scholarly journals Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kanchana Dumri ◽  
Dau Hung Anh
Keyword(s):  
Silver Nanoparticles ◽  
Soybean Oil ◽  
Low Cost ◽  
Immobilized Lipase ◽  
Complex Surface ◽  
Biodiesel Production ◽  
Alternative Source ◽  
Covalent Bonds ◽  
Chromatography Analysis ◽  
Vis Spectroscopy

Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10–20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.

scholarly journals A Robust Two-Step Process for the Efficient Conversion of Acidic Soybean Oil for Biodiesel Production

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 527 ◽  
Author(s):  
Gaojian Ma ◽  
Lingmei Dai ◽  
Dehua Liu ◽  
Wei Du
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Lower Cost ◽  
Immobilized Lipase ◽  
Acid Methyl Ester ◽  
Acid Value ◽  
Catalytic Performance ◽  
Raw Material ◽  
Biodiesel Production ◽  
Negative Effect

Acidic oil, which is easily obtained and with lower cost, is a potential raw material for biodiesel production. Apart from containing large quantity of FFAs (free fatty acids), acidic oil usually contains some amount of inorganic acid, glycerides and some other complex components, leading to complicated effect on lipase’s catalytic performance. Exploring the efficient process of converting acidic oil for biodiesel production is of great significance to promote the use of acidic oil. A two-step conversion process for acidic soybean oil was proposed in this paper, where sulfuric acid-mediated hydrolysis was adopted first, then the hydrolyzed free fatty acid, collected from the upper oil layer was further subject to the second-step esterification catalyzed by immobilized lipase Novozym435. Through this novel process, the negative effect caused by harmful impurities and by-product glycerol on lipase was eliminated. A fatty acid methyl ester (FAME) yield of 95% could be obtained with the acid value decreased to 4 mgKOH/g from 188 mgKOH/g. There was no obvious loss in lipase’s activity and a FAME yield of 90% could be maintained with the lipase being repeatedly used for 10 batches. This process was found to have a good applicability to different acidic oils, indicating it has great prospect for converting low quality oil sources for biodiesel preparation.

Green Catalyzing Transesterification of Soybean Oil with Methanol for Biodiesel Based on the Reuse of Waste River-Snail Shell

2010 ◽  
Vol 148-149 ◽  
pp. 794-798 ◽  
Author(s):  
Xiao Hua Liu ◽  
Hai Xin Bai ◽  
Dong Jie Zhu ◽  
Geng Cao
Keyword(s):  
Soybean Oil ◽  
Low Cost ◽  
Production Costs ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Solid Base ◽  
X Ray Diffraction ◽  
Green Catalyst ◽  
Snail Shell ◽  
Diffraction Effects

In this paper, calcined river-snail shell was used as a novel solid base catalyst in the transesterification of soybean oil with methanol for biodiesel production. The calcined river-snail shell was characterized using field emission scanning electron microscope and X-ray diffraction. Effects of transesterification process variables were investigated. The results indicated that river-snail shell calcined at 800 °C catalyzed the transesterification of soybean oil for biodiesel with a yield over 98 % under the conditions including catalyst of 3.0% (w/w), a molar ratio of methanol/oil of 9:1, reaction time of 3 h, and reaction temperature of 65 °C. As a low-cost green catalyst, calcined river-snail shell could not only minimize the environmental wastes resulted from the solid shell, but also reduce the production costs of biodiesel.

scholarly journals Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

2020 ◽  
Vol 12 (4) ◽  
pp. 1484 ◽  
Author(s):  
M. Asimuddin ◽  
Mohammed Rafi Shaik ◽  
Neeshat Fathima ◽  
M. Shaistha Afreen ◽  
Syed Farooq Adil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Low Cost ◽  
Preliminary Investigation ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ziziphus Mauritiana ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

Synthesis and characterization of silver nanoparticles using as a reducing agent plant extract of Dandelion (Taraxacun officianale)

2019 ◽  
pp. 11-17
Author(s):  
Lidia Meléndez-Balbuena ◽  
Eric Reyes-Cervantes ◽  
Blanca Martha Cabrera-Vivas ◽  
Maribel Arroyo
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Plant Extracts ◽  
Low Cost ◽  
Coli Strain ◽  
Reducing Agent ◽  
Force Microscopy ◽  
Yellow Color ◽  
Golden Yellow ◽  
Vis Spectroscopy

The objective of this work was to synthesize the synthesis of silver nanoparticles using as a reducing agent of ionic silver the plant extract of Dandelion (Taraxacun officianale), vegetable of high availability and low cost, as an alternative to the processes conventional, based on the antioxidant capacity of plant extracts that reduce metals in solution. The nanoparticles prepared by this method were characterized by the golden yellow color characteristic of silver nanoparticle solutions. Measurements with UV-Vis spectroscopy of aqueous solutions of Ag1 + ions after coming into contact with plant extracts of Dandelion at different pHs showed an intense absorption band around 400-450 nm, characteristic of the resonance of the Plasmon of silver nanoparticles. Through the scanning of the samples by means of AFM (atomic force microscopy), morphological information of the nanoparticles is obtained, from 3D topographic images of them, such as distribution, size and shape of the silver nanoparticles. Finally, its antibacterial activity was tested against the Escherichia coli strain.

Green Synthesis of Biopolymer-Silver Nanocomposites for Gas Sensing

2016 ◽  
Vol 99 ◽  
pp. 54-60 ◽  
Author(s):  
Shilpa A. Pande
Keyword(s):  
Silver Nanoparticles ◽  
Optical Sensors ◽  
Gas Sensing ◽  
Low Cost ◽  
Research Work ◽  
Trisodium Citrate ◽  
Nanocomposite Films ◽  
Sem Images ◽  
Natural Polysaccharide ◽  
Vis Spectroscopy

In this research work, a very simple, low cost eco-friendly method is presented for the synthesis of silver nanoparticles to be used in colorimetric optical sensors based on localized SPR (LSPR) measurement for gas ammonia. Silver nitrate salts are reduced using gaur gum which acts as a capping and reducing agent. Commonly used reducing agents such as trisodium citrate or sodium borohydride are replaced by a more environmental friendly natural polysaccharide. Nanocomposite films of ~ 1.5 μm thicknesses were fabricated using Gaur Gum and silver nanoparticles. The uniformity of nanoparticles size was measured by SEM and TEM, while face centred cubic structure of crystalline silver nanoparticles was characterized using the X-ray diffraction technique. The optical properties of the composite film were tested by UV-VIS Spectroscopy. The formation of Gaur Gum/silver nanocomposite films was confirmed using SEM images. Also the resistivity of nanocomposite thin film was measured which could be then used for gas sensing application.

scholarly journals ANTIMICROBIAL AND ANTICANCER ACTIVITY OF SILVER NANOPARTICLES FROM EDIBLE MUSHROOM: A REVIEW

2017 ◽  
Vol 10 (3) ◽  
pp. 37 ◽  
Author(s):  
Priyadarshni Karuppiah Chandran ◽  
Mahalingam Pambayan Ulagan
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Low Cost ◽  
Nanoparticle Synthesis ◽  
Colon Adenocarcinoma ◽  
Breast Adenocarcinoma ◽  
Metallic Silver ◽  
Liver Carcinoma ◽  
X Ray ◽  
Vis Spectroscopy

ABSTRACTBiologically inspired nanoparticle synthesis is currently a rapid expanding area of research in nanotechnology. Nanoparticle synthesis utilizing thebioresources such as plants and microbes appears to be a viable, low-cost, and eco-friendly approach. Especially mushrooms can be used for largescalesynthesis of silver nanoparticles as mushroom produces many proteins that reduce the silver nitrate during the biosynthesis. Silver nanoparticlescan be characterized using ultraviolet-visible (UV-VIS) spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction, scanning electronmicroscopy, energy dispersive X-ray, and transmission electron microscope. Silver nanoparticles possess high antibacterial activity since silver indifferent forms has been extensively used as a medicine for curing diseases and promote wound healing. Silver nanoparticles have high surfacespecific area, which will lead to excellent antimicrobial activity as compared with bulk metallic silver. Further, the silver nanoparticles show anticanceractivity against various cell lines such as human epidermoid larynx carcinoma (HEP-2), colon adenocarcinoma (HCT-116), breast adenocarcinoma(MCF-7), liver carcinoma (Hep-G2), and intestinal adenocarcinoma (Caco2) were well documented. This review intends to present green synthesis ofsilver nanoparticles and their application as antimicrobial and anticancer agents.Keywords: Silver nanoparticles, Bioresources, Mushroom, Antimicrobial activity, Anticancer property.

Biodiesel production using immobilized lipase supported on a zirconium-pillared clay. Effect of the immobilization method

2018 ◽  
Vol 16 (11) ◽  
Author(s):  
J.A. Colín-Luna ◽  
E.G. Zamora-Rodea ◽  
M.M. González-Brambila ◽  
E. Barrera-Calva ◽  
R. Rosas-Cedillo ◽  
...  
Keyword(s):  
Edible Oils ◽  
Low Cost ◽  
Immobilized Lipase ◽  
Methyl Esters ◽  
Cost Effective ◽  
Pillared Clay ◽  
Biodiesel Production ◽  
Low Energy Consumption ◽  
Rivers And Lakes ◽  
Heterogeneous Phase

AbstractEdible oils, used in restaurants and households, have become a potential source of environmental pollution because their residuals are indiscriminately poured into rivers and lakes. One cost-effective and sustainable way to treat this waste is using this biomass in the production of biofuels, such as biodiesel. The main reactions for obtaining biodiesel are catalyzed in a homogeneous phase, using basic or acid solutions (NaOH or H2SO4, respectively) or in a heterogeneous phase, using a porous material with or without metals. One interesting reaction, owing to its low energy consumption, is carried out using biocatalysts of enzymes immobilized in porous materials. In this work, a porcine pancreatic lipase (PPL) was immobilized in a zirconium-pillared clay (Zr-PILC) by means of two syntheses: adsorption (PPL/Zr-PILC ADS) and cross-linking (PPL/Zr-PILC CL). The biocatalysts were used in the transesterification of canola oil. The amount of methyl esters was produced in the order Zr-PILC ≈ PPL ≪ PPL/Zr-PILC CL ≪ PPL/Zr-PILC ADS. According to these results, the activity and selectivity are a function of the method of synthesis and show the potential of these biocatalysts to transform waste oil in biodiesel at low cost by means of a sustainable process.

Biogenic of silver nanoparticles using ayurveda tooth powder and its antibacterial activity of extended-spectrum beta-lactamases producing E.coli

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdullah A. Alarfajj ◽  
Mohammedsaleh Almallahi ◽  
Murugan A. Munusamy ◽  
Mickymaray Suresh ◽  
Wael Alturaiki
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Tem Analysis ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Extended Spectrum Beta Lactamases

Extended-spectrum beta-lactamases (ESBL) are enzymes produced by E. coli like some gram negative bacteria. The patients who are affected by ESBL producing bacteria facing a major problem and they may need different β- lactam antibiotics to treat the infection. But this extensive use of β- lactam antibiotics against ESPLs creating major public health threat.  As an alternative currently many eco- friendly, non-toxic, low cost nanoparticles are synthesizing by biogenic way used as an alternative for the β- lactam antibiotics. In the present study silver nanoparticles (AgNPs) were synthesized using Ayurveda Toothpowder. The synthesized AgNPs were characterized using ultraviolet (UV)-visible (vis) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscope (TEM) analysis and finally the antibacterial activity was performed against ESBL producing bacteria by well diffusion method. Antibacterial tests against ESPL producing E.coli cells using biogenic synthesized AgNPs showed significant antibacterial effect at low concentration of AgNPs. The results proved that the biogenic synthesised nanoparticles using Toothpowder extract would help to arrest ESBL producing bacteria a

scholarly journals Controlling of oral pathogens using ginger oleoresin mediated silver nanoparticles

2019 ◽  
Vol 10 (4) ◽  
pp. 2988-2991 ◽  
Author(s):  
Karthikeyan Gayathri ◽  
Anitha Roy ◽  
Thangavelu Lakshmi ◽  
Rajeshkumar S
Keyword(s):  
Silver Nanoparticles ◽  
Low Cost ◽  
Diffusion Method ◽  
Natural Substances ◽  
Antimicrobial Sensitivity ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Test Organisms ◽  
Oral Pathogens ◽  
Agar Well Diffusion Method

Silver nanoparticles have become a substance of great interest among researches in the present time due to its low cost and its wide range of applications in various fields. Moreover, recently, green nanotechnology has been gaining popularity because of its exclusion of damaging reagents and cost-effectiveness. In this study, ginger oleoresin mediated silver nanoparticles were prepared and confirmed with UV-Vis spectroscopy. The ginger oleoresin mediated silver nanoparticles were then tested for its antibacterial activity using agar well diffusion method against Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. The antimicrobial sensitivity was found to be highest against Streotococcus mutans among the test organisms. Silver nanoparticle synthesised using natural substances can be used in control of oral pathogens.

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