Therapeutic Efficacy of Artificial Skin Produced by 3D Bioprinting

The skin protects the body from external barriers. Certain limitations exist in the development of technologies to rapidly prepare skin substitutes that are therapeutically effective in surgeries involving extensive burns and skin transplantation. Herein, we fabricated a structure similar to the skin layer by using skin-derived decellularized extracellular matrix (dECM) with bioink, keratinocytes, and fibroblasts using 3D-printing technology. The therapeutic effects of the produced skin were analyzed using a chimney model that mimicked the human wound-healing process. The 3D-printed skin substitutes exhibited rapid re-epithelialization and superior tissue regeneration effects compared to the control group. These results are expected to aid the development of technologies that can provide customized skin-replacement tissues produced easily and quickly via 3D-printing technology to patients.

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3D Printing and NIR Fluorescence Imaging Techniques for the Fabrication of Implants

Materials ◽

10.3390/ma13214819 ◽

2020 ◽

Vol 13 (21) ◽

pp. 4819

Author(s):

Yong Joon Suh ◽

Tae Hyeon Lim ◽

Hak Soo Choi ◽

Moon Suk Kim ◽

Sang Jin Lee ◽

...

Keyword(s):

3D Printing ◽

Fluorescence Imaging ◽

Near Infrared ◽

Imaging Techniques ◽

The Body ◽

Monitoring Method ◽

Printing Technology ◽

3D Printing Technology ◽

Nir Fluorescence ◽

Printing Techniques

Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for education, training, consultation, organ transplantation, plastic surgery, surgical planning, dentures, and more. In addition, the universal utilization of 3D printing techniques enables researchers to exploit different types of hardware and software in, for example, the surgical fields. To realize the 3D-printed structures to implant them in the body and tissue regeneration, it is important to understand 3D printing technology and its enabling technologies. This paper concisely reviews 3D printing techniques in terms of hardware, software, and materials with a focus on surgery. In addition, it reviews bioprinting technology and a non-invasive monitoring method using near-infrared (NIR) fluorescence, with special attention to the 3D-bioprinted tissue constructs. NIR fluorescence imaging applied to 3D printing technology can play a significant role in monitoring the therapeutic efficacy of 3D structures for clinical implants. Consequently, these techniques can provide individually customized products and improve the treatment outcome of surgeries.

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FUNCTION AND SAFETY EVALUATION OF 3D TECHNOLOGY TO PREPARE BONE REPAIR BIOMATERIALS

XXVI International Scientific and Practical Conference International Trends in Science and Technology ◽

10.31435/rsglobal_conf/28022021/7433 ◽

2021 ◽

Author(s):

Huseyn Elcin

Keyword(s):

Tensile Strength ◽

Composite Material ◽

3D Printing ◽

Biological Evaluation ◽

The Body ◽

3D Scaffold ◽

Printing Technology ◽

3D Printing Technology ◽

Proliferation And Differentiation

PLGA/HA composite biomaterials are prepared, and 3D printing technology is used to make bone scaffolds that can be implanted in the body. Its performance is tested by in vitro physical and biological methods, and its safety is evaluated by animal experiments. Methods: 3D printing technology was used to print the PLGA/HA composite three-dimensional stent biomaterial, and the tensile strength and bending strength of the stent material were tested with reference to GB/T1040 and GB/T9341 to verify its ability to support the proliferation and differentiation of hMSC. The biological evaluation standard (GB/T16886) evaluates the biocompatibility and biosafety of scaffoldmaterials in vitro and in vivo. Results: The porous 3D scaffold made of PLGA/HA composite material was successfully fabricated; the mechanical tensile strength and flexuralstrength of the composite material were 38 MPa and 42 MPa respectively, which were5.35 times and 5.25 times that of normal human cartilage; in vitro cell test It is proved that the 3D scaffold can support the proliferation and differentiation of hMSC into chondrocytes. The results of the biosafety test show that the scaffold meets the national medical device biological evaluation standards.

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Significance of integral hematological indexes for predicting postoperative wound healing in the experiment

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2018.03.49-54 ◽

2018 ◽

pp. 49-54

Author(s):

А.А. Супильников ◽

В.Н. Шабалин

Keyword(s):

Wound Healing ◽

Healing Process ◽

The Body ◽

Male Rats ◽

Control Group ◽

Wound Healing Process ◽

The Third ◽

Group A ◽

Clinically Significant ◽

White Mongrel

Цель исследования - оценка возможности использования интегральных гематологических индексов для выбора оптимальной модели ушивания послеоперационных ран в эксперименте. Методика. Исследование проводили на 297 белых беспородных крысах-самцах массой 190-210 г, которые были разделены на 3 группы, по 99 крыс в каждой. Одна группа являлась контрольной, у второй группы крыс был смоделирован раневой процесс с натяжением брюшной стенки; у третьей группы животных - модель раневого процесса с применением трансплантата нанокомпозитного материала. Определяли следующие интегральные гематологические индексы: индекс Кребса; коэффициент Бредекка; лейкоцитарный индекс; индекс соотношения нейтрофилов и моноцитов; индекс соотношения лимфоцитов и моноцитов; индекс соотношения лимфоцитов и эозинофилов; коэффициент отношения альбуминов к глобулинам; оксипролиновый коэффициент. Результаты. Установлены статистически значимые колебания показателей интегральных гематологических индексов в динамике заживления послеоперационной раны у крыс. Заключение. На основе полученных результатов был сделан вывод, что особенности динамики интегральных гематологических индексов, выявленные на различных моделях раневого процесса, отражают неспецифические и специфические защитные реакции организма в целом, позволяют делать прогноз дальнейшего течения и исхода раневого процесса, а также обеспечивают возможность выбора оптимальной модели ушивания послеоперационной раны. The article presents results of predicting the wound healing process using integral hematological indexes. The aim of the study was to describe the dynamics of integral hematological indexes of wound healing process in rats to predict its outcome. The study included 297 white mongrel male rats weighing 190-210 g, which were divided into three groups, 99 rats in each. One group was a control group; in the second group, a wound healing process with abdominal wall tension was modeled; in the third group, a wound healing process with a graft was modeled. The following integrated hematological indexes were determined: Krebs index; Bredeck index; leukocyte index; neutrophil/monocyte ratio; lymphocyte/monocyte ratio; lymphocyte/eosinophil ratio; albumin/globulin ratio; and oxyproline coefficient. The study showed that changes in values of integral hematological indexes were mostly statistically and clinically significant. We concluded that changes in integral hematological indexes in different models of wound healing reflect nonspecific and specific protective responses of the body as a whole and allow to predict further course and outcome of the wound healing process.

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Endoscopic Treatment of Symptomatic Foot and Ankle Bone Cyst with 3D Printing Application

BioMed Research International ◽

10.1155/2020/8323658 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Changgui Zhang ◽

Jin Cao ◽

Hongli Zhu ◽

Huaquan Fan ◽

Liu Yang ◽

...

Keyword(s):

3D Printing ◽

Control Group ◽

Bone Cysts ◽

Foot And Ankle ◽

Printing Technology ◽

3D Printing Technology ◽

Intraoperative Fluoroscopy ◽

The Times ◽

Experimental Group

Objective. To study the efficacy of arthroscopy for treating symptomatic bone cysts of the foot and ankle through the follow-up of patients and to further explore the application value of 3D printing technology in this treatment. Methods. Twenty-one patients with symptomatic bone cysts in the foot and ankle who underwent arthroscopic surgery in our Center from March 2010 to December 2018 were enrolled, including 11 in the experimental group and 10 in the control group. For the control group, C-arm fluoroscopy was used intraoperatively to confirm the positioning of the cysts; for the experimental group, a 3D model of the lesion tissue and the 3D-printed individualized guides were prepared to assist the positioning of the cysts. Debridement of the lesion tissues was conducted under an arthroscope. Regular follow-ups were conducted. The time of establishing arthroscopic approaches and the times of intraoperative fluoroscopy between the two groups were compared. Significance was determined as P < 0.05 . Results. The postoperative pathology of the patients confirmed the diagnosis. No significant perioperative complications were observed in either group, and no recurrence of bone cysts was seen at the last follow-up. The VAS scores and AOFAS scores of the two groups at the last follow-up were significantly improved compared with the preoperative data, but there was no statistical difference between the two groups. All surgeries were performed by the same senior surgeon. The time taken to establish the arthroscopic approaches between the two groups was statistically significant ( P < 0.001 ), and the times of intraoperative fluoroscopy required to establish the approach were also statistically significant ( P < 0.001 ). The intraoperative bleeding between the two groups was statistically significant ( P < 0.01 ). There was 1 case in each group whose postoperative CT showed insufficient bone grafting, but no increase in cavity volume was observed during the follow-up. Conclusion. With the assistance of the 3D printing technology for treating symptomatic bone cysts of the ankle and foot, the surgeon can design the operation preoperatively and perform the rehearsal, which would make it easier to establish the arthroscopic approach, better understand the anatomy, and make the operation smoother. This trial is registered with http://www.clinicaltrials.govNCT03152916.

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Evaluation of the clinical efficacy of 3D Printing technology assisted surgery combined with early postoperative comprehensive rehabilitation in the treatment of Senile Intertrochanteric Fractures

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.37.3.3988 ◽

2021 ◽

Vol 37 (3) ◽

Author(s):

Ye Zhao ◽

Yong-hui Liu ◽

Shu-ge Mao ◽

Xin-xin Zhang

Keyword(s):

3D Printing ◽

Fracture Healing ◽

Hip Joint ◽

Weight Bearing ◽

Control Group ◽

Intertrochanteric Fractures ◽

Printing Technology ◽

3D Printing Technology ◽

Comprehensive Rehabilitation ◽

Experimental Group

Objectives: To observe the clinical value of 3D printing technology assisted surgery combined with early postoperative comprehensive rehabilitation in elderly patients with intertrochanteric fractures. Methods: Sixty elderly patients with intertrochanteric fractures of the femur who were treated in our hospital from January 2018 to January 2020 were selected and randomly divided into two groups. In the experimental group, 3D printing technology assisted surgery combined with early postoperative comprehensive rehabilitation was used for treatment. While in the control group, traditional open reduction and dynamic hip screw internal fixation combined with postoperative conventional treatment was utilized. The duration of surgery, intraoperative blood loss, postoperative hospital stay, weight bearing time, fracture healing time and other surgical indicators were recorded respectively, and hip joint function recovery was evaluated prior to and 2 weeks after surgery. All patients were followed up for six months to observe the occurrence of complications within half a year, including deep vein thrombosis, incision infection, avascular necrosis of femoral head, hip joint stiffness, delayed fracture healing, etc. Subsequently, the differences in postoperative complications between the two groups were compared and analyzed. Results: The operation time, blood loss, postoperative hospital stay, weight bearing time and fracture healing time of the experimental group were better than those of the control group, and the difference was statistically significant (p<0.05). After treatment, the hip joint function of the experimental group was significantly improved compared with the control group, with a statistically significant difference(p=0.03). The incidence of operative complications in the experimental group was 10% (3/30) within six months postoperatively, significantly lower than the 33% (10/30) in the control group, with statistical significance (p=0.03). Conclusion: 3D printing with early rehab proved to be effective treatment in our study. Such a combined treatment has the advantages of precise operative reduction, fast postoperative recovery, and certain safety and effectiveness. doi: https://doi.org/10.12669/pjms.37.3.3988 How to cite this:Zhao Y, Liu YH, Mao SG, Zhang XX. Evaluation of the clinical efficacy of 3D Printing technology assisted surgery combined with early postoperative comprehensive rehabilitation in the treatment of Senile Intertrochanteric Fractures. Pak J Med Sci. 2021;37(3):---------. doi: https://doi.org/10.12669/pjms.37.3.3988 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Interventional Therapy for Cerebral Aneurysms Under the Guidance of 3D Printing Technology

Proceedings of Anticancer Research ◽

10.26689/par.v4i4.1406 ◽

2020 ◽

Vol 4 (4) ◽

Author(s):

Xiangkong Song ◽

Xinguo Sun ◽

Hualong Wang ◽

Jie Qi ◽

Guoqing Wang

Keyword(s):

3D Printing ◽

Research Work ◽

Cerebral Aneurysms ◽

Length Of Hospital Stay ◽

Interventional Therapy ◽

Control Group ◽

Printing Technology ◽

Clinical Method ◽

3D Printing Technology ◽

Experimental Group

Objective: To explore the clinical method and effect of 3D printing in the treatment of cerebral aneurysms. Methods: The authors research work on the hospital, work time in February 2019 - February 2020, this study selected patients of cerebral aneurysms, this period are selected for treatment of 100 cases of patients, randomly divided into two groups, a group to give simple intervention, named as the control group, another group for the interventional therapy under the guidance of 3 D printing, named as experimental group, analyze the effect of two groups of patients with clinical intervention. Results: The length of hospital stay in the experimental group was shorter than that in the control group. Meanwhile, the incidence of complications and adverse reactions in the experimental group and the control group were 6.00% and 18.00%, the experimental group was better(P<0.05). Conclusion: 3D printing technology can be applied in the treatment of patients with cerebral aneurysms to provide guidance for interventional surgical treatment. It has significant effect, can reduce the incidence of complications in patients, has significant clinical effect, and can be popularized.

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A 3D Printing Personalized Percutaneous Nephroscope Puncture Guide Plate in Percutaneous Nephrolithotomy: A Pilot Study

10.21203/rs.3.rs-724657/v1 ◽

2021 ◽

Author(s):

Gao Keyu ◽

Li Shuaishuai ◽

Ashok raj ◽

Li Shuofeng ◽

Liu Shuai ◽

...

Keyword(s):

3D Printing ◽

Blood Loss ◽

Percutaneous Nephrolithotomy ◽

Color Doppler ◽

Control Group ◽

Intraoperative Blood Loss ◽

Printing Technology ◽

3D Printing Technology ◽

Guide Plate ◽

Significant Difference

Abstract Objective Using CTU, combined with 3D printing technology, digital design, the development of individualized PCNL) puncture guides, preliminary discussion of its feasibility for PCNL puncture positioning.Methods Twenty-two patients with renal calculi who underwent PCNL in the hospital of Xuzhou Medical University in 2017-2018, including 10 experimental groups, used 3D printing technology to make guides (puncture guides); simulated punctures in vitro, after clearing the needle angle, PCNL was performed in 10 patients and PCNL in 12 patients in the control group. The accuracy of puncture positioning, puncture time and intraoperative blood loss were compared between the two groups.Results In the experimental group, 10 patients had good puncture guide plate and the patient's skin. The puncture needle was puncture under the guidance of the guide plate and verified by the color Doppler. The 1 needle puncture was successful 100.00% (10/10), the positioning of the needle point, the depth of the puncture. The angles were consistent with the preoperative design. The success rate of 12 cases of color Doppler ultrasound in the control group was 75.00% (9/12). There was no significant difference between the two groups (P>0.05). The amount of hemorrhage was (7.78±0.94) min and (49.31±6.43) mL, respectively. The control group was (9.04±1.09) min and (60.08±12.18) mL, respectively. The two groups were statistically significant (P<0.05). Conclusion 3D printing personalized percutaneous nephrolithotomy guide can improve the accuracy of PCNL renal puncture channel positioning, shorten the puncture time and reduce intraoperative blood loss, and provide a new method for PCNL renal puncture positioning, which is worthy of further clinical exploration.

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3D PRINTING TECHNOLOGY IN TEXTILE AND FASHION INDUSTRY

Fashion Industry ◽

10.30857/2706-5898.2020.3.2 ◽

2021 ◽

pp. 41-44

Author(s):

Diana-Roxana Viziteu ◽

Antonela Curteza

Keyword(s):

3D Printing ◽

Industrial Revolution ◽

New Technologies ◽

Thermoplastic Polyurethane ◽

3D Modelling ◽

The Body ◽

Protective Equipment ◽

Fashion Industry ◽

Printing Technology ◽

3D Printing Technology

The extraordinary thing about the application of 3D printing technology is that it can be used to create accessible items customized to personal needs. In the fashion industry, there is a need for individualized protective equipment. The possibility of applying new technologies such as 3D modelling of protective elements that can be made by using 3D printers is presented in this paper. 3D modelling and additive technologies (3D printing) can be used in the development of protective work clothing. The fabrication process only requires the digital fi le with the 3D model and the right material - we chose to use thermoplastic polyurethane (TPU).The design samples were constructed and modelled using a software program called Rhinoceros. The samples can be integrated into the clothing item, in order to follow the body shape and to provide the necessary protection. Purpose. This paper aims to explore the applicability of 3D printing materials using thermoplastic polyurethane (TPU) for the development of protective gear. Scientifi c novelty. In the fashion industry, three-dimensional (3D) printing has been used by designers and engineers to create everything from accessories to clothing, but only a few studies have investigated its applicability in personal protective equipment. Practical value. One of the most signifi cant technologies of the fourth industrial revolution is 3D printing. Additive manufacturing and 3D printing are the subject of intensive research and development (methods, materials, new techniques, application areas, etc.). The purpose of this study is to develop 3D printing samples and study conditions related to TPU.

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PERBEDAAN EFEKTIFITAS PEMBERIAN TOPIKAL GEL Spirulina platenis EKSTRAK ETANOL 96% DAN 70% TERHADAP KEPADATAN SERABUT KOLAGEN PADA PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽

10.30649/denta.v13i1.180 ◽

2019 ◽

Vol 13 (1) ◽

Author(s):

Nafiah Nafiah ◽

Sarianoferni Sarianoferni ◽

Rizqi Ananda Putri

Keyword(s):

Spirulina Platensis ◽

Healing Process ◽

Ethanol Extract ◽

Topical Administration ◽

The Body ◽

Control Group ◽

Wound Healing Process ◽

Group Score ◽

Alternative Ingredients ◽

Significant Difference

Background: Pravalence of Traumatic Ulcers is quite high in Indonesia. Therapy that has been carried out is still symptomatic, now there are many herbal ingredients that can be used as an alternative ingredients for medicine, including spirulina platensis. Spirulina platensis is one type of biological wealth that is widespread in Indonesian marine waters, and has bioactive ingredients that are beneficial to the body and can help the wound healing process. Purpose: to determine the differences in the effectiveness of topical administration of spirulina platensis ethanol extract 96% and 70% against the density of collagen fibers in healing traumatic ulcers. Methods: The subjects of the study were 36 wistar rattus novergicus rats divided into 6 groups, treated with traumatic ulcers in the lower labial mucosa, treated according to their respective groups K1.3 (control group 3 days), P1.3 (Giving spirulina gel platensis ethanol extract 96% 3 days), P2.3 (Giving spirulina platensis ethanol extract 70% 3 days), K1.7 (Control group 7 days), P1.7 (Giving spirulina platensis ethanol extract 96% ethanol 7 days ), P2.7 Giving spirulina platensis ethanol extract 70% 7 days 7). Results: There were differences in the effectiveness of giving spirulina platensis ethanol extract 96% and 70%. There is a significant difference in the P1.7 group (score = 4) with P2.7 (score = 3), P1.3 (score = 3) with P1.7 (4), P1.3 (score = 3) with P2 .3 (score 3), K1.3 (score = 2) with P1.3 (score = 3) and P2.3 (score = 3), K1.7 (score = 3) with P1.7 (score = 4) ) and P2.7 (3). Conclusion: There was no difference in effectiveness between 96% and 70% spirulina platensis ethanol extract gel on day 3 and there was a difference in effectiveness between 96% spirulina platensis ethanol extract gel on day 7.

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Uji Efektivitas Ekstrak Serai (Cymbopogon citratus) terhadap Penyembuhan Luka Sayat pada Mencit Putih

Jurnal Surya Medika ◽

10.33084/jsm.v5i1.942 ◽

2019 ◽

Vol 5 (1) ◽

pp. 13-21

Author(s):

Maharuni Nurqadriasti Djuddawi ◽

Haryati Haryati ◽

Andi Noor Kholidha

Keyword(s):

Wound Healing ◽

Healing Process ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

Homogeneity Test ◽

Cymbopogon Citratus

The wound is an injury to the part of the body where the skin and underlying tissues lose its tissue continuity. Lemongrass is one of the grass plants that often used as a medicine that can function as an antibacterial, anti-inflammatory, and antioxidant that can affect the wound healing process. This study aims to evaluate the effectiveness of lemongrass extract (Cymbopogon citratus) on wound healing in white mice. This research is true experimental research with a post-test only control group design. This research used 25 white mice divided into 5 groups: 30% lemongrass extract, 60% lemongrass extract, 80% lemongrass extract, negative control (Aquades) and positive control (Bioplacenton). The grouping of subjects was carried out randomly. Normality test using the Shapiro Wilk test shows the p-value in each group > 0.05, it can be concluded that the data distribution is normal. Levene's Test homogeneity test was obtained p-value = 0.730 (p> 0.05), thus the data variant is homogeneous. The results of the analysis with One-Way ANOVA test showed p-value = 0.881 (p > 0.05). There was no difference in the effectiveness of lemongrass extract (Cymbopogon citratus) 30%, 60%, and 80% compared to negative control groups and positive control of wound healing in white mice. However, wound healing in the 60% lemongrass extract group was faster and total wound healing had occurred on the 14th day. Wound healing is slowest in the 80% lemongrass extract group.

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