ENHANCEMENT OF CAFFEINE RECOVERY FROM ROASTED COFFEE POWDER BY ENZYME CELLULASE PRETREATMENT, HOT-WATER EXTRACTION AND CO2-ADDING
The efficiency of caffeine recovery during extraction with highpressure hot-water solvent was investigated to find out the conditions for maximum caffeine recovery in roasted coffee powder. In addition, to support the extraction process with high-pressure hot-water solvent, cellulase enzyme pretreatment of roasted coffee powder is also applied and has demonstrated the supporting role of the enzyme in improving caffeine extraction efficiency. CO2 can participate in the extraction process with the role of changing the pH of the solvent at the high temperature and obtained positive results. In the presence of the enzyme cellulase at avalue of 8 UI/g, the pretreatment time was 90 minutes; the extraction process performed at 110oC for temperature and 10 min for the extraction time, the efficiency of caffeine recovery can reach 96,36 ±2,56%.