Isolation of a podophyllotoxin-producing endophytic fungus from Sinopodophyllum hexandrum and the anti-S180 sarcoma activity of its fermentation broth in mice

2011 ◽  
Vol 31 (1) ◽  
pp. 12-16
Author(s):  
Yun LIU ◽  
Nong-xue QIU ◽  
Hong YIN ◽  
Hui PENG
Keyword(s):  
Endophytic Fungus ◽  
Fermentation Broth ◽  
S180 Sarcoma ◽  
Sinopodophyllum Hexandrum

scholarly journals Highly oxygenated caryophyllene-type and drimane-type sesquiterpenes from Pestalotiopsis adusta, an endophytic fungus of Sinopodophyllum hexandrum

RSC Advances ◽  
2017 ◽  
Vol 7 (46) ◽  
pp. 29071-29079 ◽  
Author(s):  
Jian Xiao ◽  
Libin Lin ◽  
Jiayao Hu ◽  
Furong Jiao ◽  
Dongzhu Duan ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Sinopodophyllum Hexandrum

Four new highly oxidized caryophyllene-type sesquiterpenes (1–4), and two new drimane-type sesquiterpenes (6, 7), along with nine known compounds have been isolated from an endophytic fungus Pestalotiopsis adusta.

scholarly journals Dihydroberkleasmin A: A New Eremophilane Sesquiterpenoid from the Fermentation Broth of the Plant Endophytic Fungus Pestalotiopsis photiniae

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1910-1916 ◽  
Author(s):  
Xiao-Long Yang ◽  
Su Zhang ◽  
Hua-Jie Zhu ◽  
Du-Qiang Luo
Keyword(s):  
Endophytic Fungus ◽  
Fermentation Broth

scholarly journals Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Xiaoying Cao ◽  
Lingxia Xu ◽  
Jingyi Wang ◽  
Mengmeng Dong ◽  
Chunyan Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Early Stage ◽  
Fold Increase ◽  
Taxus Chinensis ◽  
Biosynthesis Pathway ◽  
Taxol Biosynthesis ◽  
Key Genes ◽  
Differential Expressed Genes

Abstract Background Taxol from Taxus species is a precious drug used for the treatment of cancer and can effectively inhibit the proliferation of cancer cells. However, the growth of Taxus plants is very slow and the content of taxol is quite low. Therefore, it is of great significance to improve the yield of taxol by modern biotechnology without destroying the wild forest resources. Endophytic fungus which symbiosis with their host plants can promote the growth and secondary metabolism of medicinal plants. Results Here, an endophytic fungus KL27 was isolated from T. chinensis, and identified as Pseudodidymocyrtis lobariellae. The fermentation broth of KL27 (KL27-FB) could significantly promote the accumulation of taxol in needles of T. chinensis, reaching 0.361 ± 0.082 mg/g·DW (dry weight) at 7 days after KL27-FB treatment, which is 3.26-fold increase as compared to the control. The RNA-seq and qRT-PCR showed that KL27-FB could significantly increase the expression of key genes involved in the upstream pathway of terpene synthesis (such as DXS and DXR) and those in the taxol biosynthesis pathway (such as GGPPS, TS, T5OH, TAT, T10OH, T14OH, T2OH, TBT, DBAT and PAM), especially at the early stage of the stimulation. Moreover, the activation of jasmonic acid (JA) biosynthesis and JA signal transduction, and its crosstalk with other hormones, such as gibberellin acid (GA), ethylene (ET) and salicylic acid (SA), explained the elevation of most of the differential expressed genes related to taxol biosynthesis pathway. Moreover, TF (transcriptional factor)-encoding genes, including MYBs, ethylene-responsive transcription factors (ERFs) and basic/helix-loop-helix (bHLH), were detected as differential expressed genes after KL27-FB treatment, further suggested that the regulation of hormone signaling on genes of taxol biosynthesis was mediated by TFs. Conclusions Our results indicated that fermentation broth of endophytic fungus KL27-FB could effectively enhance the accumulation of taxol in T. chinensis needles by regulating the phytohormone metabolism and signal transduction and further up-regulating the expression of multiple key genes involved in taxol biosynthesis. This study provides new insight into the regulatory mechanism of how endophytic fungus promotes the production and accumulation of taxol in Taxus sp.

scholarly journals Sesquiterpenes Isolated from Aspergillus fumigatus, an Endophytic Fungus from Ligusticum wallichii

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Xiao-Hua Li ◽  
Han-Wen Hu ◽  
Lu Tan ◽  
Wen-Lin Wu ◽  
Zhi-Xing Cao ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Spectroscopic Analyses ◽  
The Absolute

Four sesquiterpenes, including a previously undescribed one, were isolated from the fermentation broth of an endophytic fungus Aspergillus fumigatus, which was obtained from the root of Ligusticum wallichii. Their structures were elucidated through extensive spectroscopic analyses, and the absolute configurations of 1 and 3 were elucidated through NOESY analyses combined with the calculated ECD spectra.

Cytotoxic Secondary Metabolites from Fermentation Broth of Brucea javanica Endophytic Fungus 1.2.11

2007 ◽  
Vol 2 (8) ◽  
pp. 625-631 ◽  
Author(s):  
Shirly Kumala . ◽  
Robert Utji . ◽  
Pratiwi Sudarmono . ◽  
L.B.S. Kardono .
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Brucea Javanica

Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

2021 ◽  
pp. 86-95
Author(s):  
Baiq Maylinda Gemantari ◽  
Fitra Romadhonsyah ◽  
Arief Nurrochmad ◽  
Subagus Wahyuono ◽  
Puji Astuti
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Cytotoxic Activities ◽  
Dpph Assay ◽  
Nitrobenzyl Alcohol ◽  
Layer Chromatography ◽  
Coleus Amboinicus

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R*,6S*,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.

scholarly journals Evaluation of in vitro alpha-glucosidase inhibitory, antimicrobial, and cytotoxic activities of secondary metabolites from the endophytic fungus, Nigrospora sphaerica, isolated from Helianthus annuus

2019 ◽  
Vol 69 (13) ◽  
pp. 1397-1406 ◽  
Author(s):  
Preuttiporn Supaphon ◽  
Sita Preedanon
Keyword(s):  
Antimicrobial Activity ◽  
Helianthus Annuus ◽  
Cell Lines ◽  
Cancer Cell ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Fermentation Broth ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Alpha Glucosidase

Abstract Purpose This study aimed to evaluate alpha-glucosidase inhibition and antimicrobial activity as well as cytotoxic activity of extracts from the endophytic fungus, Nigrospora sp., isolated from leaves of Helianthus annuus, which is widely cultivated for food and used as a medicinal plant. Methods The fungus (TSU-CS003) was identified based on internal transcribed spacer ribosomal DNA sequences and fungal biomass, and fermentation broth was subjected to extraction by solvents (hexane and ethyl acetate). All extracts were tested for their antimicrobial activity, alpha-glucosidase inhibition, and cytotoxicity activity. In addition, the active extract was analyzed by using gas chromatography mass spectrometry (GC-MS) Results TSU-CS003 was identified as Nigrospora sphaerica. The fermentation broth extract (BE) showed strong antimicrobial activity against Staphylococcus aureus and methicillin-resistant S. aureus (Gram-positive bacteria) with minimum inhibitory concentration (MIC) values in the range of 16–32 μg/mL and a few yeasts with MIC values ranging from 64 to 128 μg/mL, especially Talaromyces marneffei with an MIC value of 4 μg/mL. The effects of BE were observed by SEM. The results showed that this extract affected the cell morphology of T. marneffei. The half-maximal inhibitory concentration (IC50) of BE from alpha-glucosidase inhibition was recorded as 17.25 μg/mL and also showed significant cytotoxicity against A549 human cancer cell lines with an IC50 value of 22.41 μg/mL. Furthermore, BE was analyzed by using GC-MS and divided into three main compounds, including 5-pentyldihydrofuran-2(3H)-one, (Z)-methyl 4-(isobutyryloxy)but-3-enoate, and 2-phenylacetic acid. Conclusion This was the first report of the endophytic fungus N. sphaerica from H. annuus. It is a potential source of active metabolites, which gave the strong antifungal activity, antioxidant activity, and cytotoxicity to A549 cancer cell lines.

scholarly journals Novel Polyketides Produced by the Endophytic Fungus Aspergillus Fumigatus from Cordyceps Sinensis

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1709 ◽  
Author(s):  
Da-Le Guo ◽  
Xiao-Hua Li ◽  
Dan Feng ◽  
Meng-Ying Jin ◽  
Yu-Mei Cao ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
2D Nmr ◽  
Cordyceps Sinensis ◽  
Two Dimensional ◽  
One Dimensional ◽  
Ic50 Values

Five new polyketides, including two pairs of enantiomers and a racemate, were isolated from the fermentation broth of Aspergillus fumigatus, an endophytic fungus isolated from Cordyceps sinensis. Their structures were identified using one-dimensional (1D) and two-dimensional (2D) NMR experiments, and the absolute configurations of the enantiomers were confirmed using electronic circular dichroism (ECD) calculations. Compounds 1a and 2a exhibited inhibitory activity against the MV4-11 cell line in vitro, with IC50 values of 23.95 µM and 32.70 µM, respectively.

scholarly journals Optimal extraction, purification and antioxidant activity of total flavonoids from endophytic fungi of Conyza blinii H. Lév

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11223
Author(s):  
Shuheng Zhao ◽  
Xulong Wu ◽  
Xiaoyu Duan ◽  
Caixia Zhou ◽  
Zhiqiao Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Antioxidant Activities ◽  
Fermentation Broth ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Macroporous Resin ◽  
Clearance Ratio

Background Flavonoids are widely used in the market because of their antibacterial, antiviral, and antioxidant activities. But the production speed of flavonoids is limited by the growth of plants. CBL9 (Chaetomium cruentum) is a flavonoid-producing endophytic fungi from Conyza blinii H. Lév, which has potential to produce flavonoids. Methods In this study, we isolated total flavonoids from endophytic fungus CBL9 of Conyza blinii H. Lév using macroporous resin D101. The process was optimized by response surface and the best extraction process was obtained. The antioxidant activities of total flavonoids were analyzed in vitro. Results It was found that the best parameters were 25 °C pH 2.80, 1.85 h, and the adsorption ratio reached (64.14 ± 0.04)%. A total of 60% ethanol was the best elution solvent. The elution ratio of total flavonoid reached to (81.54 ± 0.03)%, and the purity was 7.13%, which was increased by 14.55 times compared with the original fermentation broth. Moreover its purity could rise to 13.69% after precipitated by ethanol, which is very close to 14.10% prepared by ethyl acetate extraction. In the antioxidant research, the clearance ratio of L9F-M on DPPH, ABTS, •OH, •O2−, (96.44 ± 0.04)% and (75.33 ± 0.03)%, (73.79 ± 0.02)%, (31.14 ± 0.01)% at maximum mass concentration, was higher than L9F. Conclusion The result indicated using macroporous resin in the extraction of total flavonoid from endophytic fungus is better than organic solvents with higher extraction ratio, safety and lower cost. In vitro testing indicated that the flavonoid extracted by macroporous resin have good antioxidant activity, providing more evidence for the production of flavonoid by biological fermentation method.

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