scholarly journals A THE EFFECT OF DOSE AND TIME OF ADMINISTRATION OF TRICHODERMA SP ON FUSARIUM OXYSPORUM ON SHALLOT (Allium ascalonicum L)

2021 ◽  
Vol 15 (2) ◽  
pp. 33-39
Author(s):  
Afik Yasintasari Yasintasari ◽  
Pramono Hadi ◽  
Shalahudin Mukti Prabowo

ABSTRACT This studypaimsptopdeterminepthe appropriate dosepand time ofxadministration ofxTrichoderma sp to control Fusarium oxysporum disease in shallot plants. Thepresearch waspconductedpfrompSeptemberp2020ptoxDecemberx2020 in Karangasem Hamlet, Guli Village, Nogosari District, Boyolali Regency. Thispstudypusedpapfactorialpcompletely randomizedpdesign (RAL) methodpwhich consisted ofp2pfactors. Thepfirst treatmentpfactor was thepdose ofpTrichoderma sp (D) which consisted of 3 g, 5g, 7g and the time of administration (T) which was one week before planting, during planting and a week after planting. Parameterspobservedpwerepnumberpof leaves, number ofptubers, weightpof fresh stover, weight of dry stover and intensity of disease attack. The results showed that the treatment with the best Trichoderma sp (D) dose was obtained in the D3 treatment with a dose of 7 grams / liter of solution had a very significant effect on the number of leaves, fresh stover, dry stover, intensity of disease attack and not significantly different on the number of tubers. The best time treatment  for giving Trichoderma sp (T) was obtained in T1 treatment, namely the time of administration one week before planting had a significant effect on the number of leaves, fresh stover, and was not significantly different on the number of tubers, dry stover, intensity of disease attack. Meanwhile, the best combination of dosage and time of administration of Trichoderma sp was obtained in the (D3T1) treatment, namely the administration of a dose of 7 grams / liter of solution and the time of administration one week before planting had a significant effect on dry stover and had no significant effect on other parameters.   Keywords : Trichoderma sp, fusarium oxysporum, shallot.  

2018 ◽  
Vol 15 (1) ◽  
pp. 23-34
Author(s):  
BUKHARI BUKHARI ◽  
NURYULSEN SAFRIDAR

The experimental design used was a 4x4 factorial with 2 replications in a randomized block design (RAK) pattern. The first factor is the Type of Banana (J) consisting of 4 levels: J1 (Raja Banana ), J2 (Ambon Banana), J3 (Barangan Banana) and J4 (Geupok Banana). The second factor was the mixing of Trichoderma Sp with organic manure aplication (D) consisting of 4 levels: D0 (without trichoderma sp), D1 (200 g trichoderma sp in 4 kg manure), D2 (200 gr trichoderma sp in 8 kg manure), and D3 (200 gr trichoderma sp in 12 kg manure). Observations made include: Intensity of Fusarium oxysporum wilting attack, number of leaves, Leaf length and stem diameeter. The results showed that the type of banana had a very significant effect on the intensity of fusarium oxysporum wilt disease and the number of leaves, but no significant effect on the length and diameter of the stem. Barangan Bananas and Ambon Bananas show stronger resilience (more resistant to fusarium oxysporum wilt disease). While the Raja Banana and Geupok Bananas look more sensitive to the attack of the disease. The mixing of Trichoderma Sp with organic manure also significantly influenced the intensity of fusarium oxysporum wilt disease, number of banana leaf and leaf length, but no significant effect on stem diameter. The best dose is found in treatment D1, but not different from D2. An increase in the amount of organic matter manure


Author(s):  
Walter Peraza Padilla ◽  
Martha Orozco Aceves ◽  
Alejandro Esquivel Hernández

Se evaluó el parasitismo in vitro de 14 aislamientos de hongos nematófagos (6 Trichoderma sp., 4 Paecilomyces sp., 2 Fusarium oxysporum, y 2 Monacrosporium sp.) contra juveniles y huevos del nematodo agallador Meloidogyne javanica. Los hongos, de 3 regiones productoras de arroz en Costa Rica, (Pacífico Central, Huetar Atlántica y Chorotega), se aislaron mediante el método de espolvoreado en placas, durante abril del 2008 a agosto de 2009. Se utilizó un diseño completamente al azar con 10 repeticiones por hongo, en agar-agua (AA). Se inoculó una suspensión de 1 a 1,5x10 6 conidios (ufc.ml -1 ) por plato Petri, y al cuarto día se depositó en 0,5 ml una suspensión de 150 juveniles y 100 huevos de M. javanica previamente desinfectados, a temperatura ambiente (23-26°C) y con luz natural. Transcurridas 96 h se contó en cada plato el número de nematodos vivos, muertos ó parasi- tados y de huevos parasitados o no parasitados. Se realizó un análisis de regresión logística (RL) para las variables evaluadas, el cual mostró diferencias significativas (p<0,0001) en el desempeño de los hongos evaluados, presentando rangos de entre 10% a 56% en nematodos muertos, de 13% a 79% en nematodos parasitados y de 1% a 96% en huevos parasitados. En general, los hongos del género Trichoderma (Tri1, Tri2, Tri3 y Tri4) fueron los más eficientes en el parasitismo sobre juveniles y huevos de M. javanica.


2019 ◽  
Vol 10 (8) ◽  
pp. 1783-1796
Author(s):  
Carmela Hernandez Domínguez ◽  
José Andrés Vázquez Benito ◽  
Fabian Vázquez Moreno ◽  
Raul Berdeja Arbeu ◽  
Sigfrido David Moráles Fernández ◽  
...  

Se estudió la ocurrencia y diversidad de especies de Fusarium y Trichoderma en 29 puntos de una parcela de plátano (Musa AAB) en Veracruz, México, durante noviembre de 2017 y abril 2018. La parcela se dividió en dos partes; en la primera se realizó barbecho y eliminación de maleza (procedimiento en cultivo 1) mientras que en la segunda no se realizaron estas actividades (procedimiento en cultivo 2). Se aislaron hongos del suelo colectado en estos sitios usando papa dextrosa y agar (PDA) y K2. Al momento de colectar las muestras se registró la temperatura, precipitación y radiación solar. Los resultados del análisis mostraron 68% más presencia de unidades formadoras de colonias (UFC) en suelos donde no se realizaron labores de cultivo usando K2 a diferencia de suelos donde sí se realizaron éstas usando PDA (32%). También se observó poca cantidad de UFC cuando los valores de temperatura, precipitación y radiación fueron altos. Los análisis moleculares mostraron 65% más abundancia de especies en suelos sin labor comparado con suelos cultivados (35%), seis de 16 aislados de Fusarium fueron Fusarium oxysporum f. sp. melonis y seis de 13 aislados de Trichoderma fueron Trichoderma longibrachiatum en el primer muestreo mientras que nueve de 14 aislados de Trichoderma fueron Trichoderma spirale en el segundo muestreo. El análisis de la diversidad intrapoblacional de siete aislados de Trichoderma spirale, mostraron bandas de 75 a 500 bp en tres locis del genoma en el aislado VB28IT1 con cinco ISSR.


2020 ◽  
Vol 10 (2) ◽  
pp. 147-154
Author(s):  
NUR AINU FARHAH RABAE ◽  
SALMAN AZIZ ◽  
ASAMOAH FREDERICK OSEI ◽  
SITI NORDAHLIAWATE MOHAMED SIDIQUE

Piper nigrum L. is well-known as the king of spices and widely used in various field such as food and medicines. In Malaysia, 98% of pepper production comes from the state of Sarawak. The National Commodity Policy (2011-2020) targets to increase the pepper plantation area from the current 16,331 ha to 20,110 ha by year 2020. However, pepper diseases remain as a major challenge in the pepper industry. A great number of airborne fungi pathogen may contribute to a significant economic loss in pepper production. Therefore, this study aims to morphologically identify the diversity of fungi obtained from air-borne samples in a pepper planation that are capable of causing pepper plant diseases. This experiment was conducted at a pepper plantation near Lembah Bidong, Kuala Terengganu. An Andersen spore sampler was used to collect the fungi spores. Culture based identification were then made. The study resulted in the identification of four genus of fungi such as Fusarium sp, Fusarium semictectum Fusarium oxysporum, Curvularia sp., Penicillium sp. and Trichoderma sp. (Ascomycetes). Further molecular identification will confirm the species of fungal pathogens and more understanding of their population as well as severity.   Keywords: Pepper, Piper nigrum L., air-borne, fungi, Andersen spore sampler


Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Nur Azizatu Rohmah

This study aims to determine the effect of giving various kinds and duration of ZPT immersion on the growth of tin plant cuttings (Ficus carica L.). This research was carried out in October - December 2020, at the Terdana Village, Pondok Kubang District, Central Bengkulu Regency. This study used a Factorial Completely Randomized Design (CRD) with two factors, the first factor was giving various PGR (Z) which consisted of Control, Rootone-F, Shallot Solution, Bamboo Shoots Solution, while the second factor was the duration of immersion of PGR which consisted of of 12 hours, 18 hours, 24 hours, each treatment was repeated 3 times. The results of the study were analyzed using Variety Analysis and if it had a significant effect, further tests were carried out using Duncan's Multiple Range Test (DMRT) level 5%. The results showed that there was an interaction between the administration of Rootone-F and the immersion time of ZPT 12 to 24 hours on the percentage of live cuttings of Tin (Ficus carica L.) plant. 12 WAP, root length, percentage of live cuttings and leaf area on cuttings growth of Tin (Ficus carica L.) PGR immersion time treatment was not able to increase shoot length 12 WAP, number of shoots 12 WAP, number of leaves 12 WAP, root length, percentage of cuttings Life and leaf area on the growth of cuttings of Tin (Ficus carica L.) It is recommended to increase the concentration of natural PGR so that it can be used as a substitute for Rootone-F.


2020 ◽  
Vol 2 (1) ◽  
pp. 44-54
Author(s):  
Roy Fernando ◽  
Asmah Indrawati ◽  
Azwana Azwana

Onion is one of predominant flavor in Indonesia culinary which its quantity should be increased contantly. The aim of research was to invesitigate the respond of onion growth toward the treatment combination between POC cabbage and Tree types of fruit skin. This research was performed in a Factorial Randomized Block Design (RBD), which consists of 2 treatment factors, namely: 1) compost 2) liquid organic fertilizer of cabbage waste. The parameters observed in this study were plant height, number of leaves, number of tubers per sample plant, production per sample, production per plot and percentage of plant disease attack. As for the results obtained from this study, namely: 1) Giving various types of fruit skin compost did not significantly affect height, number of leaves, number of tubers, production per sample plant and production per plot of onion plants; 2) liquid organic fertilizer cabbage application has no significant effect on height, number of leaves, number of tubers and production per sample plant, but has a significant effect on production per plot of onion plant; and 3) Combination between the fruits compost and the liquid organic fertilizer that is not real influence on the plant height, the number of leaves, the number of bulbs and production per crop samples, but the effect is very real towards production per plot of the anion plants


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