Phosphate solubilization, synthesis of Indol Acetic Acid and effect on biomass soybean inoculated with Pochonia/  Solubilização de Fosfato, síntese de Ácido Indol Acético e efeito na biomassa de soja inoculada com Pochonia

Soil-plant–microbes relations within the plant rhizosphere are the determinants of plant and soil health, which is important for soil ecological environment for plant-microbe interactions. Plant growth-promoting rhizobacteria (PGPR) are considered to encourage plant growth and development directly or indirectly in soil. PGPR can demonstrate a diversity of characteristics responsible .for influencing plant growth and development. During this study, Twenty four different bacterial isolates were isolated, and detailed morphological, biochemical, and physiological characterizations of those isolates were accomplished. This experiment was performed with the 24 bacterial isolates to see their gram stain test, KOH test, catalase activity, cellulose degradation capability, in dole acetic acid (IAA) production, and phosphate solubilization activities, and also tested for growth within the different arsenic and salt stress conditions and 37°C temperature. Results revealed that among the rhizobacterial isolates, fifteen bacterial isolates were negative and nine was positive in gram reaction, while some were showed high IAA production ability, phosphate solubility capability, and cellulose degradation capacity within the culture media. The isolates were isolated from paddy soils and a few were characterized by a yellow color, flat elevation, and gram-positive, while some were characterized because of the yellowish color with round colony shape, raised elevation, gram-negative, and every one the isolates were positive in catalase production capacity and phosphate solubilization activity which is able to increase the available phosphorus within the soil for plants and also produced indole acetic acid that may use as a hormone to be used in growth enhancement of plants. Hence, these isolates need to be tested further for their effect on arsenic dynamics at the plant rhizosphere, selection of suitable plant species for the bacterial association, bacterial effect on arsenic uptake by plants, and potentials for field applications for sustainable agriculture.

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Indol-3-essigsäure induzierte Calciumionen- abgabe bei Maiskoleoptilzylindern / 3-Indol Acetic Acid Induced Efflux of Calciumions by Maize Coleoptile Sections

Zeitschrift für Naturforschung C ◽

10.1515/znc-1973-11-1229 ◽

1973 ◽

Vol 28 (11-12) ◽

pp. 777-778 ◽

Cited By ~ 14

Author(s):

Boris Janistyn

Keyword(s):

Acetic Acid ◽

Maize Coleoptile ◽

Indol Acetic Acid

Abstract 3-Indol acetic acid, calcium, adenosin-3':5'-monophosphat (c-AMP), maize coleoptile sections

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Growth and Aflatoxin Production by Aspergillus parasiticus in a Medium Containing Plant Hormones, Herbicides or Insecticides

Journal of Food Protection ◽

10.4315/0362-028x-50.12.1044 ◽

1987 ◽

Vol 50 (12) ◽

pp. 1044-1047 ◽

Cited By ~ 3

Author(s):

R. S. FARAG ◽

M. A. EL-LEITHY ◽

A. E. BASYONY ◽

Z. Y. DAW

Keyword(s):

Acetic Acid ◽

Gibberellic Acid ◽

Plant Hormones ◽

Aspergillus Parasiticus ◽

Synthetic Medium ◽

Fungal Growth ◽

Aflatoxin Production ◽

Inhibitory Effect ◽

Indol Acetic Acid ◽

Acid Herbicides

The effect of some widely used plant hormones (indol-3-acetic acid and gibberellic acid), herbicides (gramoxone, stomp and treflan) and insecticides (malathion, actellic and guthion) on Aspergillus parasiticus growth and aflatoxin production in a synthetic medium was studied. Addition of indol acetic acid to the medium increased aflatoxin production more than gibberellic acid. Treflan at 5, 10 and 20 ppm levels caused a highly significant stimulatory effect on A. parasiticus growth and aflatoxin production. In contrast, stomp at 10 and 20 ppm produced the reverse effect. Guthion, an insecticide, caused a marked decrease in fungal growth and aflatoxin production. The inhibitory effect of insecticides under study on both fungal growth and aflatoxin production in effectiveness followed the sequence: guthion>actellic>malathion. At the recommended application rate (10 ppm), with the exception of indol acetic acid and treflan, all compounds suppressed mold growth and aflatoxin production.

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Production de composés indoliques rhizogènes par le ver de terre Lumbricus terrestris

Canadian Journal of Zoology ◽

10.1139/z01-132 ◽

2001 ◽

Vol 79 (11) ◽

pp. 1921-1932 ◽

Cited By ~ 3

Author(s):

Abdellatif El Harti ◽

Mohamed Saghi ◽

J -AE Molina ◽

Gérard Teller

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Acetic Acid ◽

Lumbricus Terrestris ◽

Polar Group ◽

Bean Plants ◽

Indol Acetic Acid ◽

Layer Chromatography ◽

Group Of Fractions

In vitro application of total gross extract of earthworms (Lumbricus terrestris) in diverse dilutions stimulates rhizogenesis in young bean plants (Phaseolus vulgaris). The observed effect is similar to that of indol acetic acid, a well-known growth enhancer in plants, used here as a control in various concentrations. Fragmentation of worm extract by column chromatography results in three groups of fractions. Only the polar group of fractions has a significant rhizogenous effect, which is, however, inferior to that observed in the presence of total gross extract of worms or of indol acetic acid. Gross extract analyses using thin layer chromatography, with appropriate chromatography systems and reagents, revealed that indol acetic acid is not present, but is probably replaced by other indol-derived substances that have a neutral to basic chromatographic behaviour. These presumed indol-derived substances are identified as methyl-tryptophane, serotonin, and hydroxy-indol acetic acid. Analyses using mass spectrometry combined with gas chromatography, following fragmentation and purification of the group of rhyzogenous fractions, have revealed the presence of hydroxy-indol carboxylic acid, which seems to take the form of several isomeres.[Journal translation]

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Optimisasi dan pemurnian IAA yang dihasilkan Rhizobium sp. dalam medium serum lateks dengan suplementasi triptofan dari pupuk kandang Optimization and purification of IAA produced by Rhizobium sp. in latex serum media supplemented with tryptophan from chicken manure

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v76i2.83 ◽

2016 ◽

Vol 76 (2) ◽

Author(s):

Irma KRESNAWATY ◽

Syeda ANDANAWARIH ◽

. SUHARYANTO ◽

. TRI-PANJI

Keyword(s):

Acetic Acid ◽

Chicken Manure ◽

Growth Media ◽

High Concentration ◽

Carbon And Nitrogen ◽

Indol Acetic Acid ◽

Layer Chromatography ◽

Hydrolysis Of ◽

Rhizobium Sp ◽

Direct Hydrolysis

Summary Concentrated latex effluent had not been economically utilized, consequently it had become source of environmental pollution and conflicts with surrounding community. Whereas, the concentrated latex effluent could be used as substrate for microbes growth media due to its high concentration of carbon and nitrogen. One of the economical benefits of growing Rhizobium sp. in this waste is the production of indole acetic acid (IAA) that can be used for plant promotion growth. The aims of this research were to get the optimal IAA production of Rhizobium sp. by optimizing its tryptophan supplementation through hydrolysis of chicken manure and to purify IAA produced using chromatographic method. The use of chicken manure directly caused the browning effect, therefore these experiments were carried out the variation of NaOH 2 N hydrolysis treatments to reduce the effect. Direct hydrolysis as the first media was obtained by mixing latex serum and manure, and then this mixture was hydrolyzed. Meanwhile, separated hydrolysis was done by adding water to manure, being hydrolyzed, and divided to become second and third media. The second media was made by mixing manure hydrolysate and latex serum directly, whereas in third media, hydrolisate was added with alum as coagulating agent. Rhizobium sp. was then inoculated to all media and incubated for 24, 48, and 72 hours in 27-30oC. IAA was analyzed by spectrophotometric method with Salkowsky reagent and Thin Layer Chromatography (TLC). IAA was then extracted with ethyl acetate and purified with silica gel column chromatography. The separated hydrolysis without coagulation (second media) produced the highest IAA concentration, that is 14.40 mg/mL, whereas IAA produced by direct hydrolysis (first media) was 14.13 mg/mL and 0.90 mg/mL for third media during 48 hours. The fractionation result for each mediums showed that the highest IAA distribution in first media was the 12th fraction (38.70%), meanwhile in second media was the 15th fraction (50.25%) and in the third media was the 13th fraction (26.16%). Ringkasan Limbah lateks pekat saat ini belum di-manfaatkan secara ekonomis, bahkan menjadi sumber pencemaran lingkungan dan konflik dengan masyarakat sekitarnya. Padahal limbah lateks pekat dapat digunakan sebagai substrat pertumbuhan mikroba karena memiliki kandungan karbon dan nitrogen yang cukup tinggi. Salah satu nilai ekonomis yang dapat diperoleh dengan ditumbuhkannya Rhizobium sp. pada limbah tersebut, yaitu dihasilkannya asam indol asetat (indol acetic acid/IAA) yang dapat digunakan untuk memacu pertumbuhan tanaman. Penelitian ini bertujuan memperoleh produksi IAA optimal yang dihasilkan Rhizobium sp. dengan asupan triptofan dari hidrolisis pupuk kandang dan memurnikan IAA yang dihasilkan tersebut dengan metode kromatografi. Penggunaan pupuk kandang secara langsung menyebabkan efek pen-cokelatan, maka dilakukan variasi perlakuan hidrolisis dengan NaOH 2 N untuk mengurangi efek tersebut. Hidrolisis langsung sebagai medium pertama diperoleh dengan mencampur serum lateks dan pupuk kandang, sedangkan hidrolisis terpisah dilakukan dengan menambah pupuk kandang dengan air, dan dibagi menjadi medium kedua dan ketiga. Medium kedua dibuat dengan cara langsung mencampur hidrolisat dan serum lateks, sedangkan pada medium ketiga, hidrolisat diendapkan dengan alum sebagai bahan pengendap. Kemudian ke dalam masing-masing medium diinokulasi Rhizobium sp. dan diinkubasi selama 24 ,48, dan 72 jam pada suhu 27-30oC. Analisis IAA dilakukan secara spektrofotometri dengan metode Salkowski dan Kromatografi Lapis Tipis (KLT). IAA diekstraksi menggunakan etil asetat dan dimurnikan dengan kromatografi kolom silika gel. Hidrolisis terpisah tanpa pengendapan (medium kedua) menghasilkan IAA tertinggi, yaitu 14,40 mg/mL, sedangkan hidrolis langsung (medium pertama) menghasilkan IAA sebesar 14,13 mg/mL dan medium ketiga sebesar 0,90 mg/mL selama 48 jam. Hasil fraksinasi untuk masing-masing medium menunjukkan sebaran IAA tertinggi pada medium pertama berada pada fraksi ke-12 (38,70%), sedangkan pada medium kedua pada fraksi ke-15 (50,25%), dan pada medium ketiga ialah fraksi ke-13 (26,16%).

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KARAKTERISASI DAN IDENTIFIKASI BAKTERI RIZOSFER TANAMAN SAWIT JAMBI

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v12i2.11723 ◽

2019 ◽

Vol 12 (2) ◽

pp. 198-206

Author(s):

Khairani Khairani ◽

Fitratul Aini ◽

Hesti Riany

Keyword(s):

Acetic Acid ◽

Oil Palm ◽

Pathogenic Bacteria ◽

Biochemical Characterization ◽

Growth Hormones ◽

Rhizosphere Bacteria ◽

Acid Phosphate ◽

Bacterial Genus ◽

Indol Acetic Acid ◽

Conventional Methods

AbstrakBakteri rizosfer adalah bakteri yang terdapat pada daerah perakaran tanaman yang diketahui memiliki keanekaragaman tinggi. Bakteri rizosfer memiliki berbagai peran seperti menyediakan nutrisi bagi tanaman, melindungi tanaman dari infeksi bakteri patogen, menghasilkan hormon pertumbuhan seperti indol acetic acid, pelarut fosfat, pengikat nitrogen, dan lain-lain. Dengan berbagai kemampuan dan peran tersebut, maka perlu eksplorasi bakteri rizosfer tanaman sawit khususnya di daerah perkebunan agar diketahui kelompok bakteri rizosfer, sehingga dapat dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mengetahui kelompok dan keragaman bakteri rizosfer pada tanaman sawit yang berumur 8, 11, dan 14 tahun. Penelitian dilakukan dengan pendekatan konvensional dengan karakterisasi morfologi dan uji biokimia. Dari umur-umur tersebut, didapatkan 18 isolat yang terdiri dari 11 genus, yaitu Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, dan Streptococcus. Bakteri rizosfer tanaman sawit memiliki keragaman genus yang sama pada umur 8, 11, dan 14 tahun. Genus bakteri yang didapatkan dapat dimanfaatkan sebagai PGPR (Plant Promoting Rhizobacteria).Abstract Rhizosphere bacteria are bacteria found in root areas. Rhizosphere bacteria have various roles such as providing nutrients for plants, protecting plants from infection pathogenic bacteria, producing growth hormones such as indol acetic acid, phosphate solvents, nitrogen binders, and others. By having these various abilities and roles, it is necessary to explore the bacteria of oil palm plants, especially in the plantation areas so that the group of rhizosphere bacteria can be identified and then utilized optimally. This study aims to determine the group and diversity of rhizosphere bacteria in oil palm plants that are 8, 11, and 14 years old. The research was conducted conventionally by observing morphological and biochemical characterization. At those ages, 18 isolates were consisting of 11 genera, namely Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, and Streptococcus through conventional methods. Rhizosphere bacteria in oil palm plants have the same genus diversity at the ages of 8, 11, and 14 years. The bacterial genus obtained can be used as PGPR (Plant Promoting Rhizobacteria).

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Insights Into the Molecular Recognition Mechanism of 5-Hydroxy-Indol Acetic Acid by Imprinted Xero-Gel Materials

Science of Advanced Materials ◽

10.1166/sam.2013.1660 ◽

2013 ◽

Vol 5 (12) ◽

pp. 1940-1947 ◽

Cited By ~ 1

Author(s):

Alejandro Simón de Dios ◽

Alfonso Fernández-González ◽

Rosana Badía-Laíño ◽

Marta Elena Díaz-García

Keyword(s):

Acetic Acid ◽

Molecular Recognition ◽

Recognition Mechanism ◽

Indol Acetic Acid

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Effect of Pseudomonas aeruginosa ZGKD2 on the Growth of Amorpha fruticosa L in Coal Gangue

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.807-809.2023 ◽

2013 ◽

Vol 807-809 ◽

pp. 2023-2026

Author(s):

Yu Xiu Zhang ◽

Pei Li Shi ◽

Qian Zhang

Keyword(s):

Pseudomonas Aeruginosa ◽

Acetic Acid ◽

Plant Growth ◽

Root Length ◽

Phosphate Solubilization ◽

Fresh Weight ◽

Amorpha Fruticosa ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Indole 3 Acetic Acid

The cadmium-resistant Pseudomonas aeruginosa strain ZGKD2 was isolated from gangue pile of coal area. Production of siderophores, indole-3-acetic acid (IAA) and the solubilization of phosphate were observed in the strain. Two types of siderophores were identified by UV spectrophotometer. The highest production of IAA and phosphate solubilization were 2.0 ug/mL and 7.2 ug/mL. The root length, plant height and fresh weight of Amorpha fruticosa L in the substrates of Coal gannue and losses were promoted after inoculation with ZGKD2. These data indicated that Pseudomonas aeruginosa strain ZGKD2 was a plant growth-promoting bacterial (PGPB).

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