scholarly journals PEMISAHAN JENIS PIGMEN KAROTENOID DARI KEPITING Grapsus sp JANTAN MENGGUNAKAN METODE KROMATOGRAFI KOLOM

2019 ◽  
Vol 7 (2) ◽  
pp. 121
Author(s):  
Anisa ET Silaa ◽  
Darus SJ Paransa ◽  
Anton P Rumengan ◽  
Kurniati Kemer ◽  
Natalie DC Rumampuk ◽  
...  
Keyword(s):  
Column Chromatography ◽  
High Performance ◽  
Black Body ◽  
The Body ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Chromatography Method ◽  
Stone Crab ◽  
Yellow Orange ◽  
Orange Color

Grapsus sp crab has a greenish black body color and also known as stone crab. Crabs in the genus Grapsus sp have a swift movement, long legs, they do not have swimming legs and have small reddish purple or purple-orange color, claws on the body of this crab indicated the presence of pigments such as carotenoid pigments. Carotenoid pigments are one form of secondary metabolites which consist of carotene and xanthophyll groups. Carotenoid pigments are present in yellow, orange or orange red which are also found in crabs. Separation of carotenoid pigments can be done using the TLC method, High Performance Liquid Chromatography (HPLC) and Column Chromatography (CC). The purpose of this study was to determine the type of carotenoid pigment from male Grapsus sp crab extract using the Column Chromatography separation method. From the results of this study, the carotenoid pigment content in the 1,2 and 3 carapace was 46,85 μg, 39 µg, and 33,14 µg. The carotenoid pigment concentrations in carapace extracts 1,2 and 3 are 25,38 µg/g, 23,4 µg/g and 5,11 µg/g. From the results of the separation using the column chromatography method, the type of carotenoid pigment identified from the carapace extract of Grapsus sp male is β-Carotene, Ekinenon, Astaxantine, Kantaxantine and Astacen.Keywords: Grapsus sp, Carotenoid, Column ChromatographyKepiting Grapsus sp memiliki warna tubuh hitam kehijauan dan dikenal dengan nama kepiting batu. Kepiting dalam genus Grapsus sp memiliki gerakkan yang cekatan, mempunyai kaki yang panjang, tidak memiliki kaki renang dan memiliki capit berukuran kecil yang berwarna ungu kemerahan atau ungu-oranye warna pada tubuh kepiting ini mengindikasikan adanya kandungan pigmen seperti pigmen karotenoid. Pigmen karotenoid merupakan salah satu bentuk metabolit sekunder yang yang terdiri dari golongan karoten dan xantofil. Pigmen karotenoid  hadir dalam warna kuning, oranye, atau merah oranye, yang juga ditemukan pada kepiting. Pemisahan pigmen karotenoid dapat dilakukan dengan menggunakan metode KLT, Kromatografi Cair Kinerja Tinggi (KCKT) dan Kromatografi Kolom (KK). Tujuan dari penelitian ini adalah untuk mengetahui jenis pigmen karotenoid dari ekstrak kepiting Grapsus sp Jantan dengan menggunakan metode pemisahan Kromatografi Kolom. Dari hasil penelitian ini, didapatkan kandungan pigmen karotenoid pada karapas 1,2 dan 3 adalah 46,85 µg 39 µg, dan 33,14 µg. Konsentrasi pigmen karotenoid pada ekstrak karapas 1,2 dan 3 adalah 25,38 µg/g, 23,4 µg/g dan 5,11 µg/g. Hasil pemisahan menggunakan metode pemisahan kromatografi kolom didapatkan ekstrak karapas kepiting Grapsus sp jantan memiliki jenis pigmen β-Karoten, Ekinenon, Astaxantin, Kantaxantin dan Astasen.Kata kunci: Grapsus sp, Karotenoid, Kromatografi Kolom, 

scholarly journals Analysis of Carotenoid Pigment Types in the Carapace of the Male Crab Grapsus albolinetaus Latreille in Milbert 1812

2021 ◽  
Vol 9 (1) ◽  
pp. 132
Author(s):  
Fatika Sari Mokoginta ◽  
Darus Saadah Johanis Paransa ◽  
Kurnati Kemer ◽  
James J. H. Paulus ◽  
Nickson J. Kawung ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Column Chromatography ◽  
Metabolic Pathways ◽  
Mobile Phase ◽  
Separation Technique ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Silica Powder ◽  
Male Crab ◽  
Yellow Orange

Carotenoid pigments have various colors such as yellow, orange, or red-orange. One of the carotenoid pigment sources is Crab G. albolineatus Latreille in Milbert 1812. Column chromatography separation technique was used to determine the metabolism of carotenoid pigments in the crab G. albolineatus latreille in Milbert 1812. This CC separation used hexane and acetone as the developer solution (70:30). The developer solution as known as the mobile phase is semipolar, while the stationary phase is silica powder G60. Therefore, it formed two metabolic pathways. The male G. albolineatus crab used in this study was on the D3 molting stage which had a concentration of 36.37 g/g dry residue and 4.72 g content. The types of pigments identified are: β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Keywords: Carotenoid Pigments; G. albolineatus; Column Chromatography; MoltingAbstrakPigmen karotenoid memiliki berbagai warna seperti kuning, oranye, atau merah oranye. Salah satu sumber pigmen karotenoid adalah pada karapas kepiting G. albolineatus Latreille in Milbert 1812. Untuk mengetahui metabolisme jenis pigmen karotenoid pada kepiting Grapsus albolineatus latreille in Milbert 1812 yaitu menggunakan pemisahan kromatografi Kolom. Pemisahan KK ini menggunakan larutan pengembang heksan dan aseton (70:30). Larutan pengembang merupakan fase gerak yang bersifat semipolar dan fase diamnya menggunakan bubuk silika G60. Terbentuk dua  jalur metabolisme. Kepiting G. albolineatus jantan yang digunakan pada penelitian berada di stadium molting D3 dengan konsentrasi sebesar 36,37 µg/g berat residu kering dan kandungan 4,72 µg. Jenis pigmen yang teridentifikasi yaitu : β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Kata kunci: Pigmen; Karotenoid; G. albolineatus; Kromatofrafi Kolom; Molting

scholarly journals Analysis Of Types Of Carotenoid Pigments In Crab Sesarmops sp From Manado By Coast

2021 ◽  
Vol 9 (2) ◽  
pp. 204
Author(s):  
Melinda Margareta Adrian ◽  
Darus S. J Paransa ◽  
James J. H Paulus ◽  
Nickson J. Kawung ◽  
Robert A. Bara ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Chromatographic Methods ◽  
Total Pigment ◽  
Male Crab ◽  
Yellow Orange ◽  
Pigment Extract ◽  
Mangrove Crabs ◽  
Layer Chromatography

Carotenoid pigments are a group of pigments that are yellow, orange, and red-orange in color. Pigments are natural dyes found in plants and animals, extracts of carotenoid pigments can be separated by chromatographic methods where the common chromatographic methods in determining the type of pigment are Column Chromatography (CC) and Thin Layer Chromatography (TLC). The purpose of this study was to determine the types of pigments contained in the carapace extract of the male crab Sesarmops sp. The Sesarmops sp crab has a brown dorsal carapace with blackish-brown leg spots known as mangrove crabs. Crabs in the Sesarmidae family have a carapace formation and wide legs, do not have swimming legs, and have a pair of claws that are faded purple, the presence of these colors can be identified as containing carotenoid pigments. The results of this study obtained the content of carotenoid pigment 25.2 g/gr and the value of the concentration of carotenoid pigment in the male crab extract Sesarmops sp 10.99 g. The results of the separation of the total pigment extract using column chromatography obtained the types of pigments -carotene, Ekinenon, Zeaxanthin, and Astaxanthin. Keywords: Column Chromatography (CC); Carotenoids; Sesarmops sp                                                                       AbstrakPigmen karotenoid adalah sekelompok pigmen yang berwarna kuning, oranye dan merah oranye. Pigmen adalah zat warna alami yang terdapat pada tumbuhan  dan hewan, ekstrak pigmen karotenoid dapat dipisahkan dengan metode kromatografi yang dimana metode kromatografi umum dalam menentukan jenis pigmen adalah Kromatografi Kolom (KK) dan Kromatografi Lapis Tipis (KLT). Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen apa saja yang terdapar pada ekstrak karapas kepiting jantan Sesarmops sp. Kepiting Sesarmops sp memiliki karapas dorsal bewarna coklat dengan bintik kaki coklat kehitaman yang dikenal sebagai kepiting mangrove. Kepiting dalam keluarga sesarmidae memiliki bentukan karapas dan kaki yang lebar tidak memiliki kaki renang serta memiliki sepasang capit berwarna ungu pudar, adanya warna tersebut kepiting dapat diidentikasikan mengandung jenis pigmen karotenoid.  Dari hasil penelitian ini mendapatkan kandungan pigmen karotenoid 25,2 dan nilai konsentrasi pigmen karotenoid pada ekstrak kepiting jantan Sesarmops sp 10,99 . Hasil pemisahan dari ekstrak pigmen total menggunakan kromatografi kolom didapatkan jenis pigmen β-karoten, Ekinenon, Zeaxantin dan Astaxantin

scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

scholarly journals The Identification of Carotenoids and Testing of Carotenoid Antioxidants from Sand Lobster (Panulirus homarus) Egg Extract

2017 ◽  
Vol 22 (3) ◽  
pp. 155
Author(s):  
James Ngginak ◽  
Jubhar C Mangibulude ◽  
Ferdy S Rondonuwu
Keyword(s):  
Free Radicals ◽  
High Performance ◽  
Carotenoid Content ◽  
Test Results ◽  
Antioxidant Power ◽  
Sample Extract ◽  
Egg Extract ◽  
Dpph Method ◽  
Yellow Orange ◽  
Orange Color

Sand lobsters (Panulirus homarus) are organisms that play an important role in supporting a society’s economy. Lobsters have a business appeal due to their high demand, enjoyable taste, as well as significant nutritional content. It is interesting that sand lobster eggs have a yellow orange color as an indicator of the presence of carotenoid content. Related with advancements in research and the utilization of carotenoids, this research identifies carotenoids and tests carotenoid antioxidants from sand lobster (Panulirus homarus) egg extract. In identifying carotenoids in sand lobster egg extract, HPLC (high performance liquid chromatography) (shimadzu LC-10AD, Japan) is used. In testing carotenoid antioxidants from sand lobster eggs, a DPPH method is utilized. The identification results with HPLC reveal that the sample contains carotenoids. The kinds of carotenoids identified are dinoxanthin, diadinoxanthin, zeaxanthin, lutein, astaxanthin, and violaxanthin. Among the six kinds of carotenoids identified, lutein is the type of carotenoid that has the highest concentration. These carotenoid compounds are detected in wavelengths of 400-500 nm. The test results of antioxidant power reveal that to hamper free radicals (IC50), a sample concentration of 6675.25 µg.ml-1 is needed. The ability to hinder free radicals from a sample extract is mostly conducted by lutein, zeaxanthin, and astaxantin. If seen from the analytical results, it can be surmised that sand lobster eggs contain carotenoids that can be used as a carotenoid source for humans.   Keywords : Sand Lobster, HPLC, Carotenoids, DPPH, Antioxidant

scholarly journals A simple, rapid and validated high-performance liquid chromatography method suitable for clinical measurements of human mercaptalbumin and non-mercaptalbumin

2017 ◽  
Vol 55 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Keiko Yasukawa ◽  
Tatsuo Shimosawa ◽  
Shigeo Okubo ◽  
Yutaka Yatomi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Practical Method ◽  
The Body ◽  
Serum Samples ◽  
Chromatography Method ◽  
Anion Exchange Column ◽  
Liquid Chromatography Method ◽  
Analytical Time

Background Human mercaptalbumin and human non-mercaptalbumin have been reported as markers for various pathological conditions, such as kidney and liver diseases. These markers play important roles in redox regulations throughout the body. Despite the recognition of these markers in various pathophysiologic conditions, the measurements of human mercaptalbumin and non-mercaptalbumin have not been popular because of the technical complexity and long measurement time of conventional methods. Methods Based on previous reports, we explored the optimal analytical conditions for a high-performance liquid chromatography method using an anion-exchange column packed with a hydrophilic polyvinyl alcohol gel. The method was then validated using performance tests as well as measurements of various patients’ serum samples. Results We successfully established a reliable high-performance liquid chromatography method with an analytical time of only 12 min per test. The repeatability (within-day variability) and reproducibility (day-to-day variability) were 0.30% and 0.27% (CV), respectively. A very good correlation was obtained with the results of the conventional method. Conclusions A practical method for the clinical measurement of human mercaptalbumin and non-mercaptalbumin was established. This high-performance liquid chromatography method is expected to be a powerful tool enabling the expansion of clinical usefulness and ensuring the elucidation of the roles of albumin in redox reactions throughout the human body.

scholarly journals Amelioration on oxidative stress, testosterone, and cortisol levels after administration of Vitamins C and E in albino rats with chronic variable stress

2021 ◽  
Vol 14 (1) ◽  
pp. 137-143
Author(s):  
Nanik Hidayatik ◽  
Agus Purnomo ◽  
Faisal Fikri ◽  
Muhammad Thohawi Elziyad Purnama
Keyword(s):  
High Performance ◽  
The Body ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Albino Rats ◽  
Serum Samples ◽  
Chromatography Method ◽  
Body Weight Data ◽  
Chronic Variable Stress ◽  
Liquid Chromatography Method

Background and Aim: Stress can cause physiological and biological disorders in the body. On the other hand, antioxidants from vitamins and minerals are effective for stress treatment. Therefore, this study aimed to evaluate the effect of the administration of Vitamins C and E on serum superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx), testosterone, and cortisol activity in albino rats with chronic variable stress (CVS). Materials and Methods: Twenty albino rats were randomly assigned into four treatment groups: C was administered normal saline; T1 was administered Vitamins C and E; T2 was only induced CVS; and T3 was induced CVS followed by Vitamins C and E administration. All treatments were applied for 4 weeks, respectively. Furthermore, 5 mL of blood samples were collected intracardially. Body weight data were collected for the initial and final weights. From serum samples, SOD, GPx, and CAT were measured using the enzymol method; MDA was measured using the high-performance liquid chromatography method; and testosterone and cortisol were measured using the enzyme-linked immunosorbent assay method. All variables were analyzed statistically using analysis of variance followed by the Duncan test (p<0.05). Results: Our findings showed that the T1 and T3 groups significantly decreased (p<0.001) compared to T2 in the following parameters: SOD, MDA, GPx, and cortisol. Meanwhile, CAT and testosterone levels in the T1 and T3 groups were significantly increased (p<0.001) compared to the T2 group. In addition, the weight gain in T1 and T3 groups was significantly increased (p<0.001) compared to T2 group. Conclusion: It can be concluded that the administration of Vitamins C and E had a significant effect to alleviate SOD, MDA, GPx, and cortisol and to improve the testosterone level in albino rats with CVS.

scholarly journals ISOLASI DAN ANALISIS GENISTEIN DARI TEMPE BUSUK MENGGUNAKAN METODE KROMATOGRAFI KOLOM

2018 ◽  
Vol 5 (1) ◽  
pp. 88
Author(s):  
Hartati Soetjipto ◽  
Yohanes Martono ◽  
Zulfa Yuniarti
Keyword(s):  
Column Chromatography ◽  
Crude Extract ◽  
Mobile Phase ◽  
High Performance ◽  
Extraction Methods ◽  
Chromatography Method ◽  
Mobile Phases ◽  
Phase Combination ◽  
Layer Chromatography

Isolation and Analysis of Genistein of Overripe Tempe using Column Chromatography MethodABSTRACTGenistein is one of the aglycone isoflavone compounds in tempe that has various biochemical activities, including anticancer, antitumor, and antioxidants. Commonly used isoflavone extraction methods resulted in isoflavone crude extract. The aim of this study was to isolate the genistein of overripe tempe through determining the appropriate combination of mobile phases in genistein isolation and the determination of genistein content in both crude extract and isolate. The overripe tempe was first extracted, then genistein was isolated from the crude extract using column chromatography method. The determination of mobile phase combination was done by Thin Layer Chromatography while the genistein content was quantitatively determined by using High Performance Liquid Chromatography. The results showed that the appropriate combination of mobile phase for genistein isolation was chloroform : methanol (15 : 1, v/v). Genistein content in the crude extract and isolates were 4737.50 and 31.36 μg/g extract, respectively. The genistein purity in the isolates was 63.80%, while the purity in the isoflavone extract was 31.98%.Keywords: genistein, HPLC, isoflavone, overripe tempe, TLC ABSTRAKGenistein merupakan salah satu senyawa isoflavon aglikon dalam tempe yang memiliki bermacam-macam aktivitas biokimia, diantaranya antikanker, antitumor, dan antioksidan. Metode ekstraksi isoflavon yang umum diterapkan, menghasilkan ekstrak kasar isoflavon yang masih berupa campuran. Tujuan dari penelitian ini adalah untuk mengisolasi genistein dari tempe busuk melalui tahap penentuan kombinasi fase gerak yang tepat dalam isolasi genistein serta penentuan kandungan genistein baik dalam ekstrak kasar maupun isolat. Tempe busuk mula-mula diekstrak, selanjutnya genistein diisolasi dari ekstrak kasar menggunakan metode kromatografi kolom. Penentuan kombinasi fase gerak dilakukan secara Kromatografi Lapis Tipis, sedangkan kandungan genistein secara kuantitatif ditentukan dengan menggunakan Kromatografi Cair Kinerja Tinggi. Hasil penelitian menunjukkan bahwa kombinasi fase gerak yang tepat untuk isolasi genistein adalah kloroform : metanol (15 : 1, v/v). Kandungan genistein dalam ekstrak kasar dan isolat genistein berturut-turut sebesar 4737,50 dan 31,36 μg/g ekstrak. Kemurnian genistein dalam isolat adalah sebesar 63,80%, sedangkan kemurniannya dalam ekstrak isoflavon adalah sebesar 31,98%. Kata Kunci: genistein, HPLC, isoflavon, tempe busuk, KLT

Sign in / Sign up

Export Citation Format

Share Document