scholarly journals In vitro comparison of two photostimulable phosphor plate systems for early detection of occlusal dentin caries with and without a sharpening filter

2020 ◽  
Vol 14 (4) ◽  
pp. 223-227
Author(s):  
Roghieh Bardal ◽  
Mahshid Mobini ◽  
Matin Mirzaee
Keyword(s):  
Statistical Significance ◽  
Occlusal Caries ◽  
Caries Diagnosis ◽  
Dentin Caries ◽  
Significant Difference ◽  
Photostimulable Phosphor ◽  
Phosphor Plate ◽  
Photostimulable Phosphor Plate ◽  
Sensitivity Specificity

Background. Dental caries is the most important reason for tooth loss. Clinical examination is the most commonly used technique for occlusal caries diagnosis. The diagnostic power of digital systems is a matter of controversy in this field. The present study aimed to determine the diagnostic accuracy of two photostimulable phosphor plate (PSP) systems for early occlusal dentin caries in vitro. Methods. Sixty-nine extracted molar and premolar teeth were used in this study. The teeth were mounted in triple blocks, and standard radiographs were taken by the Digora and Acteon digital radiographic systems. The original and filter 1-enhanced radiographs were evaluated by two experienced observers twice at an interval of two weeks, and dentin caries was recorded in Tables prepared for the study. The teeth were then sectioned in a buccolingual direction and evaluated under a stereomicroscope. The observers’ reports were compared with microscopic findings as the gold standard. SPSS 23 was used to calculate the kappa coefficient, sensitivity, specificity, and area under the ROC curve (AUC). Statistical significance was set at P<0.05. Results. The internal and the external agreements in both imaging systems were good to excellent. The means of sensitivity, specificity, and AUC in the Acteon system were 34.1, 92.9, and 0.674, with 30.8, 94.8, and 0.659, respectively, in the Digora system. Conclusion. The accuracy of early occlusal caries diagnosis was poor on both systems, and no significant difference was observed between the two systems at a 95% confidence interval. Although the AUC was slightly higher in the original images, there was no significant difference between them; however, due to their high specificity, they can prevent unnecessary treatments in the clinic.

scholarly journals Investigating the Effect of Different Metal Sheets on Reducing Radiation Behind Intraoral Digital Phosphor Plates During Intraoral Radiography using Gray Value

2020 ◽  
Vol 14 (1) ◽  
pp. 704-710
Author(s):  
Mojdeh Mehdizadeh ◽  
Mohammad Bagher Tavakoli ◽  
Farida Ghazanfari Moghaddam
Keyword(s):  
Stainless Steel ◽  
Test Procedure ◽  
Significant Difference ◽  
Metal Sheets ◽  
Photostimulable Phosphor ◽  
Phosphor Plate ◽  
The Mean ◽  
Photostimulable Phosphor Plate ◽  
Phosphor Plates ◽  
Gray Values

Purpose: To assess the efficacy of metal sheets with different thicknesses on reducing the radiation behind the photostimulable phosphor plate (PSP) during intraoral periapical examinations. Materials and Methods: In this study, a phantom head was used to investigate the effect of using metal sheets of aluminum, stainless steel, and copper with three thicknesses of 0.5, 0.75 and 1mm on reducing the radiation behind PSP. Images were acquired at 66 kVp 8 mA, and 0.16 s using the long-cone paralleling technique. The images were saved and exported to DICOM format without any post-processing. Then, they were transferred to an image processing software (Mimics, Materialize, Leuven, Belgium), and their mean gray values (MGV) were determined. The acquired data were statically analyzed for accessing the significant difference in groups means using ANOVA, and the means of groups were compared using Tukey’s test procedure. Results: The MGV of the second PSP differed significantly from those PSPs slotted with various metals (p<0.001). The MGV for lead and stainless steel at 0.5 mm thickness was insignificant (p = 0.852), but differed significantly from other metals. The MGV for PSPs with 0.75 mm stainless steel and 1 mm copper slots were varied significantly from those of all other PSP and metals. The 1 mm copper group was significantly different from all other groups (p<0.001). Conclusion: Using the mean gray value of the PSP, the use of various metal sheets of different thicknesses could effectively reduce the radiation behind the PSPs during periapical radiographs.

scholarly journals Occlusal caries diagnosis in permanent teeth: an in vitro study

2005 ◽  
Vol 19 (4) ◽  
pp. 243-248 ◽  
Author(s):  
Gisele Angnes ◽  
Vivian Angnes ◽  
Rosa Helena Miranda Grande ◽  
Márcio Battistella ◽  
Alessandro Dourado Loguercio ◽  
...  
Keyword(s):  
Visual System ◽  
Roc Curve ◽  
In Vitro Study ◽  
Laser Fluorescence ◽  
Permanent Teeth ◽  
Occlusal Caries ◽  
Caries Diagnosis ◽  
Occlusal Surfaces ◽  
Sensitivity Specificity

The reduction in caries prevalence has not occurred uniformly for all dental surfaces. As the occlusal surfaces are still the most likely sites for the development of lesions, new methods of diagnosis are still being evaluated. This study compared a laser fluorescence (LF) system (DIAGNOdent) with the Ekstrand’s visual system for in vitro detection of occlusal caries. A total of 57 extracted molars with macroscopically intact occlusal surfaces were selected. Two-examiners assessed 110 sites by visual inspection (VI) and LF. After ten days from the first measurement, all teeth were re-evaluated through the same methods by each examiner. Caries extension was histologically assessed (X 40). The methods were compared by means of sensitivity, specificity, intra- and inter-examiner reproducibility and area under the ROC curve. The kappa’s test showed good intra- and inter-examiner reproducibility for both methods. VI and LF showed similar sensitivities for both examiners, however, VI showed higher specificities than LF. The overall analysis, as demonstrated by the area under the ROC curve, showed that VI had a better performance than the LF device. It was concluded that the Ekstrand’s visual system is more reliable than the LF device. LF should be considered only as an adjuvant for occlusal caries diagnosis.

scholarly journals Introducing intrauterine antibiotic infusion as a novel approach in effectively treating chronic endometritis and restoring reproductive dynamics: a randomized pilot study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Konstantinos Pantos ◽  
Mara Simopoulou ◽  
Evangelos Maziotis ◽  
Anna Rapani ◽  
Sokratis Grigoriadis ◽  
...  
Keyword(s):  
Fertility Restoration ◽  
Statistical Significance ◽  
Antibiotic Administration ◽  
Oral Antibiotic ◽  
Treatment Rate ◽  
Chronic Endometritis ◽  
Vitro Fertilization ◽  
Significant Difference ◽  
Antibiotic Infusion

AbstractThe chronic nature of Chronic Endometritis (CE) along with the challenging management and infertility entailed, call for cutting-edge therapeutic approaches. This study introduces the novel treatment of intrauterine antibiotic infusion (IAI) combined with oral antibiotic administration (OAA), and it assesses respective performance against the gold standard treatment of OAA. Data sourced herein reports on treatment efficiency and fertility restoration for both patients aiming to conceive naturally or via In Vitro fertilization. Eighty CE patients, 40 presenting with recurrent implantation failure, and 40 with recurrent pregnancy loss, were enrolled in the IVF and the natural conception arm respectively. Treatment was subjected to randomization. Effectively treated patients proceeded with either a single IVF cycle or were invited to conceive naturally over a 6-month period. Combination of IAI and OAA provided a statistically significant enhanced effectiveness treatment rate (RR 1.40; 95%CI 1.07–1.82; p = 0.01). No statistically significant difference was observed regarding the side-effects rate (RR 1.33; 95%CI 0.80–2.22; p = 0.52). No statistically significant difference was observed for either arm regarding live-birth rate. Following an intention-to-treat analysis, employment of IAI corresponds to improved clinical pregnancy rate-albeit not reaching statistical significance. In conclusion, complimentary implementation of IAI could provide a statistically significant enhanced clinical treatment outcome.

scholarly journals Performance of the BD FACSPresto near to patient Analyzer in comparison with representative conventional CD4 instruments in Cameroon.

2020 ◽  
Author(s):  
Bertrand Sagnia ◽  
Fabrice MBAKOP Ghomsi ◽  
Ana Gutierrez ◽  
Samuel SOSSO ◽  
Rachel KAMGAING ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Statistical Significance ◽  
P Value ◽  
Resource Constrained ◽  
Inform Consent ◽  
Remote Areas ◽  
Significant Difference ◽  
Sensitivity Specificity ◽  
Cd4 Testing

Abstract Background In the context of scaling the viral load in resource limited settings, following HIV infected patient’s adults and children with CD4+ T-lymphocyte count still very important in settings where the decentralization of treatment still has some challenges. Effective HIV monitoring in these resource-constrained settings needs affordable and reliable CD4+ T lymphocytes enumeration methods. We investigated the validity of a BD FACSPresto POC which is a dedicated system for enumeration that uses immunofluorescent technologies. In this study, we have assessed the sensitivity, specificity and correlation between most representative flow cytometry instruments present in Cameroon with more than 5000 CD4 T cells tests per year including FACSCalibur, FACSCount, and PIMA POC from Becton dinkinson and ALERE respectively. Methods 268 patients aged from 1 to 72 years old were enrolled and included in the study after inform consent. The BD FACSPresto POC CD4+ T cell technology was placed at CIRCB and operated by technician staff. HIV infected patients were from Chantal BIYA international reference Center (CIRCB), Centre de Sante Catholique de NKOLODOM, Centre de Sante Catholique de BIKOP and CASS de Nkolndongo – Yaounde We compared the accuracy of the BD FACSPresto and three existing reference technologies with more than 5000 tests per year like FACSCalibur, FACSCount and PIMA according to the number of CD4 test done per year and their repartition in the country. Bland – Altman method and correlation analysis were used to estimate mean bias and 95% limits of agreement and to compare the methods, including analysis by subgroup of participant gestational age. In addition sensitivity and specificity were determined. Statistical significance was set at p-value < 0.05 Results The BD FACSPresto POC system has excellent precision, accuracy and linearity for CD4+ T lymphocytes enumeration. Good correlations were obtained between the BD FACSPresto poc system and other single platform methods. Bland–Altman plots showed interchangeability between two machines mean bias BD-FACSPresto vs PIMA= -126,522(-161,221 to -91,822) BD-FACSPresto vs FACSCount= -38,708 (-58,935 to -18,482) and FACSPresto vs FACSCALIBUR= 0,791(-11,908 to 13,491). Mean difference with Absolute CD4+ T-lymphocyte values obtained from the BD FACSPresto system correlated well with PIMA, FACSCount, and FACSCalibur method with R 2 equal to 0.88, 0.92 and 0.968 respectively with P < 0.001 for all. The mean comparison between values obtained from BD FACSPresto with PIMA, FACSCount, and FACSCalibur using paired T test give P=0.17, P=0.5 and P=0.6 respectively meaning that there is no significant differences between values obtained with BD FACSPresto and PIMA, FACSCount or FACSCalibur CD4 enumeration machines. Further analysis revealed close agreement between all the three instruments with no significant difference between the forth methods (P=0.91) Conclusion This BD-FACSPresto POC system is a simple, robust and reliable system for enumeration of absolute and percentage of CD4+ T-lymphocytes especially suitable for remote areas with limited resources. Having one BD-FACSPresto POC system easy to use, should reduce the cost and thus increase and improved access to CD4 testing for HIV infected patients in resource-constrained countries. BD-FACSPresto POC CD4 will enable reduction in patient time and improve the overall quality of ART service count and may improve test access in remote areas. This technology can allow for greater decentralization and wider access to CD4 testing and ART

scholarly journals Microleakage analysis of dental caries lesions sealed with flow resin and compared to microhybrid resin restorations in dentin

2018 ◽  
Vol 66 (2) ◽  
pp. 141-146
Author(s):  
Amanda de Albuquerque VASCONCELOS ◽  
Juliana Tietbohl de Almeida REIS ◽  
Bianca Fiorentin MOURA ◽  
Daniela Cavalcante GIRÃO ◽  
José Carlos Pettorossi IMPARATO ◽  
...  
Keyword(s):  
Composite Resin ◽  
Descriptive Analysis ◽  
Resin Flow ◽  
Occlusal Caries ◽  
Dye Penetration ◽  
Group I ◽  
Significant Difference ◽  
Distal Direction ◽  
Caries Lesions

ABSTRACT Objective: To evaluate the sealing of cavities of dentinal occlusal caries lesions, reproduced in vitro, with flow resin compared to cavity restorations presenting healthy dentin using microhybrid composite resin. Methods: The sample consisted of 27 healthy deciduous molars where cavities of approximately 2 mm in the fossa region were performed and occlusal cleft of each tooth were sealed, impermeabilization was performed and the sample was randomly divided into 2 groups: group I underwent cariogenic challenge and occlusal sealing with resin flow. The teeth of group II were restored with microhybrid composite resin. The teeth were immersed in 5% methylene blue for 8 hours at 37° C and washed until all the dye was removed from the surface. The teeth were sectioned in the mesio-distal direction. The penetration of the dye was evaluated: 0- no penetration; 1- dye penetration up to 1/3 of the restoration; 2- dye penetration up to 2/3 of the restoration depth; 3 - penetration of dye into the pulp wall. The results were analyzed by the Biostat 4.0 program. Descriptive analysis and the mode among the examiners submitted to the Mann-Whitney test. Results: There was no significant difference in microleakage between restoration performed in healthy dentin with microhybrid composite resin or maintenance of infected dentin in primary teeth sealed with resin flow (p = 0.6035). Conclusion: It was concluded that the marginal infiltration of primary molars sealed with microhybrid composite resin and resin flow was not influenced by the removal -or not -of the carious tissue or the material used.

CEP701 and PKC412 Predictably and Reliably Inhibit FLT3 Phosphorylation in Primary AML Blasts but Their Induction of a Cytotoxic Response Appears to Be Much More Variable.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 95-95 ◽  
Author(s):  
Steven Knapper ◽  
Alan K. Burnett ◽  
Amanda F. Gilkes ◽  
Kenneth I. Mills ◽  
Val Walsh
Keyword(s):  
Statistical Significance ◽  
Annexin V ◽  
Expression Level ◽  
Wild Type ◽  
Response Curves ◽  
Allele Ratio ◽  
Cytotoxic Response ◽  
Significant Difference ◽  
Flt3 Expression

Abstract Activating mutations of the receptor tyrosine kinase FLT3 are present in approximately one-third of AML cases and are associated with an adverse prognosis. FLT3 is expressed in over 90% of cases of AML and many non-mutants show evidence of FLT3 activation, which may play a significant signalling role in leukaemogenesis, making FLT3 an attractive therapeutic target. CEP701 (Cephalon) and PKC412 (Novartis) are orally-bioavailable indolocarbazole derivatives that potently inhibit FLT3 phosphorylation. We studied the relationship between in vitro inhibition of FLT3 phosphorylation and induction of cytotoxicity in primary AML blasts from 12 patients. 7 of the cases were FLT3 mutants (6 ITDs and 1 D835 point mutant), the amount of mutant RNA varying between 7% and 84% of total FLT3 RNA expressed. The blasts were exposed for 1 hour to a range of concentrations of CEP701 and PKC412, lysed and immunoprecipitated with an anti-FLT3 antibody. After sequential immunoblotting with anti-phosphotyrosine and anti-FLT3 antibodies, inhibition of FLT3 phosphorylation was measured by densitometry. Both drugs inhibited FLT3 phosphorylation in all samples with lower concentrations required in FLT3 mutants. CEP701 inhibited FLT3 phosphorylation with median IC50s of 3.7nM and 11.9nM in mutant and wild type (WT) cases respectively (p=0.0006). IC50s for PKC412 were 7.7nM and 59.8nM in mutant and WT cases (p=0.0268). Induction of cytotoxicity was assessed by MTS assay following 72-hour exposure of blasts to a range of concentrations of CEP701 and PKC412. Cytotoxic responses to both drugs were greater in FLT3 mutants than WT cases at each dose studied and in terms of IC50 dose (median IC50s in mutant and WT cases: 95nM and 231nM with CEP701, 1.24 μM and 1.61μM with PKC412) although these differences did not reach statistical significance. Annexin V binding apoptosis assay produced similar dose response curves. Both agents showed greater inter-case variability in cytotoxic response than in sensitivity to inhibition of FLT3 phosphorylation. A lack of cytotoxic response to FLT3 inhibition with CEP701 was seen in the ITD mutant with the lowest ratio of mutant to WT FLT3 RNA (0.08) and several WT samples displayed resistance to in vitro induction of cytotoxicity despite almost complete inhibition of FLT3. Induction of cytotoxicity with PKC412 in both mutant and WT cases generally required doses well in excess of those required to fully inhibit FLT3 phosphorylation. Cases were further stratified by flow cytometric measurement of surface FLT3 expression, and by immunoblotting to measure STAT5 dephosphorylation in response to both drugs. No significant difference in overall FLT3 expression was seen between mutant and WT cases. Interestingly the highest FLT3 expression level was seen in a wild type case that was highly sensitive to CEP701. Inhibition of STAT5 phosphorylation appeared closely linked to FLT3 inhibition, although in some cases a good cytotoxic response was achieved despite failure to inhibit STAT5, suggesting involvment of other signalling pathways. In summary, although both CEP701 and PKC412 predictably and reliably inhibit FLT3 phosphorylation in primary AML blasts, their induction of cytotoxicity appears to be much more variable. A number of factors may influence this including variations in level of dependency on FLT3 signalling for blast survival, mutant to WT allele ratio and overall FLT3 expression level. Effects on targets other than FLT3 also need to be considered.

Benefits of using a photostimulable phosphor plate protective device

2021 ◽  
pp. 20200339
Author(s):  
Graziela de Moura ◽  
Mariana Boessio Vizzotto ◽  
Priscila Fernanda da Silveira Tiecher ◽  
Nádia Assein Arús ◽  
Heraldo Luis Dias da Silveira
Keyword(s):  
Image Quality ◽  
Compressive Load ◽  
Control Group ◽  
Protective Device ◽  
Photostimulable Phosphor ◽  
Plate Size ◽  
Phosphor Plate ◽  
Photostimulable Phosphor Plate ◽  
Post Hoc ◽  
Gray Values

Objectives: To develop and test a protective device (PD) to increase the resistance of photostimulable storage phosphor (PSP) plate to compressive load, and assess the resulting image quality. Methods: Two prototypes, polyvinylchloride sheets of 0.3 mm and 0.7 mm each, were developed for PSP plate size 2. The resistance to compressive load was tested using eight new PSPs divided into four test groups: (1) PSP, (2) PSP and paperboard protector, (3) PSP and 0.3 mm PD, and (4) PSP and 0.7 mm PD. The resulting images were analyzed by three oral radiologists, based on the consensus for image artifacts. Additionally, the objective image quality test was performed with four new PSPs, using an 8-step wedge aluminum scale. The mean gray values and standard deviation were measured in a total of 240 images, and the data were analyzed using analysis of variance with Bonferroni post-hoc test. Results: Artifacts were seen in the PSP control group starting at 40 n, and at 150 n, 175 n and 300 n in 0.3 mm PD, paperboard protector and 0.7 mm PD, respectively. Although there was no statistical difference among groups, there were differences between exposure times (0.06–0.25 s, 0.06–0.40 s, and 0.10–0.40 s). Scanning resolution of 20 lp/mm showed higher mean gray value than 25 and 40 lp/mm (p < 0.05) Conclusion: The developed PDs improved the PSP resistance to compressive forces, with low interference on the pixel gray values, regardless of exposure time and spatial resolution. Nevertheless, the 0.7 mm PD could withstand the maximum compressive load.

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