scholarly journals EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum L.) ON GLUCOSYLTRANSFERASE ENZYME OF Streptococcus mutans

2020 ◽  
Vol 5 (2) ◽  
pp. 110
Author(s):  
Ferdy Juliannor Fajar ◽  
Deby Kania Tri Putri ◽  
Bayu Indra Sukmana
Keyword(s):  
Enzyme Activity ◽  
Streptococcus Mutans ◽  
Cell Walls ◽  
Leaf Extract ◽  
Plaque Formation ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Melastoma Malabathricum ◽  
Post Test

ABSTRACTBackground: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%, 20%, and 25% concentrations to determine the activity of S. mutans GTF enzyme. Results: The results showed that Karamunting leaf extract with 20% concentration was able to decrease the activity of Glucosyltransferase enzyme in Streptococcus mutans. Conclusion: Karamunting leaf extract affects Streptococcus mutans Glucosyltransferase enzyme activity. Keywords: Chlorhexidine 0,12%, Glucosiltransferase, Melastoma malabathricum L., Streptococcus mutans.

scholarly journals UJI EFEK ANTI BAKTERI EKSTRAK BUNGA CENGKEH TERHADAP BAKTERI Streptococcus mutans SECARA IN VITRO

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Antibacterial Effects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Clove Extract ◽  
Post Hoc ◽  
One Way Anova

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans

scholarly journals Uji Konsentrasi Hambat Minimum Ekstrak Daun Gedi (Abelmoschus manihot L.) terhadap Pertumbuhan Bakteri Streptococcus Mutans

e-GIGI ◽  
2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Helen N. Sekeon ◽  
Heriyannis Homenta ◽  
Michael A. Leman
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Tooth Surface ◽  
Control Group ◽  
Control Group Design ◽  
Cariogenic Bacteria ◽  
Group Design

Abstract: Streptococcus mutans is the most common bacterium that causes dental caries due to its ability to ferment carbohydrates into acid resulting in the decreased pH on the tooth surface. Prevention of dental caries could be achieved by inhibiting the growth of cariogenic bacteria. Various efforts to control and prevent the cariogenic bacteria include the usage of herbal ingredients; one of them is gedi leaves (Abelmoschus manihot L.). These gedi leaves contain bioactive compounds such as flavonoids, alkaloids, steroids, and saponins. This study was aimed to prove that gedi leaf extract had inhibitory effect on the growth of S.mutans and to obtain the minimum inhibitory concentration (MIC) of this extract on the growth of S. mutans. This was a true experimental design with a randomized pretest-posttest control group design. Gedi leaf extract was obtained by maceration method in 96% ethanol. The results showed that gedi leaf extract had an antibacterial effect on the growth of S. mutans. We used turbidimetry, UV-Vis spectrophotometer, and two times of treatment to obtain the MIC of gedi leaf extract on Streptococcus mutans which was 6.25%. Conclusion: Gedi leaf extract could inhibit the growth of S. mutans with a MIC of 6.25%.Keywords: dental caries, gedi leaf extract (Abelmoschus manihot L.), Streptococcus mutans Abstrak: Streptococcus mutans merupakan bakteri yang paling banyak menyebabkan karies gigi karena bakteri ini berkemampuan memfermentasi karbohidrat menjadi asam yang berakibat turunnya pH pada permukaan gigi. Pencegahan karies gigi dapat dicapai dengan menghambat pertumbuhan bakteri kariogenik. Berbagai upaya dilakukan untuk mengen-dalikan dan mencegah bakteri kariogenik, antara lain dengan menggunakan bahan herbal; salah satunya yaitu tanaman gedi (Abelmoschus manihot L.). Daun gedi mengandung senyawa bioaktif antara lain flavonoid, alkaloid, steroid, dan saponin. Penelitian ini bertujuan untuk membuktikan efek inhibisi ekstrak daun gedi terhadap pertumbuhan S. mutans dan mendapatkan konsentrasi hambat minimum (KHM) ekstrak daun gedi terhadap pertumbuhan bakteri S. mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Ekstrak daun gedi dibuat dengan metode maserasi dengan menggunakan etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak daun gedi (Abelmoschus manihot L.) memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans. Dengan menggunakan metode turbidimetri dan spektrofotometer UV-Vis dalam 2 (dua) kali perlakuan maka diperoleh KHM ekstrak daun gedi (Abelmoschus manihot L.) terhadap bakteri Streptococcus mutans terdapat pada konsentrasi 6,25%. Simpulan: Ekstrak daun gedi dapat meghambat pertumbuhan Streptococcus mutans dengan KHM pada konsentrasi 6,25%.Kata kunci: karies gigi, ekstrak daun gedi (Abelmoschus manihot L.), Streptococcus mutans

Uji konsentrasi hambat minimum (KHM) ekstrak daun binahong (Anredera cordifolia Steenis) sebagai antibakteri terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Klaudya E. Warokka ◽  
Jane Wuisan ◽  
Juliatri .
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Test Methods ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Study Program ◽  
Group Design

Abstract: Inadequate oral and dental hygiene can cause plaques containing various kinds of bacteria; one of them is Streptococcus mutans which is the main cause of dental caries. Binahong leaf (Anredera cordifolia Steenis) is a medicinal herb that contains antibacterial compounds namely flavonoids, alkonoid, terpanoid, and saponins. This study aimed to determine the minimal inhibitory concentration (MIC) of binahong leaf extract to the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-posttest control group design. The method used in this study was serial dilution method with turbidimetry and spectrophotometry as the test methods. Binahong leaves were taken from Tempok village and were extracted with maceration method using ethanol 96%. Streptococcus mutans bacteria were obtained from a pure bacterial stock in Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Science University of Sam Ratulangi Manado. The result showed that the minimal inhibitory concentration (MIC) of binahong leaf extract (Anredera cordifolia steenis) to the growth of Streptococcus mutans was 6.25%.Keywords: Streptococcus mutans, binahong leaf, MIC, tooth cariesAbstrak: Kebersihan gigi dan mulut yang buruk dapat menyebabkan terbentuknya plak yang mengandung berbagai macam bakteri, salah satu diantaranya Streptococcus mutans yang menjadi penyebab utama terjadinya karies gigi. Daun binahong (Anredera cordifolia steenis) merupakan tanaman herbal yang mengandung senyawa antibakteri yaitu flavonoid, alkonoid, terpanoid dan saponin. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari daun binahong terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ini ialah eksperimental murni dengan randomized pretest-posttest control group design. Penelitian ini menggunakan metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun binahong diperoleh di desa Tempok, dan diekstraksi dengan metode maserasi menggunakan etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan bahwa KHM daun binahong terhadap pertumbuhan streptococcus mutans ialah pada konsentrasi 6,25%.Kata kunci: Streptococcus mutans, daun binahong, KHM, karies gigi

scholarly journals Uji daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Anggriana H. Bujung ◽  
Heriyannis Homenta ◽  
Johanna A. Khoman
Keyword(s):  
Streptococcus Mutans ◽  
Inhibitory Effect ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Paper Disk ◽  
Post Test ◽  
Avocado Seed

Abstract: In addition to daily consumption, avocado is also used to treat oral diseases. Scientific studies showed that avocado seed contained flavonoids, tannins, and alkaloid which were expected to inhibit bacterial growth. This study was aimed to obtain the bacterial inhibitory effect of avocado seed (Persea americana Mill.) extract on Streptococcus mutans as the primary cause of dental caries. This was a true experimental study with the post test only control group design. This study used a modified Kirby-Bauer method with paper disk. The positive control was erythromycin and the negative control was aquadest. Avocado seeds were extracted by using maceration method with 96% ethanol. The Streptococcus mutans bacteria were obtained from pure stock of Microbiology Laboratory of Pharmacy Study Program University of Sam Ratulangi Manado. The results showed that the mean diameter of inhibition zone of avocado seed extract was 21.8 mm which was classified as very strong inhibition. Conclusion: Persea Americana Mill. seed extract had a very strong inhibitory effect on the growth of Streptococcus mutans.Keywords: avocado seed (Persea americana Mill.), Streptococcus mutans, inhibition effectAbstrak: Selain menjadi bahan konsumsi masyarakat yang lezat, ternyata alpukat telah lama dipercaya dapat mengobati penyakit di dalam rongga mulut. Di dalam buah alpukat terdapat biji yang terbukti melalui penelitian ilmiah mengandung flavonoid, alkaloid, dan tannin yang mampu menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji buah alpukat (Persea americana Mill.) terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan ialah modifikasi Kirby-Bauer dengan paper disk. Kontrol positif menggunakan antibiotik eritromisin dan kontrol negatif menggunakan akuades. Biji buah alpukat diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi Manado. Hasil penelitian menunjukkan diameter rerata zona hambat dari ekstrak biji buah alpukat yang terbentuk ialah 21,8 mm dan digolongkan sebagai zona hambat sangat kuat. Simpulan: Ekstrak biji buah alpukat memiliki daya hambat sangat kuat terhadap pertumbuhan Streptococcus mutans.Kata kunci: biji buah alpukat (Persea americana Mill.), Streptococcus mutans, daya hambat

scholarly journals Uji konsentrasi hambat minimum (KHM) ekstrak daun pacar air (Impatiens balsamina L.) terhadap pertumbuhan Streptococcus mutans

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Raymond A. Lolongan ◽  
Olivia Waworuntu ◽  
Christy N. Mintjelungan
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Microbiology Laboratory ◽  
Control Group Design ◽  
Group Design ◽  
Impatiens Balsamina ◽  
Herbal Plant

Abstract: Impatiens balsamina L. is an herbal plant which contains flavonoids that has been proven to inhibit the growth of Streptococcus mutans. This study was aimed to determine the minimum inhibitory concentration (MIC) of the balsamina leaf extract on the growth of Streptococcus mutans. This was a true experimental study with a randomized pretest-postest control group design. The method used was serial dilution and tested with the spectrophotometer and turbiditry measurements. Balsamina leaf was extracted by using maceration method with 96% ethanol. The Streptococcus mutans bacteria were obtained from pure stock bacteria at Microbiology Laboratory of Pharmacy Faculty of Mathematics, University of Sam Ratulangi. The results indicated that the minimum inhibitory concentration (MIC) of the balsamina leaf (Impatiens balsamina L.) extract on the growth of Streptococcus mutans was at a concentration of 3.125%.Keywords: balsamina leaf (Impatiens balsamina L.), Streptococcus mutans, MIC Abstrak: Pacar Air (Impatiens balsamina L.) merupakan tanaman herbal yang mengandung flavonoid yang telah terbukti dapat menghambat pertumbuhan Streptococcus mutans. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) dari ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans. Jenis penelitian ialah eksperimental murni dengan Randomized Pretest-Posttest Control Group Design. Metode yang digunakan dalam penelitian ini yaitu metode serial dilusi dengan metode pengujian turbidimetri dan spektrofotometri. Daun Pacar air diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Streptococcus mutans diperoleh dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian ini menunjukkan bahwa konsentrasi hambat minimum (KHM) ekstrak daun Pacar Air terhadap pertumbuhan Streptococcus mutans pada konsentrasi 3,125%. Kata kunci: daun pacar air (Impatiens balsamina L), Streptococcus mutans, KHM

scholarly journals The effect of papaya leaf extract (Carica papaya l.) to the bleeding time on mice with trombositopenia

2018 ◽  
Vol 6 (3) ◽  
pp. 133
Author(s):  
Saktya Yudha Ardhi Utama
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Bleeding Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Mean Time

<p align="center"><strong>ABSTRAK</strong></p><p><strong>Latar belakang: </strong>Trombositopenia merupakan suatu keadaan jumlah trombosit di bawah 150.000/mm<sup>3</sup>, hal ini dapat menyebabkan perdarahan yang apabila tidak ditangani dengan baik dapat berujung pada kematian. Beberapa tindakan yang dapat dilakukan untuk mengatasi  trombositopenia, salah satunya adalah dengan tanaman daun pepaya.</p><p><strong>Tujuan:</strong> untuk menganalisis pengaruh pemberian ekstrak daun pepaya terhadap <em>bleeding time</em> (waktu perdarahan).</p><p><strong>Metode : </strong>Desain penelitian ini menggunakan <em>true experimental</em> dengan rancangan <em>post test only control group design</em>. Sampel dalam penelitian ini sejumlah 30 ekor mencit jantan (<em>Mus musculus</em>) yang dibagi menjadi lima kelompok. Kelompok pertama sebagai kelompok kontrol negatif (CMC Na 0,5%), kelompok kedua sebagai kontrol positif (CMC Na 0,5%)<a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_1">[a1]</a> <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_2">[A2]</a> , kelompok ketiga diberi esktrak etanol 96% daun pepaya dosis 0,5g/kgBB, kelompok keempat diberi esktrak etanol 96% daun pepaya dosis 1g/kgBB, kelompok kelima diberi esktrak etanol 96% daun pepaya dosis 2g/kgBB. Pengumpulan data menggunakan lembar pengukuran <em>bleeding time</em>, yang dianalisa dengan uji ANOVA.</p><p><strong>Hasil: </strong>Hasil penelitian ini menunjukkan bahwa didapatkan nilai <em>p</em>&lt;0.0001 dengan rerata waktu paling cepat antara kelompok perlakuan yaitu kelompok perlakuan dengan dosis 2g/kgBB (2.74±0.14). Hal ini menunjukkan bahwa terdapat perbedaan signifikan antar semua kelompok. <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_3">[a3]</a> <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_4">[A4]</a> </p><p><strong>Kesimpulan: </strong>Kesimpulan dari penelitian ini adalah pemberian ekstrak daun pepaya (<em>Carica papaya L.</em>) dapat mempercepat <em>bleeding time</em> (waktu perdarahan).</p><p><strong>KATA KUNCI: </strong>daun pepaya; <em>bleeding time, </em>trombositopenia<strong></strong></p><p align="center"> </p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background</em></strong><strong>: </strong>Thrombocytopenia is a condition of platelet counts below 150,000/mm<sup>3</sup>, this may cause bleeding which, if not handled properly, may lead to death. Some actions that can be done to overcome thrombocytopenia, one of which is with papaya leaf.</p><p><strong>Objectives:</strong> This study aims to analyze the effect of giving papaya leaf extract to bleeding time.</p><p><strong><em>Methods:</em></strong><strong> </strong>This research design uses true experimental with design of post-test only control group design. The sample in this study were 30 male mice (<em>Mus musculus</em>) divided into five groups. The first group as the negative control group (CMC Na 0.5%), the second group as the positive control (CMC Na 0.5%), the third group was given ethanol 96% extract papaya leaf dose 0.5g/kgBW, the fourth group was given ethanol 96% extract papaya leaf dose 1g/kgBW, the fifth group was given ethanol 96% extract papaya leaf dose 2g/kgBW. Data collection using measurement sheet of  bleeding time which were analyzed by ANOVA test.</p><p><strong><em>Results:</em></strong><strong> </strong>The results of this study showed that p&lt;0.0001 the fastest mean time occur between treatment groups that is treatment group with dose 2g/kgBW (2.74±0.14). In the bleeding time variables p&lt;0.0001 the fastest mean time occur between treatment groups that is treatment group with dose 2g/kgBW (2.74±0.14). This result shows that there are significant differences between every groups.</p><p><strong><em>Conclusion:</em></strong><strong> </strong>The conclusion of this research is giving papaya leaf extract can increase bleeding time.</p><p> </p><p><strong>KEYWORDS: </strong>leaf of papaya, bleeding time, thrombocytopenia.</p><p> </p>

scholarly journals PERAN EKSTRAK DAUN WUNGU (GRAPTOPHYLLUM PICTUM L. GRIFF) TERHADAP ADHESI STREPTOCOCCUS MUTANS PADA NEUTROFIL

2020 ◽  
Vol 11 (2) ◽  
pp. 128-134
Author(s):  
Atik Kurniawati ◽  
Sulistiyani ◽  
Arina Nur Rahmah
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Streptococcus mutans (S. mutans) merupakan bakteri utama penyebab karies gigi yang mampu mendemineralisasi email, menginvasi dentin yang dapat berlanjut menjadi inflamasi pulpa (pulpitis). Streptococcus mutans menginvasi inang diawali dengan melakukan adhesi (perlekatan). Proses ini merupakan langkah awal dalam proses inflamasi. Oleh karena itu, adhesi Streptococcus. mutans pada sel inang perlu dicegah agar tidak terjadi infeksi oleh bakteri. Neutrofil merupakan sel pertahanan pertama yang datang pada proses inflamasi. Salah satu upaya untuk mencegah terjadinya adhesi tersebut bisa menggunakan bahan alami yang bersifat anti-inflamasi, salah satunya yaitu daun wungu. Penelitian ini bertujuan untuk  mengetahui pengaruh pemberian ekstrak daun wungu (EDW) terhadap adhesi  bakteri  Streptococcus. mutans pada neutrofil dan perbedaan indeks adhesi dalam berbagai konsentrasi dengan eksperimental laboratoris menggunakan the post test only control group design. Sampel terbagi menjadi 5 kelompok (klp): klp I/kontrol (tanpa inkubasi EDW), klp II (EDW 3,125%), klp III (EDW 6,25%), klp IV (EDW 12,5%), dan klp V (EDW 25%). Isolat neutrofil diinkubasi dengan EDW selama 3 jam, kemudian dipapar S. mutans selama 4 jam. Indeks adhesi dihitung berapa rata-rata jumlah S. mutans  yang menempel pada 100 neutrofil. Simpulan penelitian menunjukkan bahwa ekstrak etanol daun wungu berpotensi menurunkan indeks adhesi Streptococcus mutans. Kata kunci: adhesi, S. mutans, neutrophil, daun wungu

scholarly journals EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH MERAH (Piper Crocatum) DAN EKSTRAK BIJI ALPUKAT (Persea americana) DALAM MENGHAMBAT PERTUMBUHAN Candida albicans

2017 ◽  
Vol 2 (2) ◽  
pp. 86 ◽  
Author(s):  
Vini Anggraini ◽  
Masfufatun Masfufatun
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Betel Leaf ◽  
Post Test ◽  
Avocado Seed

Abstrak   Candida albicans merupakan salah satu mikrooganisme penyebab infeksi kandidiasis. Daun sirih merah (Piper crocatum) dan biji alpukat (Persea americana) telah diteliti dapat menghambat pertumbuhan Candida albicans. Tujuan penelitian ini adalah untuk mengetahui efektivitas kombinasi daun sirih merah (Piper crocatum) dan ekstrak biji alpukat (Persea americana) dalam menghambat pertumbuhan Candida albicans. Penelitian bersifat eksperimental laboratorium (true experiment) dengan pendekatan post test control group design only. Daun sirih merah dan biji alpukat diekstraksi secara maserasi menggunakan pelarut etanol.    Pada masing-masing ekstrak etanol dilakukan uji pendahuluan untuk mengetahui konsentrasi optimum ektrak dalam memnghambat pertumbuhan C. albicans. Daya Hambat pertumbuhan C. albicans diuji menggunakan metode difusi. Hasil penelitian menunjukkan bahwa ekstraks daun sirih merah dan biji alpukat masing-masing memiliki daya hambat terbesar pada konsentrasi 10%. Kombinasi ekstrak daun sirih merah dan ekstrak biji alpukat memiliki daya hambat yang signifikan terhadap pertumbuhan Candida albicans dibandingakan kontrol negatif, kontrol positif dan ekstrak daun sirih merah. Dengan demikian kombinasi ekstrak daun sirih dan biji aplukat etanol berpotensi sebagai antifungi dalam menghambat pertumbuhan C. albicans sehingga diharapkan bisa mengurangi prevalensi kandidiasis. Kata Kunci :    daun sirih merah,  biji alpukat, Candida albicans,                                                           Abstract Candida albicans is one of the microbialism that causes candidiasis infection. Red betel leaf (Piper crocatum) and avocado seed (Persea americana) have been studied to inhibit the growth of C. albicans. The purpose of this study was to determine the effectiveness of red betel leaf (Piper crocatum) and avocado seed extract (Persea americana) combination in inhibiting the growth of C. albicans. Research is experimental laboratory (true experiment) with post test approach control group design only. Red betel leaf and avocado seeds are extracted by maceration using ethanol solvent. In each ethanol extract, a preliminary test was performed to find out the optimum concentration of extract in inhibiting the growth of C. albicans. C. albicans growth retardant was tested using diffusion method. The results showed that the extract of red betel leaf and avocado seeds each had the greatest inhibitory concentration at 10%. The combination of red betel leaf extract and avocado seed extract have significant inhibitory effect on C. albicans growth compared to negative control, positive control and red betel leaf extract. Thus, the combination of betel leaf extract and ethanol  seed has the potential as an antifungal in inhibiting the growth of C. albicans so it is expected to reduce the prevalence of candidiasis. Keywords: red betel leaf, avocado seed, Candida albicans, 

scholarly journals Uji Daya Hambat Ekstrak Tinta Cumi-cumi (Loligo sp) terhadap Pertumbuhan Bakteri Streptococcus mutans

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Rocky J. Mangindaan ◽  
Christy N. Mintjelungan ◽  
Damajanty H. C. Pangemanan
Keyword(s):  
Streptococcus Mutans ◽  
Inhibitory Effect ◽  
Control Group ◽  
Strong Inhibition ◽  
Control Group Design ◽  
Group Design ◽  
Mean Diameter ◽  
Post Test ◽  
Strong Inhibitory Effect ◽  
The Mean

Abstract: Dental caries is still a health problem in Indonesia. There are several factors that play some important roles in the occurence of caries, as follows: microorganism, host, food, and time. Streptococcus mutans is one of the microorganisms that cause caries. Squid ink contains melanin which has an active compound to inhibit microbial activity. This study was aimed to determine the inhibitory effect of squid ink extract (Loligo sp) on the growth of Streptococcus mutans. This was a laboratory experimental study, with a post test only control group design. The results showed that the mean diameter of the inhibitory zones of the squid ink extract (Loligo sp) was 10.50 mm which was categorized as strong inhibition (Davis and Stout criteria). In conclusion, the squid ink extract (Loligo sp) had a strong inhibitory effect on the growth of Streptococcus mutans bacteria.Keywords: squid ink extract (Loligo sp), Streptococcus mutans, zone of inhibitionAbstrak: Karies gigi merupakan salah satu masalah kesehatan gigi dan mulut di Indonesia. Faktor-faktor penyebab karies gigi yaitu mikroba, pejamu, makanan, dan waktu. Salah satu mikroba penyebab karies ialah bakteri Streptococcus mutans. Tinta cumi-cumi mengandung melanin yang memiliki senyawa aktif untuk menghambat aktivitas mikroba. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri Streptococcus mutans. Jenis penelitian ialah eksperimental laboratorik, dengan post test only control group design. Hasil penelitian menunjukkan diameter rerata zona hambat dari ekstrak tinta cumi-cumi (Loligo sp) sebesar 10,50 mm dan digolongkan dalam kategori kuat (kriteria Davis dan Stout). Simpulan penelitian ini ialah ekstrak tinta cumi-cumi (Loligo sp) memiliki daya hambat yang kuat terhadap pertumbuhan bakteri Streptococcus mutans.Kata kunci: ekstrak tinta cumi-cumi (Loligo Sp), Streptococcus mutans, zona hambat

scholarly journals Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Oktovianus Pormes ◽  
Damajanty H.C. Pangemanan ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Amaranthus Hybridus ◽  
Group Design ◽  
Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

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