Microwave Assisted Multi-Stage Countercurrent Extraction of Dihydromyricetin from Ampelopsis grossedentataa

2011 ◽  
Vol 7 (4) ◽  
Author(s):  
Wei Li ◽  
Cheng Zheng ◽  
Jian Zhao ◽  
Zhengxiang Ning
Keyword(s):  
Extraction Efficiency ◽  
Extraction Process ◽  
Extraction Time ◽  
Countercurrent Extraction ◽  
Bioactive Substances ◽  
Extraction Solvent ◽  
Microwave Assisted ◽  
Multi Stage ◽  
The Matrix ◽  
Substantial Concentration

A novel microwave assisted multi-stage countercurrent extraction (MAMCE) technique was developed for the extraction of dihydromyricetin from Chinese rattan tea, Ampelopsis grossedentata. The technique combined the advantages of microwave heating and dynamic multi-stage countercurrent extraction and achieved marked improvement in extraction efficiency over microwave assisted batch extraction. Analysis of dihydromyricetin concentrations in the solvent and matrix throughout the extraction process showed that by dividing the extraction into multiple stages and exchanging of solvents between stages, steady and substantial concentration gradients were established between the matrix and solvent, thus enabling the achievement of high extraction efficiency. The yield of dihydromyricetin was significantly affected by temperature, pH, solvent/material ratio and extraction time, and optimal extraction conditions were found to be 80-100°C, at acidic pH with a solvent/material ratio of 25-30 to 1 and extraction time of 5-10 min. With the high extraction efficiency and low usage of extraction solvent, MAMCE could prove to be a promising extraction technique which can be applied to the extraction of dihydromyricentin and other bioactive substances from natural materials.

scholarly journals Microwave-Assisted Industrial Scale Cannabis Extraction

Technologies ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 45
Author(s):  
Marilena Radoiu ◽  
Harmandeep Kaur ◽  
Anna Bakowska-Barczak ◽  
Steven Splinter
Keyword(s):  
Continuous Flow ◽  
Extraction Efficiency ◽  
Scale Up ◽  
Extraction Process ◽  
Extraction Methods ◽  
Flowering Plant ◽  
Industrial Scale ◽  
Microwave Assisted ◽  
Operation Conditions ◽  
Assisted Extraction

Cannabis is a flowering plant that has long been used for medicinal, therapeutic, and recreational purposes. Cannabis contains more than 500 different compounds, including a unique class of terpeno-phenolic compounds known as cannabinoids. Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) are the most extensively studied cannabinoids. They have been associated with the therapeutic and medicinal properties of the cannabis plant and also with its popularity as a recreational drug. In this paper, an industrial method for cannabis extraction using 915 MHz microwaves coupled with continuous flow operation is presented. The main advantages of the microwave-assisted extraction (MAE) are associated to the continuous-flow operation at atmospheric pressure which allows for higher volumes of biomass to be processed in less time than existing extraction methods, with improved extraction efficiency leading to increased final product yields, improved extract consistency and quality because the process does not require stopping and restarting material flows, and ease of scale-up to industrial scale without the use of pressurised batch vessels. Moreover, due to the flexibility of changing the operation conditions, MAE eliminates additional steps required in most extraction methods, such as biomass decarboxylation or winterisation, which typically adds at least a half day to the extraction process. Another factor that sets MAE apart is the ability to achieve high extraction efficiency, i.e., up to 95% of the active compounds from cannabis biomass can be recovered at industrial scale.

scholarly journals Process optimization of supercritical carbon dioxide (SC-CO2) extraction parameters for extraction of deoxynojirimycin (1-DNJ) from mulberry (Morus alba L.) leaves

2016 ◽  
Vol 8 (1) ◽  
pp. 405-411 ◽  
Author(s):  
V. Ramya ◽  
Udaykumar Nidoni ◽  
Sharangouda Hiregoudar ◽  
C. T. Ramachandra ◽  
J. Ashoka ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Extraction Efficiency ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Solvent ◽  
Mulberry Leaf ◽  
Leaf Powder ◽  
Dynamic Extraction ◽  
Extraction Parameters ◽  
Carbon Dioxide Co2

In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1-DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosolvent, and extraction parameters such as pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) anddynamic extraction time (40, 60 and 80 min) were systematically investigated by full factorial design to obtain the optimum extraction efficiency and extraction yield. Under optimized conditions (pressure of 200 bar, temperature of 50 °C and dynamic extraction time of 80 min), DNJ enriched extract was obtained with high extraction efficiency (96.46 %) and extraction yield (13.41 %), enabling this product to use for nutraceutical purpose. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.

scholarly journals Ultrasonic Extraction of Tropane Alkaloids from Radix physochlainae Using as Extractant an Ionic Liquid with Similar Structure

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2897 ◽  
Author(s):  
Alula Yohannes ◽  
Baohui Zhang ◽  
Bing Dong ◽  
Shun Yao
Keyword(s):  
Aqueous Solution ◽  
Ionic Liquid ◽  
Extraction Efficiency ◽  
Tropane Alkaloids ◽  
Raw Material ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Mechanism ◽  
Extraction Solvent ◽  
Solid Liquid

In this research, tropane alkaloids in Radix physochlainae were extracted by tropine-type ionic liquid (IL) aqueous solutions under ultrasound assistance, and N-propyltropine hexafluorophosphate ([C3Tr][PF6]) was found to be the most ideal IL in this extraction mode after comprehensive screening. When 0.03 mol/L [C3Tr][PF6] aqueous solution was chosen as the extraction solvent, the solid-liquid ratio of raw material powders and ionic liquid aqueous solution was 1:20 (g/mL), ultrasonic power was 90 W and extraction time was 30 min, the extraction efficiency of tropane alkaloids has reached 121.3%. Compared with common heating extraction, it can further shorten the extraction time, improve extraction efficiency and decrease IL consumption. Furthermore, extraction mechanism together with potential toxicity of IL have been explored and discussed.

Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

2021 ◽  
Vol 19 (1) ◽  
pp. 830-842
Author(s):  
Quoc Toan Tran ◽  
The Dan Pham ◽  
Thanh Duong Nguyen ◽  
Van Huyen Luu ◽  
Huu Nghi Do ◽  
...  
Keyword(s):  
Large Scale ◽  
Extraction Efficiency ◽  
Raw Materials ◽  
Biological Activities ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Hep G2 ◽  
Independent Parameters

Abstract Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa and optimized this process using response surface methodology (RSM) according to a central composite design, with three independent parameters including extraction time (min), extraction temperature (°C), and solvent/material ratio (mL/g). Two dependent variables were the Mu-A content (mg/g raw materials) and extraction efficiency (%). The optimal conditions to extract Mu-A were found to be as follows: extraction temperature, 67°C; extraction time, 165 min; and solvent/material ratio, 5:1. Under these conditions, the Mu-A content and extraction efficiency were 38.94 ± 1.31 mg/g raw materials and 34.98 ± 1.18%, respectively. Mu-A exhibited antiproliferation and antitumor-promoting activity against the HepG-2 cell line. The present optimization work of Mu-A extraction from G. stenocarpa roots contributed to the attempt of designing a large-scale extraction process for the compound and further exploitation of its potential in vivo applications.

scholarly journals Optimization of Microwave-Assisted Extraction Process of Callicarpa candicans (Burm. f.) Hochr Essential Oil and Its Inhibitory Properties against Some Bacteria and Cancer Cell Lines

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 173 ◽  
Author(s):  
Quoc Toan Tran ◽  
Thu Le Vu Thi ◽  
Tien Lam Do ◽  
Hong Minh Pham Thi ◽  
Bich Hoang Thi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Cell Lines ◽  
Cancer Cell ◽  
Microwave Power ◽  
Cancer Cell Lines ◽  
Extraction Process ◽  
Raw Material ◽  
Extraction Time ◽  
Microwave Assisted ◽  
Wide Range

Callicarpa candicans (Burm. f.) Hochr. (Callicarpa cana L.) is a medicinal plant that is distributed mainly in the tropics and subtropics of Asia and finds a wide range of uses in traditional medicine. In this study, we attempted and optimized the microwave-assisted hydro-distillation (MAHD) process to obtain essential oil from the leaves of C. candicans. In addition, the obtained oil was analyzed for volatile composition by gas chromatography–mass spectrometry (GC-MS) and assayed for bioactivity against several bacteria and cancer cell lines. To optimize the extraction process, response surface methodology (RSM) in combination with central composite design (CCD) was adopted. Experimental design and optimization were carried out with respect to three experimental factors including the ratio of water to raw material, extraction time, and microwave power. The optimal extraction conditions were obtained as follows: water to raw material ratio of 6/1 (v/w), extraction time 42 min, and microwave power 440 W. Composition determination of the obtained C. candicans essential oil indicated the presence of predominant components including caryophyllene <b-> (10.45%), cadinene <d-> (10.28%), gurjunene <a-> (8.95%), muurolene <g-> (8.92%), selinene <a-> (7.06%), selinene <b-> (5.59%), and copaene <a-> (5.40%). In comparison with the essential oils obtained via traditional hydro-distillation method, the essential oil extracted by MAHD exhibited superior anti-proliferative activity on all tested cancer cell lines. Current results imply that the MAHD is capable of recovering biologically-active natural products of greater quantity than that recovered by the conventional distillation.

Optimization of Extraction Process of Lycopene from Watermelon (Citrullus lanatus) by Response Surface Methodology

2011 ◽  
Vol 140 ◽  
pp. 385-393 ◽  
Author(s):  
Xian Feng Shi ◽  
Yuan Xu ◽  
Yu Hua Li ◽  
Hong Xia Zeng ◽  
Yu Hong Sun
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Citrullus Lanatus ◽  
Extraction Process ◽  
Quadratic Equation ◽  
Raw Material ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Extraction Solvent

The extraction processing of lycopene from watermelon was investigated in this paper. Based on single factor experiments, response surface methodology (RSM) was adopted to study the key parameters such as number of extraction, extraction temperature, extraction time in order to increase extraction rate of lycopene. A second order quadratic equation was established and the applicability of model and interaction involved factors on predicting the lycopene extracting content was verified. The results indicated that the optimum extraction conditions were hexane (containing 2% dichloromethane) as extraction solvent, ratio of solvent to raw material of 3:1 (mL/g), number of extraction of 2, extraction time of 1.9 h and extraction temperature of 29.8°C. Under these conditions the extracting content of lycopene was 14.71±0.22 mg/kg, which is well in agreement with value predicted by the model.

scholarly journals Studies on Effect of Temperature on Asphaltene precipitation using Multi Stage Extraction

2017 ◽  
Vol 7 (1.1) ◽  
pp. 599
Author(s):  
Kethineni Chandrika ◽  
N Sampath ◽  
Jobin V J ◽  
E Seetha Ramaiah ◽  
Vishnu Vipin ◽  
...  
Keyword(s):  
Good Yield ◽  
Maximum Yield ◽  
Extraction Process ◽  
Extraction Time ◽  
Effect Of Temperature ◽  
Solvent Ratio ◽  
Multistage Process ◽  
Asphaltene Precipitation ◽  
Extraction Percentage ◽  
Multi Stage

In the case of asphaltene extraction, single stage extraction given a maximum yield at 30°c with the sample to solvent ratio of 1:25. The time taken for the extraction process is 24 hours. In the case of long step extraction process its sample to ratio is 1:4. When the temperature increased in the process the yield also started increasing. The maximum yield we could get in the temperature range of 30°c to 50°c. It is possible to reduce the extraction time by increasing the temperature and it gives a good yield of asphaltene with the usage of multistage process. It increased the asphaltene presence about 69% in the solvent. The asphaltene yield at 30°c give raise an extraction of 85% in a six-stage extraction process. It is possible to get the same extraction percentage in a temperature of 50°c with three stage extraction process.  

scholarly journals Computational-Based Study of QuEChERS Extraction of Cyclohexanedione Herbicide Residues in Soil by Chemometric Modeling

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2009 ◽  
Author(s):  
Juan Villaverde ◽  
Beatriz Sevilla-Morán ◽  
Carmen López-Goti ◽  
José Alonso-Prados ◽  
Pilar Sandín-España
Keyword(s):  
Solid Phase ◽  
Extraction Process ◽  
Factorial Experimental Design ◽  
Independent Variables ◽  
Extraction Solvent ◽  
The Matrix ◽  
Process Behavior ◽  
Face Centered ◽  
Soil Weight ◽  
Solvent Volume

Assessment of two buffered QuEChERS (quick, easy, cheap, effective, rugged, and safe) versions (i.e., citrate and acetate) modified by including methanol to recover the residues of three cyclohexanedione oxime (CHD) herbicides and three of their byproducts from agricultural soil was performed. In this context, a full second-order face-centered factorial experimental design was developed to quantify the influences of the main five variables (i.e., extraction time, water content, soil weight, and extraction solvent volume and composition) on the target compound recoveries. The fitting equations satisfactorily described the extraction process behavior. The mathematical models also showed the most influencing independent variables (i.e., extraction solvent composition and soil weight). Handling simpler expressions was possible with the acetate QuEChERS but not with the citrate QuEChERS. The recoveries of the CHD residues were close to 100% after performing the extraction under suitable conditions. Furthermore, dispersive solid-phase extraction (dSPE) clean-up steps were assessed to reduce the matrix effect in mass spectrometry. In this sense, the citrate QuEChERS in combination with the PSA + C18 clean-up step was the best option for the extraction of CHD residues.

scholarly journals The Effects of Parameters on the Efficiency of DLLME in Extracting of PAHs from Vegetable Samples

2018 ◽  
Vol 7 (4.35) ◽  
pp. 15 ◽  
Author(s):  
Yeong Hwang Tan ◽  
Mee Kin Chai ◽  
Ling Shing Wong
Keyword(s):  
Microwave Power ◽  
Extraction Efficiency ◽  
Irradiation Time ◽  
Extraction Time ◽  
Extraction Solvent ◽  
Vegetable Samples ◽  
Low Viscosity ◽  
Vegetable Sample ◽  
Significant Difference ◽  
Extraction Solvents

An effective analytical method based on microwave-assisted extraction (MAE) and dispersive liquid-liquid microextraction (DLLME) followed by gas chromatography-flame ionization detector (GC-FID) was developed for the determination of polycyclic aromatic hydrocarbons (PAHs) in vegetable samples. In most cases, the details of the parameters influencing the efficiency of DLLME in extraction are not well studied. Understanding the reactions of solvents in extraction is the important task on selecting of an appropriate solvent in the process. The effects of parameters affecting the extraction efficiency of DLLME, including extraction solvent and dispersive solvent, extraction time and MAE, such as solvent, microwave power and irradiation time, were studied and explained. The impacts of physiochemical properties of the selected extraction solvents on the extraction efficiency were also investigated. The results indicated that extraction solvents with low viscosity and low polarity have better extraction efficiency in extraction of PAHs from vegetable sample. No significant difference was observed for the effects of selected dispersive solvents and extraction time on extraction efficiency. In MAE, the types of solvent, microwave power and irradiation time implied some critical effects on the extraction efficiency of DLLME.

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