scholarly journals Synthesis and Comparative Characterization of Different Microparticles used as Biomaterials in Dentistry

2021 ◽  
Vol 58 (2) ◽  
pp. 192-200
Author(s):  
Sergiu Alexandru Tofan ◽  
Cristian Olteanu ◽  
Camelia Szuhanek ◽  
ramona Amina Popovici ◽  
Magda Mihaela Luca ◽  
...  
Keyword(s):  
Living System ◽  
In Vitro Cytotoxicity ◽  
Functional Requirements ◽  
Clinical Evaluations ◽  
Polymeric Microparticles ◽  
Great Availability ◽  
Low Cytotoxicity ◽  
Materials Used

A biomaterial must be biologically compatible, mechanical, functional, corrosion resistant and easily adapt to clinical and laboratory technologies. Dental biomaterials are materials used to replace a part of a living system or to work closely with living tissue. Many scientific articles present different polymeric biocomposites with possible application in dentistry and this is a proof of the opportunity of a research in a field in full ascent and with great availability in the promotion of materials destined to �work under biological constraint� and which must also meet the functional requirements of a dental implant. The objectives of this research were to obtain and to comparatively evaluate different polymeric microparticles that can be used in dentistry. The samples based on poly(lactic-co-glycolic acid) and respectively polyurethane microparticles were characterized by pH and Zetasizer measurements, and in vitro cytotoxicity assays. The results indicate the obtaining of particles with a neutral pH, medium homogeneity, and with different tendencies to form agglomerations. Their low cytotoxicity, tested on the primary human gingival fibroblasts by MTT and LDH techniques, indicates that these microparticles are safe to be tested in further clinical evaluations.

scholarly journals In vitro cytotoxicity of Aspilia pluriseta Schweinf. extract fractions

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sospeter N. Njeru ◽  
Jackson M. Muema
Keyword(s):  
Biological Activities ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Root Extract ◽  
Lack Of Information ◽  
Information Gap ◽  
Diphenyltetrazolium Bromide ◽  
Potential Use

Abstract Objectives We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on its cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemical characterization of A. pluriseta root extracts, here we report on cytotoxicity of tested solvent fractions. We evaluated the potential cytotoxicity of these root extract fractions on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results We show that all solvent extract fractions (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivities are within the acceptable cytotoxicity and selective index limits. This finding scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.

Up-conversion emission and in vitro cytotoxicity characterization of blue emitting, biocompatible SrTiO3 nanoparticles activated with Tm3+ and Yb3+ ions

RSC Advances ◽  
2016 ◽  
Vol 6 (45) ◽  
pp. 39469-39479 ◽  
Author(s):  
R. Pazik ◽  
A. Zięcina ◽  
B. Poźniak ◽  
M. Malecka ◽  
L. Marciniak ◽  
...  
Keyword(s):  
Particle Size ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Ion Release ◽  
Size Dependent ◽  
U2os Cells

Blue emitting, up-converting NP's of SrTiO3:Tm3+/Yb3+ synthesized using the citric route are biocompatible towards J774.E whereas the cytotoxic effect to U2OS cells is not particle size dependent but most probably is related to Sr2+ ion release.

scholarly journals Synthesis, Characterization and in vitro Antimicrobial, Anticancer Activity of Random Copolyester using Orcinol

2021 ◽  
Vol 33 (9) ◽  
pp. 2119-2126
Author(s):  
T. Sivaramakrishnan ◽  
G. Elango
Keyword(s):  
In Vitro Cytotoxicity ◽  
Diffusion Method ◽  
Dsc Studies ◽  
Repeat Units ◽  
Nmr Techniques ◽  
Crystalline Nature ◽  
Uv Visible ◽  
Tga And Dsc

In present study, a novel random copolyester i.e. poly(3-oxy-5-methylphenyl-4-oxynaphthal-1- yl)isophthalate (PONI) was synthesized through solution polycondensation methodology involving orcinol, 1,4-naphthalene diol and isophthaloyl chloride and its solubility in common solvents along with viscosity measurements were performed. The structure of the repeat units in the polyester chain were ascertained based on the spectral characterization of UV-visible, FTIR, 1H & 13C NMR techniques. Thermal analysis of the copolyester were performed by TGA and DSC studies. The activation energy for thermal decomposition of polyester was estimated by well-known kinetic methods. The surface morphology and crystalline nature of the polymer were explored by employing SEM and WAXD methods. The antimicrobial efficacy of copolyester was assessed through well-diffusion method using a Gram-positive and a Gram-negative bacteria. The in vitro cytotoxicity of the polyester prepared was verified against HeLa cell line using MTT assay.

DEVELOPMENT AND CHARACTERIZATION OF A NANOCARRIER FOR QUERCETIN

2009 ◽  
Vol 08 (01n02) ◽  
pp. 175-179 ◽  
Author(s):  
MAN-YI WONG ◽  
GIGI N. C. CHIU
Keyword(s):  
Breast Cancer Cells ◽  
Water Solubility ◽  
Storage Temperature ◽  
In Vitro Cytotoxicity ◽  
Cytotoxic Compound ◽  
Naturally Occurring ◽  
Quercetin Concentration ◽  
Fold Stability

Quercetin is a naturally occurring cytotoxic compound where clinical use has been limited by its low water solubility. Therefore, liposomes were explored for solubilizing quercetin. Liposomes composed of DPPC (1,2 dipalmitoyl-sn-glycerol-3-phosphocholine)/DSPE-PEG2000 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)2000])/quercetin (90:5:5 mole ratio) incorporated quercetin efficiently at 100.9 ± 4.6% and increased quercetin concentration in water 11.2-fold. Stability studies at storage temperature of 4°C showed that the liposomes were stable for up to 16 weeks, without any significant changes in diameters. Liposomal quercetin showed a delayed release profile and reduced quercetin degradation. In vitro cytotoxicity tests also showed that the ED50 of liposomal quercetin was 17.6 times lower than free quercetin in MDA-MB-231 breast cancer cells. In conclusion, the DPPC/DSPE-PEG-based liposomes were stable and were capable of solubilizing quercetin, preventing quercetin degradation, and increasing quercetin in vitro cytotoxicity. Hence, liposomes are a suitable nanocarrier for quercetin.

scholarly journals Lymphocyte in vitro cytotoxicity: Characterization of mouse lymphotoxin

1970 ◽  
Vol 1 (1) ◽  
pp. 122-132 ◽  
Author(s):  
W.P. Kolb ◽  
G.A. Granger
Keyword(s):  
In Vitro Cytotoxicity

Synthesis and characterization of benzotriazolyl acrylonitrile analogs-based donor-acceptor molecules: optical properties, in vitro cytotoxicity, and cellular imaging

2021 ◽  
pp. 109251
Author(s):  
Eugenio Hernández-Fernández ◽  
Ana Sofia Ortega-Villarreal ◽  
Ma. Concepción García-López ◽  
Rodrigo Chan-Navarro ◽  
Samuel Garrard ◽  
...  
Keyword(s):  
Optical Properties ◽  
Cellular Imaging ◽  
In Vitro Cytotoxicity ◽  
Synthesis And Characterization ◽  
Donor Acceptor ◽  
Acceptor Molecules
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