Analysis Of Types Of Carotenoid Pigments In Crab Sesarmops sp From Manado By Coast

Carotenoid pigments are a group of pigments that are yellow, orange, and red-orange in color. Pigments are natural dyes found in plants and animals, extracts of carotenoid pigments can be separated by chromatographic methods where the common chromatographic methods in determining the type of pigment are Column Chromatography (CC) and Thin Layer Chromatography (TLC). The purpose of this study was to determine the types of pigments contained in the carapace extract of the male crab Sesarmops sp. The Sesarmops sp crab has a brown dorsal carapace with blackish-brown leg spots known as mangrove crabs. Crabs in the Sesarmidae family have a carapace formation and wide legs, do not have swimming legs, and have a pair of claws that are faded purple, the presence of these colors can be identified as containing carotenoid pigments. The results of this study obtained the content of carotenoid pigment 25.2 g/gr and the value of the concentration of carotenoid pigment in the male crab extract Sesarmops sp 10.99 g. The results of the separation of the total pigment extract using column chromatography obtained the types of pigments -carotene, Ekinenon, Zeaxanthin, and Astaxanthin. Keywords: Column Chromatography (CC); Carotenoids; Sesarmops sp AbstrakPigmen karotenoid adalah sekelompok pigmen yang berwarna kuning, oranye dan merah oranye. Pigmen adalah zat warna alami yang terdapat pada tumbuhan dan hewan, ekstrak pigmen karotenoid dapat dipisahkan dengan metode kromatografi yang dimana metode kromatografi umum dalam menentukan jenis pigmen adalah Kromatografi Kolom (KK) dan Kromatografi Lapis Tipis (KLT). Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen apa saja yang terdapar pada ekstrak karapas kepiting jantan Sesarmops sp. Kepiting Sesarmops sp memiliki karapas dorsal bewarna coklat dengan bintik kaki coklat kehitaman yang dikenal sebagai kepiting mangrove. Kepiting dalam keluarga sesarmidae memiliki bentukan karapas dan kaki yang lebar tidak memiliki kaki renang serta memiliki sepasang capit berwarna ungu pudar, adanya warna tersebut kepiting dapat diidentikasikan mengandung jenis pigmen karotenoid. Dari hasil penelitian ini mendapatkan kandungan pigmen karotenoid 25,2 dan nilai konsentrasi pigmen karotenoid pada ekstrak kepiting jantan Sesarmops sp 10,99 . Hasil pemisahan dari ekstrak pigmen total menggunakan kromatografi kolom didapatkan jenis pigmen β-karoten, Ekinenon, Zeaxantin dan Astaxantin

Analysis of Carotenoid Pigment Types in the Carapace of the Male Crab Grapsus albolinetaus Latreille in Milbert 1812

Carotenoid pigments have various colors such as yellow, orange, or red-orange. One of the carotenoid pigment sources is Crab G. albolineatus Latreille in Milbert 1812. Column chromatography separation technique was used to determine the metabolism of carotenoid pigments in the crab G. albolineatus latreille in Milbert 1812. This CC separation used hexane and acetone as the developer solution (70:30). The developer solution as known as the mobile phase is semipolar, while the stationary phase is silica powder G60. Therefore, it formed two metabolic pathways. The male G. albolineatus crab used in this study was on the D3 molting stage which had a concentration of 36.37 g/g dry residue and 4.72 g content. The types of pigments identified are: β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Keywords: Carotenoid Pigments; G. albolineatus; Column Chromatography; MoltingAbstrakPigmen karotenoid memiliki berbagai warna seperti kuning, oranye, atau merah oranye. Salah satu sumber pigmen karotenoid adalah pada karapas kepiting G. albolineatus Latreille in Milbert 1812. Untuk mengetahui metabolisme jenis pigmen karotenoid pada kepiting Grapsus albolineatus latreille in Milbert 1812 yaitu menggunakan pemisahan kromatografi Kolom. Pemisahan KK ini menggunakan larutan pengembang heksan dan aseton (70:30). Larutan pengembang merupakan fase gerak yang bersifat semipolar dan fase diamnya menggunakan bubuk silika G60. Terbentuk dua jalur metabolisme. Kepiting G. albolineatus jantan yang digunakan pada penelitian berada di stadium molting D3 dengan konsentrasi sebesar 36,37 µg/g berat residu kering dan kandungan 4,72 µg. Jenis pigmen yang teridentifikasi yaitu : β – karoten, Zeaxanthin, lutein, β – kriptoxanthin dan Astaxanthin.Kata kunci: Pigmen; Karotenoid; G. albolineatus; Kromatofrafi Kolom; Molting

Reproductive Biology of Land Crabs (Gecarcoidea lalandii) in Ly Son Island, Quang Ngai Province – Vietnam

The reproductive biology of the land crab Gecarcoidea lalandii was documented for the Ly Son Island, Quang Ngai Province, Vietnam. Approximately 30 crabs, collected every month in the 12-month period from August 2019 to July 2020, were analyzed gonadal development stages, sex ratio, spawning season, fecundity and size at first sexual maturity. Results showed that number of male and female crab in nature was not significantly different. Gonadal of the crab passed through five stages of maturation. The crab spawns continuously during the spawning season. In nature, the crab can mature whole year-round with a higher proportion from January to August and the peak maturation from June to July. The spawning season is of this species is only on the raining period from June to July. Length at first sexual maturity of the crab was 53.91 mm of carapace width for female and 50.39 mm male crab. The absolute fecundity varied in 473,000-820,000 eggs ind-1 and highly related to body weight. The current findings provide the scientific foundation for fishery resource management and artificial breeding of the land crab in Ly Son.

Growth and survival of Mud Crab, Scylla serrata, reared in bottom and floating cages within Mida Creek mangroves, coastal Kenya

Mud Crab fattening is a relatively new business idea in Kenya and is highly lucrative due to the reasonable prices offered at tourist hotels. This study was aimed at developing appropriate culture techniques for Mud Crabs to maximize harvestable biomass by the local communities and avoid recruitment overfishing. Growth and survival rates for mixed sex Mud Crabs was investigated for a period of 230 days in Mida Creek, Kenya, using bottom and floating cages, and two feed types in a crab fattening experiment. Crabs attained harvestable weight by the third month for both floating (466.2 ± 137 g) and bottom cages (542.2 ± 109.3 g). There was no significant difference in mean total weight of mixed-sex crabs in the two culture systems (t = 1.75, p>0.05), however males showed significantly higher total weight gain compared to females. A higher overall survival rate was found for crabs cultured in floating cages (63.8%), compared to bottom cages (44.9%). There was no significant difference in growth performance between crabs fed gastropod tissues and those fed fish offal. The long-term specific growth rate for floating cages (0.69 g d-1) was not significantly different from that of bottom cages (0.92 g d-1). An analysis of potential return on investment showed the floating cages to be more profitable per production cycle compared to the bottom ones. The floating cage system and mono-sex male crab culture are recommended to farmers within mangrove tidal flats in Kenya.

Biologi Rajungan Portunus pelagicus Linnaeus, 1758 (Crustacea: Portunidae) Ditinjau Dari Aspek Distribusi Ukuran Dan Parameter Pertumbuhan Di Perairan Rembang, Jawa Tengah

Rembang merupakan salah satu kabupaten di Jawa Tengah yang dikenal sebagai daerah penghasil sumberdaya rajungan yang cukup tinggi permintaannya dan terus meningkat setiap tahunnya. Hal tersebut dapat menyebabkan terjadinya penurunan potensi hasil rajungan akibat intensifnya penangkapan di Perairan Rembang. Kajian tentang kondisi stok sumberdaya rajungan terkait sebaran dan dinamika populasi ini sangat diperlukan dalam pengelolaan potensi rajungan. Tujuan dari penelitian ini adalah untuk menganalisis struktur ukuran dan parameter pertumbuhan rajungan di Perairan Rembang. Penelitian ini dilaksanakan pada bulan Maret – Juni 2019 di Perairan Rembang, dengan menggunakan metode survei yang bersifat deskriptif. Total sampel rajungan selama penelitian adalah 1200 ekor yang terdiri dari 574 ekor jantan dan 624 ekor betina. Hasil penelitian menunjukkan kelas ukuran lebar karapas rajungan didominasi oleh rajungan muda, dengan rata – rata 105.01 ± 11.99 mm untuk jantan dan 107.73 ± 10.68 untuk betina. Berat rata – rata rajungan jantan yaitu sebesar 95.2 ± 27.1 gram dan rajungan betina sebesar 97.9 ± 23.1 gram, sehingga menunjukkan rajungan jantan lebih kecil dibandingkan dengan rajungan betina. Pola pertumbuhan rajungan di Perairan Rembang bersifat allometrik negatif. Laju pertumbuhan rajungan di Perairan Rembang yang didapatkan adalah berupa nilai panjang asimptotik (L∞) sebesar 71.4 mm dengan koefisien pertumbuhan (K) sebesar 1.5/tahun.Rembang is a coastal region in Central Java which known as to producer of blue swimming crab resources that are in high demand and continues to increase every year. It will because decreasing the potential yield of blue swimming crab due to intensive fishing in Rembang Waters. Study on the stock condition of the blue swimming crab resource related to the distribution and dynamics of the population is required for the management of the blue swimming crab potential. The current research aims to assess the size structure and growth parameter of blue swimming crab in the Rembang Waters. The research was conducted from March to June 2019 in Rembang Waters, using a descriptive survey method. The total number of crab samples during the study was 1200 animals consisting of 574 male and 624 female. The results showed that the size of the crab carapace width class was dominated by young crabs, with an average of 105.01 ± 11.99 mm for males and 107.73 ± 10.68 for females. The average weight of male crab is 95.2 ± 27.1 gram and female crab is 97.9 ± 23.1 gram, indicates that male crab is smaller than female crab. The crab’s growth in Rembang Waters is allometric negative. The growth rate of crab in the Rembang Waters obtained is in the form of asymptotic length (L∞) was 71.4 mm with a growth coefficient (K) 1.5/year.

Distribusi Ukuran dan Tingkat Kematangan Gonad Portunus pelagicus, Linnaeus, 1758 (Malacostraca : Portunidae) di Perairan Rembang, Jawa Tengah

ABSTRAK : Rajungan merupakan komoditas ekspor bernilai ekonomis penting dan memiliki nilai komersial yang tinggi. Tingginya permintaan pasar terhadap komoditas perikanan rajungan memicu eksploitasi yang berlebihan sehingga dapat berdampak terhadap kelestarian sumber daya rajungan. Penelitian ini bertujuan untuk mengetahui distribusi ukuran, hubungan lebar dan berat, distribusi TKG, serta nisbah kelamin rajungan (Portunus pelagicus) di Perairan Gegunung Wetan, Rembang. Penelitian ini terdiri dari pengukuran panjang dan lebar karapas, pengamatan tingkat kematangan gonad rajungan betina, serta pengamatan kualitas air (salinitas, suhu, pH, kedalaman dan kecerahan). Hasil dari penelitian ini menujukkan dari 3000 ekor rajungan yang diamati diketahui distribusi lebar karapas rajungan berkisar antara 7,3 – 16 cm dengan kisaran berat sebesar 47–262 gram. Rajungan yang tertangkap memiliki pertumbuhan yang bersifat allometrik negatif, dengan nilai b sebesar 2,12 pada rajungan jantan, 1,65 pada rajungan betina. Faktor kondisinya adalah 1,1 pada jantan dan 1,02 pada betina. Sedangkan distribusi tingkat kematangan gonad rajungan betina adalah 762 ekor pada TKG 1; 700 ekor pada TKG 2; serta 388 ekor pada TKG 3.ABSTRACT : Blue swimming crab has a high economical value. The production of blue swimming crab is exported overseas, so, therefore, triggers the excessive exploitation. This will lead to have an impact related to the sustainability of natural resource. This research aim to find out the size distribution, the relationship of carapace widht and body weight, gonad maturity stages distribution and sex ratio of blue swimming crab (P. pelagicus) at Gegunung Wetan Waters, Rembang. This consider to have a role as a tool on planning the farming and managing the blue swimming crab conservation. This research cover the length and width measurement of blue swimming crab carapace, gonad maturity stages observation of female blue swimming crab and water quality measurments (salinity, temperature, pH, water depth and water brightness). The result showed that 3000 blue swimming crabs have 7.3-16 cm of widht carapace distribution and 47-262 gram of average weight. It can be concluded that growth are negatively allometric with b value (2,12) for the male crabs and b value (1.65) for female crabs.. The condition factor of male crab is 1,1 and female crabs is 1,02. Based on the research, the result also shows that gonad maturition stages of female crabs were 762 crabs for stage 1, 700 crabs for stage 2 and 388 crabs for stage 3.

ANALISIS HASIL TANGKAPAN RAJUNGAN (PORTUNUS PELAGICUS) SEBAGAI TARGET UTAMA DAN KOMPOSISI BY-CATCH ALAT TANGKAP GILLNET DI PERAIRAN PANTAI PURIRANO, SULAWESI TENGGARA

Abstrak : Penangkapan rajungan di perairan Pantai Purirano umumnya dilakukan oleh usaha perikanan rajungan skala kecil, yang menggunakan jaring insang (gillnet). Gillnet merupakan alat tangkap pasif yang pengoperasiannya tidak merusak sumberdaya hayati perairan. Walaupun demikian, gillnet merupakan alat tangkap yang tingkat selektivitasnya rendah, sehingga dikhawatirkan hasil tangkapan sampingan (bycatch) lebih banyak daripada hasil tangkapan utama (target species). Penelitian ini bertujuan untuk mengetahui proporsi hasil tangkapan rajungan (portunus pelagicus) sebagai target utama dan komposisi bycatch alat tangkap gillnet di perairan Pantai Purirano.Sampel rajungan ditangkap menggunakan jaring insang (gillnet) dengan ukuran mata jaring 4 inci & lebar jaring 80 cm. Hasil tangkapan selama penelitian diperoleh 29 jenis dari 25 famili. Hasil tangkapan yang diutamakan adalah rajungan dari famili Portunidae, tetapi terdapat juga jenis-jenis lain yang juga tertangkap (bycatch), yang sebagian dimanfaatkan (useable) dan sebagian lain dibuang ke laut (discarded). Jumlah total hasil tangkapan rajungan jantan selama penelitian adalah 58 ekor (54%) dan betina sebanyak 50 ekor (46%). Indeks dominansi hasil tangkapan tergolong rendah yaitu berkisar 0,21 – 0,27. Hal ini mengindikasikan bahwa selektivitas alat tangkap jaring insang tergolong rendah.Kata Kunci : Rajungan (Portunus pelagicus), Bycatch, Gillnet, PuriranoAbstrack : Catching crabs in Purirano Beach is generally carried out by small-scale crab fishing businesses, which use gillnet. Gillnet is a passive fishing gear whose operation does not damage aquatic biological resources. However, gillnet is a fishing tool with a low selectivity level, so it is feared that bycatch by-catch is more than the main catch (target species). This study aims to determine the proportion of crab catches (portunus pelagicus) as the main target and the composition of gillnet fishing gear bycatch in Purirano Beach waters. Swimming crab samples were captured using gill nets with mesh sizes of 4 inches & net width of 80 cm. The catch during the study obtained 29 species from 25 families. The preferred catch is crabs from the family Portunidae, but there are also other species that are also caught (bycatch), some are used (useable) and some others are thrown into the sea (discarded). The total number of male crab catches during the study was 58 individuals (54%) and 50 females (46%). The catch index dominance is relatively low, in the range of 0.21 - 0.27. This indicates that the selectivity of gill nets is relatively low.Keywords : Rajungan (Portunus pelagicus), Bycatch, Gillnet, Purirano

PARAMETER POPULASI DAN TINGKAT PEMANFAATAN KEPITING BAKAU (Scylla serrata Forsskal, 1775) DI PERAIRAN SEBATIK, KALIMANTAN UTARA

Salah satu informasi yang diperlukan untuk merumuskan pengelolaan kepiting bakau (Scylla serrata Forsskal, 1775) yang berkelanjutan adalah parameter populasi dan tingkat pemanfaatannya. Penelitian ini bertujuan untuk mengkaji parameter populasi dan tingkat pemanfaatan kepiting bakau di perairan Sebatik. Penelitian dilakukan pada Maret-Desember 2017. Sampel kepiting bakau di peroleh dari hasil tangkapan nelayan dengan alat tangkap bubu di perairan Sebatik. Pertumbuhan dianalisis berdasarkan persamaan Von Bertalanffy dengan mengamati pergeseran struktur ukuran kepiting tiap bulan dan tingkat pemanfaatan diperoleh dari metode kurva konversi panjang dengan hasil tangkapan. Hasil penelitian menunjukkan kepiting bakau yang tertangkap bubu di perairan Sebatik berukuran lebar karapas (carapace width) antara 84-144 mmCW dengan rata-rata ukuran yang tertangkap adalah 107,05±12,3 mmCW pada kepiting jantan dan 110,2±8,86 mmCW pada kepiting betina. Pertumbuhan berat kepiting bakau jantan lebih cepat dibandingkan ukurannya (b=3,6) sebaliknya pertumbuhan berat kepiting bakau betina lebih lambat dibandingkan ukurannya (b=2,5). Nisbah kelamin kepiting bakau menunjukkan kondisi tidak seimbang (5,5 : 1) dengan proporsi jantan lebih dominan dibandingkan betina. Lebar karapas asimptotik (CW) kepiting bakau jantan adalah 151,2 mmCW dan betina adalah 140,5 mmCW. Laju pertumbuhan (K) kepiting bakau adalah 0,75 tahun-1 pada kepiting jantan dan 0,79 tahun-1 pada kepiting betina. Status pemanfaatan kepiting bakau telah berada dalam tahapan mendekati lebih tangkap (E=0,5-0,55) sehingga disarankan tidak melakukan penambahan upaya penangkapan kepiting bakau di perairan Sebatik. One of the information needed for formulating the sustainable management of mud crab is the availability of information on the population parameters and its exploitation rate. The current research aimed to study the population parameters and the exploitation rate of mud crab in Sebatik Waters. Field research was conducted in March – December 2017. Samples of mud crab were obtained from the catch of fisherman by trap in Sebatik Waters. The Von Bertalanffy growth parameters were constructed through monthly modals progression analysis of the size of carapace width frequencies distributions and the exploitation rate was estimated through the method of length converted catch curve. The results showed that the carapace width of mud crabs caught by trap in Sebatik Waters ranged between 84 to 144 mmCW with the mean size of 107.05±12.3 mmCW for male and 110.2±8.86 mmCW for female. The growth of weights of male crab (b=3.6) is faster than its size, while the growth of weight of female crab was slower than its size (b=2,5). The sex ratio of mud crab was unbalanced (5.5 : 1) that the proportion of male was more dominant than female. Asymptotic carapace width (CW) of mud crab was 151.2 mmCW for male and 140.5 mmCW for female. The growth rate (K) of mud crab was 0.75 year-1 for male and 0.79 year-1 for female. The exploitation status of mud crab was starting to overfishing (E=0,5-0,55) so it suggest to not increase the fishing effort of mud crab fishery in Sebatik Waters.

The Amino Acid Composition of Blue Swimming Crab (Portunus Segnis, Forskal, 1775) from The North Eastern Mediterranean Sea of Turkey

This study was carried out to detect the content of amino acid in female and male specimens of blue swimming crab (Portunus segnis) obtained from (including 12 male crab and also 12 female crab) North Eastern Mediterranean Sea, Turkey. The protein was identified as 17.63% and 18.13% for female and male crab respevtively. Totally 9 essential amino acids were recorded in the present study. Lysine and leucine constituted the highest essential amino acid (EAA) concentrations in P. segnis. This species was found to be in good score in terms of the level of EAA and the EAA/Non-EAA ratios when compared with other economical crab species.