scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L. Medik) terhadap Pertumbuhan Streptococcus mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Benedicta N. D. Rori ◽  
Johanna A. Khoman ◽  
Aurelia S. R. Supit
Keyword(s):  
Dental Caries ◽  
Minimal Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Group Design ◽  
Extract Concentration

Abstract: The main problem in dental health is dental caries that occurs due to the fermentation by Streptococcus mutans bacteria. Gedi leaf (Abelmoschus manihot L. Medical) is one of the common plants in Northern Celebes that contains antimicrobial compounds namely flavonoid, alkaloid, steroid, and saponin; all of them have been proved to inhibit the growth of S. mutans. This study was aimed to determine the minimal inhibitory concentration (MIC) of gedi leaf extract (Abelmoschus manihot L. Medical) to S. mutans growth. This was a true experimental study with a randomized pretest-posttest control group design. The method used in this study was serial dilution method with turbidimetry and spectrophotometry. Samples of gedi leaves were obtained at Paal 2 Manado, and were extracted with maceration method using ethanol 96%. S. mutans bacteria were obtained from the pure bacterial stock at Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Science University of Sam Ratulangi Manado. The turbidimetry test showed that the tube content became clearer at 25% of extract concentration. Moreover, the UV-Vis spectro-photometer showed a decrease of OD value for the first time at 25% of extract concentration. Conclusion: The minimal inhibitory concentration (MIC) of gedi leaf extract (Abelmoschus manihot L. Medical) to Streptococcus mutans growth was at concentration of 25%.Keywords: gedi leaf (Abelmoschus manihot L. Medical), Streptococcus mutans, MIC, dental caries Abstrak: Masalah utama dalam kesehatan gigi ialah karies gigi yang terbentuk karena proses peragian oleh bakteri Streptococcus mutans. Daun gedi (Abelmoschus manihot L. Medik) merupakan salah satu tanaman khas daerah Sulawesi Utara yang mengandung senyawa antimikroba berupa flavonoid, alkaloid, steroid, dan saponin yang telah terbukti memiliki efek antibakteri terhadap pertumbuhan S. mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) ekstrak daun gedi (Abelmoschus manihot L. Medik) terhadap pertumbuhan S. mutans. Jenis penelitian ialah eksperimental murni dengan pretest-posttest control group design. Metode yang digunakan dalam penelitian ini yaitu metode serial dilusi dengan pengujian turbidimetri dan spektrofotometri. Daun gedi diperoleh dari Kecamatan Paal 2 Manado, dan diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri S. mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil pengujian turbidimetri memperlihatkan bahwa larutan dalam tabung terlihat mulai jernih pada konsentrasi ekstrak 25%. Pada pengujian spektrofotometer UV-Vis terlihat penurunan nilai OD pertama kali pada konsentrasi 25%. Simpulan: Konsentrasi hambat minimum ekstrak daun gedi (Abelmoschus manihot L. Medik) terhadap pertumbuhan Streptococcus mutans terdapat pada konsentrasi 25%.Kata kunci: daun gedi (Abelmoschus manihot L. Medik), Streptococcus mutans, KHM, karies

Uji konsentrasi hambat minimum (KHM) ekstrak daun binahong (Anredera cordifolia Steenis) sebagai antibakteri terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Klaudya E. Warokka ◽  
Jane Wuisan ◽  
Juliatri .
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Test Methods ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Study Program ◽  
Group Design

Abstract: Inadequate oral and dental hygiene can cause plaques containing various kinds of bacteria; one of them is Streptococcus mutans which is the main cause of dental caries. Binahong leaf (Anredera cordifolia Steenis) is a medicinal herb that contains antibacterial compounds namely flavonoids, alkonoid, terpanoid, and saponins. This study aimed to determine the minimal inhibitory concentration (MIC) of binahong leaf extract to the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. The method used in this study was serial dilution method with turbidimetry and spectrophotometry as the test methods. Binahong leaves were taken from Tempok village and were extracted with maceration method using ethanol 96%. Streptococcus mutans bacteria were obtained from a pure bacterial stock in Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Science University of Sam Ratulangi Manado. The result showed that the minimal inhibitory concentration (MIC) of binahong leaf extract (Anredera cordifolia steenis) to the growth of Streptococcus mutans was 6.25%.Keywords: Streptococcus mutans, binahong leaf, MIC, tooth cariesAbstrak: Kebersihan gigi dan mulut yang buruk dapat menyebabkan terbentuknya plak yang mengandung berbagai macam bakteri, salah satu diantaranya Streptococcus mutans yang menjadi penyebab utama terjadinya karies gigi. Daun binahong (Anredera cordifolia steenis) merupakan tanaman herbal yang mengandung senyawa antibakteri yaitu flavonoid, alkonoid, terpanoid dan saponin. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari daun binahong terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Penelitian ini menggunakan metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun binahong diperoleh di desa Tempok, dan diekstraksi dengan metode maserasi menggunakan etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan bahwa KHM daun binahong terhadap pertumbuhan streptococcus mutans ialah pada konsentrasi 6,25%.Kata kunci: Streptococcus mutans, daun binahong, KHM, karies gigi

scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L.) terhadap Pertumbuhan Bakteri Streptococcus Mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Helen N. Sekeon ◽  
Heriyannis Homenta ◽  
Michael A. Leman
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Tooth Surface ◽  
Control Group ◽  
Control Group Design ◽  
Cariogenic Bacteria ◽  
Group Design

Abstract: Streptococcus mutans is the most common bacterium that causes dental caries due to its ability to ferment carbohydrates into acid resulting in the decreased pH on the tooth surface. Prevention of dental caries could be achieved by inhibiting the growth of cariogenic bacteria. Various efforts to control and prevent the cariogenic bacteria include the usage of herbal ingredients; one of them is gedi leaves (Abelmoschus manihot L.). These gedi leaves contain bioactive compounds such as flavonoids, alkaloids, steroids, and saponins. This study was aimed to prove that gedi leaf extract had inhibitory effect on the growth of S.mutans and to obtain the minimum inhibitory concentration (MIC) of this extract on the growth of S. mutans. This was a true experimental design with a randomized pretest-posttest control group design. Gedi leaf extract was obtained by maceration method in 96% ethanol. The results showed that gedi leaf extract had an antibacterial effect on the growth of S. mutans. We used turbidimetry, UV-Vis spectrophotometer, and two times of treatment to obtain the MIC of gedi leaf extract on Streptococcus mutans which was 6.25%. Conclusion: Gedi leaf extract could inhibit the growth of S. mutans with a MIC of 6.25%.Keywords: dental caries, gedi leaf extract (Abelmoschus manihot L.), Streptococcus mutans Abstrak: Streptococcus mutans merupakan bakteri yang paling banyak menyebabkan karies gigi karena bakteri ini berkemampuan memfermentasi karbohidrat menjadi asam yang berakibat turunnya pH pada permukaan gigi. Pencegahan karies gigi dapat dicapai dengan menghambat pertumbuhan bakteri kariogenik. Berbagai upaya dilakukan untuk mengen-dalikan dan mencegah bakteri kariogenik, antara lain dengan menggunakan bahan herbal; salah satunya yaitu tanaman gedi (Abelmoschus manihot L.). Daun gedi mengandung senyawa bioaktif antara lain flavonoid, alkaloid, steroid, dan saponin. Penelitian ini bertujuan untuk membuktikan efek inhibisi ekstrak daun gedi terhadap pertumbuhan S. mutans dan mendapatkan konsentrasi hambat minimum (KHM) ekstrak daun gedi terhadap pertumbuhan bakteri S. mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Ekstrak daun gedi dibuat dengan metode maserasi dengan menggunakan etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun gedi (Abelmoschus manihot L.) memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans. Dengan menggunakan metode turbidimetri dan spektrofotometer UV-Vis dalam 2 (dua) kali perlakuan maka diperoleh KHM ekstrak daun gedi (Abelmoschus manihot L.) terhadap bakteri Streptococcus mutans terdapat pada konsentrasi 6,25%. Simpulan: Ekstrak daun gedi dapat meghambat pertumbuhan Streptococcus mutans dengan KHM pada konsentrasi 6,25%.Kata kunci: karies gigi, ekstrak daun gedi (Abelmoschus manihot L.), Streptococcus mutans

scholarly journals Uji Konsentrasi Hambat Minimum (KHM) Ekstrak Daun Mengkudu (Morinda citrifolia L.) terhadap Candida albicans Secara In Vitro

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Lisa Ramschie ◽  
Pieter L. Suling ◽  
Krista V. Siagian
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Group Design

Abstract: Noni (Morinda cittrifolia L.) leaves contain antraquinon, atsiri oil, saponin, tannin, alkaloid, flavonoid, polifenol, and sterol that have been proved can inhibit the growth of Candida albicans. This study was aimed to establish the minimum inhibitory concentration (MIC) of noni leaf extract against Candida abicans. This was a true experimental study with a randomized pretest-posttest control group design. We used serial dilution method with turbidimetry and spectrophotometry tests. Noni leaves were extracted by using maceration with 96% ethanol. Candida albicans fungi were obtained from Microbiology Laboratory of Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The turbidimetry test using three repetitions showed that the MIC of noni leaf extract against Candida albicans was 6.25% meanwhile the spectrophotometry test established 12.5% as the MIC of noni leaf extract. Conclusion: Minimum inhibitory concentration of noni (Morinda cittrifolia L.) leaf extract against the growth of Candida albicans was 12.5%.Keywords: noni (Morinda citrifolia L.), Candida albicans, minimum inhibitory concentration (MIC) Abstrak: Daun mengkudu (Morinda citrifolia L.) mengandung antraquinon, minyak atsiri, saponin, tannin, alkaloid, flavonoid, polifenol dan sterol yang terbukti dapat menghambat pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mendapatkan konsentrasi hambat minimum (KHM) dari ekstrak daun mengkudu terhadap Candida albicans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan dalam penelitian yaitu serial dilusi dengan pengujian turbidimetri dan spektrofotometri. Daun mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Jamur Candida albicans diambil dari stok jamur Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa pengujian turbidimetri dengan tiga kali perlakuan mendapatkan KHM pada konsentrasi 6,25% sedangkan pengujian spektrofotometri mendapatkan KHM pada konsentrasi 12,5%. Simpulan: Konsentrasi hambat minimum (KHM) ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans terdapat pada konsentrasi 12,5%.Kata kunci: mengkudu (Morinda citrifolia L.), Candia albicans, konsentrasi hambat minimum (KHM)

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak daun pacar air (Impatiens balsamina L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Raymond A. Lolongan ◽  
Olivia Waworuntu ◽  
Christy N. Mintjelungan
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Microbiology Laboratory ◽  
Control Group Design ◽  
Group Design ◽  
Impatiens Balsamina ◽  
Herbal Plant

Abstract: Impatiens balsamina L. is an herbal plant which contains flavonoids that has been proven to inhibit the growth of Streptococcus mutans. This study was aimed to determine the minimum inhibitory concentration (MIC) of the balsamina leaf extract on the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-postest control group design. The method used was serial dilution and tested with the spectrophotometer and turbiditry measurements. Balsamina leaf was extracted by using maceration method with 96% ethanol. The Streptococcus mutans bacteria were obtained from pure stock bacteria at Microbiology Laboratory of Pharmacy Faculty of Mathematics, University of Sam Ratulangi. The results indicated that the minimum inhibitory concentration (MIC) of the balsamina leaf (Impatiens balsamina L.) extract on the growth of Streptococcus mutans was at a concentration of 3.125%.Keywords: balsamina leaf (Impatiens balsamina L.), Streptococcus mutans, MIC Abstrak: Pacar Air (Impatiens balsamina L.) merupakan tanaman herbal yang mengandung flavonoid yang telah terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan Randomized Pretest-Posttest Control Group Design. Metode yang digunakan dalam penelitian ini yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun Pacar air diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diperoleh dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian ini menunjukkan bahwa konsentrasi hambat minimum (KHM) ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans pada konsentrasi 3,125%. Kata kunci: daun pacar air (Impatiens balsamina L), Streptococcus mutans, KHM

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Jecklin Makolit ◽  
Olivia A. Waworuntu ◽  
Michael A. Leman
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Fruit Extract ◽  
Control Group Design ◽  
Group Design ◽  
Serial Dilution Method

Abstract: Noni fruit (Morinda cittrifolia L.) is an herbal plant that contains antibacterial substances, inter alia acubin, lasperuloside, alizarin, and some antraquinons, that have been proved to inhibit the growth of Streptococcus mutans. This study was aimed to obtain the minimum inhibitory concentration (MIC) of noni fruit extract (Morinda citrifolia L.) against the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. This study used a serial dilution method with turbidimetry and spectrophotometry tests. Noni fruit was extracted by using maceration method with 96% ethanol. Streptococcus mutans bacteria were obtained from pure stock of Microbiology Laboratory of Pharmacy Program Study, University of Sam Ratulangi Manado. The results showed that the MIC of noni fruit extract against the growth of Streptococcus mutans was at 50% of concentration. Conclusion: The minimum inhibitory concentration of Morinda citrifolia L. extract against the growth of Streptococcus mutans was 50%.Keywords: noni fruit (Morinda citrifolia L.), Streptococcus mutans, MIC Abstrak: Buah mengkudu (Morinda citrifolia, L) merupakan tanaman herbal dengan kandungan bahan berkhasiat antibakterial seperti acubin, lasperuloside, alizarin, dan beberapa zat antraquinon yang terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Buah mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa KHM ekstrak buah mengkudu terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%. Simpulan: Konsentrasi hambat minimum ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Streptococcus mutans pada konsentrasi 50%.Kata kunci: buah mengkudu (Morinda citrifolia L.), Streptococcus mutans, KHM

scholarly journals Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

2020 ◽  
Vol 8 (3) ◽  
pp. 801
Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Soft Tissues ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Alternative Medicines ◽  
Pharmaceutical Properties

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

scholarly journals Effectivity of Jatropha multifida L. Leaves Extract as Antibacterial on Streptococcus mutans using In Vitro Testing Methods

2021 ◽  
Vol 7 (2) ◽  
pp. 415-421
Author(s):  
Listyaning Kartiko Kinasih ◽  
Idamawati Nababan ◽  
Suci Erawati ◽  
Rouli Natasia M Simanjuntak
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Control Group ◽  
Entire Body ◽  
Body Parts ◽  
Control Group Design ◽  
The Individual ◽  
Jatropha Multifida

Intoduction: Health is the condition of the entire body and body parts of an individual who is free from disease both physically, mentally and socially, thus enabling the individual to be able to carry out daily activities more productively. However, many people neglect their own health, especially in the field of dental and oral health. Caries is one of the most common oral and dental problems. The prevalence of dental caries in Indonesia is high, namely 88.8%. Dental caries is caused by Streptococcus mutans bacteria which plays a role in the conversion of sucrose to lactic acid. Infection due to Streptococcus mutans bacteria can be treated by taking antibiotics. Apart from drugs, antibiotics can also be obtained from natural ingredients, one of which is the tintir castor plant (Jatropha multifida Linn) or commonly known as the betadine. Objective: To see the effect of the antibacterial content of tintir leaves against Streptococcus mutans bacteria and to determine the differences in the antibacterial effect of betadine leaf extract (Jatropha multifida L.) from a concentration of 25%, 50%, 75%, and 100% against Streptococcus mutans. Methods: The type of research used in this study was a laboratory experimental study with a post-test-only control group design as the research design and to test the sensitivity of bacteria using the paper disc diffusion test. Data analysis using One-Way ANOVA. Result: The results of this study indicate the effect of tintir castor leaf extract (Jatropha multifida L.) in inhibiting the growth of Streptococcus mutans bacteria.

scholarly journals Bioviability of Mangrove Leaves Extract (Sonneratia alba) on Fibroblast BHK-21 Cell Culture

DENTA ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 1
Author(s):  
Arlita Gladys Tricia Charyadie ◽  
Aprilia Aprilia ◽  
Widyastuti Widyastuti
Keyword(s):  
Cell Culture ◽  
Dental Caries ◽  
Cell Viability ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Test Group ◽  
Control Group ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Sonneratia Alba

<p><strong>Background:</strong> Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba has<br />been proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. <strong>Purpose:</strong> The aim of this study is to determine the cell viability of<br />fibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. <strong>Materials and Methods:</strong> The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. <strong>Results:</strong> Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). <strong>Conclusion:</strong> In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells.</p><p> </p><p><strong>Keywords:</strong> Dental caries, Sonneratia alba, fibroblast, viability, cell culture</p><p><strong><br /></strong></p><p><strong>Correspondence:</strong> Aprilia, Department of Conservation, Faculty of Dentistry, Hang Tuah Univesity, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <span style="text-decoration: underline;">[email protected]</span></p>

scholarly journals UJI MINIMUM INHIBITORY CONCENTRATION (MIC) EKSTRAK RUMPUT LAUT (Eucheuma cottonii) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus mutans

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Heryudi J. J. Soelama ◽  
Billy J. Kepel ◽  
Krista V. Siagian
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Calibration Method ◽  
Bactericidal Effect ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Eucheuma Cottonii

Abstract: Dental caries is an oral cavity disease which becomes a health problem in Indonesia. Bacteria that play some important role in caries formation are Streptococcus mutans. One of the nature material that can be used to restrain Streptococcus mutans is seaweed Eucheuma cottonii. This study aimed to determine the Minimum Inhibitory Concentration (MIC) of seaweed Eucheuma cottonii extract against Streptococcus mutans. This was an experimental laboratory study with a post test only control group design. MIC test was done by serial dilution, turbidimetry, and spectrophotometry methods, as well as bactericidal test using aa calibration method. Eucheuma cottonii were taken from the sea around Naen island and then were extracted with maceration method using ethanol 96%. The results showed that the MIC of Echeuma cottonii extract was in the concentration of 6.25% against Streptococcus mutans. Growth inhibition of Streptococcus mutans was significant in the concentration of 6.25%, 12.5%, 25%, 50%, and 100%. MIC of Eucheuma cottonii extract was 6.25% but the MBC or bactericidal effect was not found.The higher concentration of Eucheuma cottonii used, the greater ability to inhibit the growth of Streptococcus mutans. Conclusion: Seaweed Echeuma cottonii extract has bacteriostatic abilities but do not have bactericide abilities to against Streptococcus mutans.Keywords: streptococcus mutans, mic, eucheuma cottonii, alternative medicine, coastal communitiesAbstrak: Karies gigi merupakan penyakit rongga mulut yang paling banyak diderita masyarakat dan menjadi salah satu masalah kesehatan di Indonesia. Bakteri yang paling berperan dalam pembentukan karies adalah Streptococcus mutans. Cara alternatif untuk menanggulangi Streptococcus mutans adalah dengan menggunakan bahan alami yaitu rumput laut (Eucheuma cottonii).Tujuan penelitian ini adalah untuk mengetahui Minimum Inhibitory Concentration (MIC) rumput laut (Eucheuma cottonii) terhadap Streptococcus mutans. Penelitian ini merupakan penelitian eksperimental laboratorium menggunakan desain post-test only control group design. Uji MIC dilakukan dengan metode serial dilusi, turbidimetri dan spektrofotometri, serta uji kalibrasi untuk uji sifat bakteriosid. Sampel diambil dari perairan pulau Naen kemudian diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Hasil penelitian menunjukkan bahwa MIC ekstrak rumput laut (Eucheuma cottonii) pada konsentrasi 6.25% terhadap Streptococcus mutans. Hasil analisis dan pengolahan data menunjukkan daya hambat ekstrak rumput laut (Eucheuma cottonii) terhadap pertumbuhan Streptococcus mutans yang paling signifikan ialah konsentrasi 6,25%, 12,5%, 25%, 50%, dan 100%. MIC ekstrak rumput laut (Eucheuma cottonii) 6,25% sedangkan MBC atau sifat bakteriosid tidak ditemukan. Semakin tinggi konsentrasi ekstrak rumput laut (Eucheuma cottonii) yang digunakan, maka semakin besar kemampuan menghambat pertumbuhan Streptococcus mutans. Simpulan: Ekstrak rumput laut (Eucheuma cottonii) memiliki sifat bakteriostatik namun tidak memiliki sifat bakteriosid terhadap Streptococcus mutans.Kata kunci: streptococcus mutans, mic, eucheuma cottonii, pengobatan alternatif, masyarakat pesisir pantai

scholarly journals Artocarpus altilis Extracts as Liquid Electric Mosquito Repellent Against Aedes aegypti

2018 ◽  
Vol 7 (2) ◽  
pp. 84-88
Author(s):  
Ernawati Ernawati ◽  
Widya Hary Cahyati
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Probit Analysis ◽  
Control Group ◽  
Mosquito Repellent ◽  
Control Group Design ◽  
Artocarpus Altilis ◽  
Group Design ◽  
Extract Concentration ◽  
Mosquito Mortality

ABSTRACT Aedes aegypti is the main vector of dengue virus carriers. One alternative to deal with these problem is by using insecticides derived from the leaves of breadfruit (Artocarous altilis) with the method of liquid electric. The research was purely experimental, with a posttest-only control group design with six variations of extract concentration of 9%, 13%, 20%, 30%, 44%, and 66% with 3 repetitions. The result of Kruskal Wallis test showed that there was a difference of the average number of Aedes aegypti mosquito mortality on various concentration of breadfruit leaf extract, that indicated by significance or probability value of 0.008 <0.05. The deadliest concentration of breadfruit leaf was 66% concentration with mosquito mortality of 83.3% from all mosquito test. The probit analysis obtained LC50 at concentration of 17.850% and LC90 at concentration of 98.348%.   Keywords: Aedes aegypti, Breadfruit Leaf Extract, Liquid Electric   ABSTRAK Aedes aegypti merupakan nyamuk yang menjadi vektor utama pembawa virus dengue. Salah satu alternatif lain untuk menangani permasalahan tersebut yaitu dengan menggunakan insektisida yang berasal dari tumbuhan atau  nabati, yaitu daun sukun (Artocarous altilis) dengan metode elektrik cair. Jenis penelitian ini adalah eksperimen murni, dengan rancangan posttest only control group design dengan enam variasi konsentrasi ekstrak sebesar 9%, 13%, 20%, 30%, 44%, dan 66% dengan masing-masing 3 kali pengulangan. Hasil uji Kruskal Wallis menunjukkan bahwa ada perbedaan rata-rata jumlah kematian nyamuk Aedes aegypti pada berbagai konsentrasi ekstrak daun sukun, ditunjukkan dengan nilai signifikansi atau probabilitas adalah 0,008 < 0,05. Konsentrasi ekstrak daun sukun yang paling banyak mematikan nyamuk yaitu konsentrasi 66% dengan kematian nyamuk 83,3 % dari keseluruhan nyamuk. Uji analisis probit didapatkan LC50 pada konsentrasi 17.850% dan LC90 pada konsentrasi 98.348%.   Kata kunci: Aedes aegypti, Ekstrak Daun Sukun, Elektrik Cair

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