Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation

2017 ◽  
Vol 68 (2) ◽  
pp. 323-327
Author(s):  
Cristian Levente Giuroiu ◽  
Maria Vataman ◽  
Gabriel Melian ◽  
Dragos Bularda ◽  
Ludmila Lozneanu ◽  
...  
Keyword(s):  
Dental Caries ◽  
Interleukin 6 ◽  
Dental Pulp ◽  
The Other ◽  
Control Group ◽  
Study Group ◽  
Pulp Tissue ◽  
Immunohistochemical Technique ◽  
Systemic Distribution ◽  
Cell Zone

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.

scholarly journals The Association of Circulating MiR-29b and Interleukin-6 with Subclinical Atherosclerosis

2017 ◽  
Vol 44 (4) ◽  
pp. 1537-1544 ◽  
Author(s):  
Yu-qing Huang ◽  
Jie Li ◽  
Ji-yan Chen ◽  
Ying-ling Zhou ◽  
An-ping Cai ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Multiple Linear Regression ◽  
Interleukin 6 ◽  
Subclinical Atherosclerosis ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Control Group ◽  
Study Group ◽  
Circulating Levels

Background/Aims: Although it is widely acknowledged that atherosclerosis is mainly a chronic inflammatory process, in which both miR-29b and interleukin-6 (IL-6) play multifaceted roles, the association between miR-29b and IL-6 remains unknown. The aim of the present study was to explore the relationship between miR-29b and IL-6 and to test whether circulating levels of miR-29b and IL-6 could predict atherosclerosis. Methods: A total of 170 participants were divided into two groups according to carotid intima-media thickness (CIMT): study group (CIMT ≥ 0.9mm) and control group (CIMT < 0.9mm). Levels of circulating miR-29b and IL-6 were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The association of miR-29b and IL-6 levels with CIMT was assessed using Spearman correlation analysis and multiple linear regression analysis. Results: The study group showed higher miR-29b levels (31.61 ± 3.05 vs. 27.91 ± 1.71 Ct, p < 0.001) and IL-6 levels (3.40 ± 0.67 vs. 2.99 ± 0.37 pg/ml, p < 0.001), compared with the control group. CIMT was positively correlated with miR-29b (r = 0.587, p < 0.001) and IL-6 (r = 0.410, p < 0.001), and miR-29b levels were also correlated with IL-6 (r = 0.242, p = 0.001). Multiple linear regression analysis also showed that CIMT was positively correlated with miR-29b and IL-6. After adjustment for age, body mass index, systolic blood pressure, total cholesterol and C-reactive protein, CIMT was still closely correlated with miR-29b and IL-6. The combination of miR-29b and IL-6 (AUC = 0.901, p < 0.001) offered a better predictive index for atherosclerosis than either miR-29b (AUC = 0.867, p < 0.001) or IL-6 (AUC = 0.747, p < 0.001) alone. Conclusion: Circulating levels of miR-29b and IL-6 may be independently correlated with subclinical atherosclerosis, and may serve as novel biomarkers for the identification of atherosclerosis.

scholarly journals A Randomised-Controlled Clinical Study Examining the Effect of High-Intensity Laser Therapy (HILT) on the Management of Painful Calcaneal Spur with Plantar Fasciitis

2021 ◽  
Vol 10 (21) ◽  
pp. 4891
Author(s):  
Piotr Tkocz ◽  
Tomasz Matusz ◽  
Łukasz Kosowski ◽  
Karolina Walewicz ◽  
Łukasz Argier ◽  
...  
Keyword(s):  
Pain Management ◽  
High Intensity ◽  
Plantar Fasciitis ◽  
Heel Pain ◽  
The Other ◽  
Control Group ◽  
Study Group ◽  
Calcaneal Spur ◽  
High Intensity Laser ◽  
Intensity Laser

Calcaneal spur and plantar fasciitis are the most common causes of plantar heel pain. There are many effective physical modalities for treating this musculoskeletal disorder. So far, the are no clear recommendations confirming the clinical utility of high-intensity laser therapy (HILT) in the management of painful calcaneal spur with plantar fasciitis. This study aimed to evaluate the effectiveness of HILT in pain management in patients with calcaneal spur and plantar fasciitis. A group of 65 patients was assessed for eligibility based on the CONSORT guidelines. This study was prospectively registered in the Australian New Zealand Clinical Trial Registry platform (registration number ACTRN12618000744257, 3 May 2018). The main eligibility criteria were: cancer, pregnancy, electronic and metal implants, acute infections, impaired blood coagulation, cardiac arrhythmias, taking analgesic or anti-inflammatory medications, non-experience of heel pain, or presence of other painful foot conditions. Finally, 60 patients were randomly assigned into two groups: study group (n = 30, mean age 59.9 ± 10.1), treated with HILT (7 W, 149.9 J/cm2, 1064 nm, 4496 J, 12 min), and placebo-controlled group (n = 30, mean age 60.4 ± 11.9), treated with sham HILT therapy. Both groups received ultrasound treatments (0.8 W/cm2, 1 MHz frequency, 100% load factor, 5 min). Treatment procedures were performed once a day, five times per week for three weeks (total of 15 treatment sessions). Study outcomes focused on pain intensity and were assessed before (M1) and after (M2) the treatment as well as after 4 (M3) and 12 (M4) weeks using the Visual Analogue Scale (VAS) and the Laitinen Pain Scale (LPS). According to VAS, a statistically significant decrease in the study group was observed between M1 and M2 by 3.5 pts, M1 and M3 by 3.7 pts, and M1 and M4 by 3.2 pts (p < 0.001). On the other hand, the control group showed a statistically significant decrease (p < 0.001) between M1 and M2 by 3.0 pts, M1 and M3 by 3.4 pts, and M1 and M4 by 3.2 pts. According to LPS, a statistically significant decrease in the study group was observed between M1 and M2 by 3.9 pts, M1 and M3 by 4.2 pts, and M1 and M4 by 4.0 pts (p < 0.001). On the other hand, the control group showed a statistically significant decrease between M1 and M2 by 3.2 pts (p = 0.002), M1 and M3 by 4.0 pts (p < 0.001), and M1 and M4 by 3.9 pts (p < 0.001). However, there were no statistically significant differences between the groups in VAS and LPS (p > 0.05). In conclusion, the HILT does not appear to be more effective in pain management of patients with calcaneal spurs and plantar fasciitis than the conservative standard physiotherapeutic procedures.

scholarly journals Human Dental Pulp Tissue during Orthodontic Tooth Movement: An Immunofluorescence Study

2020 ◽  
Vol 5 (3) ◽  
pp. 65 ◽  
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Giovanni Matarese ◽  
Angela Militi ◽  
Marco Matarese ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Pulp Tissue ◽  
Force Application ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

The orthodontic tooth movement is the last step of several biological processes that take place after the application of external forces. During this process, dental pulp tissue is subjected to structural and protein expression modifications in order to maintain their integrity and functional morphology. The purpose of the present work was to perform an in vivo study, evaluating protein expression modifications in the human dental pulp of patients that have undergone orthodontic tooth movement due to pre-calibrated light force application for 30 days. Dental pulp samples were extracted from molars and premolars of the control group and after 7 and 30 days of treatment; the samples were then processed for immunofluorescence reactions using antibodies against fibronectin, collagen I and vascular endothelial growth factor (VEGF). Our results show that, after 7 days of treatment, all tested proteins change their pattern expression and will reset after 30 days. These data demonstrate that the dental pulp does not involve any irreversible iatrogenic alterations, supporting the efficacy and safety of using pre-calibrated force application to induce orthodontic tooth movement in clinical practice.

Evaluation of Salivary Sialic Acid Levels in Acute Lymphoblastic Leukemic Children and Its Correlation with Dental Caries Experience

2013 ◽  
Vol 37 (3) ◽  
pp. 309-313 ◽  
Author(s):  
S Joshi ◽  
AM Hegde ◽  
K Rai ◽  
S Shetty
Keyword(s):  
Sialic Acid ◽  
Dental Caries ◽  
Linear Relationship ◽  
Caries Experience ◽  
Control Group ◽  
Study Group ◽  
Saliva Collection ◽  
The Mean

Aim- The aim of present study was to evaluate the salivary sialic acid levels in Acute Lymphoblastic Leukemic (ALL) children and to correlate it with dental caries experience. Method- A total of 120 children aged 4-10yrs were divided into 4 groups of 30 each. Dental caries experience was recorded followed by un-stimulated saliva collection. Results- The mean salivary sialic acid levels of the study group were much higher when compared with the control group, which was statistically significant. A linear relationship between salivary sialic acid levels and dental caries in leukemic children was observed. Conclusion - The findings of our study conclude that salivary sialic acid levels may have a potent activity in dental caries process and can be used as a useful marker in cancer.

Oral Health Status in Autism Patients Children in Iraq

2021 ◽  
Vol 19 (6) ◽  
pp. 54-60
Author(s):  
Raghad R. Al-Zaidi
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Oral Care ◽  
Mean Value ◽  
Permanent Teeth ◽  
Caries Experience ◽  
Control Group ◽  
Study Group ◽  
Significant Difference ◽  
And Control

Background: The autism spectrum disorder (ASD) has been introduced as one of the complicated developmental disabilities impairing communication and behavioral, intellectual as well as social functioning describes diverse symptoms, such as difficulties in communication skills and social interactions. The present research has been performed to assess seriousness of the dental caries in conjunction with the oral cleanliness amongst children suffering from autism in comparison with a control group according to age groups and gender. Materials and Methods: This research involved 30 children aged 3-14 years (male, female) who suffered from autism and attended autism centers in Welfare Children Teaching Hospital in Baghdad province, Iraq, were selected for the study compared to 30 healthy children with the same age group. Plaque (PlI), Decayed, missing, and filled surfaces (dmfs, DMFS), calculus (CI) as well as Gingival (GI) indices have been applied for measuring the status of oral health for these two groups. The data of our research has been analyzed by SPSS 26. Results: The entire autism group was caries active. For primary dentition, a lower dmfs values were recorded for study in comparison with the controls, differences have been not significant in dmfs, while for permanent dentition, a higher DMFS values were recorded for study in comparison with the control group with statistically significant concerning DMFS (P < 0.05). Moreover, caries experience (DS and DMFS) among both genders were higher in the study group in comparison with the controls with a significant difference in female only. Total mean value of PlI in the study group has been greater than the controls with no statistically significant difference. In addition, total mean value of GI in the study group has been lower than the controls with no statistically significant difference. Furthermore, correlation coefficient between the caries experience of primary and permanent teeth with PlI and GI among study and control group showed no significant correlations seen in the study and control groups, all of them were positively correlated except (ds) with (GI) in control group which was negatively correlated with no significant correlation. Conclusion: children with ASD had higher dental caries severity regarding permanent teeth compared to normal subjects, plaque higher in autistic group than control group. Dental care planners must design preventing strategies for avoiding caries; procedures for oral care as well as educational programs for oral care and promotion for addressing diverse challenges facing the oral care in ASD.

scholarly journals Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: A pilot study

2013 ◽  
Vol 84 (3) ◽  
pp. 521-526 ◽  
Author(s):  
Daniel Chavarría-Bolaños ◽  
Alan Martinez-Zumaran ◽  
Nelson Lombana ◽  
Hector Flores-Reyes ◽  
Amaury Pozos-Guillen
Keyword(s):  
Substance P ◽  
Dental Pulp ◽  
Endogenous Opioids ◽  
The Other ◽  
Pulp Tissue ◽  
Expression Levels ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Dental Pulp Tissue ◽  
Experimental Group

ABSTRACT Objective: To determine the levels of two sensory neuropeptides (substance P [SP] and calcitonin gene-related peptide [CGRP]) and two endogenous opioids (methionine-enkephalin [Met-Enk] and β-endorphin [β-End]) in dental pulp tissue samples subjected to controlled orthodontic intrusive forces. Materials and Methods: Sixteen healthy premolars were selected from eight patients who were undergoing extraction for orthodontic purposes. Eight were randomly used as controls, and the other eight were assigned to an experimental group (controlled orthodontic intrusive forces applied for 24 hours). After this period, teeth were extracted, and pulp samples were obtained. All samples were processed to quantify the expression levels of SP, CGRP, Met-Enk, and β-End using commercial radioimmunoassay kits. Results: All samples exhibited basal levels of both neuropeptides and endogenous opioids. After 24 hours of the intrusive stimulus, all patients reported a tolerable discomfort localized at the involved premolar. Only SP was significantly increased (P &lt; .05). For the other molecules, no statistically significant differences were observed (P &gt; .05); however, they expressed important increasing trends. Conclusions: The expression levels of SP and CGRP in dental pulp samples from the experimental group support the positive correlation between the symptomatic clinical scenario and increased expression levels of neuropeptides, clarifying the role of neurogenic inflammation in early injury response.

scholarly journals Increased concentrations of inflammatory mediators in unstable angina: correlation with serum troponin T

Heart ◽  
2001 ◽  
Vol 85 (5) ◽  
pp. 571-575
Author(s):  
A Mazzone ◽  
S De Servi ◽  
I Mazzucchelli ◽  
I Bossi ◽  
E Ottini ◽  
...  
Keyword(s):  
Heart Disease ◽  
Unstable Angina ◽  
Ischaemic Heart Disease ◽  
Interleukin 6 ◽  
Troponin T ◽  
Thrombus Formation ◽  
Interferon Γ ◽  
The Other ◽  
Control Group ◽  
Group 1

OBJECTIVETo measure plasma interferon γ, monocyte chemotactic protein-1 (MCP-1), and interleukin 6 and to assess their correlation with cardiac troponin T in unstable angina.DESIGNBlood sampling in patients undergoing coronary arteriography for known or suspected ischaemic heart disease.PATIENTS76 patients divided in three groups: 29 with unstable angina (group 1), 28 with stable angina (group 2), and 19 without ischaemic heart disease and with angiographically normal coronary arteries (group 3).MAIN OUTCOME MEASURESPlasma interleukin 6, interferon γ, MCP-1, and troponin T in the three groups of patients.RESULTSInterleukin 6 was increased in group 1 (median 2.19 (range 0.53–50.84) pg/ml) compared with the control group (1.62 (0.79–3.98) pg/ml) (p < 0.005), whereas interferon γ was higher in group 1 (range 0–5.51 pg/ml) than in the other two groups (range 0–0.74 pg/ml and 0–0.37 pg/ml; p < 0.005 and p < 0.001, respectively). Patients with unstable angina (group 1) and positive troponin T had higher concentrations of interferon γ than those with negative troponin T (0–5.51 pg/mlv 0–0.60 pg/ml, p < 0.001). Plasma MCP-1 was also higher in group 1 (median 267 (range 6–8670) pg/ml) than in the other two groups (134 (19–890) pg/ml and 84.5 (5–325) pg/ml; p < 0.005 and p < 0.001, respectively), and among group 1 patients with a positive troponin T assay than in those with normal troponin T (531 (14.5–8670) pg/mlv 69 (6–3333) pg/ml; p < 0.01). There was no difference in plasma interleukin 6 in group 1 patients between those with and without raised troponin T.CONCLUSIONSThe inflammatory cytokines interferon γ and MCP-1 are increased in patients with unstable angina, particularly in those with raised concentrations of troponin T, suggesting that they are probably related to myocardial cell damage or to plaque rupture and thrombus formation.

scholarly journals EFFECT OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE, LEDERMIX® ON VIABILITY OF PULP MESENCHYMAL STEM CELLS

2019 ◽  
pp. 49-53 ◽  
Author(s):  
EMIRIA DITA PRASANTI ◽  
ANGGRAINI MARGONO ◽  
NILAKESUMA DJAUHARIE
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Primary Cultures ◽  
Immunofluorescence Assay ◽  
Control Group ◽  
Third Molars ◽  
Pulp Tissue ◽  
Triple Antibiotic Paste

Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigantsand medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), andLedermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, andLedermix® on the viability of dental pulp stem cells (DPSC).Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performedto confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cellviability was analyzed using the MTT assay.Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05).Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.

The effect of local application of insulin-like growth factor for prevention of inner-ear damage caused by electrode trauma

2017 ◽  
Vol 131 (3) ◽  
pp. 245-252
Author(s):  
H Gur ◽  
Y Alimoglu ◽  
U Duzenli ◽  
S Korkmaz ◽  
S Inan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cochlear Implantation ◽  
Histopathological Examination ◽  
Round Window ◽  
Auditory Brainstem ◽  
The Other ◽  
Control Group ◽  
Study Group ◽  
Insulin Like Growth Factor ◽  
Brainstem Response

AbstractBackground:Electrode insertion during cochlear implantation causes cochlear damage and apoptosis. Insulin-like growth factor applied locally was investigated in 21 rats.Methods:In the sham group, an intracochlear dummy electrode was inserted through the round window. In the control group, after the same insertion procedure, saline-soaked porcine skin gelatine was placed on the round window. In the study group, insulin-like growth factor 1 soaked gelatine was placed on the round window. Auditory brainstem response thresholds were measured and histopathological examination was performed.Results:In the study group, at 2–4 kHz, one rat had deterioration, one showed improvement and the rest had stable thresholds 14 days after intervention. At 6 kHz, four rats showed improvement and the rest remained stable. At 8 kHz, four showed improvement, one had deterioration and two remained stable. In the other groups, hearing loss deteriorated in about half of the rats and remained stable in the rest. The mean post-operative 6 kHz threshold was significantly lower than that immediately after the intervention in the study group, contrary to the other groups. The study group had significantly better mean histopathological grading than the other groups.Conclusion:Local insulin-like growth factor 1 application may protect hearing after cochlear implantation.

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