scholarly journals MACROSCOPIC AND MICROSCOPIC LESIONS CHANGE OF DERMATITIS DOG WITH TRIGONA SP HONEY TREATMENT

2021 ◽  
pp. 26-28
Author(s):  
I Nyoman Suartha ◽  
Luh Made Sudimartin ◽  
Putu Devi Jayanthi ◽  
Ni Putu Ayu Dewi Wijayanti
Keyword(s):  
Clinical Symptoms ◽  
Layer Structure ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Skin Layer ◽  
Wound Healing Process ◽  
Skin Damage ◽  
Skin Ulcers ◽  
Causative Agents

Dermatitis is a term used to describe inflammation and damage of the layers of the skin. The causative agents are bacterial, fungal, mold, and parasitic infestations. Dermatitis has become problems in various regions in Indonesia and some other countries. Dermatitis often causes an unpleasant and disgusting feeling for the owner. Skin damage occurs to the dermis, thus showing clinical symptoms of hair loss, itching, redness of the skin, skin ulcers, and a bad smell of the animals. This study aims to determine the effect of giving honey from Trigona bees to improve the skin layer structure of patients with dermatitis in dogs and to see the development of skin lesions macroscopically. Research method: 10 dogs diagnosed with complex dermatitis were given fresh honey of 5 ml/day/dog. The treatment is given orally once a day for 35 days. Clinical observation of the wound healing process on the skin was observed every week for 5 weeks, and histopathological observations of the skin were carried out on the 0, 7, and 21 days. The results showed that giving fresh honey was able to repair skin damage with dermatitis lesions. It could be seen from the decrease in symptoms of erythema and the start of hair growth at the site of alopecia lesions. Histologically there was a decrease in the number of inflammatory cells (p<0.05) in the administration of honey. We concluded that Trigona's honey could be used as an herbal ingredient for treating dermatitis in dogs

About the effectiveness of cell technologies in extensive soft tissue defects plasty

2020 ◽  
Vol 22 (3) ◽  
pp. 88-92
Author(s):  
E. Ya. Fistal ◽  
A. G. Popandopulo ◽  
V. V. Soloshenko ◽  
K. N. Movchan ◽  
N. S. Romanenkov ◽  
...  
Keyword(s):  
Healing Process ◽  
Study Groups ◽  
Skin Lesions ◽  
Fibroblast Culture ◽  
Wound Healing Process ◽  
Burn Patients ◽  
Fetal Fibroblast ◽  
Skin Defects ◽  
Cell Technologies ◽  
Fetal Fibroblasts

Abstract. Fetal fibroblast culture transplantation results were evaluated in the treatment of 18 burn victims. Comparison group consisted of 18 burn patients received medical care without cellular technologies utilization. The main comparison parameters in the study groups: the timing of the first stage of autodermoplasty; the number of autodermoplasties during the treatment; hospitalization duration; the newly formed epidermis area estimation. Fetal fibroblast culture transplantation in burn patients with extensive skin defects was performed on average 14,883,56 days after the injury. The timing of the first stage of autodermoplasty did not differ in the main and control groups, not exceeding an average of 19,122,01 days (p=0,48). An average of 2,710,67 surgeries using cell technologies performed in patients of the study group. The use of fetal fibroblasts culture in patients with extensive skin defects reduces the need for autodermoplasty by 1,6 times due to the granulation tissue formation and the epidermal growth beginning 7 days after and complete epidermal formation 14 days after transplantation. Regenerative medicine technologies utilization in patients with extensive skin lesions is possible and appropriate. Due to the fetal fibroblasts culture transplantation a kind of temporary biological coating is formed in the wound. It accelerates the wound healing process phase change from exudation to proliferation and the preparation of skin defects for autodermoplasty, expanding the possibilities of effective patients treatment.

scholarly journals The Role of Chemokines in Wound Healing

2018 ◽  
Vol 19 (10) ◽  
pp. 3217 ◽  
Author(s):  
Anisyah Ridiandries ◽  
Joanne Tan ◽  
Christina Bursill
Keyword(s):  
Wound Healing ◽  
Therapeutic Potential ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Wound Healing Process ◽  
Preclinical Research ◽  
Cxcr4 Antagonist ◽  
Impaired Wound Healing ◽  
Novel Approach ◽  
Healing Wounds

Wound healing is a multistep process with four overlapping but distinct stages: hemostasis, inflammation, proliferation, and remodeling. An alteration at any stage may lead to the development of chronic non-healing wounds or excessive scar formation. Impaired wound healing presents a significant health and economic burden to millions of individuals worldwide, with diabetes mellitus and aging being major risk factors. Ongoing understanding of the mechanisms that underly wound healing is required for the development of new and improved therapies that increase repair. Chemokines are key regulators of the wound healing process. They are involved in the promotion and inhibition of angiogenesis and the recruitment of inflammatory cells, which release growth factors and cytokines to facilitate the wound healing process. Preclinical research studies in mice show that the administration of CCL2, CCL21, CXCL12, and a CXCR4 antagonist as well as broad-spectrum inhibition of the CC-chemokine class improve the wound healing process. The focus of this review is to highlight the contributions of chemokines during each stage of wound healing and to discuss the related molecular pathologies in complex and chronic non-healing wounds. We explore the therapeutic potential of targeting chemokines as a novel approach to overcome the debilitating effects of impaired wound healing.

scholarly journals Therapeutic Efficacy of Artificial Skin Produced by 3D Bioprinting

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5177
Author(s):  
Kwang-Sik Jang ◽  
Soon-Jung Park ◽  
Jong-Jin Choi ◽  
Ha-Na Kim ◽  
Kyung-Mi Shim ◽  
...  
Keyword(s):  
3D Printing ◽  
Healing Process ◽  
Skin Layer ◽  
The Body ◽  
Control Group ◽  
Wound Healing Process ◽  
Therapeutic Effects ◽  
Skin Substitutes ◽  
Printing Technology ◽  
3D Printing Technology

The skin protects the body from external barriers. Certain limitations exist in the development of technologies to rapidly prepare skin substitutes that are therapeutically effective in surgeries involving extensive burns and skin transplantation. Herein, we fabricated a structure similar to the skin layer by using skin-derived decellularized extracellular matrix (dECM) with bioink, keratinocytes, and fibroblasts using 3D-printing technology. The therapeutic effects of the produced skin were analyzed using a chimney model that mimicked the human wound-healing process. The 3D-printed skin substitutes exhibited rapid re-epithelialization and superior tissue regeneration effects compared to the control group. These results are expected to aid the development of technologies that can provide customized skin-replacement tissues produced easily and quickly via 3D-printing technology to patients.

scholarly journals Experimental model of the burn wound topical treatment

2003 ◽  
Vol 3 (4) ◽  
pp. 61-66
Author(s):  
Amra Čabaravdić ◽  
Irfan Zulić ◽  
Zakira Mornjaković ◽  
Mirjana Mijanović ◽  
Fahir Bečić
Keyword(s):  
Animal Model ◽  
Clinical Symptoms ◽  
Healing Process ◽  
Surrounding Tissue ◽  
Control Group ◽  
Wound Healing Process ◽  
Herbal Preparation ◽  
Burn Wound ◽  
Experimental Animals ◽  
Significant Difference

AbstractBACKGROUND AND PURPOSE:Clinical research of drugs is a researching step subsequent to the preclinical studies in experimental animals. The aim of our research was to evaluate animal model of wound healing process after the burninducement and effects of the ointment containing natural plants on the process of burn healing.MATERIAL AND METHODS:Burn wounds were experimentally induced in two species of experimental animals which were treated with topically applied herbal preparation with concomitant monitoring of the healing process. Experimental groups (1) of 15 animals each (mice and rats), while control group (2) of 10 animals each (mice and rats) that were not being treated with herbal ointment. After the hair removal, burn was induced on the back of animals by heated brass seal. Different clinical symptoms including oedema of surrounding tissue, redness, exudation, size of the burn surface, histological and microbiological findings were monitored on the days 1, 3, 7, 14 and 21. A statistically significant difference was observed throughout descriptive statistics and paired Student's t-test.CONCLUSION:Physiological healing processes of the acute burn wound following the topical application of herbal preparation can be monitored on the utilized animal model. A three-week treatment resulted in the 90% of completed epithelization in both animal species, indicating the effectiveness of topically applied herbal preparation.

scholarly journals Role of Growth Factors in Cutaneous Wound Healing: A Review

1993 ◽  
Vol 4 (5) ◽  
pp. 729-760 ◽  
Author(s):  
Christopher P. Kiritsy ◽  
Samuel E. Lynch
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Healing Process ◽  
Repair Process ◽  
Wound Healing Process ◽  
Cutaneous Wounds ◽  
Skin Ulcers ◽  
Molecular Events ◽  
Chronic Skin ◽  
Preclinical Animal Models

The well-orchestrated, complex series of events resulting in the repair of cutaneous wounds are, at least in part, regulated by polypeptide growth factors. This review provides a detailed overview of the known functions, interactions, and mechanisms of action of growth factors in the context of the overall repair process in cutaneous wounds. An overview of the cellular and molecular events involved in soft tissue repair is initially presented, followed by a review of widely studied growth factors and a discussion of commonly utilized preclinical animal models. The article concludes with a summary of the preliminary results from human clinical trials evaluating the effects of growth factors in the healing of chronic skin ulcers. Throughout, the interactions among the growth factors in the wound-healing process are emphasized.

Evaluation of the Surgery Wound Healing Process Using Self–Adaptive Skin Suture or Mechanical Stapler

2016 ◽  
Vol 88 (4) ◽  
Author(s):  
Ewa Sztuczka ◽  
Marek Jackowski ◽  
Wioletta Żukowska
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Early Period ◽  
Skin Layer ◽  
Wound Healing Process ◽  
Skin Closure ◽  
Infected Wound ◽  
Control And Prevention ◽  
Mechanical Stapler ◽  
Self Adaptive

AbstractWound healing is a complex and time-phased process. The occurrence of numerous negative conditions as well as external factors have a significant influence on the risk of potential complications. Preparing the patient for surgery, attention should be paid to a number of factors determining the proper healing process.The aim of the study was to compare the results of the early period of surgical wound healing process with access via laparotomy using techniques, which are self-adaptive sutures and mechanical staplers used for skin closure.Material and methods. The study included 120 patients divided into three groups, according to the degree of wound continence, in accordance with the CDC (Center for Disease Control and Prevention). Exclusion criteria based on objective analysis were applied for patients with a higher risk of complications. In all cases the skin layer was closed with monofilament suture or single-patient use stapler. A ten-day observation of the wound healing process was implemented. The study was randomized.Results. In the case of patients groups identified as a “Clean Wound” and “ Clean / Infected Wound” no significant differences were discovered. In the group “Contaminated/Infected Wound” significantly higher percentage of wound-healing complications were reported (p < 0.05) for which monofilament sutures was used.Conclusions. The study showed, that mechanical stapler is recommended for contaminated/infected surgical wounds due to significantly lower risk of complications. In the case of wounds divided as a “Clean” and “Clean/Infected” type of suturing material has no significant effect on wound healing.

scholarly journals Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration

2019 ◽  
Vol 27 (5) ◽  
pp. 1520-1538 ◽  
Author(s):  
Martina Balli ◽  
Francesca Vitali ◽  
Adrian Janiszewski ◽  
Ellen Caluwé ◽  
Alvaro Cortés-Calabuig ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Molecular Mechanism ◽  
Healing Process ◽  
Skin Lesions ◽  
Wound Healing Process ◽  
Transcriptional Signature ◽  
Beneficial Effects ◽  
Healing Response ◽  
Wound Healing Response

Abstract Defective cell migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective and affordable treatment able to improve wound healing capacity. However, the precise molecular mechanism through which AMG exhibits its beneficial effects remains unrevealed. Herein we show that AMG improves skin re-epithelialization by accelerating the migration of fibroblasts and keratinocytes. More specifically, AMG-treated wounds showed improvement of indispensable events associated with successful wound healing such as granulation tissue formation, organized collagen content, and newly formed blood vessels. We demonstrate that AMG is enriched with a pool of WH-associated growth factors that may provide the starting signal for a faster endogenous wound healing response. This work links the increased cell migration rate to the activation of the extracellular signal-regulated kinase (ERK) signaling pathway, which is followed by an increase in matrix metalloproteinase expression and their extracellular enzymatic activity. Overall we reveal the AMG-mediated wound healing transcriptional signature and shed light on the AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process. In this way, we present a framework for future improvements in AMG therapy for skin tissue regeneration applications.

scholarly journals Wound healing complications after revascularization for moyamoya vasculopathy with reference to different skin incisions

2019 ◽  
Vol 46 (2) ◽  
pp. E12 ◽  
Author(s):  
Güliz Acker ◽  
Nicolas Schlinkmann ◽  
Lucius Fekonja ◽  
Lukas Grünwald ◽  
Juliane Hardt ◽  
...  
Keyword(s):  
Wound Healing ◽  
Moyamoya Disease ◽  
Clinical Symptoms ◽  
Healing Process ◽  
Superficial Temporal Artery ◽  
Surgical Techniques ◽  
Temporal Artery ◽  
Wound Complications ◽  
Wound Healing Process ◽  
Moyamoya Syndrome

OBJECTIVEMoyamoya vasculopathy (MMV) is a steno-occlusive cerebrovascular disease that can be treated by a surgical revascularization. All the revascularization techniques influence the blood supply of the scalp, with a risk for wound healing disorders. The authors’ aim was to analyze the wound healing process in the patients who underwent a direct or combined bypass surgery with a focus on different skin incisions.METHODSThe authors retrospectively identified all the patients with MMV who were treated surgically in their institution. Subsequently, they analyzed demographic data, clinical symptoms, surgical treatment, and detailed history of complications. Based on the evolution of their surgical techniques and the revascularization strategy to be used, the authors applied the following skin incisions: linear incision, curved incision, incomplete Y incision, and complete Y incision. Group comparisons regarding wound healing disorders were performed with significance testing using Fisher’s exact test.RESULTSThe authors identified 172 patients with MMV (61.6% moyamoya disease, 7% unilateral moyamoya disease, 29.7% moyamoya syndrome, and 1.7% unilateral moyamoya syndrome), of whom 124 underwent bilateral operations. One-quarter of the patients were juveniles. A total of 236 hemispheres were included in the analysis, of which 27.9% were treated by a combined procedure with encephalomyosynangiosis. Overall, 5.1% major and 1.7% minor wound complications occurred. The overall wound complication rate was lower in direct revascularization compared to combined revascularization (3% vs 15.2%). The lowest incidence of wound healing disorders was found in the linear incision group for the parietal superficial temporal artery branch (1.6%), followed by the incomplete Y incision group for the frontal branch of the superficial temporal artery (3.8%) in the direct bypass group. In the combined revascularization cohort, major or minor wound disorders appeared in 14.3% and 4.8%, respectively, in the complete Y incision group and in 4.2% (for both major and minor) in the curved incision group. The complete Y incision caused significantly more wound healing disorders compared to the remaining incision types (17.1% vs 3.1%, p = 0.007).CONCLUSIONSWound healing disorders are one of the major complications of revascularization surgery. Their incidence depends on the revascularization strategy and skin incision applied, with a complete Y incision giving the worst results.

scholarly journals Autologous micrograft accelerates endogenous wound healing response through ERK-induced cell migration

2019 ◽  
Author(s):  
Martina Balli ◽  
Francesca Vitali ◽  
Adrian Janiszewski ◽  
Ellen Caluwé ◽  
Alvaro Cortés-Calabuig ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
Skin Lesions ◽  
Wound Healing Process ◽  
Primary Fibroblast ◽  
Fibroblast Migration ◽  
Healing Response ◽  
Wound Healing Response

AbstractDefective fibroblast migration causes delayed wound healing (WH) and chronic skin lesions. Autologous micrograft (AMG) therapies have recently emerged as a new effective treatment able to improve wound healing capacity. However, the molecular mechanisms connecting their beneficial outcomes with the wound healing process are still unrevealed. Here, we show that AMG modulates primary fibroblast migration and accelerates skin re-epithelialization without affecting cell proliferation. We demonstrate that AMG is enriched in a pool of WH-associated growth factors that may provide the initiation signal for a faster endogenous wound healing response. This, in turn leads to increased cell migration rate by elevating activity of extracellular signal-regulated kinase (ERK) pathway and subsequent activation of matrix metalloproteinase expression and their extracellular enzymatic activity. Moreover, AMG-treated wounds showed increased granulation tissue formation and organized collagen content. Overall, we shed light on AMG molecular mechanism supporting its potential to trigger a highly improved wound healing process.

Use of Multilayer Thin-Film Coatings for Complete Restoration of Integument in Its Deep Damage (Experimental Study)

2012 ◽  
Vol 19 (2) ◽  
pp. 65-70
Author(s):  
I. E Nikityuk ◽  
V. V Petrash ◽  
V. A Kubasov
Keyword(s):  
Thin Film ◽  
Normal Skin ◽  
Healing Process ◽  
Clinical Signs ◽  
Control Group ◽  
Wound Healing Process ◽  
Multilayer Coatings ◽  
Multilayer Thin Film ◽  
Skin Damage ◽  
Complete Restoration

The effect of multilayer thin-film wound coatings upon regeneration of skin in its deep damage was studied 18 Chinchilla rabbits. Full-layer skin wounds 40 x 40 mm were inflicted on the back of the animals. In experimental group of animals the wounds were closed by multilayer coatings consisting of 50 aluminum metalized lavsan films. In control group one layer coating with thin aluminum plate was used. In all control animals the pyonecrotic wound processes developed within 1 — 2 weeks after intervention. In experimental rabbits the wound healing process showed no clinical signs of inflammation. In 6 weeks a full value regenerate in the zone of deep skin damage was formed. Its thickness and structure was typical of normal skin. Principle of multilayer coatings is perspective for the elaboration of coatings at treatment of deep and small wounds of various etiology when high quality of dermal regenerate is required.

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