scholarly journals Antihyperglycaemic and haematological effects of ethanol extract of Salacia lehmbachii leaf in alloxan-induced diabetic Wistar rats

2021 ◽  
Vol 10 (4) ◽  
pp. 394-400
Author(s):  
Godwin Christian Akuodor ◽  
Pius Monday Udia ◽  
Sylvester Chika Ohadoma ◽  
Ifeanyi M. Obi ◽  
Anthony Uchenna Megwas ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
New Drugs ◽  
Haematological Parameters ◽  
Potential Candidate ◽  
Glucose Levels ◽  
Blood Glucose Levels

Introduction: Salacia lehmbachii is used traditionally for the treatment of diabetes mellitus. This study investigated the hypoglycaemic potentials of ethanol leaf extract of S. lehmbachii and its effects on alloxan-induced diabetic rats’ haematological parameters. Methods: A total of 36 male Wistar rats including normal, diabetic untreated, and diabetic treated ones were used in this study. Diabetes was induced by a single intraperitoneal injection of 150 mg/kg of alloxan. When confirmed diabetes, the rats were orally administered 100 mg/kg, 200 mg/kg, and 400 mg/kg daily of the leaf extract for 21 days. The blood glucose levels and haematological parameters were determined in diabetic treated rats compared with the controls. Results: The ethanol leaf extract of S. lehmbachii significantly reduced the blood glucose levels of alloxan-induced diabetic rats when compared with the diabetic control (P < 0.01). The extract also significantly (P < 0.01) improved the haematological parameters of treated rats at three doses employed. Conclusion: It can be concluded that the ethanol leaf extract of S. lehmbachii possesses antihyperglycaemic properties and could be considered a potential candidate for the development of new drugs in the treatment of anaemic conditions.

scholarly journals Antidiabetic and anti-oxidant activities of the methanol leaf extract of Vernonia amygdalina in alloxan-induced diabetes in Wistar rats

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Adegbolagun T. Adeoye ◽  
Ademola A. Oyagbemi ◽  
Aduragbenro D. Adedapo ◽  
Temidayo O. Omobowale ◽  
Abiodun E. Ayodele ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Biochemical Indices

The methanolic leaf extract of Vernonia amygdalina (MLVA) was assessed to evaluate its antidiabetic potential in rats. Diabetes was induced in male Wistar rats by the administration of alloxan monohydrate at 100 mg/kg of body weight. After 48 h, rats with fasting blood glucose levels of 200 mg/dL and above were considered diabetic and used for the study. The experimental animals were grouped into five groups (A–E) of 10 animals each. Group A rats were non-diabetic normal control, Group B consisted of diabetic control rats that received no treatment, groups C, D and E rats were diabetic rats but treated with glibenclamide, 200 and 400 mg/kg doses of MLVA respectively. Blood samples were collected at days 14 and 28 after induction for haematological and serum biochemical indices such as triglycerides, LDL, cholesterols etc. The intestine was collected and intestinal homogenate was prepared for the antioxidant studies. The extract at 200 mg/kg and 400 mg/kg doses significantly (p < 0.05) reduced blood glucose levels in extract-treated diabetic rats and also significantly increased weight gain in these rats. Most haematological parameters in treated rats experienced, while platelets and neutrophils were decreased. Biochemical indices measured were reduced in MLVA-treated groups compared with diabetic control. Treatment with MLVA also produced significant (p < 0.05) decrease in markers of oxidative stress but increased levels of enzymic and non-enzymic antioxidant markers in intestinal homogenates of treated groups compared with diabetic control. This study showed that V. amygdalina has antihyperglycaemic and in vivo antioxidant effects.

scholarly journals Effectiveness of Graptophyllum pictum (L.) Griff leaf extraction on blood glucose level in alloxan-induced Wistar rat

Food Research ◽  
2020 ◽  
Vol 4 (S3) ◽  
pp. 123-126
Author(s):  
A. Leonoreza ◽  
T. Excelinda ◽  
J. Elnitiarta ◽  
H.S. Heri-Nugroho ◽  
M. Hendrianingtyas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Treatment Group ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Normal Group ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease that always increasing in prevalence. Excessive glucose intake and oxidative stress that can lead to damage to the pancreatic cell or insulin resistance, are such factors that influence the occurrence of diabetes mellitus. Graptophyllum pictum (L.) Griff leaf contains active metabolites such as flavonoid which able to decrease free radical levels. This study aimed to prove the effectiveness of Graptophyllum pictum (L.) Griff leaves extract on blood glucose levels in alloxan-induced Wistar rats. In this study, twenty-five male Wistar rats were divided into five groups. On day 0, the negative control and each treatment group injected with 125 mg/kg dose of alloxan and would be checked on 4th day. On the 4th day after the rats had diabetes, normal group (K-1) was given only standard feed, Negative control (K-2) was given only alloxan intraperitoneally on day 0, first treatment group (K-3) was given 50 mg/kg dose of Graptophyllum pictum (L.) Griff, second treatment group (K-4) was given 100 mg/kg dose of the leaf extract, and the third treatment group (K-5) was given the leaf 200 mg/kg dose of the leaf extract. On the 10th day, fasting blood glucose was taken from the rats. Post-test only group design was used to be the experimental design. There were significant differences in the treatment groups (K-3, K-4, and K-5) compared to the negative control group (K-2) after treatment. The mean of blood glucose levels decreased with the increased doses of Graptophyllum pictum (L.) Griff leaf extract treatment. The treatment groups (K-3, K-4, and K-5) were not significant compared to the normal group (K-1).

scholarly journals EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC

2018 ◽  
Vol 11 (8) ◽  
pp. 300
Author(s):  
Kamadyaapa Davie Rexon ◽  
Gondwe Mavuto Masopera ◽  
Shauli Mathulo ◽  
Sewani Rusike Constance ◽  
Nkeh Chungag Benedicta
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Diabetic Rats ◽  
Control Group ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Leaf Extract ◽  
Antioxidant Effects

  Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

Effect of Aloe vera leaf extract on blood glucose levels in alloxan induced diabetic rats

2016 ◽  
Vol 6 (5) ◽  
pp. 471 ◽  
Author(s):  
Manjunath K ◽  
Bhanu G ◽  
Subash KR ◽  
Tadvi NA ◽  
Manikanta M ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER

2020 ◽  
Vol 7 (2) ◽  
pp. 1-5
Author(s):  
Sukmawati Syarif ◽  
Nurnaningsih Nurnaningsih ◽  
Mamat Pratama
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sample Extract ◽  
Male Wistar Rats ◽  
Muntingia Calabura

Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.

Effects of Aqueous Root Extract of Jatropha curcas on Hyperglycaemic and Haematological Indices in Alloxan-induced Diabetic Rats

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
R A Aladodo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Cell ◽  
Jatropha Curcas ◽  
Diabetic Rats ◽  
Haematological Parameters ◽  
Root Extract ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Aqueous Root Extract

Hypoglycaemic potentials of aqueous root extract of Jatropha curcas (250 and 450 mg/kg body weight of rats) was investigated in alloxan-induced diabetic rats. Effect of the extract on some haematological parameters was also investigated. Thirty albino rats (Rattus norvegicus) of mean weight 165.0 ± 2.50 g were randomly selected into normal and diabetic groups, each with six rats. Diabetic treated rats were placed on 14.2 mg/kg body weight glucophage (reference drug), 250 mg/kg and 450 mg/kg body weight of extract. All treatments were administered orally, once daily for fifteen days. The blood glucose levels were monitored and the haematological parameters like haemoglobin (Hgb), red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), percentage lymphocytes (LYM) and neutrophils (NEUT) were also analyzed. A significant (p<0.05) reduction in fasting Blood Glucose Levels (BGL) was observed in alloxan-induced diabetic rats treated with the extract. There was a significant (p<0.05) reduction in Hgb, PCV, RBC and WBC and its differentials of the diabetic untreated rats while administration of glucophage, 250 and 450 mg/kg body weight of the aqueous extract (Jc250 and Jc450) to diabetic rats significantly (p<0.05) increase all these parameters. These results suggested that the aqueous root extract of Jatropha curcas possesses hypoglycaemic activity and also improved the aberrations in the blood parameters of alloxan-induced diabetic rat.

scholarly journals Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats

2021 ◽  
pp. 39-47
Author(s):  
Aligwekwe A. Ugochukwu ◽  
Idaguko C. Anna
Keyword(s):  
Blood Glucose ◽  
Cardiac Glycoside ◽  
Wistar Rats ◽  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Diabetic Control ◽  
Islet Of Langerhans ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Leaf Extract

Aim: The purpose of this study was to evaluate the antidiabetic effect of ethanolic extracts of Petersianthus macrocarpus leaf and its phytochemical analysis using different solvents. Place and Duration of Study: The study was carried out between March and September in 2018 in the Department of Anatomy, Madonna University, Elele. River State, Nigeria. Materials and Methods: Twenty five Wistar rats, weighing between 200-225g were divided into five groups of five rats.  Group A (Control) while Groups B, C, D and E were induced with diabetes using streptozotocin firstly 35mg/kg b.w; and 2 weeks later 25mg/kg b.w.  Group B (Diabetic control), Group C received 0.5mg/kg b.w of Glibenclamide. Groups D and E received daily 50 and 100 mg/kg b.w of ethanolic leaf extract of P. macrocarpus orally for two weeks. The fasting blood glucose levels were determined weekly for two weeks. At the end of the experiment, the animals were sacrificed and the pancreas was removed for histological procedures. Results: The body weights increased significantly (P<0.05) in 100 mg/kg b.w group when compared to the diabetic control rats weight at the end of the experiment. Also there was a significantly decreased (P<0.05) in blood glucose levels in P. macrocarpus (100mg/kg b.w). The histological section of the pancreas of diabetic control showed eosinophilic material in the islet, shrinkage of the islet of Langerhans while the group treated with 100 mg/kg of extract showed granulated and well prominent pancreatic islet of Langerhans. Phytochemical screening showed methanolic extract of P. macrocarpus leaf having alkaloid, saponin, tannin, phenol, flavonoid, cardiac glycoside, steroids, terpenoids, anthocyanin and anthraquinone. However, cardiac glycosides and steroids were absent in ethanolic extract. Cardiac glycoside and terpenoids were also absent in hexane and acetone extract, while phenol, cardiac glycosides, steroids and anthrocyanin were absent in the water extract. Conclusion: Ethanolic leaf extract of P. macrocarpus ameliorate streptozotocin-induced diabetes in Wistar rats.

scholarly journals Antidiabetic, Antihyperlipidemic and Renal Protective Activities of Garcinia indica Linn. Clusiaceae

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Amol Bhalchandra Deore ◽  
Vinayak Dnyandev Sapakal ◽  
Nilofer S. Naikwade
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Plasma Lipid ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Lipid Levels ◽  
Glucose Levels ◽  
Garcinia Indica ◽  
Blood Glucose Levels

To investigate the antidiabetic, antihyperlipidemic and renal protective activities of the aqueous and ethanol extract of Garcinia indica fruit rinds against alloxan induced diabetes in rats. Wistar rats were made diabetic by a single dose of alloxan hydrate [130 mg/kg i.p.]. After the successful induction of experimental diabetes, rats were divided into five groups each comprising a minimum of six rats. The effects of extracts and glibenclamide on fasting blood glucose, plasma lipid levels and renal profile were examined for 21 days. Blood glucose levels and biochemical parameters such as serum cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, urea and creatinine levels of rats were measured using on weekly intervals i.e day 0, 7, 14 and 21 after daily administration of all extracts at dose of 500 mg/kg. Statistical analysis was performed using Dunnett’s test. p less than 0.01 was taken as the criterion of significance. Oral administration of both aqueous and ethanol extract for 21 days caused a significant [p less than 0.01] reduction in blood glucose levels, lipid profile except HDL; urea and creatinine in diabetic rats. Garcinia indica fruit rind possesses antihyperglycemic activity as well improves total lipid levels and renal profile. It can justify folklore uses of the plant in diabetes.

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