scholarly journals POLA KUMAN DAN UJI KEPEKAANNYA TERHADAP ANTIBIOTIKA PADA PENDERITA OTITIS EKSTERNA DI POLIKLINIK THT-KL BLU RSU PROF. DR. R. D. KANDOU MANADO

e-CliniC ◽  
2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Pingkan Suwu ◽  
Constantyn Kountul ◽  
Olivia Waworuntu

Abstract: Otitis externa is an outer ear canal infection divided into acute and chronic forms based on the course of illness. In a study at Polyclinic ENT-TOS at Prof. dr. R. D. Kandou General Hospital Manado in 2011 it was found that from 5297 there were 440 (8.33%) cases of otitis externa. This study aimed to determine the pattern of bacteria and antibiotic sensitivity in patients with otitis externa in the Polyclinic ENT-TOS, Prof. Dr. R.D. Kandou Hospital Manado during the period December 2012 - January 2013. This study was a descriptive prospective through a sampling technique of patients. Of the 20 samples tested, 18 (90%) showed the growth of bacteria, and two samples (10%) had no growth of bacteria. There were seven types of bacteria found consisting of  two samples (10%) of Branhamella catarrhalis, four samples (20%) of Staphylococcus epidermidis, two samples (10%) of Staphylococcus aureus, two samples (10%) of Acinetobacter anitratus, two samples (10%) of Enterobacter aerogenes, four samples (20%) of Alcaligenes faecalis, two samples (10%) of Proteus mirabilis, and two samples (10%) of no bacterial growth. The highest sensitivity antibiotic was levofloxacin in 14 samples (77.78%), while the highest resistant to antibiotics was ever getting clindamycin in 16 samples (88.89%). Conclusion: nearly all of the samples obtained from otitis externa patients in Polyclinic ENT-TOS, Prof. Dr. R.D. Kandou Hospital Manado during the period December 2012 - January 2013 showed bacterial growth. Levofloxacin was the highest sensitive antibiotic while clindamycin and erythromycin were the highest resistant antibiotic among otitis externa patients in ENT-TOS Clinic of Prof. Dr. R.D. Kandou Hospital, Manado Keywords: otitis externa, pattern of bacteria, sensitivity test.     Abstrak: Otitis eksterna adalah infeksi liang telinga luar yang menurut perjalanan penyakit terbagi atas bentuk akut dan kronis. Pada penelitian di Poliklinik THT-KL RSU Prof. Dr. R.D. Kandou Manado pada tahun 2011 diperoleh dari 5.297 pengunjung terdapat 440 (8,33%) kasus otitis eksterna. Penelitian ini bertujuan untuk mengetahui pola kuman dan uji kepekaannya terhadap antibiotika pada penderita otitis eksterna di Poliklinik THT-KL BLU RSU Prof. Dr. R.D. Kandou Manado periode Desember 2012 - Januari 2013. Penelitian ini bersifat prospektif deskriptif dan sampel diambil dari pasien Poliklinik THT-KL. Dari 20 sampel yang diuji, didapatkan 18 sampel (90%) menunjukkan pertumbuhan bakteri dan dua sampel (10%) tidak memperlihatkan pertumbuhan bakteri. Bakteri yang ditemukan tujuh jenis, terdiri dari Branhamella catarrhalis dua sampel (10%), Staphylococcus epidermidis empat sampel (20%), Staphylococcus aureus dua sampel (10%), Acinetobacter anitratus dua sampel (10%), Enterobacter aerogenes dua sampel (10%), Alcaligenes faecalis empat sampel (20%), Proteus mirabilis dua sampel (10%), dan tidak ada pertumbuhan bakteri sebanyak dua sampel (10%). Simpulan: hampir semua sampel yang diperoleh dari pasien otitis eksterna di Poliklinik THT-KL RSU Prof. Dr. R.D. Kandou Manado periode Desember 2012 - Januari 2013 memperlihatkan pertumbuhan bakteri. Antibiotika yang kepekaannya paling tinggi ialah levofloxacin, sedangkan antibiotika yang resistensinya paling tinggi ialah clindamycin an erythromycin. Kata kunci: otitis eksterna, pola kuman, uji kepekaan.

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Rinny Olivia Sembiring

Abstract:Tonsillitis is inflammation of palatine tonsil as part of the Waldeyer’sring. Tonsillitis most commonly caused by viral orbacterial infection. The purpose is to determine such an insidence of the type of bacterials from patient’s (with tonsillitis) throat swabs examination at the Department of Otolaryngology RSUP Prof. Dr. R.D Kandou Manado and also to determine such an insidence of the bacterial’s sensitivity ofantibiotics that commonly used on tonsillitis case. This research was taken at the Department of Otolaryngology RSUP Prof. Dr.R.D Kandou Manado by using prospective descriptive study method as well as examining 20 samples from the patients with tonsilitis. The result, 75% samples showed bacterial’s growth. There were found 6 types of bacterials. The following bacterials are 10% eschericia coli, 10% of staphylococcus aureus, 40% of streptococcus sp., 5% of branhamella catarrhalis, 5% of enterobacter aerogenes and 5% of alcaligenes faecalis. On sensitivity of antibiotics, 66,67% Levofloxacin, 66,67% of Cefriaxon, 53,3% of Amoxicilin clavulanic, 13,3% Ciprofloxacin. The differences in place, time, and history of antibiotics therapy, could caused the differences in type of bacterials among the tonsillitis patients.Keywords: tonsillitis, type of bacterial, sensitivity testAbstrak:Tonsilitis merupakan peradangan tonsil palatina yang merupakan bagian dari cincin waldeyer.Tonsilitis adalah infeksi (virus atau bakteri) dan inflamasi pada tonsil.Tujuan penelitian ini mendapatkan gambaran jenis kuman pada pemeriksaan hapusan tenggorokan penderita tonsilitis di Poliklinik THT-KL BLU RSUP Prof. Dr. R. D. Kandou Manado dan juga gambaran tentang kepekaan kuman terhadap beberapa antibiotika yang biasa digunakan pada tonsilitis. Penelitian ini bersifat prospektif deskriptif lewat pengambilan sampel dari pasien Poliklinik THT-KL.Dari 20 sampel yang diuji, didapatkan 15 sampel (75%) menunjukkan pertumbuhan bakteri dan 5 sampel (25%) tidak ada pertumbuhan bakteri. Bakteri yang ditemukan ada6 jenis bakteri yang terdiri dari 2 sampel escherichia coli (10%), 2 sampel staphylococcus aureus (10%), 8 sampel streptococcus sp (40%), 1 sampel branhamella catarrhalis (5%), 1 sampel enterobacter aerogenes (5%), 1 sampel alcaligenes faecalis(5%).Sedangkan sensitifitas antibiotika yang paling tinggi adalah Levofloxacin 10 sampel (66,67%) dan cefriaxone 10 sampel (66,67%), kemudian amoxicilin calvulanic 8 sampel (53,33%), ciprofoxacin 2 sampel (13,33%).Perbedaan tempat, waktu, serta riwayat pernah mendapatkan terapi antibiotika, dapat menyebabkan perbedaan pola kumanKata kunci: tonsilitis, pola bakteri, uji kepekaan


2021 ◽  
Vol 8 (02) ◽  
pp. 80-84
Author(s):  
Smitha B ◽  
Swapna U.P ◽  
Salilkumar K

BACKGROUND Active mucosal chronic otitis media (COM) is a disease of the middle ear cleft associated with inflammation and production of pus. The incidence is high in developing countries. It affects all age groups and both genders. If not properly treated, it can lead to complications. The bacterial isolates and their sensitivity pattern vary from place to place and also over time. Early identification of the microorganisms and their antibiotic sensitivity patterns helps in proper selection of antibiotics and quick recovery in COM patients. METHODS Fifty patients with active mucosal COM were selected for the study. The study was conducted over a period of one year. Ear swab from the deep external auditory canal was collected under strict aseptic precautions from these patients before starting antibiotic treatment. Microorganisms were identified by gram staining, growth on different agar plates, and various biochemical tests. Antibiotic sensitivity was done by the Kirby-Bauer method. RESULTS Majority of the patients were in the age group of 21 - 40 years (42 %) with female preponderance (62 %). All patients had a central perforation in the tympanic membrane (100 %). Bacterial growth was seen in 44 samples (88 %). Two samples (4 %) showed fungal growth. Four samples (8 %) showed no growth even after 48 hours of incubation. Single bacterium was isolated in 41 cases (82 %) whereas 3 samples (6 %) showed more than one bacterial growth. The predominant bacteria were Pseudomonas aeruginosa (50 %) followed by Staphylococcus aureus. The other isolates were coagulase negative staphylococci, enterococci, enterobacter, acinetobacter, and E coli. Pseudomonas showed maximum sensitivity to amikacin, imipenem and piperacillin / tazobactam (100 %). The most effective antibiotics for staphylococcus was vancomycin and linezolid. CONCLUSIONS The most common bacteria isolated in active mucosal COM were pseudomonas followed by Staphylococcus aureus. The most effective antibiotics for pseudomonas was amikacin, imipenem and piperacillin / tazobactam. Staphylococcus showed maximum sensitivity to vancomycin and linezolid. KEYWORDS Chronic Otitis Media (COM), Active Mucosal, Ear Swab, Bacteriology, Sensitivity


Author(s):  
T. O. Olowomofe ◽  
J. O. Oluyege ◽  
A. O. Ogunlade ◽  
D. T. Makinde

Bed linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The present study was aimed at isolating and characterizing bacteria and fungi from different kinds of bed linen of student in Ekiti State University hostels. Pour plate method was used for the enumeration of total bacteria count from the posterior and anterior end of the bed linen. The average bacteria count for the anterior and posterior end was 7.46 and 7.16 Log10 CFU/ml respectively. The most dominant microbial species were bacteria and these were mostly found in the environment and on human skin. The bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter aerogenes, Bacillus cereus, Escherichia coli and Klebsiella aerogenes. Bacillus cereus had the highest frequency of occurrence (25%), followed by Staphylococcus aureus (15%), Escherichia coli (15%), Klebsiella aerogenes (15%), Staphylococcus epidermis (15%), Enterobacter aerogenes (10%) and Proteus mirabilis (5%). The fungi isolates were Aspergillus sulphureus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Fusarium spp. Antibiotics susceptibility test was carried out on the bacteria isolates with gram negative bacteria showing resistance to Cotrimoxazole and gram positive bacteria showing resistance to Amplicillin. Most of the bacteria isolates have multiple antibiotics resistance. The bacterial isolates were susceptible to Ciprofloxacin. Plasmid profiling was also done with Escherichia coli having three high molecular weight plasmids, Bacillus cereus and Enterobacter aerogenes do not possess plasmid. The identified species are suspected to be opportunistic pathogens for human, representing a risk for people with weakened immune system, especially in cases of super-infection.


2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Ashima Sonita ◽  
Erly Erly ◽  
Machdawaty Masri

AbstrakPeningkatan resistensi bakteri penyebab PPOK terhadap beberapa antibiotika yang lazim digunakan oleh klinisi dalam pemberian terapi empirik akan menyebabkan berkurangnya keefektifan terhadap terapi PPOK. Hal ini akan berdampak semakin tingginya morbiditas dan mortalitas yang disebabkan oleh PPOK. Penelitian ini bertujuan untuk mengetahui pola resistensi bakteri penyebab PPOK terhadap beberapa antibiotika di Laboratorium Mikrobiologi RSUP.Dr.M.Djamil periode 2010 – 2012. Penelitian ini telah dilaksanakan pada bulan November 2013 sampai dengan Desember 2013 di Laboratotium Mikrobiologi RSUP Dr.M.Djamil Padang. Metode penelitian yang digunakan adalah deskriptif retrospektif. Hasil penelitian mendapatkan bakteri penyebab PPOK adalah Klebsiella spp (42,44%), Streptococcus α hemolyticus (38,37%), Pseudomonas aeruginosa (12,21%), Staphylococcus aureus (4,65%), Proteus mirabilis (1,16%), Staphylococcus epidermidis (0,58%) dan Streptococcus pneumoniae (0,58%). Disimpulkan bahwa bakteri tersering pada sputum pasien PPOK adalah Klebsiella spp. Tingkat resistensi tertinggi adalah Ampicillin, sedangkan tingkat kepekaan tertinggi adalah terhadap Netilmycin.Kata kunci: antibiotika, bakteri, resistensiAbstractIncreased resistance of bacteria that cause COPD to some antibiotics that are commonly used by clicicians in the provision of empiric therapy, it will cause a reduction in the effectiveness of the tratment of COPD. This study aimed to determine the resistance pattern of Bacteria in sputum of patients COPD to some antibiotics in Microbiology Laboratory Dr.M.Djamil Hospital Peroid 2010 – 2012. The research method was a descriptive and retrospective. According to the result, the bacteria that causes COPD is Klebsiella spp (42.44%), Streptococcus α hemolyticus (38.37%), Pseudomonas aeruginosa (12.21%), Staphylococcus aureus (4.65%), Proteus mirabilis (1.16%), Staphylococcus epidermidis (0.58%), and Streptococcus pneumoniae (0.58%). It can be concluded that the bacteria which cause the highest number of COPD is Klebsiella sp. The highest level of resistance is ampicillin while the highest level of sensitivity Is netilmycin.Keywords: antibiotics, bacteria, resistance


2020 ◽  
Vol 3 (2) ◽  
pp. 96
Author(s):  
Isna Romadhona ◽  
Fauna Herawati ◽  
Rika Yulia

Antibiotik merupakan obat yang digunakan untuk mengatasi dan mencegah infeksi bakteri. Penggunaan antibiotik yang tidak tepat dapat menimbulkan berbagai masalah, diantaranya pengobatan akan lebih mahal dan juga risiko terjadinya resistensi bakteri terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui profil penggunaan antibiotik dan profil peta kuman pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik serta untuk mengetahui kesesuaian penggunaan antibiotik dengan mengacu pada Permenkes Republik Indonesia No. 2406/Menkes/PER/XII/2011. Data penggunaan antibiotik diperoleh dari catatan Rekam Medis pada periode Januari – November 2017. Data penggunaan antibiotik dihitung dengan menggunakan rumus DDD/100 pasien-hari rawat. Hasil perhitungan DDD/100 pasien-hari rawat menunjukkan hasil sebesar 470,11 DDD/100 pasien-hari rawat. Peta kuman pada pasien gangren, melaporkan adanya bakteri Enterobacter cloacae 24%, Escherichia coli 18%, Staphylococcus aureus 15%, Acinetobacter baumannii 9%, Pseudomonas aeruginosa 6%, Citrobacter youngae 6%, Enterobacter aerogenes 6%, Proteus vulgaris 6%, Staphylococcus schleiferi 6%, Klebsiella pneumoniae 3%, dan Proteus mirabilis 3% . Penggunaan antibiotik seftriakson dan metronidazol pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik pada periode Januari – November 2017 telah sesuai dengan pedoman penggunaan antibiotik berdasarkan Permenkes Republik Indonesia No. 2406/Menkes/PER/ XII/2011, yaitu antibiotik golongan sefalosporin generasi III yang lebih aktif terhadap Enterobacteriaceae dan antibiotik golongan nitroimidazol yang dapat mengobati infeksi bakteri basil anerob Gram-Negatif.


2020 ◽  
Vol 6 (1) ◽  
pp. 101
Author(s):  
Citra Sepriana ◽  
Eti Sumiati

This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.


2014 ◽  
Vol 8 (4) ◽  
pp. 27-33
Author(s):  
E. J. AL-Kalifawi

The study was conducted from January to March 2012. In this study colorimetric VITEK-2 Compact system used for its accuracy and rapidity to identify isolates and to detect several antimicrobial resistances.The study also investigate the antibacterial effect of Kombucha tea on isolated bacteria from diabetic foot ulcer. The bacteria isolated were eight gram negative bacteria, namely, Acinetobacter baumannii 3 (2%), Enterobacter cloacae 5 (4%), Escherichia coli 13 (10%), Klebsiella pneumoniae 7 (6%), Citrobacter spp. 4 (3%), Proteus mirabilis 3 (2%), Proteus vulgaris 3 (2%) and Pseudomonas aeruginosa 44 (35%). Four gram positive bacterium, Enterococcus faecalis 6 (5%), Staphylococcus aureus 17 (13%), Staphylococcus epidermidis 13 (10%) and Streptococcus spp. 9 (8%). The antimicrobial activities of antibiotics showed that, all isolates are sensitive to Ciprofloxacin, Levofloxacin and Ofloxacin. The resistance to other types of antimicrobial differ with different isolate. The effect of Kombucha tea on all isolates wasclear at 7days of incubation; the diameter of inhibition was 6mm for Acinetobacter baumannii, Proteus vulgaris and Enterococcus faecalis. 7mm for Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Citrobacter spp., Staphylococcus epidermidis and Streptococcus spp. 8mm for Proteus mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus. The maximum activity of fermented tea was recorded at 14days incubation of Kombucha organism against all isolates, the diameter of inhibition was 21mm for Acinetobacter baumannii, 24mm for Enterobacter cloacae, 23mm for Escherichia coli, Staphylococcus epidermidis and Streptococcus spp., 16mm for Klebsiella pneumoniae, 22mm for Citrobacter spp. and Enterococcus faecalis, 25mm for Proteus mirabilis and Staphylococcus aureus, 20mm for Proteus vulgaris, 26mm for Pseudomonas aeruginosa. The antibacterial activity of Kombucha tea decrease with increase incubation periods28 days.


2019 ◽  
Vol 10 (2) ◽  
pp. 152
Author(s):  
Herman Herman ◽  
Rahman Rahman ◽  
Hari Asti

Deteksi dini sepsis sangat penting karena sepsis memiliki angka mortalitas dan morbiditas sangat tinggi. Kultur darah dan prokalsitonin adalah pemeriksaan untuk mendeteksi sepsis di RSUP DR Wahidin Sudirohusodo tetapi biasanya hasil prokalsitonin tidak sesuai dengan hasil kultur darah. Tujuan penelitian ini untuk menganalisis prokalsitonin dan kultur darah sebagai penanda sepsis di RSUP DR Wahidin Sudirohusodo Makassar. Ini adalah penelitian observasi laboratorik yang bersifat deskriptif dengan teknik consequtive sampling. Berdasarakan penelitian ini didapatkan hasil kultur darah berupa bakteri aerob, seperti Staphylococcus hominis ssp hominis, Staphylococcus haemolyticus, Staphylococcus aureus, Klebsiella pneumoniae ssp pneumoniae, Enterococcus faecalis, Escherichia coli, Burkholderia cepacia, Staphylococcus epidermidis, Enterobacter aerogenes, Burkholderia pseudomallei, Acinetobacter baumannii, Aermonas hydrophila, dan Enterobacter cloacae complex serta disimpulkan pula Prokalsitonin memiliki sensitivitas 100 % ,spesifisitas 65 %, akurasi 24 %. Pada penelitian yang dilakukan menunjukkan bahwa Prokalsitonin memiliki sensitivitas yang sangat tinggi namun proklasitonin memiliki spesifitas dan akurasi yang rendah sebagai penanda sepsis di RSUP DR Wahidin Sudirohusodo Makassar.Kata kunci : Kultur Darah, Prokalsitonin, RSUP DR Wahidin Sudirohusodo Makassar, Sepsis


1997 ◽  
Vol 106 (9) ◽  
pp. 751-752 ◽  
Author(s):  
Itzhak Brook

External otitis caused by Staphylococcus aureus was observed in a nurse after extensive use of a stethoscope. The infection recurred and a similar organism was isolated from the stethoscope's earpiece. The infection did not recur after the earpiece was cleansed after each use. In a prospective study, the bacterial flora of 35 earpieces was evaluated. Fifty-three isolates, 36 aerobic or facultative and 17 anaerobic, were recovered. The number of organisms per earpiece ranged from 14 to 204 (average 92 ± 17). The predominant isolates were Staphylococcus epidermidis (16 isolates), Propionibacterium acnes (12), and S aureus (7). The study demonstrates the colonization of the stethoscope's earpiece with microorganisms that possess the potential for causing nosocomial infection.


2019 ◽  
Vol 8 (2) ◽  
pp. 67-74
Author(s):  
Ringga Novelni

Ulkus diabetikum adalah salah satu komplikasi kronis diabetes melitus berupa luka terbuka pada permukaan kulit yang dapat disertai adanya kematian jaringan. Kadar glukosa darah yang tinggi menjadi tempat strategis perkembangan bakteri. Penelitian ini bertujuan untuk mengetahui bakteri penyebab ulkus diabetikum dan resistensinya terhadap antibiotik. Jenis penelitian ini adalah Deskriptif Eksploratif. Pada penelitian ini didapatkan 10 sampel pasien ulkus diabetikum, 4 sampel Klebsiella pneumonia, 2 sampel Staphylococcus epidermidis dan 1 sampel Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris dan Proteus mirabilis. Kemudian dilakukan identifikasi bakteri dan uji resisitensi antibiotik terhadap 5 jenis antibiotik dengan menggunakan metode difusi. Jenis bakteri yang banyak ditemukan dalam pus ulkus diabetikum berturur-turut adalah Klebsiella pneumonia (40%), Staphylococcus epidermidis (60%). Proteus vulgaris (10%), Proteus mirabilis (10%), Pseudomonas aeruginosa (10%) dan Staphylococcus aureus (10%). Ciprofloksasin dan cefotaxime menduduki peringkat tertinggi tingkat resistensi dengan jumlah resistensi bakteri terbanyak (40%), disusul Meropenem dan Gentamisin (30%), sedangkan Vankomisin efektif digunakan pada bakteri Staphylococcus aureus dan Staphylococcus epidermidis dengan tingkat resistensi (0%).


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