scholarly journals Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

2019 ◽  
Vol 8 (2S7) ◽  
pp. 87-89
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Value ◽  
Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Siti Warnasih ◽  
Diana Widiastuti ◽  
Uswatun Hasanah ◽  
Laksmi Ambarsari ◽  
Purwantiningsih Sugita
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Palm Fruit ◽  
Ic50 Value ◽  
Dpph Method ◽  
Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

scholarly journals Determination of antiradical activity and phenolic and flavonoid contents of extracts and fractions of jackfruit (Artocarpus heterophyllus Lamk) seeds

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 36-43
Author(s):  
Zubaydah ◽  
M.H. Sahumena ◽  
W.O.N. Fatimah ◽  
Sabarudin ◽  
M. Arba ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Artocarpus Heterophyllus ◽  
Water Fraction ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Jackfruit (Artocarpus heterophyllus Lamk) is a plant from the Moraceae family that is widespread in Indonesia. Empirically, jackfruit can be used to cure hypertension, diabetes, cancer, asthma, dermatosis, coughs, wounds, acne, and diarrhea. The bioactive compounds in jackfruit include phenolics and flavonoids, which function as natural antioxidants. This research investigated the antiradical activity of jackfruit seed extracts and fractions using DPPH (2-2-diphenyl-1-picrylhydrazyl) and examined the total phenolic and flavonoid contents which may be developed as functional medicines and foods. The jackfruit seed powder was extracted using the maceration method. Radical scavenging activities were measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile, the contents of flavonoids were determined using the aluminum chloride complex colorimetric method. The IC50 value of ethyl acetate fraction, methanol extract, n -hexane fraction, and water fraction was 5.435±0.064 µg/mL, 5.639±0.302 µg/mL, 7.201±0.475 µg/mL, and 9.134±0.2911 µg/mL, respectively. The phenolic and flavonoid contents of ethyl acetate fraction, methanol extract, n-hexane fraction, and water fraction were 49.597±1.589, 47.949±1.966, 41.214±4.354 and 35.504±0.913 g GAE/100 g of sample, respectively for phenolic, and 70.199±0.458, 65.228±0.615, 59.907±0.719, and 54.234±0.351 g of quercetin equivalent/100 g of sample, respectively of flavonoids, with a correlation value (R2 ) to antiradical power of 0.4582 for phenolic and 0.5281 for flavonoids. The ethyl acetate fraction of jackfruit seeds can be further developed as an anti -radicals and functional food.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals α-Amilase Inhibitory Activity of Fraction of Ethanolic Extract of Lebui Seed (Cajanus cajan (L.) Millsp.) Grown in West Nusa Tenggara

2020 ◽  
pp. 266-272
Author(s):  
Dian Resti Setyaningrum ◽  
Rumiyati ◽  
Yekti Asih Purwestri ◽  
Satrijo Saloko ◽  
Agung Endro Nugroho ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Alpha Amylase ◽  
Hexane Fraction ◽  
Inhibition Activity ◽  
Water Fraction ◽  
Ic50 Value ◽  
Bioactive Content ◽  
Amylase Inhibitors

Diabetes is a metabolic syndrome characterized by high blood sugar levels or hyperglycemia.Alpha-amylase inhibitors can be used as an antidiabetic that controls postprandial hyperglycemia.Alpha-amylase inhibitors are found in plants as protein and non-protein bioactive content such as in lebuiseed. The purpose of this study was to determine the activity of alpha-amylase inhibition of the fraction ofethanol extract of lebui seed. This research was carried out by extraction of lebui seed and then fractionatedlebui extract to obtain n-hexane fraction (HF), ethyl acetate fraction (EAF), and water fraction (WF). Thefractions then tested to alpha-amylase inhibition activity in vitro by measuring the reducing sugar using3.5-dinitrosalicylic (DNS) reagent. Furthermore, the identification of the bioactive content of each fraction wascarried out using thin layer chromatography. The results of alpha-amylase inhibition activity test showed thatall fractions had the ability to inhibit alpha-amylase. The IC50 value of the n-hexane fraction, ethyl acetatefraction, and water fraction were 173.05 mg/ml, 9.98 mg/ml, and 137.19 mg/ml, respectively. Ethyl acetatefraction has the smallest IC50 value compared to other fractions. Based on the identification of the bioactivecontent, bioactive content that is only found in the ethyl acetate fraction but not in other fractions are tannins.Therefore, the chemical content in lebui seed which acts as an alpha-amylase inhibitor might be tannins.

scholarly journals ANTIOXIDANT ACTIVITY OF TAUCO ETHANOL EXTRACT AND ITS FRACTIONS

2017 ◽  
Vol 10 (14) ◽  
pp. 57
Author(s):  
Resmi Mustarichie ◽  
Sandra Megantara ◽  
Wiwiek Indriyati ◽  
Ade Zuchrotun
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Extraction Methods ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Modified Method ◽  
Ic50 Value

Objective: To investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate and n-hexane from tauco.Methods: Two types of tauco was extracted using soxhletation methods, followed by fractionation using liquid-liquid extraction methods and phytochemical screening. Antioxidant activity test was carried out using DPPH with Ascorbic acid (vitamin C) as a reference. Modified method of Farnsworth was applied for phytochemical screening.Results: It was found that extracts of ethanol and ethyl acetate fraction containing flavonoids, monoterpenoid, and sesquiterpenoids whereas the water fraction and a fraction of n-hexane only contain monoterpenes and sesquiterpenoids. The IC50 value for the ethanol extract, water, ethyl acetate, and n-hexane fractions of two taucos in a row were 1192.71 ppm, 1746.01 ppm, 722.38 ppm, 1845.45 ppm and 1190,15 ppm, 1740.30, 710.46, for tauco A and B respectively.Conclusion: It was unexpected that tauco ethanol extract and fractions showed much weaker antioxidant activity than vitamin C, which had the IC50 value of 4.41 ppm. 

scholarly journals Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

2016 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Liza Pratiwi ◽  
Achmad Fudholi ◽  
Ronny Martien ◽  
Suwidjiyo Pramono
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Garcinia Mangostana ◽  
Ic50 Value ◽  
Dpph Method ◽  
Peel Extract

<span>Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen  peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. </span><span lang="EN">The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction.</span><span> The</span><span lang="EN"> antioxidant activity </span><span>test </span><span lang="EN">using DPPH method with UV-Vis spectrophotometer</span><span>. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.</span>

scholarly journals Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

2015 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Nyi Mekar Saptarini ◽  
Irma Erika Herawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Ficus Benjamina ◽  
Water Fraction ◽  
Free Radical Formation ◽  
Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as <em>Ficus benjamina </em>and <em>Annona reticulata</em>. The study aimed to compare the antioxidant activity of extracts and fractions of <em>Ficus benjamina </em>and <em>Annona reticulata</em> leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC<sub>50</sub> values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC<sub>50</sub> value of <em>Ficus benjamina </em>was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC<sub>50</sub> value of <em>Annona reticulata</em> was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the <em>Ficus benjamina</em> water fraction was the best antioxidant compared to other extract and fraction.

Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

2015 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Nyi Mekar Saptarini ◽  
Irma Erika Herawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Ficus Benjamina ◽  
Water Fraction ◽  
Free Radical Formation ◽  
Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as <em>Ficus benjamina </em>and <em>Annona reticulata</em>. The study aimed to compare the antioxidant activity of extracts and fractions of <em>Ficus benjamina </em>and <em>Annona reticulata</em> leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC<sub>50</sub> values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC<sub>50</sub> value of <em>Ficus benjamina </em>was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC<sub>50</sub> value of <em>Annona reticulata</em> was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the <em>Ficus benjamina</em> water fraction was the best antioxidant compared to other extract and fraction.

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