Uji konsentrasi hambat minimum (KHM) ekstrak daun binahong (Anredera cordifolia Steenis) sebagai antibakteri terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Klaudya E. Warokka ◽  
Jane Wuisan ◽  
Juliatri .
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Test Methods ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Study Program ◽  
Group Design

Abstract: Inadequate oral and dental hygiene can cause plaques containing various kinds of bacteria; one of them is Streptococcus mutans which is the main cause of dental caries. Binahong leaf (Anredera cordifolia Steenis) is a medicinal herb that contains antibacterial compounds namely flavonoids, alkonoid, terpanoid, and saponins. This study aimed to determine the minimal inhibitory concentration (MIC) of binahong leaf extract to the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. The method used in this study was serial dilution method with turbidimetry and spectrophotometry as the test methods. Binahong leaves were taken from Tempok village and were extracted with maceration method using ethanol 96%. Streptococcus mutans bacteria were obtained from a pure bacterial stock in Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Science University of Sam Ratulangi Manado. The result showed that the minimal inhibitory concentration (MIC) of binahong leaf extract (Anredera cordifolia steenis) to the growth of Streptococcus mutans was 6.25%.Keywords: Streptococcus mutans, binahong leaf, MIC, tooth cariesAbstrak: Kebersihan gigi dan mulut yang buruk dapat menyebabkan terbentuknya plak yang mengandung berbagai macam bakteri, salah satu diantaranya Streptococcus mutans yang menjadi penyebab utama terjadinya karies gigi. Daun binahong (Anredera cordifolia steenis) merupakan tanaman herbal yang mengandung senyawa antibakteri yaitu flavonoid, alkonoid, terpanoid dan saponin. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari daun binahong terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Penelitian ini menggunakan metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun binahong diperoleh di desa Tempok, dan diekstraksi dengan metode maserasi menggunakan etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan bahwa KHM daun binahong terhadap pertumbuhan streptococcus mutans ialah pada konsentrasi 6,25%.Kata kunci: Streptococcus mutans, daun binahong, KHM, karies gigi

scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L. Medik) terhadap Pertumbuhan Streptococcus mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Benedicta N. D. Rori ◽  
Johanna A. Khoman ◽  
Aurelia S. R. Supit
Keyword(s):  
Dental Caries ◽  
Minimal Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Group Design ◽  
Extract Concentration

Abstract: The main problem in dental health is dental caries that occurs due to the fermentation by Streptococcus mutans bacteria. Gedi leaf (Abelmoschus manihot L. Medical) is one of the common plants in Northern Celebes that contains antimicrobial compounds namely flavonoid, alkaloid, steroid, and saponin; all of them have been proved to inhibit the growth of S. mutans. This study was aimed to determine the minimal inhibitory concentration (MIC) of gedi leaf extract (Abelmoschus manihot L. Medical) to S. mutans growth. This was a true experimental study with a randomized pretest-posttest control group design. The method used in this study was serial dilution method with turbidimetry and spectrophotometry. Samples of gedi leaves were obtained at Paal 2 Manado, and were extracted with maceration method using ethanol 96%. S. mutans bacteria were obtained from the pure bacterial stock at Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Science University of Sam Ratulangi Manado. The turbidimetry test showed that the tube content became clearer at 25% of extract concentration. Moreover, the UV-Vis spectro-photometer showed a decrease of OD value for the first time at 25% of extract concentration. Conclusion: The minimal inhibitory concentration (MIC) of gedi leaf extract (Abelmoschus manihot L. Medical) to Streptococcus mutans growth was at concentration of 25%.Keywords: gedi leaf (Abelmoschus manihot L. Medical), Streptococcus mutans, MIC, dental caries Abstrak: Masalah utama dalam kesehatan gigi ialah karies gigi yang terbentuk karena proses peragian oleh bakteri Streptococcus mutans. Daun gedi (Abelmoschus manihot L. Medik) merupakan salah satu tanaman khas daerah Sulawesi Utara yang mengandung senyawa antimikroba berupa flavonoid, alkaloid, steroid, dan saponin yang telah terbukti memiliki efek antibakteri terhadap pertumbuhan S. mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) ekstrak daun gedi (Abelmoschus manihot L. Medik) terhadap pertumbuhan S. mutans. Jenis penelitian ialah eksperimental murni dengan pretest-posttest control group design. Metode yang digunakan dalam penelitian ini yaitu metode serial dilusi dengan pengujian turbidimetri dan spektrofotometri. Daun gedi diperoleh dari Kecamatan Paal 2 Manado, dan diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri S. mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil pengujian turbidimetri memperlihatkan bahwa larutan dalam tabung terlihat mulai jernih pada konsentrasi ekstrak 25%. Pada pengujian spektrofotometer UV-Vis terlihat penurunan nilai OD pertama kali pada konsentrasi 25%. Simpulan: Konsentrasi hambat minimum ekstrak daun gedi (Abelmoschus manihot L. Medik) terhadap pertumbuhan Streptococcus mutans terdapat pada konsentrasi 25%.Kata kunci: daun gedi (Abelmoschus manihot L. Medik), Streptococcus mutans, KHM, karies

scholarly journals Uji Konsentrasi Hambat Minimum (KHM) Ekstrak Daun Mengkudu (Morinda citrifolia L.) terhadap Candida albicans Secara In Vitro

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Lisa Ramschie ◽  
Pieter L. Suling ◽  
Krista V. Siagian
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Group Design

Abstract: Noni (Morinda cittrifolia L.) leaves contain antraquinon, atsiri oil, saponin, tannin, alkaloid, flavonoid, polifenol, and sterol that have been proved can inhibit the growth of Candida albicans. This study was aimed to establish the minimum inhibitory concentration (MIC) of noni leaf extract against Candida abicans. This was a true experimental study with a randomized pretest-posttest control group design. We used serial dilution method with turbidimetry and spectrophotometry tests. Noni leaves were extracted by using maceration with 96% ethanol. Candida albicans fungi were obtained from Microbiology Laboratory of Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The turbidimetry test using three repetitions showed that the MIC of noni leaf extract against Candida albicans was 6.25% meanwhile the spectrophotometry test established 12.5% as the MIC of noni leaf extract. Conclusion: Minimum inhibitory concentration of noni (Morinda cittrifolia L.) leaf extract against the growth of Candida albicans was 12.5%.Keywords: noni (Morinda citrifolia L.), Candida albicans, minimum inhibitory concentration (MIC) Abstrak: Daun mengkudu (Morinda citrifolia L.) mengandung antraquinon, minyak atsiri, saponin, tannin, alkaloid, flavonoid, polifenol dan sterol yang terbukti dapat menghambat pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mendapatkan konsentrasi hambat minimum (KHM) dari ekstrak daun mengkudu terhadap Candida albicans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan dalam penelitian yaitu serial dilusi dengan pengujian turbidimetri dan spektrofotometri. Daun mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Jamur Candida albicans diambil dari stok jamur Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa pengujian turbidimetri dengan tiga kali perlakuan mendapatkan KHM pada konsentrasi 6,25% sedangkan pengujian spektrofotometri mendapatkan KHM pada konsentrasi 12,5%. Simpulan: Konsentrasi hambat minimum (KHM) ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans terdapat pada konsentrasi 12,5%.Kata kunci: mengkudu (Morinda citrifolia L.), Candia albicans, konsentrasi hambat minimum (KHM)

scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L.) terhadap Pertumbuhan Bakteri Streptococcus Mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Helen N. Sekeon ◽  
Heriyannis Homenta ◽  
Michael A. Leman
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Tooth Surface ◽  
Control Group ◽  
Control Group Design ◽  
Cariogenic Bacteria ◽  
Group Design

Abstract: Streptococcus mutans is the most common bacterium that causes dental caries due to its ability to ferment carbohydrates into acid resulting in the decreased pH on the tooth surface. Prevention of dental caries could be achieved by inhibiting the growth of cariogenic bacteria. Various efforts to control and prevent the cariogenic bacteria include the usage of herbal ingredients; one of them is gedi leaves (Abelmoschus manihot L.). These gedi leaves contain bioactive compounds such as flavonoids, alkaloids, steroids, and saponins. This study was aimed to prove that gedi leaf extract had inhibitory effect on the growth of S.mutans and to obtain the minimum inhibitory concentration (MIC) of this extract on the growth of S. mutans. This was a true experimental design with a randomized pretest-posttest control group design. Gedi leaf extract was obtained by maceration method in 96% ethanol. The results showed that gedi leaf extract had an antibacterial effect on the growth of S. mutans. We used turbidimetry, UV-Vis spectrophotometer, and two times of treatment to obtain the MIC of gedi leaf extract on Streptococcus mutans which was 6.25%. Conclusion: Gedi leaf extract could inhibit the growth of S. mutans with a MIC of 6.25%.Keywords: dental caries, gedi leaf extract (Abelmoschus manihot L.), Streptococcus mutans Abstrak: Streptococcus mutans merupakan bakteri yang paling banyak menyebabkan karies gigi karena bakteri ini berkemampuan memfermentasi karbohidrat menjadi asam yang berakibat turunnya pH pada permukaan gigi. Pencegahan karies gigi dapat dicapai dengan menghambat pertumbuhan bakteri kariogenik. Berbagai upaya dilakukan untuk mengen-dalikan dan mencegah bakteri kariogenik, antara lain dengan menggunakan bahan herbal; salah satunya yaitu tanaman gedi (Abelmoschus manihot L.). Daun gedi mengandung senyawa bioaktif antara lain flavonoid, alkaloid, steroid, dan saponin. Penelitian ini bertujuan untuk membuktikan efek inhibisi ekstrak daun gedi terhadap pertumbuhan S. mutans dan mendapatkan konsentrasi hambat minimum (KHM) ekstrak daun gedi terhadap pertumbuhan bakteri S. mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Ekstrak daun gedi dibuat dengan metode maserasi dengan menggunakan etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun gedi (Abelmoschus manihot L.) memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans. Dengan menggunakan metode turbidimetri dan spektrofotometer UV-Vis dalam 2 (dua) kali perlakuan maka diperoleh KHM ekstrak daun gedi (Abelmoschus manihot L.) terhadap bakteri Streptococcus mutans terdapat pada konsentrasi 6,25%. Simpulan: Ekstrak daun gedi dapat meghambat pertumbuhan Streptococcus mutans dengan KHM pada konsentrasi 6,25%.Kata kunci: karies gigi, ekstrak daun gedi (Abelmoschus manihot L.), Streptococcus mutans

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak daun pacar air (Impatiens balsamina L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Raymond A. Lolongan ◽  
Olivia Waworuntu ◽  
Christy N. Mintjelungan
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Microbiology Laboratory ◽  
Control Group Design ◽  
Group Design ◽  
Impatiens Balsamina ◽  
Herbal Plant

Abstract: Impatiens balsamina L. is an herbal plant which contains flavonoids that has been proven to inhibit the growth of Streptococcus mutans. This study was aimed to determine the minimum inhibitory concentration (MIC) of the balsamina leaf extract on the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-postest control group design. The method used was serial dilution and tested with the spectrophotometer and turbiditry measurements. Balsamina leaf was extracted by using maceration method with 96% ethanol. The Streptococcus mutans bacteria were obtained from pure stock bacteria at Microbiology Laboratory of Pharmacy Faculty of Mathematics, University of Sam Ratulangi. The results indicated that the minimum inhibitory concentration (MIC) of the balsamina leaf (Impatiens balsamina L.) extract on the growth of Streptococcus mutans was at a concentration of 3.125%.Keywords: balsamina leaf (Impatiens balsamina L.), Streptococcus mutans, MIC Abstrak: Pacar Air (Impatiens balsamina L.) merupakan tanaman herbal yang mengandung flavonoid yang telah terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan Randomized Pretest-Posttest Control Group Design. Metode yang digunakan dalam penelitian ini yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun Pacar air diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diperoleh dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian ini menunjukkan bahwa konsentrasi hambat minimum (KHM) ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans pada konsentrasi 3,125%. Kata kunci: daun pacar air (Impatiens balsamina L), Streptococcus mutans, KHM

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Jecklin Makolit ◽  
Olivia A. Waworuntu ◽  
Michael A. Leman
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Fruit Extract ◽  
Control Group Design ◽  
Group Design ◽  
Serial Dilution Method

Abstract: Noni fruit (Morinda cittrifolia L.) is an herbal plant that contains antibacterial substances, inter alia acubin, lasperuloside, alizarin, and some antraquinons, that have been proved to inhibit the growth of Streptococcus mutans. This study was aimed to obtain the minimum inhibitory concentration (MIC) of noni fruit extract (Morinda citrifolia L.) against the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. This study used a serial dilution method with turbidimetry and spectrophotometry tests. Noni fruit was extracted by using maceration method with 96% ethanol. Streptococcus mutans bacteria were obtained from pure stock of Microbiology Laboratory of Pharmacy Program Study, University of Sam Ratulangi Manado. The results showed that the MIC of noni fruit extract against the growth of Streptococcus mutans was at 50% of concentration. Conclusion: The minimum inhibitory concentration of Morinda citrifolia L. extract against the growth of Streptococcus mutans was 50%.Keywords: noni fruit (Morinda citrifolia L.), Streptococcus mutans, MIC Abstrak: Buah mengkudu (Morinda citrifolia, L) merupakan tanaman herbal dengan kandungan bahan berkhasiat antibakterial seperti acubin, lasperuloside, alizarin, dan beberapa zat antraquinon yang terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Buah mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa KHM ekstrak buah mengkudu terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%. Simpulan: Konsentrasi hambat minimum ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%.Kata kunci: buah mengkudu (Morinda citrifolia L.), Streptococcus mutans, KHM

scholarly journals Uji Konsentrasi Hambat Minimum (KHM) Getah Kulit Buah Pisang Goroho (Musa acuminafe L.) terhadap Pertumbuhan Staphylococcus aureus

e-GIGI ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Kleysia D. Wuon ◽  
Damajanty H.C. Pangemanan ◽  
P. S. Anindita
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Test Methods ◽  
Banana Peel ◽  
Control Group ◽  
Microbiology Laboratory ◽  
Control Group Design ◽  
Study Program ◽  
Group Design ◽  
Before And After

Abstract: Staphylococcus aureus is the most dominant cause of odontogenuic abscess, albeit, it is resistant to various types of antibiotics. The sap of goroho banana peel (Musa acuminafe L.) contains antibacteria compounds inter alia flavonoid, saponin, and tannin. This study was aimed to determine the minimal inhibitory concentration (MIC) of the sap of goroho banana peel to the growth of Staphylococcus aureus. This was a true experimental study with a randomized pretest-posttest control group design. We used serial dilution with turbidimetry and spectrophotometry as the test methods. Goroho banana was taken from Kelurahan Winangun, Kecamatan Malalayang meanwhile S. aureus bacteria were obtained from Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The result of turbidimetry after incubation showed that the tube of 50% of goroho sap looked clear. Measuring absorbance values before and after incubation using spectrophotometer UV-Vis showed that the MIC of the goroho sap was at 25%. Conclusion: The MIC of the sap of goroho banana peel (Musa acuminafe L.) to the growth of Staphylococcus aureus was at concentration of 25%.Keywords: sap of goroho banana peel (Musa acuminafe L.), S. aureus, MIC Abstrak: Staphylococcus aureus merupakan bakteri penyebab abses odontogenik yang paling dominan. Saat ini S. aureus telah resisten terhadap berbagai jenis antibiotik. Getah kulit buah pisang goroho (Musa acuminafe L.) merupakan tanaman herbal yang mengandung senyawa antibakteri, yaitu flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) getah kulit buah pisang goroho terhadap pertumbuhan S. aureus. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan yaitu metode serial dilusi dengan pengujian turbid-metri dan spektrofotometer. Pisang goroho diperoleh dari Kelurahan Winangun, Kecamatan Malalayang. Bakteri S. aureus diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian turbid-metri sesudah inkubasi menunjukkan bahwa tabung dengan konsentrasi getah kulit buah pisang goroho 50% terlihat mulai jernih. Pengukuran nilai absorbansi sebelum dan sesudah inkubasi menggunakan spektrofotometer UV-Vis menunjukkan bahwa KHM getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri S. aureus terdapat pada konsentrasi 25%. Simpulan: Konsentrasi hambat minimum getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri S. aureus terdapat pada konsentrasi 25%Kata kunci: getah kulit buah pisang goroho (M. acuminafe), S. aureus, KHM

scholarly journals Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

2020 ◽  
Vol 8 (3) ◽  
pp. 801
Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Soft Tissues ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Alternative Medicines ◽  
Pharmaceutical Properties

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

scholarly journals UJI MINIMUM INHIBITORY CONCENTRATION (MIC) EKSTRAK RUMPUT LAUT (Eucheuma cottonii) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus mutans

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Heryudi J. J. Soelama ◽  
Billy J. Kepel ◽  
Krista V. Siagian
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Calibration Method ◽  
Bactericidal Effect ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Eucheuma Cottonii

Abstract: Dental caries is an oral cavity disease which becomes a health problem in Indonesia. Bacteria that play some important role in caries formation are Streptococcus mutans. One of the nature material that can be used to restrain Streptococcus mutans is seaweed Eucheuma cottonii. This study aimed to determine the Minimum Inhibitory Concentration (MIC) of seaweed Eucheuma cottonii extract against Streptococcus mutans. This was an experimental laboratory study with a post test only control group design. MIC test was done by serial dilution, turbidimetry, and spectrophotometry methods, as well as bactericidal test using aa calibration method. Eucheuma cottonii were taken from the sea around Naen island and then were extracted with maceration method using ethanol 96%. The results showed that the MIC of Echeuma cottonii extract was in the concentration of 6.25% against Streptococcus mutans. Growth inhibition of Streptococcus mutans was significant in the concentration of 6.25%, 12.5%, 25%, 50%, and 100%. MIC of Eucheuma cottonii extract was 6.25% but the MBC or bactericidal effect was not found.The higher concentration of Eucheuma cottonii used, the greater ability to inhibit the growth of Streptococcus mutans. Conclusion: Seaweed Echeuma cottonii extract has bacteriostatic abilities but do not have bactericide abilities to against Streptococcus mutans.Keywords: streptococcus mutans, mic, eucheuma cottonii, alternative medicine, coastal communitiesAbstrak: Karies gigi merupakan penyakit rongga mulut yang paling banyak diderita masyarakat dan menjadi salah satu masalah kesehatan di Indonesia. Bakteri yang paling berperan dalam pembentukan karies adalah Streptococcus mutans. Cara alternatif untuk menanggulangi Streptococcus mutans adalah dengan menggunakan bahan alami yaitu rumput laut (Eucheuma cottonii).Tujuan penelitian ini adalah untuk mengetahui Minimum Inhibitory Concentration (MIC) rumput laut (Eucheuma cottonii) terhadap Streptococcus mutans. Penelitian ini merupakan penelitian eksperimental laboratorium menggunakan desain post-test only control group design. Uji MIC dilakukan dengan metode serial dilusi, turbidimetri dan spektrofotometri, serta uji kalibrasi untuk uji sifat bakteriosid. Sampel diambil dari perairan pulau Naen kemudian diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Hasil penelitian menunjukkan bahwa MIC ekstrak rumput laut (Eucheuma cottonii) pada konsentrasi 6.25% terhadap Streptococcus mutans. Hasil analisis dan pengolahan data menunjukkan daya hambat ekstrak rumput laut (Eucheuma cottonii) terhadap pertumbuhan Streptococcus mutans yang paling signifikan ialah konsentrasi 6,25%, 12,5%, 25%, 50%, dan 100%. MIC ekstrak rumput laut (Eucheuma cottonii) 6,25% sedangkan MBC atau sifat bakteriosid tidak ditemukan. Semakin tinggi konsentrasi ekstrak rumput laut (Eucheuma cottonii) yang digunakan, maka semakin besar kemampuan menghambat pertumbuhan Streptococcus mutans. Simpulan: Ekstrak rumput laut (Eucheuma cottonii) memiliki sifat bakteriostatik namun tidak memiliki sifat bakteriosid terhadap Streptococcus mutans.Kata kunci: streptococcus mutans, mic, eucheuma cottonii, pengobatan alternatif, masyarakat pesisir pantai

scholarly journals EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum L.) ON GLUCOSYLTRANSFERASE ENZYME OF Streptococcus mutans

2020 ◽  
Vol 5 (2) ◽  
pp. 110
Author(s):  
Ferdy Juliannor Fajar ◽  
Deby Kania Tri Putri ◽  
Bayu Indra Sukmana
Keyword(s):  
Enzyme Activity ◽  
Streptococcus Mutans ◽  
Cell Walls ◽  
Leaf Extract ◽  
Plaque Formation ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Melastoma Malabathricum ◽  
Post Test

ABSTRACTBackground: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%, 20%, and 25% concentrations to determine the activity of S. mutans GTF enzyme. Results: The results showed that Karamunting leaf extract with 20% concentration was able to decrease the activity of Glucosyltransferase enzyme in Streptococcus mutans. Conclusion: Karamunting leaf extract affects Streptococcus mutans Glucosyltransferase enzyme activity. Keywords: Chlorhexidine 0,12%, Glucosiltransferase, Melastoma malabathricum L., Streptococcus mutans.

scholarly journals PENERAPAN METODE SQ3R DAN METODE PQ3R TERHADAP KETERAMPILAN MEMBACA PADA MAHASISWA

2018 ◽  
Vol 2 (1) ◽  
pp. 69-80
Author(s):  
Rini Agustina ◽  
Hariyadi Hariyadi
Keyword(s):  
Data Analysis ◽  
Random Sampling ◽  
Design Research ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Study Program ◽  
Average Percentage ◽  
Group Design ◽  
Class C

This study aims to describe the effect of application of SQ3R method and PQ3R method to students' reading skill. This research uses experimental method, Quasi Experimental Design research form with Nonequivalent Control Group Design design. Population in this research is all student of Study Program of Language and Literature of Indonesia semester 6 which amounted to 243. The sample used is class C Morning and class C afternoon obtained by simple random sampling technique. The technique used is the measurement with the tool in the form of reading test in the form of multiple choice which amounted to 30 items. Data analysis technique used in this research is anava 2 lane. Based on the result of data analysis, it is known that the application of SQ3R method and PQ3R method is good with the details as follows: (1) The average value before given treatment 20,476 with enough category; (2) the average application of the SQ3R method is 22.097 with good category; (3) the average percentage of PQ3R method is 22,045 with good category.   Keywords: method SQ3R, PQ3R method, read     Abstrak Penelitian ini bertujuan untuk mendeskripsikan pengaruh penerapan metode SQ3R dan metode PQ3R terhadap keterampilan membaca mahasiswa. Penelitian ini menggunakan metode eksperimen, bentuk penelitian  Quasi Eksperimental Design dengan rancangan Nonequivalent Control Group Design. Populasi dalam penelitian ini adalah seluruh mahasiswa Program Studi Pendidikan Bahasa dan Sastra Indonesia semester 6 yang berjumlah 243. Sampel yang digunakan yaitu kelas C Pagi dan kelas C sore yang diperoleh dengan Teknik simple random sampling. Teknik yang digunakan yaitu pengukuran dengan alatnya berupa tes membaca berupa pilihan ganda yang berjumlah 30 butir soal. Teknik analisis data yang digunakan dalam penelitian ini adalah anava 2 jalur. Berdasarkan hasil analisis data, diketahui bahwa penerapan metode SQ3R dan Metode PQ3R tergolong baik dengan rincian sebagai berikut: (1) Nilai rata-rata sebelum diberikan perlakuan 20,476 dengan kategori cukup; (2)  rata-rata penerapan metode SQ3R sebesar 22,097 dengan katergori baik; (3) persentase rata-rata metode PQ3R sebesar 22,045 denga kategori baik.   Kata kunci: metode SQ3R, metode PQ3R, membaca

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