate the possible effect of testicle size on sperm production in young bulls used for artificial insemination. For that purpose there were used the data on circumference of testicles of one year old bulls just starting production of sperm, and then compared with certain semen quality parameters such as: volume of ejaculate and concentration and percentage of alive and progressively mobile spermatozoa. The investigation included all young bulls that started production in the period from 2010. to 2012., that is 36 bulls of various breeds (Simmental, Holstein Friesian, Montafon). After the testicle scope measuring in these bulls, there were observed the parameters of the sperm quality during the following one year period. The obtained results showed that the increased testicle size was followed by the increased average ejaculate quantity, in other words: 3.7 ml in group of bulls with testicle circumference below 30 cm, to 6.7 ml in bulls whose testicle circumference was over 40 cm. Also, the results showed that there was a correlation between the increased testicle size and the increased spermatozoa concentration. The values grow to testicle scope of 36 cm, and above that they were still high but with some oscillations. When it came to relation between testicle scope and the percentage of alive and progressively mobile spermatozoa, the trend line showed their positive correlation. The percentage of rejected ejaculates varied from 72% in bulls wit testicle scope below 30 cm to 10% in bulls with testicle scope above 35 cm. On the basis of the results obtained in this work, the conclusion is that testicle size is an indicator of bull fertility; that there is a significant correlation between testicle size and ejaculate volume, and that there is a need to explore a genetic link between testicle size and the fertility of their daughters.

Semen Analysis Profile on Varicocele Patients at Dr. Ramelan Navy Hospital Surabaya during Period 2015

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
TIFFANY WONGSODIHARJO
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Secondary Data ◽  
Married Couples ◽  
Sperm Cells ◽  
Descriptive Research ◽  
Normal Limits

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>: Infertility has affected about 10–15 percent married couples. Almost half of infertility cases have been attributed to men with low sperm motility (asthenozoospermia), low sperm count (oligozoospermia) and abnormal sperm morphology (teratozoospermia). Sperm motility is one of the major determinants of male fertility and is required for successful fertilization. Semen analysis is a method to know the quality of semen. Semen analysis consists of macroscopic and microscopic examination of sperm cells, which are concentration, motility and morphology of sperm cells. </p><p><strong>Objective</strong>:<strong> </strong>This study aimed to obtain the semen analysis profile on varicocele patients at DR. Ramelan Navy Hospital Surabaya during period 2015.</p><strong>Method</strong>: This study used descriptive research design and quantitative method, by using the secondary data were collected from medical records during January – December 2015. <strong>Result</strong>: In this study the semen analysis of 36 patients with varicocele, 3 patients (8,33%) with oligozoospermia, 10 patients (27,78%) with asthenozoospermia, 20 patients (55,55%) with oligoasthenozoospermia, 3 patients (8,33%) with extreme oligoasthenozoospermia. <strong>Conclusion</strong>: This study has concluded that all varicocele patients have abnormalities in semen analysis (none of the sperm quality results within normal limits).

Improvement of Sperm Production in Subfertile Boars by Cordyceps militaris Supplement

2007 ◽  
Vol 35 (04) ◽  
pp. 631-641 ◽  
Author(s):  
Wen-Hung Lin ◽  
Ming-Ta Tsai ◽  
Yuh-Shuen Chen ◽  
Rolis Chien-Wei Hou ◽  
Hsiao-Fang Hung ◽  
...  
Keyword(s):  
Direct Evidence ◽  
Sperm Quality ◽  
Cordyceps Militaris ◽  
Sperm Production ◽  
Normal Morphology ◽  
Treatment Groups ◽  
Semen Volume ◽  
Diet Control

Cordyceps species have been traditionally used for the enhancement of sexual function, however, there is few direct evidence to prove this. We investigated the spermatogenic effect of Cordyceps militaris (CM) by supplementation with CM mycelium to subfertile boars. Seventeen Duroc and 12 Landrace boars (29 to 40 months old) were selected to feed with regular diet (control groups, n = 8 and 6, respectively) or diet supplemented with CM mycelium (treatment groups, n = 9 and 6, respectively) for 2 months. Semen was collected once a week. The quality of fertile sperm (normally greater than 62% of motility and 70% of normal morphology) and the quantity (semen volume, and total sperm number) were compared in these boars. The result showed that sperm production was enhanced significantly at the end of first month ( p < 0.05), peaked at the second month ( p < 0.01) of supplementation with CM and was maintained for 2 weeks after stopping the treatment ( p < 0.01). Plasma cordycepin concentration was detected in boars supplemented with CM but not in the controls. More importantly, the percentages of motile sperm cells and sperm morphology were also improved significantly in most of treated boars during the second month of supplementation ( p < 0.01) and 2 weeks after the treatment ( p < 0.05) as compared to their initial values. These results indicate that supplementation with CM mycelium improves sperm quality and quantity in subfertile boars and may partly support the role of Cordyceps in sexual enhancement.

scholarly journals Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

2021 ◽  
Vol 44 (2) ◽  
pp. 138-145
Author(s):  
A. E. Ancuelo ◽  
M. M. Landicho ◽  
G. A. Dichoso ◽  
P. P. Sangel
Keyword(s):  
Superoxide Dismutase ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Cells ◽  
Activity Levels ◽  
Sod Activity ◽  
Cryopreservation Protocol ◽  
Sexually Mature

Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting in decreased sperm quality. ROS's destructive potential is normally counteracted in sperm by their innate antioxidant system consisting of enzymes, which include superoxide dismutase (SOD). This study aimed to assess the quality of semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation of total sperm motility (%) and determination of SOD activity (U/mL). Here, semen samples were collected from 12 sexually mature IP-Khaki drakes, an improved egg-type breed of Philippine mallard duck, and processed using modified reported cryopreservation procedure for ducks. Results showed that post-thawing total sperm motility averages of 12.04±5.61% using 4.5% DMSO and 13.99±5.28% using 7.0% glycerol were comparable. Moreover, similar SOD activity levels of 0.39±0.18 U/mL with 4.5% DMSO and 0.33±0.21 U/mL with 7.0% glycerol in 2.00 x 108 IP-Khaki sperm cells were also observed. The observed very low intracellular SOD activity indicates severe damage to sperm cells due to cryopreservation, which resulted in a comparably low total sperm motility with either of the cryoprotectants. Thus, the cryopreservation protocol used is not the optimum for IP-Khaki semen based on the observed considerable decline in sperm motility and very low SOD activity after cryopreservation.

scholarly journals Antioxidant Effect of Elamipretide on Bull’s Sperm Cells During Freezing/Thawing Process

2020 ◽  
Author(s):  
Alicja Kowalczyk ◽  
Czerniawska Piątkowska Ewa
Keyword(s):  
Sperm Quality ◽  
Protective Role ◽  
Control Group ◽  
Sperm Cells ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Freezing And Thawing ◽  
Thawing Process

Abstract Background: Spermatozoa are subjected to drastic changes in temperature, ice crystal formation, and diverse types of stresses (chemical, physical, osmotic, and oxidative) during the cryopreservation process, which severely compromise sperm quality and fertility. In this study, we aimed to investigate the protective role of Elamipretide in the cryopreservation of bull's sperm.Materials and methods: The study included 36 healthy Simmental bulls with an average age of 2 ± 0.5 years housed individually in pens. Two ejaculates were collected from each bull using an artificial vagina at 7 a.m. Subsequently, the semen was extended with animal protein–free commercial BIOXcell® extender (IMV Technologies, L’aigle, France) to a final concentration of 160x106 spermatozoa/mL, and rated in terms of motile sperm percentage, progressive motility, viability and abnormality of spermatozoa. Semen samples that showed more than 60% motility and 60% viability, were selected for the experiment. The fresh semen was then divided into five equal fractions. The first fraction was left for the control group (without Elamipretide), to the next were added in succession 0.1; 1; 5; and 10 μM of Elamipretide TFA (Trifluoroacetic) (MedChemExpress, USA). After that semen was subjected to freezing and thawing. Next semen was assessed for motility, viability, and antioxidant activity (SOD, CAT, MDA).Results: It has been shown that a concentration of 5 and 10 μM proved to be the most effective in terms of tested parameters of the quality of sperm cells subjected to cryopreservation.Conclusion: In conclusion, addition of the Elamipretide to the cryopreservation extender significantly improved frozen-thawed sperm cells quality and their function. The results of this study indicate that Elamipretide can be used as a cryoprotective agent to protect cells against the devastating effects of oxidative stress and increasing sperm survival after cryopreservation.

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

Sign in / Sign up

Export Citation Format

Share Document