scholarly journals Uji Aktivitas Antioksidan Minyak Argan (Argania spinosa L.) Dalam Bentuk Sediaan Mikroemulsi

2021 ◽  
Vol 1 (1) ◽  
pp. 30-39
Author(s):  
Nur Ain Thomas ◽  
Adiva Sidangoli
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
P Value ◽  
Freeze Thaw ◽  
Argania Spinosa ◽  
Globule Size ◽  
Ic50 Value ◽  
Dpph Method ◽  
Oil Water

Argan oil (Argania spinosa L.) is an oil that has benefits as a natural antioxidant which is good for health. Microemulsion is a thermodynamically stable delivery system;  transparent;  has a small globule size and consists of a mixture of oil, water, surfactant and co-surfactant which has great potential in delivering dermal active substances by increasing transdermal permeability in topical drug delivery. This research aimed to formulate argan (Argania spinosa L.) oil into microemulsion dosage form and determine the antioxidant activity using DPPH method. The study began with the optimization of base into several concentration variations of surfactant and co-surfactant. The formula made into 7 namely F1, F2, F3, F4, F5, F6 and F7, evaluated using organoleptic test and centrifugation test. The results showed the base of F6 which had the clear, transparent physical appearance and no separation occured. The F6 base was then made into microemulsion dosage with 3 concentration variations of argan (Argania spinosa L.) oil, namely F1 (0.5%), F2 (1%) and F3 (1.5%). The 3 formulas were tested for the physical stability which included pH test, viscosity test, centrifugation test and freeze-thaw test.  The testing of antioxidant activity carried out by in vitro using the DPPH method which then calculated for the IC50 value on t0 and t28. From the results of the physical stability test at room temperature and freeze-thaw, the 3 formulas met the organoleptic, pH and centrifugation tests. The antioxidant activity values of IC50 were F1 (t0 = 291.14 g/mL; t28 = 230.43 g/mL), F2 (t0 = 89.02 g/mL; t28 = 129.01 g/mL), F3 (t0 = 81.07 g/mL; t28 = 116.30 g/mL). The statistical test result of T test showed the p value = 0,896, (0.05), which indicated that there was no significance difference in the antioxidant activity result between the 3 formulas on t0and t28.

scholarly journals Uji Stabilitas Dan Aktivitas Antioksidan Mikroemulsi Likopen Tomat (Solanum lycopersicum L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 10-17
Author(s):  
Evi Sulastri ◽  
Mohamad Ikram ◽  
Yuliet Yuliet
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Tween 80 ◽  
Antioxidant Power ◽  
Solubility In Water ◽  
Solanum Lycopersicum L ◽  
Globule Size ◽  
Ic50 Value ◽  
Physical And Chemical

Lycopene is a class of keratonoid that have been proven has antioxidant activity but has limited solubility in water. So as to boost their solubility, in this study conducted in the form of microemulsion formulations. This study aims to get a tomato lycopene extract microemulsion which is stable and has antioxidant effects. The research was initiated to test the antioxidant power of tomato lycopene extract in vitro against DPPH. Tomato lycopene extract microemulsion is formulated based on the optimization of the composition of the base consisting of the VCO as the oil phase, tween 80 as surfactant and glycerin as cosurfactant. Preparations produced were tested for antioxidant activity and their physical and chemical stability testing which include: organoleptic, viscosity, globule size and pH during 28 days of storage. The results showed that tomato lycopene extract formula microemulsion stable composition of tomato lycopene extract 0.3%, VCO 15%, tween 80% and 35% glycerin. The test results obtained physical stability of the microemulsion formula elected tomato lycopene extract has a distinctive scent of aromatic, orange, thick, globule size of <5 μm, viscosity (600, 33 ± 69.29 to 746 cPs ± 8.32), and pH ( 6.27 ± 0.20 to 6.79 ± 0.10). As very strong antioxidant activity with IC50 value of 29.07 ppm.

scholarly journals Evaluation of in vitro Antioxidant and Cytotoxic Effect of Methanol Extract from aerial parts of Myristica beddomei King ssp. ustulata W.J. de Wilde

2020 ◽  
pp. 115-121
Author(s):  
Neethu Joy ◽  
Mahesh Mohan
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cytotoxic Effect ◽  
Total Phenolic ◽  
Ovarian Cancer Cells ◽  
Cytotoxicity Test ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Dpph Method

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81   to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1.  In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

scholarly journals Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

2019 ◽  
Vol 8 (2S7) ◽  
pp. 87-89
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Value ◽  
Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

scholarly journals An Indian Folkore Phytomedicine Perilla frutescens L.: Free Radical Scavenging Property along with Metal Detection by ICP-MS

2021 ◽  
Vol 33 (4) ◽  
pp. 945-948
Author(s):  
Ajay Singh ◽  
Arunesh Kumar Dixit ◽  
S. Farooq ◽  
Zafar Mehmood ◽  
Suman Lata Chhimwal
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Perilla Frutescens ◽  
Considerable Proportion ◽  
Ic50 Value ◽  
Dpph Method

In present study, free radical scavenging activity of methanolic extract of all parts (leaves, seeds, root, stem and flower) of Perilla frutescens L. has been estimated. The promising results, among the five plant parts leaves, seeds, root, stem, flower and the standard tested for the in vitro antioxidant activity using the DPPH method, the crude methanolic extracts of all parts showed antioxidant activity, with IC50 values of 5.95 ± 0.10, 8.28 ± 0.20, 66.27 ± 0.17, 80.03 ± 0.10 and 122.35 ± 0.17 μg/mL, respectively. The IC50 value for ascorbic acid was 5.19 ± 0.26 μg/mL. While butylated hydroxyl toluene (BHT) a synthetic commercial antioxidant has comparatively high IC50 value of 108.46 ± 0.57 μg/mL. Presence of zinc in all parts of plant in the range 17.20 ± 0.22 to 33.56 ± 0.32 ppm further supports the strong antioxidant activity of Perilla frutescens L. The phytochemical tests indicated that all parts of Perilla frutescens L. have considerable proportion of important phytochemicals and are in rich source of secondary metabolites like polyphenols, tannins, alkaloids and flavonoids. Several of such compounds are known to possess potent antioxidant activity.

Photostability Study on Character and Antioxidant Activity of Tomato Extract (Solanum lycopersicum l.) in Nanostructured Lipid Carrier (NLC) and Conventional Creame

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Noorma Rosita ◽  
Dewi Haryadi ◽  
Tristiana Erawati ◽  
Rossa Nanda ◽  
Widji Soeratri
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Nanostructured Lipid Carrier ◽  
Uvb Radiation ◽  
K Value ◽  
Antioxidant Power ◽  
Solanum Lycopersicum L ◽  
Tomato Extract ◽  
Dpph Method ◽  
High Shear Homogenization

The aim of this study was to investigate the ability of NLC in increasing photostability of tomato extract in term of antioxidant activity. Photostability testing on antioxidant activity of samples were conducted by accelerating method using UVB radiation 32.400 joule for 21 hours radiation. Antioxidant activity was measured by DPPH method. NLC was made by High Shear Homogenization (HPH) method at 24000 rpm for 4 cycles, while conventional creame was made by low speed at 400 rpm. The product were characterized include: pH, viscosity, and particle size. There were had difference characters and physical stability. NLC had smaller size, more homogenous and more stable than conventional creame. It was known that stability of antioxidant activity of tomato extract in NLC system higher than in conventional creame. That was showed with k value, as constanta of rate scavenging activity decreasing in antioxidant power between time (Sigma 2-tail less than 0.005) of NLC and conventional creame were: 2.03x10-2 %/hour ±0.08 (3.94) and 4.71x 10-2 %/ hour ±0.23 (4.88) respectively.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Elvi Kustiyah ◽  
Bungaran Saingin ◽  
Hernowo Widodo ◽  
Viriya Piti
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Protein Content ◽  
Green Algae ◽  
Red Algae ◽  
Marine Resources ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Lowry Method

 Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

scholarly journals The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2739
Author(s):  
Liza Devita ◽  
Hanifah Nuryani Lioe ◽  
Mala Nurilmala ◽  
Maggy T. Suhartono
Keyword(s):  
Antioxidant Activity ◽  
In Silico ◽  
Antioxidant Activities ◽  
In Silico Analysis ◽  
Weight Fraction ◽  
Amino Acid Sequences ◽  
Skin Collagen ◽  
Dpph Method ◽  
Peptide Fractions

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

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