scholarly journals Perbandingan Aktivitas Antioksidan Ekstrak Etanol dan Heksana dari Daun Gedi Merah (Abelmoschus manihot)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Juan A. W. Prawira
Keyword(s):  
Antioxidant Activity ◽  
Lipid Oxidation ◽  
Total Phenolic Content ◽  
Oxidation Process ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Inhibition Activity ◽  
Leaf Powder

Penelitian ini bertujuan untuk menentukan perbandingan aktivitas antioksidan ekstrak etanol dan heksana daun gedi merah dalam menghambat proses oksidasi lipida. Penelitian dimulai dengan mengekstraksi serbuk daun gedi merah yang sudah dikeringanginkan menggunakan cara maserasi selama 48 jam dengan pelarut etanol 75% dan pelarut heksana, selanjutnya ditentukan kandungan total fenolik serta aktivitas penghambatan oksidasi. Aktivitas penghambatan oksidasi diukur berdasarkan analisis kadar peroksida dan malondialdehida (MDA). Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki kandungan total fenolik sebesar 28,68 mg/kg dan ekstrak n-heksana sebesar 2,86 mg/kg. Kemampuan penghambatan peroksida maupun MDA oleh ekstrak etanol lebih tinggi daripada ekstrak n-heksana.The purpose of this research is to compare the antioxidant activity of ethanol and hexane extract of leaves of red gedi in inhibiting lipid oxidation process. The research begins by extracting the red gedi leaf powder that has been dried using maceration for 48 hours with 75% ethanol and hexane solvent, then determined the total phenolic content and oxidation inhibition activity. Oxidation inhibition activity was measured by analysis of the levels of peroxide and malondialdehyde (MDA). The results showed that the ethanol extract has a total phenolic content of 28.68 mg/kg and n-hexane extract at 2.86 mg/kg. The ability MDA and peroxide inhibition by ethanol extract higher than n-hexane extract.

scholarly journals CONTRIBUTION TO THE PHYTOCHEMICAL STUDY AND CHEMICAL TESTS OF THE EXTRACTS OF Citrus limonium (LEMON) and Capsicum frutescens L. (CHILLI PEPPER)

2015 ◽  
Vol 1 ◽  
pp. 21
Author(s):  
Laiza Cristina Carlos Freire ◽  
Emanuel Isaias Mota Araújo ◽  
Francisca Ítala da Silva Paiva Valdevino ◽  
Francisco Felipe Maia da Silva ◽  
Leonardo Alcântara Alves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Capsicum Frutescens ◽  
Chilli Pepper ◽  
Phytochemical Study ◽  
Natural Medicine

Use of natural medicine has been used by man since time immemorial, and this use has grown considerably in recent times. Thus, this study aims to make a comparator analysis regarding the phytochemical test, antioxidant activity and total phenolic content of the extracts of the leaves in ethanol and their respective fractions of Citrus limonium (lemon) and Capsicum frutescens L. (chilli pepper). To compare them was observed that ethanol extract of both plants showed the better antioxidant activity, Capsicum frutescens L. with 204.60 ppm and 99.07 ppm with Citrus limonium. With regard to the phenolic content of the hexane extract and lemon extract in ethanol pepper were those that showed a higher content. Overall, it was noted that Citrus limonium excelled both in relation to their antioxidant activity and the phenolic content.  

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN n-HEKSANA DARI DAUN RUMPUT SANTA MARIA (Artemisia vulgaris L.) PADA MINYAK IKAN

2014 ◽  
Vol 14 (2) ◽  
pp. 129
Author(s):  
Erpi Bangol ◽  
Lidya I. Momuat ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Fish Oil ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Artemisia Vulgaris ◽  
Leaves Of Grass ◽  
Hexane Extracts ◽  
Santa Maria

ABSTRAK Penelitian ini bertujuan untuk menentukan kandungan total fenolik dan aktivitas antioksidan ekstrak etanol dan n-heksana dari daun Artemisia vulgaris L. Total fenolik diukur menggunakan metode Follin-Ciocalteau dengan asam galat sebagai larutan standar. Aktivitas antioksidan diukur berdasarkan analisis kadar peroksida dan malonaldehida (MDA) dari minyak ikan. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki kandungan total fenolik sebesar 81.22 mg/kg dan ekstrak n-heksana sebesar 20.10 mg/kg. Kemampuan penghambatan peroksida maupun MDA oleh ekstrak etanol lebih tinggi daripada ekstrak n-heksana. Sebagai pembanding, α-tokoferol memiliki aktivitas penghambatan peroksida dan MDA lebih tinggi daripada ekstrak n-heksana, tetapi lebih rendah daripada ekstrak etanol. Kata kunci :Artemisia vulgaris L., antioksidan, fenolik, peroksida, malonaldehida   ANTIOXIDANT ACTIVITY OF THE ETHANOL AND n-HEXANE EXTRACTS OF LEAVES OF GRASS SANTA MARIA (Artemisia vulgaris L.) IN FISH OIL ABSTRACT The purpose of the study was to determine the total phenolic content and antioxidant activity of the ethanol and n-hexane extracts of leaves of Artemisia vulgaris L. Total phenolic was determined using Follin-Ciocalteau method with gallic acid as a standard solution. The antioxidant activity was measured based on the analysis of the levels of peroxide and malonaldehida (MDA) in fish oil. The results showed that the ethanol extract had a total phenolic content of 81.224 mg/kg and n-hexane extracts of 20.101 mg/kg. The ability of peroxide and MDA inhibition by ethanol extract was higher than that by n-hexane extract. For comparison, α-tocopherol has higher inhibitory activity on peroxide and MDA than n-hexane extracts, but lower than ethanol extract. Keywords: Artemisia vulgaris L., antioxidants, phenolic, peroxide, malonaldehida

scholarly journals Aktivitas Antioksidan Ekstrak Fenol Daun Gayam (Inocarpus fagiferus Fosb)

Biosfera ◽  
2018 ◽  
Vol 35 (1) ◽  
pp. 37
Author(s):  
Dwi Marga Lestari ◽  
Nurul Mahmudati ◽  
Sukarsono Sukarsono ◽  
Nurwidodo Nurwidodo ◽  
Husamah Husamah
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Assay Method ◽  
Total Phenolic ◽  
Powder Samples ◽  
Leaf Powder ◽  
Dark Green

This study aims to determine the total phenolic content and antioxidant activity in gayam leaf extract (Incarpus fagiferus Fobs). The research method used is a quasi-experiment that aims to predict the situation to be achieved through actual experiments but no treatment. The sample used is old gayam leaves, with the characteristic of dark green leaf and rough leaf surface. The process of preparing simplicia, ie preparing fresh gayam leaves, dried in an oven temperature 45-50oC, and then dried to produce gayam leaf powder. Samples were extracted with methanol solvent and ethanol for 5 days. The total phenol assay method uses Folin-Ciocalteau method and antioxidant activity test using DPPH free radical retardation method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the total phenolic content of gayam leaf extract with ethanol and methanol solvent was 313,704 GAE (Gallic Acid Equivalent) and 273,913 GAE, respectively. Antioxidant activity as a free antidote to free radical DPPH is known to be valued with IC50 (inhibitory concentration).

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE

Biomedika ◽  
2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Ika Trisharyanti Dian Kusumowati ◽  
Rosita Melannisa ◽  
Kartikaning Ratri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Positive Correlation

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds

scholarly journals The antioxidant properties and α-amylase inhibition activities of polyphyto mixture with honey formulations

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2190-2196
Author(s):  
W.N.A.S.W.M. Rashidi ◽  
N. Muhammad ◽  
N. Abdullah ◽  
B.A. Talip ◽  
N. Bahrin
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
Interaction Effect ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Inhibition Activity ◽  
Dpph Assay ◽  
Ficus Deltoidea

Ficus carica, Orthosiphon stamineus, Ficus deltoidea and Trigona honey are known for their high antioxidant amount. In this research, these plants and honey were used to study their interactions in terms of the antioxidant properties and the inhibition of α-amylase enzyme. These plants samples were chosen as they are usually used in traditional medicine to treat diabetes. Therefore, the objectives of this study are to measure the antioxidant activity as well as the α-amylase enzyme inhibition properties of the polyphyto formulations and Trigona honey. Simplex Centroid Mixture Design (SCMD) was used to design the formulations. In this experiment, the highest (p<0.05) antioxidant content for 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was a formulation of 100% Trigona honey (90.09%). Meanwhile, a mixture of 50% F. carica – 50% O. stamineus and 50% O. stamineus – 50% Trigona honey yield the highest (p<0.05) total phenolic content (TPC) value (133.77 and 133.17 μg/mL, respectively), which indicated a synergistic interaction effect. However, for the measurement of ferric reduction antioxidant potential (FRAP), the formulation of 100% O. stamineus and 100% F. deltoidea exhibits the highest (p<0.05) value (148.95 and 148.78 μg/mL, respectively). In terms of enzyme inhibition, F. deltoidea showed the highest inhibition activity for α-amylase (8.826%). In conclusion, different percentages of F. carica, O. stamineus, F. deltoidea and Trigona honey have different interaction effects on the antioxidant activity and α-amylase inhibition depending on the amount of each factor.

scholarly journals Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia

2019 ◽  
Vol 20 (2) ◽  
pp. 576-580
Author(s):  
HENNY NURHASNAWATI ◽  
REKSI SUNDU ◽  
SAPRI SAPRI ◽  
RISA SUPRININGRUM ◽  
HARLINDA KUSPRADINI ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Indigenous Species ◽  
Flavonoid Content ◽  
East Kalimantan ◽  
Phenolic And Flavonoid

Abstract. Nurhasnawati H, Sundu R, Sapri, Supriningrum R, Kuspradini H, Arung ET. 2019. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas 20: 576-580. This study aimed to determine the total phenolic and flavonoids content and antioxidant activity of ethanol extract of several indigenous species of ferns in East Kalimantan. Total phenolic content was determined by Folin-Ciocalteau method and flavonoid content was measured by colorimetric method. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The result of photochemical screening indicated that the leaves of Plagiogyria pycnophylla, Plagiogyria glauca, and Stenochlaena palustris contained alkaloid, flavonoid, tannin, saponin, and steroid while Acrostichum aureum contained flavonoid, tannin, saponin and steroid. The result showed extract Acrostichum aureum had the highest total phenolic content (366.4573 ± 2.2117 mg GAE.g-1), flavonoid content (228.6087 ± 2.2548 mg QE.g-1), and very strong antioxidant activity with IC50 value 29.5303 ppm. There is positive correlation between total phenolic content, flavonoid with antioxidant activity.

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

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