Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

The methanolic leaf extract of Vernonia amygdalina (MLVA) was assessed to evaluate its antidiabetic potential in rats. Diabetes was induced in male Wistar rats by the administration of alloxan monohydrate at 100 mg/kg of body weight. After 48 h, rats with fasting blood glucose levels of 200 mg/dL and above were considered diabetic and used for the study. The experimental animals were grouped into five groups (A–E) of 10 animals each. Group A rats were non-diabetic normal control, Group B consisted of diabetic control rats that received no treatment, groups C, D and E rats were diabetic rats but treated with glibenclamide, 200 and 400 mg/kg doses of MLVA respectively. Blood samples were collected at days 14 and 28 after induction for haematological and serum biochemical indices such as triglycerides, LDL, cholesterols etc. The intestine was collected and intestinal homogenate was prepared for the antioxidant studies. The extract at 200 mg/kg and 400 mg/kg doses significantly (p < 0.05) reduced blood glucose levels in extract-treated diabetic rats and also significantly increased weight gain in these rats. Most haematological parameters in treated rats experienced, while platelets and neutrophils were decreased. Biochemical indices measured were reduced in MLVA-treated groups compared with diabetic control. Treatment with MLVA also produced significant (p < 0.05) decrease in markers of oxidative stress but increased levels of enzymic and non-enzymic antioxidant markers in intestinal homogenates of treated groups compared with diabetic control. This study showed that V. amygdalina has antihyperglycaemic and in vivo antioxidant effects.

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EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.24207 ◽

2018 ◽

Vol 11 (8) ◽

pp. 300

Author(s):

Kamadyaapa Davie Rexon ◽

Gondwe Mavuto Masopera ◽

Shauli Mathulo ◽

Sewani Rusike Constance ◽

Nkeh Chungag Benedicta

Keyword(s):

Blood Glucose ◽

Leaf Extract ◽

Antioxidant Properties ◽

Diabetic Rats ◽

Control Group ◽

Oral Glucose ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Ethanolic Leaf Extract ◽

Antioxidant Effects

Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

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The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.12076 ◽

2019 ◽

Vol 5 (2) ◽

pp. 57

Author(s):

Yesi Warisman ◽

Arinda Lironika Suryana ◽

Zora Olivia

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Control Group ◽

Standard Diet ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel. Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

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Phytochemical Evaluation and Anti-Diabetic Effects of Ethanolic Leaf Extract of Petersianthus macrocarpus on Streptozotocin-Induced Diabetic Rats

Journal of Advances in Medicine and Medical Research ◽

10.9734/jammr/2021/v33i330819 ◽

2021 ◽

pp. 39-47

Author(s):

Aligwekwe A. Ugochukwu ◽

Idaguko C. Anna

Keyword(s):

Blood Glucose ◽

Cardiac Glycoside ◽

Wistar Rats ◽

Cardiac Glycosides ◽

Leaf Extract ◽

Diabetic Control ◽

Islet Of Langerhans ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Ethanolic Leaf Extract

Aim: The purpose of this study was to evaluate the antidiabetic effect of ethanolic extracts of Petersianthus macrocarpus leaf and its phytochemical analysis using different solvents. Place and Duration of Study: The study was carried out between March and September in 2018 in the Department of Anatomy, Madonna University, Elele. River State, Nigeria. Materials and Methods: Twenty five Wistar rats, weighing between 200-225g were divided into five groups of five rats. Group A (Control) while Groups B, C, D and E were induced with diabetes using streptozotocin firstly 35mg/kg b.w; and 2 weeks later 25mg/kg b.w. Group B (Diabetic control), Group C received 0.5mg/kg b.w of Glibenclamide. Groups D and E received daily 50 and 100 mg/kg b.w of ethanolic leaf extract of P. macrocarpus orally for two weeks. The fasting blood glucose levels were determined weekly for two weeks. At the end of the experiment, the animals were sacrificed and the pancreas was removed for histological procedures. Results: The body weights increased significantly (P<0.05) in 100 mg/kg b.w group when compared to the diabetic control rats weight at the end of the experiment. Also there was a significantly decreased (P<0.05) in blood glucose levels in P. macrocarpus (100mg/kg b.w). The histological section of the pancreas of diabetic control showed eosinophilic material in the islet, shrinkage of the islet of Langerhans while the group treated with 100 mg/kg of extract showed granulated and well prominent pancreatic islet of Langerhans. Phytochemical screening showed methanolic extract of P. macrocarpus leaf having alkaloid, saponin, tannin, phenol, flavonoid, cardiac glycoside, steroids, terpenoids, anthocyanin and anthraquinone. However, cardiac glycosides and steroids were absent in ethanolic extract. Cardiac glycoside and terpenoids were also absent in hexane and acetone extract, while phenol, cardiac glycosides, steroids and anthrocyanin were absent in the water extract. Conclusion: Ethanolic leaf extract of P. macrocarpus ameliorate streptozotocin-induced diabetes in Wistar rats.

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Phytochemical Composition and Effect of Nauclea Latifolia Aqueous Extracts on Blood Glucose Levels of Streptozotocin-Induced Diabetic Wistar Albino Rats

Journal of BioMedical Research and Clinical Practice ◽

10.46912/jbrcp.56 ◽

2018 ◽

Vol 1 (2) ◽

pp. 157-163

Author(s):

D O Ochalefu ◽

G I Adoga ◽

C D Luka ◽

H A Abu ◽

E O O Amali ◽

...

Keyword(s):

Blood Glucose ◽

Wistar Rats ◽

Stem Bark ◽

Diabetic Control ◽

Diabetic Rats ◽

Root Bark ◽

Glucose Lowering ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Nauclea Latifolia

Researches targeted at understanding and controlling abnormally high level of blood glucose in Diabetes mellitus are ongoing. In spite of many available anti-diabetic drugs in the market, safer and cheaper remedies from plant material are being sought for due to the unwanted side effects of these drugs. In this research work, we looked at the beneficial blood glucose lowering effect of the plant Nauclea latifolia in streptozotocin-induced diabetic albino Wistar rats. The extracts were first screened for the presence of phytochemicals using standard methods and the result showed the presence of saponins, tannins, reducing sugar, phlobatannins, anthraquinone, flavonoids, steroids and alkaloids. The study design involved 30 male albino Wistar rats which were divided into 6 groups. Groups 1 and 2 represent the Normal Control and Diabetic Control respectively. Groups 3, 4 and 5 were Diabetic rats treated orally with 500mg/kg body weight of aqueous extracts of Stem-bark, Leaves and Root-bark respectively while Group 6 were Diabetic rats treated with 5mg/kg body weight of Glibenclamide. The extracts and Glibenclamide were administered for 28 days. Blood glucose levels were determined on days 0, 7, 14, 21 and 28 by tail tipping method using Glucometer (Accu-Chek, Manheim, Germany). The extracts and Glibenclamide reduced significantly (p<0.05) the Fasting Blood Glucose levels in the diabetic treated rats compared with the Diabetic Control. Amongst the extracts the root-bark is more efficacious, decreasing the glucose level by 49.80% while the stem-bark and leaves brought about reduction by 28.76% and 12.15% respectively. The findings imply that the extracts of this plant have blood glucose lowering ability and should be further evaluated for its beneficial effect in Diabetes mellitus management.

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Antihyperglycaemic and haematological effects of ethanol extract of Salacia lehmbachii leaf in alloxan-induced diabetic Wistar rats

Journal of Herbmed Pharmacology ◽

10.34172/jhp.2021.46 ◽

2021 ◽

Vol 10 (4) ◽

pp. 394-400

Author(s):

Godwin Christian Akuodor ◽

Pius Monday Udia ◽

Sylvester Chika Ohadoma ◽

Ifeanyi M. Obi ◽

Anthony Uchenna Megwas ◽

...

Keyword(s):

Blood Glucose ◽

Wistar Rats ◽

Leaf Extract ◽

Ethanol Extract ◽

Diabetic Rats ◽

New Drugs ◽

Haematological Parameters ◽

Potential Candidate ◽

Glucose Levels ◽

Blood Glucose Levels

Introduction: Salacia lehmbachii is used traditionally for the treatment of diabetes mellitus. This study investigated the hypoglycaemic potentials of ethanol leaf extract of S. lehmbachii and its effects on alloxan-induced diabetic rats’ haematological parameters. Methods: A total of 36 male Wistar rats including normal, diabetic untreated, and diabetic treated ones were used in this study. Diabetes was induced by a single intraperitoneal injection of 150 mg/kg of alloxan. When confirmed diabetes, the rats were orally administered 100 mg/kg, 200 mg/kg, and 400 mg/kg daily of the leaf extract for 21 days. The blood glucose levels and haematological parameters were determined in diabetic treated rats compared with the controls. Results: The ethanol leaf extract of S. lehmbachii significantly reduced the blood glucose levels of alloxan-induced diabetic rats when compared with the diabetic control (P < 0.01). The extract also significantly (P < 0.01) improved the haematological parameters of treated rats at three doses employed. Conclusion: It can be concluded that the ethanol leaf extract of S. lehmbachii possesses antihyperglycaemic properties and could be considered a potential candidate for the development of new drugs in the treatment of anaemic conditions.

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Uji efek ekstrak daun papaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.12291 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Cynthia C.C. Senduk ◽

Henoch Awaloei ◽

Edward Nangoy

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Carica Papaya ◽

Control Group ◽

Elevated Blood ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Sugar Levels

Abstract: Indonesia is a tropical country with more than 9,600 kinds of medicinal plants; one of them is papaya. Extract of papaya leaf (Carica papaya) is presumed to have hypoglycemia effect because it contains flavonoid, alkaloid, saponin, and tannin. This study aimed to evaluate the effect of papaya leaf extract on elevated blood glucose levels on Wistar rats induced with alloxan. This was an experimental study with 18 male Wistar rats as subjects, divided into 6 groups (3 rats in each group). Group 1, the negative control group, was given aquadest only. Group 2, the positive control group, was treated with alloxan 120 mg/kg body weight (BW) followed by novomix 0.2 iu/200 g BW. Group 3 and 4 were treated with alloxan 120 mg/kg BW followed by papaya leaf extract dosing 250 mg and 500 mg/kg BW respectively. Group 5 and 6 were treated with papaya leaf extract dosing 250 mg and 500 mg/kg BW without alloxan induction. Blood glucose levels were measured on day 1, day 2, and day 3 every six hours at 0, 6, 12, 18, and 24 hours. The results showed that 250 mg/kg BW and 500 mg/kg BW of papaya leaf extract could reduce the elevated blood glucose on Wistar rats for 12 hours after treatment. Conclusion: The extract of papaya leaves could reduce blood sugar levels in hyperglicemic Wistar rats induced by alloxan. Keywords: papaya leaves (carica papaya L.), blood sugar levels, alloxan. Abstrak: Indonesia merupakan salah satu negara beriklim tropis yang memiliki banyak jenis tumbuhan. Sekitar 9.600 spesies tumbuhan merupakan tumbuhan yang berkhasiat obat, salah satunya ialah pepaya. Ekstrak daun pepaya (Carica papaya L.) diduga mempunyai efek hipoglikemia karena mengandung flavonoid, alkaloid, saponin, dan tannin. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun pepaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan. Jenis penelitian ini eksperimental. Subyek penelitian yang digunakan ialah 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok (setiap kelompok terdiri dari 3 tikus). Kelompok 1 (K1) merupakan kelompok kontrol negatif hanya diberikan aquades; kelompok 2 (K2) merupakan kelompok kontrol positif diberikan aloksan dan novomix 0,2 iu/200 g BB; kelompok 3 (K3) dan kelompok 4 (K4) merupakan kelompok perlakuan diberikan aloksan kemudian ekstrak daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB; kelompok 5 (K5) dan kelompok 6 (K6) merupakan kelompok perlakuan diberikan daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB tanpa induksi aloksan. Data diperoleh dari pemeriksaan kadar gula darah dari semua kelompok tikus Wistar pada hari ke-1, ke-2, dan ke-3 pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pepaya dengan dosis 250 mg dan 500 mg/kg BB tikus berefek menurunkan kadar gula darah tikus wistar selama 12 jam pasca pemberian ekstrak daun pepaya. Simpulan: Ekstrak daun pepaya berpotensi memiliki efek dalam menurunkan kadar gula darah pada tikus Wistar.Kata kunci: daun pepaya (carica papaya L.), kadar gula darah, aloksan

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Uji efek ekstrak daun sirih hutan (Piper aduncum L.) terhadap kadar gula darah pada tikus wistar (Rattus novergicus) yang diinduksi aloksan

Jurnal e-Biomedik ◽

10.35790/ebm.4.2.2016.14659 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Silvia R.H. Sitinjak ◽

Jane Wuisan ◽

Christi Mambo

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Control Group ◽

Betel Leaf ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Decrease Blood Glucose ◽

Piper Aduncum

Abstract: Forest betel leaf (Piper aduncum L.) has been known bypublicto have efficacy in wound healing, stop vomiting, reducing nausea, aiding digestion, as an antiseptic, as well as killing bacteria, fungi and viruses. Dewi et al. found that 50 mg/kgBW and 100 mg/kgBW red betel leaf extract caused a decrease blood glucose levels in Wistar rats (Rattus norvegicus) induced by alloxan. This study was aimed to identify the effect of forest betel leaf extract on blood glucose levels on Wistar rats induced by alloxan. Subjects were Wistar rats with total of 15 samples divided into five groups: a negative control group and four hyperglycemic groups induced by 130 mg/kgBW of alloxan. Forest betel leaf extract was administered with dose of 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW on each groups of hyperglycemic rats, and group0.4 IU/100grBW of novomix injection was administered on the positive control group. Blood glucose levels were measured in the 0, 30, 60, 90, and 120 minutes on day one, two, and three. The results showed that 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW of forest betel leaf extract had a tendency to decrease blood glucose levels of Wistar rats induced by alloxan.Keywords: piper aduncum L., forest betel leaf, blood glucose levels, alloxan Abstrak: Daun sirih hutan (Piper aduncum L.) telah dikenal oleh masyarakat dan mempunyai khasiat dalam penyembuhan luka, menghentikan muntah, mengurangi mual, melancarkan pencernaan, sebagai antiseptik, membunuh bakteri dan jamur serta virus. Menurut penelitian Dewi dkk, pemberian ekstrak daun sirih merah dengan dosis 50 mg/kgBB dan 100 mg/kgBB menyebabkan penurunan kadar gula darah pada tikus Wistar (Rattus norvegicus) yang diinduksi dengan aloksan. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun sirih hutan terhadap kadar gula darah pada tikus Wistar yang diinduksi dengan aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok terdiri atas kelompok kontrol negatif dan 4 kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB tikus yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB tikus, dan 100 mg/kgBB tikus, dan yang terakhir kelompok kontrol positif diberi suntikan novomix 0,4 IU/100grBB tikus. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok pada hari pertama, kedua, dan ketiga pada menit ke-0, 30, 60, 90, dan 120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB dan 100 mg/kgBB tikus memiliki kecenderungan untuk menurunkan kadar gula darah tikus Wistar yang diinduksi oleh aloksan. Kata kunci: piper aduncumL., daun sirih hutan, kadar gula darah, aloksan

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Effectiveness of Graptophyllum pictum (L.) Griff leaf extraction on blood glucose level in alloxan-induced Wistar rat

Food Research ◽

10.26656/fr.2017.4(s3).s12 ◽

2020 ◽

Vol 4 (S3) ◽

pp. 123-126

Author(s):

A. Leonoreza ◽

T. Excelinda ◽

J. Elnitiarta ◽

H.S. Heri-Nugroho ◽

M. Hendrianingtyas ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Treatment Group ◽

Wistar Rats ◽

Leaf Extract ◽

Negative Control ◽

Normal Group ◽

Treatment Groups ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus is a metabolic disease that always increasing in prevalence. Excessive glucose intake and oxidative stress that can lead to damage to the pancreatic cell or insulin resistance, are such factors that influence the occurrence of diabetes mellitus. Graptophyllum pictum (L.) Griff leaf contains active metabolites such as flavonoid which able to decrease free radical levels. This study aimed to prove the effectiveness of Graptophyllum pictum (L.) Griff leaves extract on blood glucose levels in alloxan-induced Wistar rats. In this study, twenty-five male Wistar rats were divided into five groups. On day 0, the negative control and each treatment group injected with 125 mg/kg dose of alloxan and would be checked on 4th day. On the 4th day after the rats had diabetes, normal group (K-1) was given only standard feed, Negative control (K-2) was given only alloxan intraperitoneally on day 0, first treatment group (K-3) was given 50 mg/kg dose of Graptophyllum pictum (L.) Griff, second treatment group (K-4) was given 100 mg/kg dose of the leaf extract, and the third treatment group (K-5) was given the leaf 200 mg/kg dose of the leaf extract. On the 10th day, fasting blood glucose was taken from the rats. Post-test only group design was used to be the experimental design. There were significant differences in the treatment groups (K-3, K-4, and K-5) compared to the negative control group (K-2) after treatment. The mean of blood glucose levels decreased with the increased doses of Graptophyllum pictum (L.) Griff leaf extract treatment. The treatment groups (K-3, K-4, and K-5) were not significant compared to the normal group (K-1).

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BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i4.23502 ◽

2018 ◽

Vol 11 (4) ◽

pp. 257

Author(s):

Mutiara Indah Sari ◽

Maya Anjelir Antika ◽

Dwi Rita Anggraini

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Ethanolic Extract ◽

Wistar Rat ◽

Diabetic Control ◽

Diabetic Rats ◽

Control Group ◽

Lawsonia Inermis ◽

Control Group Design ◽

Glucose Levels

Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

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