scholarly journals Antimicrobial activity of the essential oils from the leaves and stems of Amomum rubidumLamxay & N. S. Lý

Author(s):  
Le T Huong ◽  
◽  
Nguyen T Viet ◽  
Ly N Sam ◽  
Cao N Giang ◽  
...  

This paper described the chemical compositions and antimicrobial activity of the essential oils from the leaves and stem of Amomum rubidumLamxay & N. S. Lý, collected from Bidoup Nui Ba National Park, Lam Dong, Vietnam. The essential oils were obtained by hydrodisitllation method while antimicrobial activity was evaluetd by microdilution broth susceptibility assay. The main constituents of the leaf essential oil were identified as 1,8-cineole (37.7%), -3-carene (19.5%) and limonene (16.3%) while -3-carene (21.9%), limonene (17.8%) and β-phellandrene (14.6%) dominated in the stem essentialoil. The leaf and stem essential oils displayed stronger inhibition of Pseudomonas aeruginosawith MIC of 25 μg/mLand 50 μg/mLrespectively. The stem essential oil was active against Candida albicans(MIC, 50 μg/mL) while both essential oils inhibited the growth of Fusarium oxysporum(MIC 50 μg/mL). This is the first report on chemical constituents and antimicrobial activity of the essential oils of A. rubidum.

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.


2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Taiwo Oluwafunmilola Abifarin ◽  
Gloria Aderonke Otunola ◽  
Anthony Jide Afolayan

This study was aimed at comparing the essential oils obtained from Heteromorpha arborescens leaves by Solvent-Free Microwave Extraction (SFME) and Hydrodistillation (HD) methods in terms of their chemical compositions, yield, CO2 emission, and energy consumption. The solvent-free microwave extraction method indicated a higher oil yield of 0.7 mL/200 g (0.35%) as compared to 0.59 mL/200 g (0.295%) obtained through hydrodistillation. GC-MS analysis of the oils revealed a total of 52 chemical components from both methods with the presence of 35 (96.52%) and 30 (71.15%) chemical constituents for HD and SFME, respectively. The major constituents observed in the essential oil extracted by SFME methods include α-pinene (6%), D-limonene (11.27%), β-ocimene (9.09%), β-phellandrene (6.33%), β-mycene (8.49%), caryophyllene (5.96%), and camphene (4.28%). However, in the hydrodistillation method, the oil was majorly composed of a-pinene (4.41%), β-pinene (10.68%), β-ocimene (6.30%), germacrene-D (5.09%), humulene (5.55%), and α-elemene (6.18%). The SFME method was better in terms of saving energy (0.25 kWh against 4.2 kWh of energy consumed), reduced CO2 emission (200 g against 3360 g of CO2), a higher yield, and better quality of essential oil due to the presence of higher valuable oxygenated compounds (8.52%) against that of the hydrodistillation method (2.96%). The SFME method is, therefore, a good alternative for extracting the oils of H. arborescens leaves since the essential oil yield is higher with more oxygenated compounds, considerable energy savings, lower cost, and reduced environmental burden at substantially reduced extraction time (30 min as opposed to 180 min).


2019 ◽  
Vol 25 (2) ◽  
pp. 138-144
Author(s):  
Azadeh Hamedi ◽  
Ardalan Pasdaran ◽  
Arsalan Pasdaran

Background: Different endemic species of Apiaceae that widely grow in Hyrcanian region have long been used as spices and herbal remedies. Chemical compositions and bioactivity of many of these plants have not been studied scientifically. The volatile constituents and antimicrobial activity of four edible Apiaceae plants—Elaeosticta glaucescens Boiss, Malabaila secacul (Mill.) Boiss, Caucalis platycarpos L., and Eryngium caucasicum Trautv—roots were investigated. Methods: Gas chromatography/mass spectrometry (GC–MS) and gas chromatography/flame ionization detection (GC–FID) methods were used for chemical investigation. Antimicrobial potential of the volatile compositions of these roots were investigated using the disc diffusion method on four microorganisms including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis. Results: The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these species were revealed for B. subtilis and S. aureus (500 µg/mL for MIC and MBC) followed by E. caucasicum and C. platycarpus, respectively. The main compounds of E. caucasicum root essential oil were hexyl isovalerate (11.0%) and hexyl valerate (10.1%). E. glaucescens root essential oil was mainly composed of 3(10)-caren-4-ol, acetoacetic acid ester (9.8%), octyl isovalerate (8.9%), and octyl acetate (5.7%). In C. platycarpos root, hexyl isovalerate (9.2%) and n-octyl isobutyrate (8.5%) were constituted the major compositions of the essential oil while n-hexyl isobutyrate (8.5%) and hexyl hexanoate (7.8%) were predominant compounds in M. secacul. Conclusion: The essential oils of E. caucasicum and C. platycarpus roots were contained high amount of short chain fatty acid esters (SCFAE) and trans- pinocarvyl acetate. They exhibited moderate antibacterial activity.


2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Le T. Huong ◽  
Trinh T. Huong ◽  
Nguyen T. T. Huong ◽  
Dao T. M. Chau ◽  
Ly N. Sam ◽  
...  

The chemical constituents of essential oils obtained by hydrodistillation from Zingiber vuquangensis Lý N.S., Lê T.H., Trịnh T. H., Nguyễn V.H., Đỗ N.Đ. and Zingiber castaneum Škorničk. & Q.B. Nguyễn collected from Vu Quang National Park, Hà Tĩnh Province, Vietnam were analysed by GC and GC-MS. β-Pinene (24.7% and 26.1%) and β-caryophyllene (12.3% and 13.9%) were the main constituents in the leaf oil and stem oil of Zingiber vuquangensis. On the other hand the root oil contained bornyl acetate (20.9%), zerumbone (14.1%) and α-humulene (9.6%) while β-pinene (19.6%), 1,8-cineole (15.6%), α-pinene (10.3%) and β-caryophyllene (10.4%) were the significant compounds of the fruit oil. The leaf oil Zingiber castaneum was dominated by β-pinene (30.6%), α-pinene (9.5%), β-caryophyllene (9.4%) and bicycloelemene (9.1%). The compounds occurring in higher quantity in the stem oil were β-caryophyllene (14.7%), δ-cadinene (9.8%), bicycloelemene (8.4%) and α-cubebene (7.8%). However, camphene (15.1%), 1,8-cineole (13.6%), linalool (11.3%) and δ-3-carene (8.5%) were the main compounds of the root oil while ( E)-nerolidol (23.2%), ( Z)-9-octadecenamide (17.3%) and β-caryophyllene (10.8%) were the main constituents of the fruit oil. The essential oil did not exhibit noticeable antimicrobial effects. This is the first report on the volatile compositions of Z. vuquangensis and Z. castaneum.


2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Prabodh Satyal ◽  
Bhuwan K. Chhetri ◽  
Noura S. Dosoky ◽  
Ambika Poudel ◽  
William N. Setzer

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.


2016 ◽  
Vol 13 (4) ◽  
pp. 1-7 ◽  
Author(s):  
Samuel Okhale ◽  
Chukkol Buba ◽  
Peters Oladosu ◽  
Henry Egharevba ◽  
Grace Ugbabe ◽  
...  

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 606 ◽  
Author(s):  
Dao Thi Minh Chau ◽  
Nguyen Thanh Chung ◽  
Le Thi Huong ◽  
Nguyen Huy Hung ◽  
Isiaka A. Ogunwande ◽  
...  

The Lauraceae is a family rich in aromatic and medicinal plants. Likewise, essential oils derived from members of this family have demonstrated a myriad of biological activities. It is hypothesized that members of the Lauraceae from Vietnam will yield essential oils that may be useful in controlling mosquito populations and treating microbial infections. In this work, the leaf essential oils of eleven species of Lauraceae (Beilschmiedia erythrophloia, B. robusta, B. yunnanensis, Cryptocarya concinna, C. impressa, C. infectoria, Litsea viridis, Machilus balansa, M. grandifolia, Neolitsea ellipsoidea, and Phoebe angustifolia) have been obtained by hydrodistillation and the chemical compositions analyzed by gas chromatography – mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID). The essential oils were screened for larvicidal activity against Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus, and for antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, and Candida albicans. The leaf essential oil of N. ellipsoidea, rich in (E)-β-ocimene (87.6%), showed excellent larvicidal activity against Ae. aegypti with a 24 h LC50 of 6.59 μg/mL. The leaf essential oil of C. infectoria, dominated by germacrene D (55.5%) and bicyclogermacrene (11.4%), exhibited remarkable larvicidal activity against Cx. quinquefasciatus (48 h LC50 = 0.40 μg/mL). N. ellipsoidea leaf essential oil also demonstrated notable antibacterial activity against E. faecalis and B. cereus with minimum inhibitory concentration (MIC) values of 16 μg/mL, while the leaf essential oil of C. impressa showed excellent anticandidal with an MIC of 16 μg/mL. Leaf essential oils from the Lauraceae should be considered for utilization as alternative agents for controlling mosquito populations and as antimicrobial agents.


2013 ◽  
Vol 59 ◽  
pp. 41-48 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.


2015 ◽  
Vol 61 (01) ◽  
pp. 3-11 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  

Chemical composition and antimicrobial activity of leaves and berries essential oils from Juniperus foetidissima Willd. (Cupressaceae) grown in R. Macedonia (RM) was investigated. GC/FID/MS analysis was carried out and 93 components were identified, representing 89.7-96.5% of the oils. The major components of the berries essential oil were α-pinene (19.2%), limonene (24.9%) and cedrol (23.1%), followed by smaller amounts of b-funebrene, trans-caryophyllene, germacrene D and d-cadinene. The composition of the leaves essential oil was variable depending on the region of collection. Accordingly, samples originated from southeastern RM contained essential oil with α-pinene (67.6%) and limonene (10.0%), from central part of RM with limonene (17.9-27.1%) and cedrol (28.8-33.9%), while samples from southwestern RM contained oil with terpinen-4-ol (19.1%), cis-thujone (8.3%), germacrene D (11.0%) and d-cadinene (6.3%) as predominant components in the oil. Antimicrobial screening of the essential oils was made by disc diffusion and broth dilution method against 16 bacterial strains of Gram-positive and Gram-negative bacteria and one strain of Candida albicans. The leaves essential oil showed stronger antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 ml/ml) and moderate activity against Campylobacter jejuni (MIC > 500 ml/ml). Other investigated bacterial strains and Candida albicans were completely resistant to the antimicrobial activity of J. foetidissima essential oils.


2011 ◽  
Vol 65 (4) ◽  
pp. 455-463 ◽  
Author(s):  
Ljiljana Stanojevic ◽  
Mihajlo Stankovic ◽  
Milorad Cakic ◽  
Vesna Nikolic ◽  
Ljubisa Nikolic ◽  
...  

The essential oils from flowers of Lavandula officinalis L. (L. officinalis L. = L. angustifolia Mill.) were obtained by two hydrodistillation techniques: standard (technique I) and modified (technique II) Clevenger hydrodistillation. The yield, hydrodistillation kinetics, composition and antimicrobial activity of essential oils were investigated. The higher yield of oil was obtained by hydrodistillation technique II. The new analytical hydrodistillation kinetics model of essential oil from Lavandulae flowers was defined. The essential oil composition was analyzed by gas chromatography-mass spectroscopy (GC-MS). There are differences in chemical compositions of the oils obtained by different techniques. Antimicrobial activity of essential oil obtained by technique I and II against Salmonella enteritidis, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Candida albicans and Aspergillus niger is the same, while the activity against Escherichia coli and Pseudomonas aeruginosa is slightly different.


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