Synthesis and characterization of silver nanoparticles using as a reducing agent plant extract of Dandelion (Taraxacun officianale)

The objective of this work was to synthesize the synthesis of silver nanoparticles using as a reducing agent of ionic silver the plant extract of Dandelion (Taraxacun officianale), vegetable of high availability and low cost, as an alternative to the processes conventional, based on the antioxidant capacity of plant extracts that reduce metals in solution. The nanoparticles prepared by this method were characterized by the golden yellow color characteristic of silver nanoparticle solutions. Measurements with UV-Vis spectroscopy of aqueous solutions of Ag1 + ions after coming into contact with plant extracts of Dandelion at different pHs showed an intense absorption band around 400-450 nm, characteristic of the resonance of the Plasmon of silver nanoparticles. Through the scanning of the samples by means of AFM (atomic force microscopy), morphological information of the nanoparticles is obtained, from 3D topographic images of them, such as distribution, size and shape of the silver nanoparticles. Finally, its antibacterial activity was tested against the Escherichia coli strain.

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Synthesis of silver nanoparticles from extracts of Scytonema geitleri HKAR-12 and their in vitro antibacterial and antitumor potentials

Letters in Applied NanoBioScience ◽

10.33263/lianbs83.576585 ◽

2019 ◽

Vol 8 (3) ◽

pp. 576-585

Keyword(s):

Silver Nanoparticles ◽

Coli Strain ◽

X Ray Diffraction ◽

Xrd Pattern ◽

E Coli ◽

Vis Spectroscopy ◽

Shape Structure ◽

Actual Size

In the present study silver nanoparticles (AgNPs) have been synthesized through the cell-free extracts of the rooftop dwelling cyanobacterium Scytonema geitleri HKAR-12. UV-VIS spectroscopy, FTIR, X-ray diffraction, SEM and TEM were used for the determination of morphological, structural and optical properties of synthesized AgNPs. Extracts of Scytonema geitleri HKAR-12 have the ability to reduce AgNO3 to Ag0. Sharp peak at 422 nm indicated the rapid synthesis of AgNPs. FTIR results showed the presence of different groups responsible for the reduction of AgNO3 to AgNPs. XRD pattern confirmed the crystalline nature of AgNPs. SEM showed the bead shape structure of AgNPs. TEM confirmed the actual size of AgNPs to be ranging between 9-17 nm. AgNPs showed antibacterial activity against Pseudomonas aeruginosa, Escherichia coli strain1 and E. coli strain 2 and 11 μg/mL of AgNPs effectively inhibited the growth of MCF-7 cells. Hence, Scytonema geitleri HKAR-12, isolated from the rooftop could serve as a desirable biological candidate for convenient and cheap production of AgNPs having antimicrobial and anti-cancerous properties.

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Stabilization of Silver Nanoparticles by Cationic Aminoethyl Methacrylate Copolymers in Aqueous Media—Effects of Component Ratios and Molar Masses of Copolymers

Polymers ◽

10.3390/polym11101647 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1647 ◽

Cited By ~ 1

Author(s):

Mikhailova ◽

Senchukova ◽

Lezov ◽

Gubarev ◽

Trützschler ◽

...

Keyword(s):

Silver Nanoparticles ◽

Analytical Ultracentrifugation ◽

Silver Salt ◽

Aqueous Media ◽

Reducing Agent ◽

Methacrylate Copolymers ◽

Vis Spectroscopy ◽

Cationic Copolymers ◽

Consistent Information ◽

Scanning Electron

The ability of aminoethyl methacrylate cationic copolymers to stabilize silver nanoparticles in water was investigated. Sodium borohydride (NaBH4) was employed as a reducing agent for the preparation of silver nanoparticles. The objects were studied by ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), analytical ultracentrifugation (AUC) and scanning electron microscopy (SEM). Formation of nanoparticles in different conditions was investigated by varying ratios between components (silver salt, reducing agent and polymer) and molar masses of copolymers. As a result, we were successful in obtaining nanoparticles with a relatively narrow size distribution that were stable for more than six months. Consistent information on nanoparticle size was obtained. The holding capacity of the copolymer was studied.

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Phytogenic Synthesis of Nano Silver from Madagascar Periwinkle Extracts and Their Angiogenic Activities in Zebrafish Embryos (ZFE)

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2020.3073 ◽

2020 ◽

Vol 12 (1) ◽

pp. 79-87

Author(s):

Govindarajan Kathiravan ◽

Kosuru Rekha Yamini ◽

Kalyanaraman Rajagopal ◽

Sambandam Anandan ◽

Kwang Jin Kim ◽

...

Keyword(s):

Silver Nanoparticles ◽

Plant Extracts ◽

Visible Spectrum ◽

Inhibitory Effect ◽

Zebrafish Embryos ◽

Fragmentation Pattern ◽

Madagascar Periwinkle ◽

Plasmon Resonance Peak ◽

Pathological Conditions ◽

Vis Spectroscopy

Green biosynthesis of silver nanoparticles utilizing plant extracts is a simple, cost effective system of generating nanoparticles in an ecofriendly environment. In the present study, biosynthesis of silver nanoparticles was carried out using different parts of Madagascar periwinkle plant extracts. The production of the silver nanoparticles (AgNPs) was detected visually by change in color of the reaction mixture from yellow to reddish brown in a 8 h treatment at 37 °C. Further, the AgNPs were analyzed by various standard characterization techniques such UV-Vis spectroscopy, Electron Microscopy (TEM and SEM), FT-IR, and GC-MS analysis. The UV-Visible spectrum showed the plasmon resonance peak for AgNPs at 435 nm, indicating the formation of silver nanoparticles. FTIR analysis showed a good interaction between the plant extract and NPs. The morphology of the NPs varied in shape has observed through TEM with the particle size less than 10 nm. The phytoformulated nanoparticles were found to be well dispersed in solution and stable due to capping molecules present in the reaction mixture. The chromatographic analysis (GC-MS) displayed a prominent fragmentation pattern especially at the higher molecular weight in the range of 280 to 271. Angiogenesis is an important physiological process that plays a crucial role in several pathological conditions (tumor growth and metastasis). The green synthesized AgNPs were assessed for their angiogenic inhibition properties using zebrafish embryos as a model system. The biogenic AgNPs treatment at the dose of 100 μl and 150 μl showed significant anti-angiogenesis (inhibitory effect on angiogenesis), which represents more effectiveness in controlling the vessel formation during pathological conditions.

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On the Influence of Silver Nanoparticles Size in the Electrical Conductivity of PEDOT: PSS

Materials Science Forum ◽

10.4028/www.scientific.net/msf.644.85 ◽

2010 ◽

Vol 644 ◽

pp. 85-90 ◽

Cited By ~ 21

Author(s):

Rebeca G. Melendez ◽

Karla J. Moreno ◽

Ivana Moggio ◽

Eduardo Arias ◽

Arturo Ponce ◽

...

Keyword(s):

Electrical Conductivity ◽

Particle Size ◽

Silver Nanoparticles ◽

Reducing Agent ◽

Molar Ratio ◽

Plasmon Band ◽

Vis Spectroscopy

In this paper, we studied the influence of the silver nanoparticles size on the electrical conductivity of PEDOT:PSS in Ag(PEDOT:PSS) films. The silver nanoparticles were synthesized in presence of PEDOT:PSS by varying the molar ratio between AgNO3 and the reducing agent (NaBH4). Both the particle size determined by TEM and the plasmon band obtained by UV-Vis spectroscopy were found to be strongly dependent on the reducing agent concentration. The electrical conductivity increases inversely with the concentration of reducing agent from 5.24 x 10-4 up to 1.63 S/cm; three orders of magnitude higher than pristine PEDOT:PSS.

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Evaluation of Larvicidal Efficacy of Ricinus communis (Castor) Plant Extract and Synthesized Green Silver Nanoparticles against Aedes albopictus

Journal of Arthropod-Borne Diseases ◽

10.18502/jad.v14i2.3734 ◽

2020 ◽

Author(s):

Muhammad Waris ◽

Shabab Nasir ◽

Azhar Rasule ◽

Iqra Yousaf

Keyword(s):

Silver Nanoparticles ◽

Aedes Albopictus ◽

Plant Extract ◽

Ricinus Communis ◽

Probit Analysis ◽

Vis Spectroscopy ◽

Percent Mortality ◽

Ft Ir ◽

Chemical Pesticides ◽

Lc50 And Lc90

Background: Aedes mosquitoes are the most important group of vectors having ability of transmitting pathogens including arboviruses that can cause serious diseases like Chikungunya fever, Dengue fever and Zika virus in human. Biosynthesis and the use of green silver nanoparticles (AgNPs) is an important step in the search of reliable and ecofriendly control of these vectors. Methods: In this study an aqueous leaves extract of Ricinus communis (castor) and silver nanoparticles (AgNPs) synthesized from this extract were evaluated as larvicidal agent for 2 nd and 3rd instar larvae of the Aedes albopictus. Different concentrations (50, 100, 150, 200 and 250ppm) of plant extract and synthesized nanoparticles were prepared and applied on second and third instar larvae. The percent mortality was noted after 6, 12, 18, 24, 30, 36, 42 and 48H of exposure and subjected to probit analysis to calculate LC50 and LC90. Results: Synthesized Ag+ nanoparticles were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and energy-dispersive X-ray spectroscopy (XRD). The nanoparticles were more toxic against larvae of Ae. albopictus with LC50 value (49.43ppm) and LC90 value (93.65ppm) for 2nd instar larvae and LC50 (84.98ppm) and LC90 (163.89ppm) for 3rd instar larvae as compared to the plant extract (149.58ppm, 268.93ppm) and (155.58ppm, 279.93ppm) for 2nd and 3rd instar larvae of Ae. albopictus respectively after 48H. Conclusion: Our results suggest the extract of R. communis and synthesized nanoparticles as excellent replacement of chemical pesticides to control the vector mosquitoes.

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Bactericidal Effect of Cotton Fabric Treated with Polymer Solution Containing Silver Nanoparticles of Different Sizes and Shapes

Asian Journal of Chemistry ◽

10.14233/ajchem.2020.22266 ◽

2020 ◽

Vol 32 (6) ◽

pp. 1335-1342

Author(s):

Kh. E. Yunusov ◽

A.A. Sarymsakov ◽

S.V. Mullajonova ◽

F.M. Turakulov ◽

S. Sh. Rashidova

Keyword(s):

Silver Nanoparticles ◽

Chemical Properties ◽

Bactericidal Effect ◽

Cotton Fabrics ◽

Sodium Carboxymethylcellulose ◽

Force Microscopy ◽

Transmission Electron ◽

Physico Chemical ◽

Vis Spectroscopy ◽

Silver Nitrate Concentration

Stable silver nanoparticles in solutions of sodium-carboxymethylcellulose (Na-CMC) were synthesized and their structure and physico-chemical properties were evaluated. The form and sizes of silver nanoparticles formed in solutions of CMC and cotton fabrics were studied using UV-VIS spectroscopy, atomic force microscopy and transmission electron microscopy methods. It was found that silver nitrate concentration increase in sodium carboxymethylcellulose solutions, as well as photoirradiation of the hydrogel lead to the changes of the silver nanoparticles size and shape. Investigations have also shown that spherical silver nanoparticles with sizes of 5-35 nm and content of 0.0086 mass% in cotton fabrics possess high bactericidal activity. Stabilization of silver nanoparticles has preserved bactericidal and bacteriostatic activities during the washing of cotton fabrics and textiles on their base.

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Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

Sustainability ◽

10.3390/su12041484 ◽

2020 ◽

Vol 12 (4) ◽

pp. 1484 ◽

Cited By ~ 3

Author(s):

M. Asimuddin ◽

Mohammed Rafi Shaik ◽

Neeshat Fathima ◽

M. Shaistha Afreen ◽

Syed Farooq Adil ◽

...

Keyword(s):

Silver Nanoparticles ◽

Metallic Nanoparticles ◽

Low Cost ◽

Preliminary Investigation ◽

Antibacterial Properties ◽

Silver Ions ◽

Ziziphus Mauritiana ◽

Bacterial Strains ◽

Vis Spectroscopy ◽

Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

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Green Synthesis of Antileishmanial and Antifungal Silver Nanoparticles Using Corn Cob Xylan as a Reducing and Stabilizing Agent

Biomolecules ◽

10.3390/biom10091235 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1235 ◽

Cited By ~ 1

Author(s):

Rony Lucas Silva Viana ◽

Gabriel Pereira Fidelis ◽

Mayara Jane Campos Medeiros ◽

Marcelo Antonio Morgano ◽

Monique Gabriela Chagas Faustino Alves ◽

...

Keyword(s):

Silver Nanoparticles ◽

Synthesis Method ◽

Optical Emission ◽

Molar Ratio ◽

Emission Spectrometry ◽

Corn Cob ◽

Ultrasound Technique ◽

Force Microscopy ◽

Vis Spectroscopy ◽

Agricultural Byproduct

Corn cob is an agricultural byproduct that produces an estimated waste burden in the thousands of tons annually, but it is also a good source of xylan, an important bioactive polysaccharide. Silver nanoparticles containing xylan (nanoxylan) were produced using an environmentally friendly synthesis method. To do this, we extracted xylan from corn cobs using an ultrasound technique, which was confirmed by both chemical and NMR analyses. This xylan contained xylose, glucose, arabinose, galactose, mannose, and glucuronic acid in a molar ratio of 50:21:14:9:2.5:2.5, respectively. Nanoxylan synthesis was analyzed using UV–vis spectroscopy at kmax = 469 nm and Fourier transform infrared spectroscopy (FT-IR), which confirmed the presence of both silver and xylan in the nanoxylan product. Dynamic light scattering (DLS) and atomic force microscopy (AFM) revealed that the nanoxylan particles were ~102.0 nm in size and spherical in shape, respectively. DLS also demonstrated that nanoxylan was stable for 12 months and coupled plasma optical emission spectrometry (ICP-OES) showed that the nanoxylan particles were 19% silver. Nanoxylan reduced Leishmania amazonensis promastigote viability with a half maximal inhibitory concentration (IC50) value of 25 μg/mL, while xylan alone showed no effective. Additionally, nanoxylan exhibited antifungal activity against Candida albicans (MIC = 7.5 μg/mL), C. parapsilosis (MIC = 7.5 μg/mL), and Cryptococcus neoformans (MIC = 7.5 μg/mL). Taken together, these data suggest that it is possible to synthesize silver nanoparticles using xylan and that these nanoxylan exert improved antileishmanial and antifungal activities when compared to the untreated polysaccharide or silver nitrate used for their synthesis. Thus, nanoxylan may represent a promising new class of antiparasitic agents for use in the treatment of these microorganisms.

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Green Synthesis of Biopolymer-Silver Nanocomposites for Gas Sensing

Advances in Science and Technology ◽

10.4028/www.scientific.net/ast.99.54 ◽

2016 ◽

Vol 99 ◽

pp. 54-60 ◽

Cited By ~ 1

Author(s):

Shilpa A. Pande

Keyword(s):

Silver Nanoparticles ◽

Optical Sensors ◽

Gas Sensing ◽

Low Cost ◽

Research Work ◽

Trisodium Citrate ◽

Nanocomposite Films ◽

Sem Images ◽

Natural Polysaccharide ◽

Vis Spectroscopy

In this research work, a very simple, low cost eco-friendly method is presented for the synthesis of silver nanoparticles to be used in colorimetric optical sensors based on localized SPR (LSPR) measurement for gas ammonia. Silver nitrate salts are reduced using gaur gum which acts as a capping and reducing agent. Commonly used reducing agents such as trisodium citrate or sodium borohydride are replaced by a more environmental friendly natural polysaccharide. Nanocomposite films of ~ 1.5 μm thicknesses were fabricated using Gaur Gum and silver nanoparticles. The uniformity of nanoparticles size was measured by SEM and TEM, while face centred cubic structure of crystalline silver nanoparticles was characterized using the X-ray diffraction technique. The optical properties of the composite film were tested by UV-VIS Spectroscopy. The formation of Gaur Gum/silver nanocomposite films was confirmed using SEM images. Also the resistivity of nanocomposite thin film was measured which could be then used for gas sensing application.

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Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Enzyme Research ◽

10.1155/2014/389739 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 19

Author(s):

Kanchana Dumri ◽

Dau Hung Anh

Keyword(s):

Silver Nanoparticles ◽

Soybean Oil ◽

Low Cost ◽

Immobilized Lipase ◽

Complex Surface ◽

Biodiesel Production ◽

Alternative Source ◽

Covalent Bonds ◽

Chromatography Analysis ◽

Vis Spectroscopy

Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10–20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.

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