scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER

2020 ◽  
Vol 7 (2) ◽  
pp. 1-5
Author(s):  
Sukmawati Syarif ◽  
Nurnaningsih Nurnaningsih ◽  
Mamat Pratama
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sample Extract ◽  
Male Wistar Rats ◽  
Muntingia Calabura

Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

Antidiabetic Activity of Peperomia pellucida In Streptozotocin-Induced Diabetic Mice

2021 ◽  
Vol 7 (2) ◽  
pp. 120-129
Author(s):  
Sholihatil Hidayati
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Potential Development ◽  
Lower Blood ◽  
Development Objectives

Background: Diabetes mellitus is a heterogeneous group of diseases in the form of disorders in the body's metabolism clinically. Peperomia pellucida herbs have phytochemical containing which is antidiabetic potential development. Objectives: This study was conducted to compare the antidiabetic activity of ethanol extract and n-hexane fraction of Peperomia pellucida. Material and Methods: This research was conducted by make diabetic mice with 50 mg/kg.bw of streptozotocin induction, which was then treated with ethanol extract and n-hexane fraction of Peperomia pellucida with doses 250 mg/kgbw for 7 days.  Results: The results showed that the ethanol extract and n-hexane fraction of Peperomia pellucida reduced blood glucose levels in diabetic mice due to streptozotocin induction. The n-hexane fraction of Peperomia pellucida can lower blood glucose levels as much 244.00 ± 18.99 mg/dL better than the ethanol extract, which is 99.50 ± 28.17 mg/dL. Conclusions: Peperomia pellucida herb has the potential to be developed as an antidiabetic agent.

scholarly journals Phytochemical Screening and Antidiabetic Activity Test of Extracts and Fractions of Lactuca Indica (L.) In Streptozotocin-Induced Diabetic Mice

1970 ◽  
Vol 8 (3) ◽  
pp. 62-65
Author(s):  
Tamba Rosanto ◽  
Nainggolan Marline ◽  
Rosidah Noersal
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Reduce Blood Glucose ◽  
Lactuca Indica

Objectives: The purpose of this study was to determine the antidiabetic effect of ethanol extract and Lactuca indica leaf fraction to determine the effective dose of mice induced with streptozotocin compared with metformin. Design: The design of this study was experimental in which the extraction and fraction of Lactuca indica leaves were tested for the value of decreasing blood glucose levels of mice after induction of streptozotocin. Antidiabetic activity tests were divided into 12 groups. Group I (baseline) group II (negative control) were given CMC 0.5%, Group III (positive control) were given metformin 65 mg/kg BW, while Groups IV to XII were given Lactuca indica leaf extract and fractions at their respective doses -each 100, 150 and 200 mg/kg BW Interventions: The variable that was intervened in this study was the concentration of extract used Main outcome measure: The main measurement results in this study were to determine extracts and fractions that we're able to reduce blood glucose levels in mice. Results: The antidiabetic effect of Lactuca indica leaves shows that EELL, EAFLL and NHFLL have antidiabetic effects, this is supported by the chemical content contained in the extracts, namely flavonoids, tannins, saponins, glycosides and triterpenoids/steroids. The most effective activity to reduce blood glucose levels in streptozotocin-induced mice is EAFLL 100 mg/kg BW Conclusion: ethyl acetate fraction of Lactuca indica leaves has an effective antidiabetic activity at a dose of 200 mg/kg BW has given antidiabetic activity in mice induced by STZ, on the 9th day BGL mice have dropped below 120 mg/dl.  

scholarly journals NANOEMULSION OF OKRA FRUIT EXTRACT AS ANTIDIABETIC TREATMENT

2020 ◽  
pp. 138-142
Author(s):  
RATNA DJAMIL ◽  
SARAH ZAIDAN ◽  
DENI RAHMAT ◽  
DIAH KARTIKA PRATAMI ◽  
FELIX HAKIM
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Glucose Levels ◽  
Antidiabetic Treatment ◽  
Blood Glucose Levels ◽  
Average Blood Glucose

Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice. Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated in a solution of glyceryl caprylate, propylene glycol, and glycerine to form a nanoemulsion. To determine the antihyperglycaemic effect of okra extract, 35 male mice (Mus musculus L.) were divided into seven groups: a non-diabetic normal control group and six diabetic mice groups (untreated negative control, glibenclamide-treated positive control, and four treatments with okra ethanol extract (OEE) at 200 and 400 mg/kg BW and NOE at 200 and 400 mg/kg BW). Results: The group treated with NOE at 400 mg/kg BW (NOE400) had the lowest average blood glucose level of 93.4 mg/dL among hyperglycaemic mice. The decrease in blood glucose levels in NOE400 (52.05%) was significantly different from those in the positive control (42.63%) and OEE treatments (39.32%). The nanoemulsion used in this study fulfills quality requirements, with a mean particle size of 134.7 nm, a polydispersity index of 0.512, and a zeta value of −26.72 mV. Conclusion: NOE reduced blood glucose levels in alloxan-induced hyperglycaemic mice better than OEE. Nanoemulsion can improve the antidiabetic activity of okra extract by increasing penetration of active compounds into interstitial space so that their delivery and bioavailability are higher.

scholarly journals RESPON AKTIVITAS TIKUS WISTAR JANTAN AKIBAT KONDISI DIET TINGGI SUKROSA DIUKUR MENGGUNAKAN PEREKAM AKTIVITAS

Jurnal BIOMA ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 104
Author(s):  
Yanuar Restu Wijaya ◽  
Koekoeh Santoso ◽  
Isdoni Isdoni ◽  
Atin Supiyani
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Feeding Activity ◽  
Control Group ◽  
High Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Track System ◽  
Male Wistar Rats ◽  
Auto Track

Excessive energi consumption play a role in increasing blood glucose levels (hyperglycemia), due to the inability of the hormone insulin to compensate the high blood glucose levels. Blood glucose receptor found in the hypothalamus of VMH (Ventro Medial Hypothalamus) and LH (the Lateral Hypothalamus) is able to detect and change the settings for the feeding activity. The aim of this study was to obtain the relationship between high sucrose feeding on male Wistar rats of behavior and activity. This method uses Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% sucrose, the second group was given additional feed 40% sucrose, a third group was given additional feed 60% sucrose, and the last as a control group. This feed given continuously for 70 days.Observation of activities conducted using Opto-varimex® auto-track system ver.4.31. The results showed an corellation between activity based doses of sucrose.

scholarly journals Antidiabetic activities of 96% ethanol extract of Abelmoschus esculentus on Balb-C mice strain

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Agnis Pondineka Ria Aditama
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Abelmoschus Esculentus ◽  
Antidiabetic Activity ◽  
Male Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

Diabetes mellitus (DM) is a progressive metabolic disease that continues to increase every year. Treatment therapy using oral hypoglycemic drugs to treat DM has side effects, and alternative approaches must be taken. Abelmoschus esculentus has been known to be used as an alternative to DM because it has antioxidant activity due to its high flavonoid content. The purpose of this study was to determine the antidiabetic activity of 96% ethanol extract of Okra leaves against male mice of balb-c strain. Male mice were acclimatized for seven days and divided into five test groups and then fasted for 18 hours. Then the mice were induced with 150 mg / 20 g BW alloxan and measured on the 3rd day. Ethanol extract 96% okra leaves were then given in a dose of 5.6 mg / 20g BW; 11.2 mg / 20g BW; 22.4 mg / 20g BW, CMC Na 0.5% as negative control and glibenclamide 0.026 mg / 20g BBs as positive control for 14 days. The results of therapy with 96% ethanol extract of okra leaves can reduce blood glucose levels in mice so that the okra leaves contain flavonoid compounds that have antioxidant activity and can reduce blood glucose levels.

scholarly journals Antidiabetic Activity of Sungkai (Peronema canescens Jack) Leaves Ethanol Extract on the Male Mice Induced Alloxan Monohydrate

2021 ◽  
Vol 6 (2) ◽  
pp. 64
Author(s):  
Madyawati Latief ◽  
Putri M. Sari ◽  
Liddini T. Fatwa ◽  
Indra L. Tarigan ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Blood Glucose Measurement ◽  
Antidiabetic Activity ◽  
Glucose Measurement ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

  Sungkai (Peronema canescens Jack) is an alternative natural ingredient that can be used as an antidiabetic containing several secondary metabolites which are thought to reduce blood glucose levels by inhibiting the action of the α-glucokinase enzyme and increasing the activity of antioxidant enzymes and being able to regenerate β-cells, damaged pancreas so that insulin deficiency can be overcome. The aim of this study was to determine the effect of P. canescens leaves ethanol extract on antidiabetic activity and to determine the effect of various doses of sungkai leaf ethanol extract as antidiabetic. The scientific research method used in this study was a completely randomized design (CRD) and data analysis used in this study is the one way ANOVA, following by the Duncan test. Blood glucose measurement in treated mice was carried out 3 times. Mice were devided into five groups, three mice in each group, and each group was administered different dosages of sungkai extract (T1 = extract 175 mg/kg body weight; T2 = extract 350 mg/kg body weight; T3 = extract 700 mg/ kg body weight). First, the mice were acclimatized for six days and the injection of treatment was started at day 7. Blood glucose levels, urine volume, body weight and daily drinking of mice was carried out before induction (day 0), after induction (day 8) and after the end of treatment for 10 days (day 18), was carried out using the Point of Care Test (POCT) method using the Easy Touch GCU. The results showed that the ethanol extract of sungkai leaves had the potential to reduce blood glucose levels in mice. The ethanol extract of sungkai leaves at a dose of 350 mg/kg body weight has the most optimal potential to reduce blood glucose levels, urine volume, daily drinking, and body weight. 

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

scholarly journals UJI AKTIVITAS ANTIHIPERGLIKEMIA EKSTRAK ETANOL 70% Echinometra mathaei PADA TIKUS PUTIH JANTAN GALUR WISTAR YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 2 (1) ◽  
pp. 29
Author(s):  
Angelica Kresnamurti ◽  
Nana Angelica ◽  
Farizah Izazi
Keyword(s):  
Blood Glucose ◽  
Sea Urchin ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Standard Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Echinometra Mathaei

Abstract : Sea urchin (Echinometra mathaei) contains a compound of Echinochrome A, which is a polyhydroxy naphthoquinone that is known to be potential antioxidant. The antioxidant effect correlated with the antidiabetic activity that associated with oxidative stress conditions. The aim of this study was to determine the effect of 70% Ethanol Extract of sea urchin (Echinometra mathaei) on blood glucose levels in alloxan induced rats. This research was an experimental study using 30 male white rats, divided into 6 groups, i.e the negative control group (normal rat were only given CMC Na 0,5%), Positive control group (hyperglycemic rats were only treated with CMC Na 0,5%), metformin group as a refference standard group (hyperglycemic rats that treated with metformin) and the treatment group (hyperglycemic rats were treated with ethanol extract 70% of Echinometra mathaei dose of 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Alloxan monohidrat 120 mg/KgBW was used as diabetogen agent. The parameters examined in this study were blood glucose levels of rats after 7 days of treatment. The observational data were analyzed using the One Way ANOVA (P < 0.05) and continued with LSD. The results of this study showed that all doses of ethanol extract significantly reduced blood glucose levels in rats compared to the control group. The most effective dose in reducing blood glucose levels and compared to metformin was 400 mg / kgBW. Key word: alloxan induced, Echinometra mathaei, ethanol 70%, hiperglycemia, marine product.

scholarly journals Antihyperglycaemic and haematological effects of ethanol extract of Salacia lehmbachii leaf in alloxan-induced diabetic Wistar rats

2021 ◽  
Vol 10 (4) ◽  
pp. 394-400
Author(s):  
Godwin Christian Akuodor ◽  
Pius Monday Udia ◽  
Sylvester Chika Ohadoma ◽  
Ifeanyi M. Obi ◽  
Anthony Uchenna Megwas ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
New Drugs ◽  
Haematological Parameters ◽  
Potential Candidate ◽  
Glucose Levels ◽  
Blood Glucose Levels

Introduction: Salacia lehmbachii is used traditionally for the treatment of diabetes mellitus. This study investigated the hypoglycaemic potentials of ethanol leaf extract of S. lehmbachii and its effects on alloxan-induced diabetic rats’ haematological parameters. Methods: A total of 36 male Wistar rats including normal, diabetic untreated, and diabetic treated ones were used in this study. Diabetes was induced by a single intraperitoneal injection of 150 mg/kg of alloxan. When confirmed diabetes, the rats were orally administered 100 mg/kg, 200 mg/kg, and 400 mg/kg daily of the leaf extract for 21 days. The blood glucose levels and haematological parameters were determined in diabetic treated rats compared with the controls. Results: The ethanol leaf extract of S. lehmbachii significantly reduced the blood glucose levels of alloxan-induced diabetic rats when compared with the diabetic control (P < 0.01). The extract also significantly (P < 0.01) improved the haematological parameters of treated rats at three doses employed. Conclusion: It can be concluded that the ethanol leaf extract of S. lehmbachii possesses antihyperglycaemic properties and could be considered a potential candidate for the development of new drugs in the treatment of anaemic conditions.

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