arteriosclerosis obliterans
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2021 ◽  
Vol 5 (5) ◽  
pp. 108-112
Author(s):  
Linbo Liu ◽  
Yang Liu ◽  
Hongjun Wang ◽  
Yi Zhang ◽  
Zhijie Liao ◽  
...  

At present, the incidence rate of arteriosclerosis obliterans (LEASO) of the lower extremities is significantly increased by aging and lifestyle changes. It is of great importance to predict the LEASO effectively and accurately by analyzing the imaging data of the lower extremities [1]. At this stage, China has entered the era of big data and artificial intelligence. Medical institutions at all levels can produce a large number of lower limb vascular image data every day. Using big data deep learning technology to intelligently analyze a large number of image data, and then carry out auxiliary diagnosis, so as to improve the diagnosis and treatment effect of LEASO is the focus of clinical research.


2021 ◽  
Vol 3 (3) ◽  
pp. 39-49
Author(s):  
Fuxu GE ◽  
Jingbao WANG ◽  
Laijie LIU ◽  
Wansheng LI ◽  
Bin ZHAO

Objective To explore the efficacy of wenmaitongguan decoction combined with Moxibustion in the treatment of lower extremity arteriosclerosis obliterans. Methods From January 2020 to January 2021, 150 patients with lower extremity arteriosclerosis obliterans were randomly divided into treatment group (76 cases) and control group (74 cases). The treatment group was treated with wenmaitongguan decoction combined with moxibustion, and the control group was treated with alprostadil. The clinical efficacy, the distance of intermittent claudication, brachial femoral index, no, endothelin-1 and inflammatory factor (TNF-α、CRP) were compared between the two groups before treatment, 7 days and 4 weeks after treatment。 Results 1. There was no difference in vascular endothelial function before treatment (P > 0.05); The level of ET-1 in the treatment group was significantly lower than that in the control group at 7 days and 4 weeks after treatment (P < 0.05), while the level of no in the treatment group was significantly higher than that in the control group (P < 0.05). 2. The level of ABI and intermittent claudication at 7 days and 4 weeks after treatment were significantly improved (P < 0.05), The difference was statistically significant (P < 0.05). 3. Tumor necrosis factor (TNF--α) in two groups、 Comparison of C-reactive protein (CRP),before treatment,There was no difference in CRP、TNF-α (P > 0.05); The level of water in the treatment group was significantly lower than that in the control group 7 days and 4 weeks after treatment (P < 0.05). Conclusion: wenmaitongguan decoction combined with moxibustion can alleviate clinical symptoms by reducing vascular endothelial injury, reducing vascular endothelial inflammatory reaction, restoring vascular tension, expanding peripheral blood vessels and improving peripheral microcirculation.


2021 ◽  
Vol 102 (4) ◽  
pp. 453-458
Author(s):  
R E Kalinin ◽  
I A Suchkov ◽  
E A Klimentova ◽  
A V Shchulkin ◽  
A A Gerasimov ◽  
...  

Aim. To assess the number of markers of apoptosis and cell proliferation, as well as their relationships in the area of restenosis of arterial reconstructions. Methods. The study included 14 patients with a diagnosis of arteriosclerosis obliterans of the lower extremities. Post-thrombotic occlusion of femoropopliteal bypass. All patients were males with stage III disease according to the Fontaine classification modified by A.V. Pokrovsky. The average age of the patients was 653.4 years. The mean disease duration was 92.5 months after the initial intervention. Intraoperative material distal anastomosis of femoropopliteal bypass was taken from patients during arterial reconstructions. As a control, we used arterial wall samples obtained at organ procurement from postmortem donors without arteriosclerosis obliterans of the lower extremities. The number of samples is 8. The site of their collection is the popliteal artery. After sampling, they were crushed, and a homogenate was prepared, followed by the determination of the amount of p53, PDGF BB, Bcl2, and Bax proteins using the enzyme immunoassay. Statistical analysis was performed using the Statistica 10.0 software. Group differences were assessed by using the MannWhitney test. Correlation coefficients were determined using the Spearman test. Data are presented as medians and interquartile ranges. Results. In tissue samples of restenosis, the amount of p53 protein was 0.07 units/mg and was significantly reduced compared with the control samples 0.14 units/mg (р=0,015). The amount of platelet-derived growth factor PDGF BB was 0.17 ng/mg (р=0.05), Bcl2 1.61 ng/mg (р=0.008), Bax 6.0 ng/mg (р=0.25) in the restenosis area and was increased in comparison with the control samples (0.04 ng/mg, 0.9 ng/mg, 4.4 ng/mg, respectively). A relationship between p53 and platelet-derived growth factor BB (r=0.724, p=0.002), platelet-derived growth factor BB and Bcl2 (r=0.672, p=0.003) was revealed in samples from restenosis tissue obtained during arterial reconstructions. Conclusion. The decreased apoptosis, expressed in a low level of p53 protein, with an increased Bax/Bcl-2 ratio is associated with an increase in the proliferative response of vascular wall cells in the area of restenosis of arterial reconstruction.


2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Ruhang Zhou ◽  
Hongyan Zhai ◽  
Zhiming Yin ◽  
Jian Cui ◽  
Nan Hu

This study was to evaluate the biomechanical characteristics of the vascular wall during virtual reality- (VR-) assisted percutaneous transluminal angioplasty (PTA) and its effect on the treatment of lower-extremity arteriosclerosis obliterans (LEAO). In this study, a three-dimensional (3D) model and a finite-element model of arteries were constructed first, and various fluid mechanics were analyzed. Then, the virtual expansion simulation (VES) of individualized PTA was performed based on the ABAQUS/Explicit module to analyze the interaction between the balloon and the blood vessel at different times and the changes in the vascular shape and structural stress distribution. Finally, an LEAO animal model was constructed. Based on conventional PTA (PTA group) and VR-assisted PTA (VR-PTA) treatment, the morphological changes of vascular lumen of the two animal models were evaluated. The results showed that the normal, stenotic blood vessels and blood models were successfully constructed; the pressure of the stenotic blood vessel at the stenosis decreased obviously and the shear stress of blood vessel wall increased compared with that of the normal blood vessels, and there may be a blood reflux area in the poststenosis stage. The simulation results of the VES showed that the maximum principal stress value at 3 mm of the marginal vessel was much lower than that at 5 mm (about 10% lower), so the maximum principal stress change within 2 mm of the balloon-expanded vessel was the most obvious. The treatment results of the animal model showed that the VR-PTA group showed an obvious increase in the diameter of the vascular lumen, a decrease in the intima and media area, and a decrease in the thickness of the vessel wall in contrast to the PTA group P < 0.05 , which had an important effect on the reconstruction and expansion of the vascular lumen. The VR-PTA treatment on LEAO was realized in this study, which provided critical reference for the follow-up application of VR technology in the evaluation of surgical plan and research on biomechanical mechanisms of restenosis after PTA.


Author(s):  
Songjie Hu ◽  
Zhongjian Xu ◽  
Dehai Lang

IntroductionAtherosclerosis is a continuously worsening chronic condition that starts in the arteries and may then affect other blood vessels. There is increasing evidence linking microRNAs to the development of arteriosclerosis obliterans (ASO). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was applied in this study to detect miR-210-5p (miR-210) expression, and significant upregulation was observed in human arterial walls where ASO was prevalent.Material and methodsThe proliferation of human umbilical vascular endothelial cells (HUVECs) with various levels of miR-210 expression was assessed via MTT and colony-formation assays. Cell proliferation was significantly promoted in HUVECs with upregulated miR-210 levels and reduced in HUVECs with downregulated miR-210 levels.ResultsFlow cytometric analysis of cells stained with annexin V-FITC and propidium iodide data demonstrated that miR-210 inhibited apoptosis, while miR-210 inhibition promoted apoptosis by mediating pro-apoptotic protein expression levels. These results were verified using a dual-luciferase reporter gene assay system, which showed that Janus kinase 1 (JAK1) was directly targeted by miR-210, while an miR-210 mimic significantly decreased downstream JAK1 and signal transducer and activator of transcription 3 (STAT3) activation at a post-transcriptional level in HUVECs, as detected by western blotting and qRT-PCR. Further inhibition of either JAK1 or STAT3 counteracted the effect of miR-210 on HUVEC proliferation and apoptosis. These findings suggest that miR-210 promotes HUVEC proliferation, at least in part, by targeting the JAK1-STAT3 signaling axis.ConclusionsThis study provides insights into the contribution of the miR-210-JAK1-STAT3 axis and its underlying mechanisms to ASO pathology.


2021 ◽  
Author(s):  
Kun Zhang ◽  
Wei Song ◽  
Jiaxuan Feng ◽  
Haoshan Qi ◽  
Mingjin Guo

Abstract Background and aims: Cholesterol crystals have been proved to be able to cause inflammation. NLRP3 inflammasome is activated in response to the accumulation of cholesterol crystals to produce IL-1β, which is associated with atherosclerotic lesions. NLRP3, as part of innate immunity, is involved in the regulation of inflammatory activity described above. The main objective of this study was to investigate the relationship between the polymorphism of NLRP3 rs10754558 and the susceptibility to arteriosclerosis obliterans (ASO) in Chinese Han males. Methods: The NLRP3 rs10754558 genotype was detected by the TaqMan allele assays in 758 male patients suffered from arteriosclerosis obliteration and 793 male controls. Blood glucose, total cholesterol (TC), triglyceride (TG), urea nitrogen, creatinine, serum uric acid, high-density lipoprotein, low-density lipoprotein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and IL-1β were detected in all subjects. Clinical data were recorded and genotype-phenotype was analyzed. Independent sample T test was used for comparison between the two groups. The odds ratio (ORs) with 95% confidence interval (CIs) was calculated to show the strength of the relationship between the genotype distribution or allele frequencies and ASO. Genotype-phenotype analysis was performed in ASO patients by ANOVA. Results: The frequencies of genotype and allele in ASO group were significantly different from that in control group (p = 0.022 by genotype, p = 0.003 by allele). People who were carrying genotype GG had a higher risk for ASO than those carrying genotype CC (OR=1.574,95%CI 1.161-2.135,P=0.003) ,which was still significant after the adjustment of the history of smoking, TC, LDL, fasting blood glucose, systolic blood pressure and BMI(OR=1.448,95%CI 1.046-1.461,P=0.004). Moreover, there was an interaction between rs10754558 of NLRP3 and rs2043211 of CARD8 gene. Under the premise of carrying the T allele of CARD8 rs2043211, the G allele of NLRP3 rs10754558 increases the susceptibility to ASO. This gene-gene interaction is consistent with IL-1β levels. Conclusion: Our finding suggests the polymorphisms of NLRP3 rs10754558 are probably associated with the development of ASO in Chinese Han male population. And there may be an interaction between rs10754558 of NLRP3 and rs2043211 of CARD8 gene in the development of ASO.


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