scholarly journals THE EFFECTS OF GENISTEIN-RICH EDAMAME ON LDL, HS-CRP LEVEL, NEUTROPHIL-LYMPHOCYTE RATIO AND MONOCYTE-LYMPHOCYTE RATIO: STUDY ON RAT MODEL OF ATHEROSCLEROSIS: Experimental Study in Atherosclerosis Induced Male Rats

2021 ◽  
Vol 8 (1) ◽  
pp. 39-47
Author(s):  
Reza Dian Pratama ◽  
Nani Maharani ◽  
Udin Bahrudin
Keyword(s):  
Experimental Study ◽  
Intervention Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Significant Difference ◽  
Hs Crp

ABSTRACT Background: Atherosclerosis is related to hypercholesterolaemia and inflammation. Edamame is one of the soybeans with high levels of genistein which protects the formation of atherosclerosis. Purpose: This research aimed to evaluate the effects of genistein-rich edamame on LDL, hs-CRP, Neutrophil-Lymphocyte Ratio (NLR) and Monocyte-Lymphocyte Ratio (MLR) on atherosclerosis-induced mice.   Method: This research is a true experimental study with posttest only-controlled group design. Thirty male rats were divided randomly into five groups: negative control group without any intervention, positive control group with atherosclerosis induction, first intervention group with edamame extract supplementation, second intervention group with genistein-rich edamame extract supplementation, and third intervention group treated with atorvastatin. After 28 days, serum LDL, hs-CRP, LDL and MLR were measured.   Results: There were significant differences (p<0.001) of hs-CRP values between the second intervention group compared to the negative control, positive control and first intervention group. Edamame extract lowered LDL cholesterol levels in the second intervention group compared to positive control, but the difference were not statistically significant. There were significant differences (p<0.05) of MLR values between the second intervention group and the negative control and also the first intervention group, and significant differences between the second intervention group and the negative control and also the first intervention group. There was no significant difference on NLR values between all groups.   Conclusion: The administration of genistein rich edamame may reduces the inflammation in the blood vessels better than edamame extract represented by lower hs-CRP and MLR values compared to the edamame extract.   Keywords: Genistein rich-edamame, LDL, hsCRP, neutrophil-lymphocyte ratio, monocyte-lymphocyte ratio

Comparison of hematological parameters and perinatal outcomes between COVID-19 pregnancies and healthy pregnancy cohort

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Esin Merve Erol Koç ◽  
Rahime Bedir Fındık ◽  
Hatice Akkaya ◽  
Işılay Karadağ ◽  
Eda Özden Tokalıoğlu ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Perinatal Outcomes ◽  
Control Group ◽  
Strong Positive Correlation ◽  
Monocyte Count ◽  
Distribution Width ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Significant Difference ◽  
Adverse Perinatal Outcomes

AbstractObjectivesTo evaluate the relationship between Coronavirus Disease 2019 (COVID-19) in pregnancy and adverse perinatal outcomes. The secondary aim is to analyze the diagnostic value of hematologic parameters in COVID-19 complicated pregnancies.MethodsThe current study is conducted in a high volume tertiary obstetrics center burdened by COVID-19 pandemics, in Turkey. In this cohort study, perinatal outcomes and complete blood count indices performed at the time of admission of 39 pregnancies (Study group) complicated by COVID-19 were compared with 69 uncomplicated pregnancies (Control group).ResultsThere was no significant difference between the obstetric and neonatal outcomes of pregnancies with COVID-19 compared to data of healthy pregnancies, except the increased C-section rate (p=0.026). Monocyte count, red cell distribution width (RDW), neutrophil/lymphocyte ratio (NLR), and monocyte/lymphocyte ratio (MLR) were significantly increased (p<0.0001, p=0.009, p=0.043, p<0.0001, respectively) whereas the MPV and plateletcrit were significantly decreased (p=0.001, p=0.008) in pregnants with COVID-19. ROC analysis revealed that the optimal cut-off value for MLR was 0.354 which indicated 96.7% specificity and 59.5% sensitivity in diagnosis of pregnant women with COVID-19. A strong positive correlation was found between the MLR and the presence of cough symptom (r=41.4, p=<0.0001).ConclusionsThe study revealed that, pregnancies complicated by COVID-19 is not related with adverse perinatal outcomes. MLR may serve as a supportive diagnostic parameter together with the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) in assessment of COVID-19 in pregnant cohort.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Katarzyna Olczak ◽  
Halina Pawlicka
Keyword(s):  
Continuous Wave ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Thermal Methods ◽  
Root Canals ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

scholarly journals Assessment of the hemogram parameters in patients with paroxysmal supraventricular tachycardia: a retrospective study

2020 ◽  
Vol 66 (10) ◽  
pp. 1371-1375
Author(s):  
Mehmet Cosgun ◽  
Yilmaz Gunes ◽  
Isa Sincer ◽  
Asli Kurtar Mansiroglu
Keyword(s):  
Retrospective Study ◽  
Supraventricular Tachycardia ◽  
University Hospital ◽  
Control Group ◽  
Paroxysmal Supraventricular Tachycardia ◽  
Monocyte Count ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Markers Of Inflammation ◽  
Significant Difference

SUMMARY OBJECTIVE: Inflammation has been suggested as a potential mechanism in the pathogenesis of arrhythmia. Hemogram parameters such as monocyte count to high-density lipoprotein cholesterol ratio (MHR), neutrophil/lymphocyte ratio (NLR), and monocyte/lymphocyte ratio (MLR) have been considered to be markers of inflammation and new cardiovascular risk predictors. This retrospective study aimed to investigate the relationship between MHR, NLR, and MLR in patients with paroxysmal supraventricular tachycardia (PSVT). METHODS: A retrospective study conducted at a university hospital in Bolu, Turkey, between 2017 and 2019. Our study included 196 patients who underwent electrophysiological study (EPS) due to palpitation or documented PSVT on electrocardiography (ECG). Patients having documented atrioventricular nodal re-entrant tachycardia (AVNRT) on ECG or inducible AVNRT on EPS were included in the PSVT group (n=130), and patients with palpitation but without inducible arrhythmia on EPS (n=66) were included in the control group. Routine biochemical and hemogram tests were performed before the EPS procedure. RESULTS: When hemogram parameters were compared, there was no statistically significant difference in MHR values [0.010 (0.001-0.030) vs 0.010 (0.001-0.020) p =0.67]. Additionally, both NLR [2.21(0.74-11.36) vs 1.98(0.72-24.87) p=0.13] and MLR [0.25 (0.03-1.05) vs 0.24(0.07-1.39) p=0.41] were not statistically significant between the two groups. CONCLUSION: There is no significant difference in PSVT patients regarding hemogram parameters including white blood cell subtypes, MLR, NLR, and MHR. Therefore the evaluation of hemogram parameters may not be clinically relevant for PSVT patients.

scholarly journals A Digital Microscopic Inspection of Dentinal Defects after Using Endodontic Retreatment Files

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Ammar AbuMostafa ◽  
Hala Almoqayyad ◽  
Al-Omari Mohammad
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Material ◽  
Root Canals ◽  
Root Canal Retreatment ◽  
Significant Difference ◽  
Digital Microscope ◽  
Microscopic Inspection

Aim. The study aimed at evaluating the incidence of dentinal defects after root canal retreatment with ProTaper Universal retreatment (PTUR) and XP-endo Shaper and Finisher R (XP). Materials and Methods. Sixty extracted single-rooted human premolars were selected and divided into 4 groups of 15 teeth each. In the negative control group, the teeth were left unprepared. In the positive control group, the teeth were prepared with ProTaper Next and obturated with no further retreatment. In the PTUR and XP groups, the teeth were prepared and obturated followed by removal of the filling material at body temperature using PTUR and XP instruments, respectively. The roots were then sectioned at 3, 6, and 9 mm from the apex and observed under a digital microscope to detect defects. Results. PTUR group showed significantly higher ( p value <0.05) incidence of defects than the other groups. Comparison of no defects versus defects between groups in different areas of root canals demonstrated significant difference among the groups in the apical and cervical regions. Conclusion. Within the limitations of the present study, PTUR files created significantly more dentinal defects than XP files, with most of those defects at the cervical and apical areas of the root canals.

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals Preliminary Results on the Effectiveness of a Nutrition Intervention in Lowering Diabetes Risk in Prediabetic People Living with HIV (PLWH) in MASH Cohort (OR36-06-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Alicia Sneij Perez ◽  
Adriana Campa ◽  
Leslie Seminario ◽  
Sabrina Martinez ◽  
Fatma Huffman ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Intervention Group ◽  
Nutrition Intervention ◽  
Control Group ◽  
Reactive Protein ◽  
Research Initiative ◽  
Significant Difference ◽  
Hs Crp

Abstract Objectives The objective of this study is to assess the effectiveness of a 6-month nutrition intervention to improve glycemic parameters and inflammation in prediabetic PLWH on stable ART with undetectable HIV viral load. Methods A 6-month randomized, controlled nutrition intervention was conducted in prediabetic PLWH. The study participants for the intervention were recruited from the Miami Adult Studies for HIV (MASH) cohort at the FIU-Borinquen Research Clinic. Upon their consent, the participants were randomized into the intervention group or the control group. Participants randomized in the intervention group met once a month for approximately 1 hour where they received medical nutrition therapy, nutrition counseling and nutrition education; participants randomized into the control group received educational material at baseline. Blood was drawn at baseline and at 6-month to measure fasting blood glucose (FBG) and high sensitivity C-reactive protein (hs-CRP). Results A total of 38 participants were recruited and randomized into either the intervention group (n = 20) or the control group (n = 18). We found that the FBG for the 6-month follow-up for the intervention group was significantly lower than the baseline FBG values of the same study group (paired t-test; P = 0.031). No significant difference was found in the control group between the baseline and 6-month fasting blood glucose values (P = 0.068). Moreover, no significant difference was found in pre/post C-reactive protein (CRP) levels in the intervention or control group (paired t-test; P = 0.404 and P = 0.117 respectively). There was a significant difference in CRP levels at baseline (P = 0.028) between the study groups but no difference at the 6-month follow up (Mann Whitney U test: P = 0.430). Conclusions The results from this intervention support the notion that a nutrition intervention is effective in prediabetic PLWH to lower diabetes risk by significantly lowering fasting blood glucose and may be implemented into larger scale interventions; however, no significant changes was seen in hs-CRP values between the 2 groups. Funding Sources National Institute of General Medical Sciences (NIGMS): Research Initiative for Scientific Enhancement (RISE), Biomedical Research Initiative (BRI) Grant, National Institute on Drug Abuse 5U01DA040381-03 and FIU-Dissertation Funding.

scholarly journals BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 225
Author(s):  
Sulaeman A ◽  
Patonah Patonah ◽  
Patonah Patonah ◽  
Negara Gg ◽  
Negara Gg
Keyword(s):  
Test Group ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Adipose Tissues ◽  
Obese Male

  Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

scholarly journals MAT, a Novel Polyherbal Aphrodisiac Formulation, Enhances Sexual Function and Nrf2/HO-1 Pathway While Reducing Oxidative Damage in Male Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Kazim Sahin ◽  
Mehmet Tuzcu ◽  
Cemal Orhan ◽  
Hasan Gencoglu ◽  
Nurhan Sahin ◽  
...  
Keyword(s):  
Sexual Function ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Mucuna Pruriens ◽  
Tribulus Terrestris ◽  
Sexual Hormones ◽  
Significant Difference ◽  
Group 5

Mucuna pruriens, Ashwagandha, and Tribulus terrestris are known as the enhancers for sexual health, functional activities, vitality, and longevity. These herbs had been widely used in the Ayurveda medicine as aphrodisiacs through the ages, and their efficacy was also verified separately in our previous publication. Therefore, the aim of this study was to determine the effects of Mucuna, Ashwagandha, and Tribulus complexes on sexual function in rats. Twenty-eight male rats allocated to four groups as follows: (i) negative control (C); (ii) positive control or sildenafil citrate treated group (5 mg/kg) (S); (iii) MAT1 (combination of 10 mg Mucuna (M) + 10 mg Ashwagandha (A) + 10 mg Tribulus (T)/kg BW); (iv) MAT 2 (20 mg Mucuna + 20 mg Ashwagandha + 20 mg Tribulus/kg BW). There was no significant difference found between the MAT1 and MAT2 groups while they showed significantly increased testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels when compared to the negative control. Significant increases in Nrf2/HO1 levels and decreases in NF-κB were detected in MAT groups similar to the decrease in serum and testis malondialdehyde (MDA) levels as compared to both controls. The sperm motility, count, and rate also significantly improved in both MAT groups, while ALT, AST, creatinine, ALP, and urea levels did not change in any of the groups. Oral consumption of MATs combination in male rats resulted in inhibition of NF-κB and MDA and also increased sex hormones with Nrf2-mediated HO-1 induction. MAT combinations may improve sexual functions by increasing levels of sexual hormones and regulation of NF-κB and Nrf2/HO-1 signaling pathways.

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