scholarly journals ALLELIC VARIATIONS OF SOS, HVNHX AND SDO GENES IN SOME BARLEY (Hordeum Vulgare L.) GENOTYPES

2021 ◽  
Vol 52 (6) ◽  
pp. 1441-1448
Author(s):  
D. Taher ◽  
A. AL-Aouda ◽  
S. Lawand
Keyword(s):  
Hordeum Vulgare L ◽  
Morphological Pattern ◽  
Field Crops ◽  
Polymorphic Pattern ◽  
Amplicon Size ◽  
Allelic Variations ◽  
High Degree ◽  
Per Gene ◽  
Very High ◽  
Barley Genotypes

This study has been carried out at the Biotechnology Lab., Department of Field Crops, Faculty of Agriculture, Damascus University during the growing season 2019 - 2020, in order to detect the variations of SOS, HVNHX and SDO genes in different barley genotypes.  Clear variations in the SOS, HVNHX and SDO genes, which are responsible for salinity tolerance were found among the investigated genotypes. It has been found that the variation in the amplicon size between loci per gene was very high in some cases, while there was a high degree of symmetry in other cases, and could be easily distinguished on 2% Agarose gel. The PCR results for the SOS genes (SOS1, SOS2, SOS3), HVNHX genes (HVNHX1, HVNHX2, HVNHX3) and SDO genes (Cu/Zn SODII, Cu/Zn SODI, CAT, GRI , APXIII) have shown only one morphological pattern in most of the studied genotypes, while revealed two patterns for the SOS3 gene, but the rest of genes (HVNHX1, HVNHX2, HVNHX3  Cu/Zn SODI, CAT) exhibited only one morphological pattern. The SOS3 was superior in the number of polymorphic patterns, as the number of total patterns was 14 in all the studied genotypes, but the Cu/Zn SODI showed the least number of polymorphic patterns with only 1 pattern, while the largest number (7 patterns) was detected in the genotype (H9), but the two genotypes Fourat9 and Fourat7 showed only one polymorphic pattern.

scholarly journals Y A-T-Il Une Relation Entre Teneur En Chlorophylle Et Accumulation De La Proline Chez Les Céréales À Paille Sous Déficit Hydrique?

2018 ◽  
Vol 14 (15) ◽  
pp. 234
Author(s):  
Merabta Sarra ◽  
Zerafa Chafia ◽  
Benlaribi Mostefa
Keyword(s):  
Chlorophyll Content ◽  
Triticum Aestivum L ◽  
Hordeum Vulgare L ◽  
Test Conditions ◽  
Triticum Durum Desf ◽  
The Moment ◽  
Déficit Hydrique ◽  
The Relationship ◽  
Very High ◽  
Barley Genotypes

This paper focuses on the relationship between two genotypes of durum wheat (Triticum durum Desf.): Gamgoum Rekham (GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence aurore 8193 (FA) and Mexipak; and two genotypes of barley (Hordeum vulgare L.): Manel and Saïda 183. These genotypes were subjected to a water deficit during a period of twenty days at the heading stage. The proline content and the chlorophyll content are determined on the standard leaves both on the control and on the sample subjected to water stress after twenty days and after one and two weeks, following the return of watering. Despite the fact that these varieties are cultivated under the same conditions on the Algerian highlands, their reactions to the test conditions are very different. As a matter of fact, GGR and FA recorded very high levels of proline at the end of the stress. This, however, was at the moment when the two barley genotypes presented relatively low values for the two studied parameters. After the return of watering, the proline contents at the various genotypes returned gradually to those of the controls. The GGR genotype recovers more quickly than the others. As for the chlorophyll content, it evolves, unevenly, in the course of the test, showing no evolutionary indication in relation to the proline.

Electron Microscopy in Molecular Biology

1967 ◽  
Vol 25 ◽  
pp. 2-3
Author(s):  
Cecil E. Hall
Keyword(s):  
Electron Microscopy ◽  
Molecular Biology ◽  
Noise Level ◽  
Molecular Structures ◽  
Material Structure ◽  
The Arts ◽  
Shadow Casting ◽  
Electron Image ◽  
High Degree ◽  
Very High

The visualization of organic macromolecules such as proteins, nucleic acids, viruses and virus components has reached its high degree of effectiveness owing to refinements and reliability of instruments and to the invention of methods for enhancing the structure of these materials within the electron image. The latter techniques have been most important because what can be seen depends upon the molecular and atomic character of the object as modified which is rarely evident in the pristine material. Structure may thus be displayed by the arts of positive and negative staining, shadow casting, replication and other techniques. Enhancement of contrast, which delineates bounds of isolated macromolecules has been effected progressively over the years as illustrated in Figs. 1, 2, 3 and 4 by these methods. We now look to the future wondering what other visions are waiting to be seen. The instrument designers will need to exact from the arts of fabrication the performance that theory has prescribed as well as methods for phase and interference contrast with explorations of the potentialities of very high and very low voltages. Chemistry must play an increasingly important part in future progress by providing specific stain molecules of high visibility, substrates of vanishing “noise” level and means for preservation of molecular structures that usually exist in a solvated condition.

A Low-Noise, High-Gain Quasi-Millimeter-Wave Receiver MMIC with a Very High Degree of Integration Using 3D-MMIC Technology

2011 ◽  
Vol E94-C (10) ◽  
pp. 1548-1556 ◽  
Author(s):  
Takana KAHO ◽  
Yo YAMAGUCHI ◽  
Kazuhiro UEHARA ◽  
Kiyomichi ARAKI
Keyword(s):  
Millimeter Wave ◽  
High Gain ◽  
Low Noise ◽  
High Degree ◽  
Very High ◽  
Millimeter Wave Receiver

scholarly journals Multi-Locus Selection and the Structure of Variation at the white Gene of Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 635-645 ◽  
Author(s):  
David A Kirby ◽  
Wolfgang Stephan
Keyword(s):  
Drosophila Melanogaster ◽  
White Gene ◽  
Transcriptional Unit ◽  
North American Population ◽  
Evolutionary Mechanisms ◽  
Locus Selection ◽  
Neutral Equilibrium ◽  
History Of ◽  
High Degree ◽  
Very High

Abstract We surveyed sequence variation and divergence for the entire 5972-bp transcriptional unit of the white gene in 15 lines of Drosophila melanogaster and one line of D. simulans. We found a very high degree of haplotypic structuring for the polymorphisms in the 3′ half of the gene, as opposed to the polymorphisms in the 5′ half. To determine the evolutionary mechanisms responsible for this pattern, we sequenced a 1612-bp segment of the white gene from an additional 33 lines of D. melanogaster from a European and a North American population. This 1612-bp segment encompasses an 834bp region of the white gene in which the polymorphisms form high frequency haplotypes that cannot be explained by a neutral equilibrium model of molecular evolution. The small number of recombinants in the 834bp region suggests epistatic selection as the cause of the haplotypic structuring, while an investigation of nucleotide diversity supports a directional selection hypothesis. A multi-locus selection model that combines features from both-hypotheses and takes the recent history of D. melanogaster into account may be the best explanation for these data.

The combined effect of nitrogen fertilizer and sowing season on response to water-limited stress in barley (Hordeum vulgare L.)

2021 ◽  
pp. 1-19
Author(s):  
S. Bardehji ◽  
H. R. Eshghizadeh ◽  
M. Zahedi ◽  
M. R. Sabzalian ◽  
M. Gheisari
Keyword(s):  
Block Design ◽  
Soluble Carbohydrate ◽  
Total Biomass ◽  
Nitrogen Application ◽  
Hordeum Vulgare L ◽  
High Positive Correlation ◽  
Irrigation Level ◽  
Sowing Season ◽  
Response To Water ◽  
Barley Genotypes

Abstract A field experiment was carried out for over two seasons (autumn and spring) as a split–split plot scheme based on a randomized complete block design with three replications. The main plots included two irrigation levels of the maximum available water depletion (maximum allowable depletion (MAD)) of 55 and 85% as non-stress and drought-stress environments, respectively, and the subplot accommodated two levels of nitrogen (0 and 62.5 kg N/ha, urea fertilizer); also, 20 barley genotypes were assigned to the sub-subplots. The biplot analysis of both sowing seasons showed that grain yield (GY) had a high positive correlation with total biomass (TB), whereas it had a high negative correlation with proline and total soluble carbohydrate as drought-tolerance-determinant characteristics. The genotypes which had the lowest and highest GY ranked significantly (P ≤ 0.01) different with changing the sowing season under each irrigation level, indicating a larger plant interaction and non-stability in response to the season change (about two-fold), as compared to the change in the irrigation conditions. It could also be concluded that barley genotypes might experience a higher decrease in GY and sensitivity to water deficit in the autumn sowing season, as compared to the spring planting season, which was also intensified by nitrogen application. However, the response to nitrogen application depends on the plant genotype.

scholarly journals Comparative assessment of genetic diversity in wild and primitive cultivated barley in a center of diversity.

Genetics ◽  
1988 ◽  
Vol 119 (4) ◽  
pp. 981-990
Author(s):  
S Jana ◽  
L N Pietrzak
Keyword(s):  
Genetic Diversity ◽  
Eastern Mediterranean ◽  
Hordeum Spontaneum ◽  
Hordeum Vulgare L ◽  
Closely Related Species ◽  
Mediterranean Countries ◽  
Isozyme Loci ◽  
Cultivated Barley ◽  
Multilocus Associations ◽  
High Degree

Abstract Wild barley (Hordeum spontaneum K.) and indigenous primitive varieties of cultivated barley (Hordeum vulgare L.), collected from 43 locations in four eastern Mediterranean countries, Jordan, Syria, Turkey and Greece, were electrophoretically assayed for genetic diversity at 16 isozyme loci. Contrary to a common impression, cultivated barley populations were found to maintain a level of diversity similar to that in its wild progenitor species. Apportionment of overall diversity in the region showed that in cultivated barley within-populations diversity was of higher magnitude than the between-populations component. Neighboring populations of wild and cultivated barleys showed high degree of genetic identity. Groups of 3 or 4 isozyme loci were analyzed to detect associations among loci. Multilocus associations of varying order were detected for all three groups chosen for the analysis. Some of the association terms differed between the two species in the region. Although there was no clear evidence for decrease in diversity attributable to the domestication of barley in the region, there was an indication of different multilocus organizations in the two closely related species.

Continuous DNA replication in the nucleus of the dinoflagellate Prorocentrum micans (Ehrenberg)

1977 ◽  
Vol 27 (1) ◽  
pp. 81-90
Author(s):  
S.A. Filfilan ◽  
D.C. Sigee
Keyword(s):  
Electron Microscope ◽  
Dna Synthesis ◽  
Nuclear Dna ◽  
Continuous Process ◽  
Prorocentrum Micans ◽  
Interphase Nuclei ◽  
Electron Microscope Autoradiography ◽  
Dividing Cells ◽  
High Degree ◽  
Very High

The uptake of tritiated thymine into cells of a heterogeneous population of Prorocentrum micans was investigated using light-microscope and electron-microscope autoradiography. Specificity of thymine uptake into DNA was demonstrated by the specific removal of label from wax-embedded material using DNase and by the high degree of localization of nuclear label to chromosomes in the electron-microscope autoradiographs. All nuclei, including both dividing and non-dividing cells, showed a substantial uptake of label, indicating that nuclear DNA synthesis in Prorocentrum micans is a continuous process. The level of DNA synthesis does show considerable variation, however, with very high levels in some interphase nuclei. The continuous replication of nuclear DNA provides further evidence of dinoflagellate affinity to the prokaryotes, and indicates that Prorocentrum micans is a very primitive eukaryote cell.

Decision Support System Based on Fuzzy Logic for Assessment of Expected Corporate Income Performance

2021 ◽  
pp. 2150009
Author(s):  
Arthur Yosef ◽  
Eli Shnaider ◽  
Rimona Palas ◽  
Amos Baranes
Keyword(s):  
Fuzzy Logic ◽  
Decision Support ◽  
Operating Income ◽  
Explanatory Variables ◽  
Section Model ◽  
Evaluation Mechanism ◽  
One Year ◽  
High Degree ◽  
Decision Support Method ◽  
Very High

This study presents a decision-support method to estimate the next year performance of corporate Operating Income Margin (OIM). It is based on a unique combination of cross-section model and the rules-based evaluation mechanism. The estimate is done in terms of broad categories, and not precise numerical values. The model is constructed as follows: its dependent variable (OIM) is one year ahead vs. the corresponding explanatory variables. This structure of the model allows us to view explanatory variables as reflecting financial potential of corporations. The evaluation component consists of a set of rules designed to identify the companies whose “potential” clearly points to an opportunity to invest. For the method presented here to succeed, it is necessary to utilize a highly reliable modeling method, even if it is “Fuzzy”. We apply Soft Regression (SR), which is a Soft Computing modeling tool based on Fuzzy Logic, and utilize all available proxy variables by creating intervals of values. Advantages of utilizing SR, and the intervals’-based modeling are extensively discussed. Modeling results for five consecutive years are consistent and stable, thus indicating high degree of reliability. Testing indicates very high success rate for the stock market related domain, the lowest being 87.9%.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 525-534 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Dna Synthesis ◽  
Tritiated Thymidine ◽  
Amoeba Proteus ◽  
Living Cells ◽  
Large Numbers ◽  
Cytoplasmic Dna ◽  
Basic Dyes ◽  
Nuclease Digestion ◽  
High Degree ◽  
Very High

The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detectable structures. Staining experiments with basic dyes, including the fluorochrome acridine orange, revealed the presence of large numbers of 0.3 to 0.5 µ particles in the cytoplasm of all cells studied. The effect of nuclease digestion on the dye affinity of the particles suggests that they contain DNA as well as RNA. Centrifugation of living cells at 10,000 g leads to the sedimentation of the particles in the centrifugal third of the ameba near the nucleus. Analysis of centrifuged cells which had been incubated with H3-thymidine showed a very high degree of correlation between the location of the nucleic acid-containing granules and that of acid-insoluble, deoxyribonuclease-sensitive labeled molecules and leads to the conclusion that cytoplasmic DNA synthesis in Amoeba proteus occurs in association with these particles.

Social Pathology and Personality in Attempted Suicide

1967 ◽  
Vol 113 (505) ◽  
pp. 1405-1406 ◽  
Author(s):  
A. E. Philip ◽  
J. W. McCulloch
Keyword(s):  
Juvenile Delinquency ◽  
Ecological Study ◽  
Attempted Suicide ◽  
Local Authority ◽  
Suicidal Attempt ◽  
Social Pathology ◽  
Psychological Measures ◽  
High Degree ◽  
Very High

An ecological study by Philip and McCulloch (1966) demonstrated very high correlations between the incidence of attempted suicide and the rates of incidence for repeated attempted suicide, overcrowding, having been taken into care by the local authority, having had contact as a child with the Royal Scottish Society for the Prevention of Cruelty to Children, and juvenile delinquency. McCulloch and Philip (1968) showed that persons admitted to hospital following a suicidal attempt manifested these variables to a high degree. In this latter study “juvenile delinquency” was replaced by “all offences” as a variable. The present paper reports on the relationships between a scale based on these variables and a number of psychological measures gathered from 84 of the 95 patients in the latter study.

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