scholarly journals Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

2020 ◽  
Vol 12 (4) ◽  
pp. 1484 ◽  
Author(s):  
M. Asimuddin ◽  
Mohammed Rafi Shaik ◽  
Neeshat Fathima ◽  
M. Shaistha Afreen ◽  
Syed Farooq Adil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Low Cost ◽  
Preliminary Investigation ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ziziphus Mauritiana ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

scholarly journals Printable Resin Modified by Grafted Silver Nanoparticles for Preparation of Antifouling Microstructures with Antibacterial Effect

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3838
Author(s):  
Hazem Idriss ◽  
Roman Elashnikov ◽  
Silvie Rimpelová ◽  
Barbora Vokatá ◽  
Petr Haušild ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Polymerization Process ◽  
Resin Matrix ◽  
Bacterial Strains ◽  
Ion Release ◽  
3D Printed

The usage of three-dimensional (3D) printed materials in many bioapplications has been one of the fastest-growing sectors in the nanobiomaterial industry in the last couple of years. In this work, we present a chemical approach for grafting silver nanoparticles (AgNPs) into a resin matrix, which is convenient for 3D printing. In this way, the samples can be prepared and are able to release silver ions (Ag+) with excellent antibacterial effect against bacterial strains of E. coli and S. epidermidis. By the proposed process, the AgNPs are perfectly mixed and involved in the polymerization process and their distribution in the matrix is homogenous. It was also demonstrated that this approach does not affect the printing resolution and the resin is therefore suitable for the construction of microstructures enabling controlled silver ion release and antifouling properties. At the same time the physical properties of the material, such as viscosity and elasticity modulus are preserved. The described approach can be used for the fabrication of facile, low-cost 3D printed resin with antifouling-antibacterial properties with the possibility to control the release of Ag+ through microstructuring.

scholarly journals Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Cannabis Sativa ◽  
Antibacterial Properties ◽  
Green Building ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Vis Spectroscopy ◽  
Ft Ir

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

Immobilization of Silver Nanoparticles on Chitosan-Coated Silica-Gel-Beads and the Antibacterial Activity

2021 ◽  
Vol 892 ◽  
pp. 36-42
Author(s):  
Muhammad Iqbal Hidayat ◽  
Muhammad Adlim ◽  
Ilham Maulana ◽  
Muhammad Zulfajri
Keyword(s):  
Silver Nanoparticles ◽  
Silica Gel ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Chitosan Film ◽  
Silver Ions ◽  
Filter Material ◽  
Silver Ion ◽  
Gel Beads ◽  
Metal Colloid

Silver nanoparticles (Ag0) have attracted the most attention due to their broad antimicrobial application and outstanding activity. The silver nanoparticles are usually in colloidal form, then immobilization the colloid onto solid support is still interesting to explore. In this work, a new method for silver colloidal nanoparticle immobilization on silica gel beads (SiG), which was then symbolized as Ag0-[chi-SiG] was conducted and characterized successfully. The finding proved that SiG must be coated with three chitosan film layers to give stable support for silver nanoparticles. This coating method caused the chitosan completely covered SiG, and the chitosan film provides coordination bonding for silver ions. The most appropriate solvent for silver ion impregnation on the surface of chi-SiG is methanol compared to other solvents. Tungsten lamp as the photo-irradiation, which is low cost and environmentally friendly has been proven effective for silver ion reduction, as shown by silver metal colloid UV-Vis surface plasmon resonance at 400-700 nm. Ag0-[chi-SiG] showed the antibacterial properties of inhibiting the growth Staphylococcus aureus and Escherichia coli; then it provides the potential application for antibacterial filter material. According to the weight comparison between antibacterial standard and Ag content, then Ag0-[chi-SiG] has two and five times higher of exhibiting zone for each bacteria.

scholarly journals Antibacterial Effect of Colloidal Suspensions Varying in Silver Nanoparticles and Ions Concentrations

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Varvara Platania ◽  
Alexandra Kaldeli-Kerou ◽  
Theodora Karamanidou ◽  
Maria Kouki ◽  
Alexander Tsouknidas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Colloidal Suspensions ◽  
Silver Ions ◽  
Cellular Level ◽  
Bacterial Strains ◽  
Three Samples ◽  
Flow Filtration

A lot of effort has been dedicated recently to provide a better insight into the mechanism of the antibacterial activity of silver nanoparticles (AgNPs) colloidal suspensions and their released silver ionic counterparts. However, there is no consistency regarding whether the antibacterial effect displayed at cellular level originates from the AgNPs or their ionic constitutes. To address this issue, three colloidal suspensions exhibiting different ratios of AgNPs/silver ions were synthesized by a wet chemistry method in conjunction with tangential flow filtration, and were characterized and evaluated for their antimicrobial properties against two gram-negative, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and two gram-positive, Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), bacterial strains. The produced samples contained 25% AgNPs and 75% Ag ions (AgNP_25), 50% AgNPs and 50% Ag ions (AgNP_50), and 100% AgNPs (AgNP_100). The sample AgNP_100 demonstrated the lowest minimum inhibitory concentration values ranging from 4.6 to 15.6 ppm for all four bacterial strains, while all three samples indicated minimum bactericidal concentration (MBC) values ranging from 16.6 ppm to 62.5 ppm against all strains. An increase in silver ions content results in higher bactericidal activity. All three samples were found to lead to a significant morphological damage by disruption of the bacterial cell membranes as analyzed by means of scanning electron microscopy (SEM). The growth kinetics demonstrated that all three samples were able to reduce the bacterial population at a concentration of 3.1 ppm. SEM and growth kinetic data underline that S. epidermidis is the most sensitive among all strains against the investigated samples. Our results showed that all three AgNPs colloidal suspensions exhibited strong antibacterial properties and, thus, they can be applied in medical devices and antimicrobial control systems.

scholarly journals Green Synthesis of Ag Nanoparticles for Anti-Microbial Applications using Aloe Barbadensis Miller Extract

2021 ◽  
pp. 233-239
Author(s):  
Sankar S ◽  
Ganga Krishnan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ag Nanoparticles ◽  
Aloe Vera ◽  
Silver Ions ◽  
Xrd Analysis ◽  
Aloe Barbadensis ◽  
Agno3 Solution ◽  
Vis Spectroscopy ◽  
Ft Ir

The green synthesis of nanomaterials is becoming much popular as a result of worldwide problems associated with environmental concerns. In the present work, leaf extract of Barbadensis Miller, (commonly known as Aloe vera) was used for bio-reduction of silver ions to silver nanoparticles. Aloe vera extract and AgNO3 solution in different volumes were treated and it resulted in the reduction of Ag+ ions to Ag metal atoms, which further accumulated as Ag nanoparticles. The prepared nanoparticles were characterized by UV-vis spectroscopy, SEM, FT-IR spectroscopy and XRD analysis. The present study established that the shape and size of the silver nanoparticles can be effectively controlled and modulated using green synthesis technique. The scope of the prepared particles for anti-microbial applications were also investigated.

scholarly journals Rapid green synthesis of silver nano particles from Ziziphus mauritiana and antibacterial activity against human pathogens

2019 ◽  
Vol 41 ◽  
pp. e45262
Author(s):  
Sivaji Asha ◽  
Palaniyandi Thirunavukkarasu
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Silver Ions ◽  
Nano Particles ◽  
Klebsiella Pneumonia ◽  
Ziziphus Mauritiana ◽  
Human Pathogens ◽  
Vis Spectroscopy ◽  
Young Leaves

A novel green source Ziziphus mauritiana fresh young leaves was opted to synthesize silver nanoparticles and analyze its antibacterial activity. The bioactive compounds present in the plant extracts reduced silver ions to NPs, indicated by change in color from red to dark brown. In this study, we have successfully synthesized nanoparticles using Z. mauritiana aqueous leaf extract as a reducing agent and the reaction process of synthesized nanoparticles was monitored by UV-Vis spectroscopy. The UV-Vis absorption peak showed maximum adsorption at 420 nm confirmed the silver nanoparticles synthesis. Further characterization was carried out by FTIR and the results recorded a downward shift of absorption the bands between 400 to 4000 cm-1 indicates the formation of silver nanoparticles. Finally, the present research was exploited to study the antibacterial activity of synthesized nanoparticles produced Z. mauritiana was studied using different pathogenic bacteria such as Salmonella sp., Proteus sp., Bacillus sp., Klebsiella pneumonia and E.coli from the well diffusion results, the synthesized silver nanoparticles displayed the best antibacterial property as compared to the antibiotic has been reported in this paper. To the best of our knowledge, this is the first report that the Z. mauritiana aqueous extract facilitate the synthesis of silver nanoparticles and also exhibits antibacterial activity.

scholarly journals Biosynthesis of Silver Nanoparticles by Marine Invertebrate (Polychaete) and Assessment of Its Efficacy against Human Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Reena Singh ◽  
Sunil Kumar Sahu ◽  
Muthusamy Thangaraj
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Marine Invertebrate ◽  
Average Particle Size ◽  
Silver Ions ◽  
Physical Method ◽  
Average Particle ◽  
Human Pathogens ◽  
X Ray ◽  
Vis Spectroscopy

Synthesis of metallic nanoparticles by chemical and physical method makes the process often cumbersome due the usage of toxic and expensive chemicals. The present study reports the biosynthesis of silver nanoparticles using marine invertebrate (polychaete) extract at room temperature. The ultraviolet-visible (UV-Vis) spectroscopy revealed the formation of silver nanoparticles (AgNPs) by exhibiting the typical surface plasmon absorption maximum at 418–420 nm. Structure and composition of AgNPs were analyzed by atomic force microscopy (AFM). Average particle size of AgNPs ranged from 40 to 90 nm, confirmed by scanning electron microscopy (SEM) analysis. The energy-dispersive X-ray spectroscopy (EDX) of the nanoparticles dispersion confirmed the presence of elemental silver signal, whereas X-ray diffraction (XRD) substantiated the crystalline nature of synthesized nanoparticle. Fourier transform infrared spectroscopy (FTIR) spectral analysis showed the presence of amides phenols, ethers, and fatty acids as major biomolecules responsible for the reduction of silver ions. The possible mechanism responsible for the synthesis of AgNPs by these biomolecules was also illustrated by chemical reactions. The synthesized AgNPs showed comparatively good antibacterial activity against the tested human pathogens. This study advocates that not only plants and microbes but also marine invertebrates do have potential for synthesizing nanoparticles by a cost-effective and eco-friendly approach.

scholarly journals A New Supported Manganese-Based Coordination Complex as a Nano-Catalyst for the Synthesis of Indazolophthalazinetriones and Investigation of Its Antibacterial Activity

Chemistry ◽  
2021 ◽  
Vol 3 (3) ◽  
pp. 783-799
Author(s):  
Maryam Ariannezhad ◽  
Davood Habibi ◽  
Somayyeh Heydari ◽  
Vahideh Khorramabadi
Keyword(s):  
Antibacterial Activity ◽  
Simple Procedure ◽  
Condensation Reaction ◽  
Antibacterial Properties ◽  
Coordination Complex ◽  
Bacterial Strains ◽  
One Pot ◽  
Nano Catalyst ◽  
Ft Ir ◽  
The One

A new magnetic supported manganese-based coordination complex (Fe3O4@SiO2@CPTMS@MBOL@ Mn) was prepared in consecutive stages and characterized via various techniques (VSM, SEM, TEM, XRD, FT-IR, EDX, TG-DTA, and ICP). To evaluate its application, it was used for synthesis of divers Indazolophthalazinetriones in a simple procedure via the one-pot three-component condensation reaction of aldehydes, dimedone, and phthalhydrazide in ethanol under reflux conditions. The Mn catalyst can be recycled without any noticeable loss in catalytic activity. Additionally, the antibacterial properties of the nano-catalyst were studied against some bacterial strains.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

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