Reduction of Milk Contamination with Aflatoxin-M1 through Vaccination of Dairy Cattle with Aflatoxin-B1 Vaccine

2020 ◽  
Keyword(s):  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Nano Particles ◽  
Aflatoxin M1 ◽  
Serum Samples ◽  
Serum Albumins ◽  
Milk Contamination ◽  
Before And After

Aflatoxin M1 is one of mycotoxin derivatives, which is secreted in milk of dairy cattle fed on feed contaminated with Aflatoxin-B1 (AFB1). The current study was designed to prepare a vaccine against AFB1and to evaluate its efficacy in reducing or preventing secretion of AFM1 in milk. Aflatoxin-B1 was prepared, purified and transformed into oxime, then it was fixed on bovine serum albumins. The AFB1-BSA conjugate was adjuvanted with Gold Nano particles then Montanide ISA 206. The prepared vaccine was used for immunization of rabbits by S/c routes as 100 µg/dose and dairy cattle by I/M routes as 500 µg/dose. The vaccinated animals were boosted at 3 weeks post primary immunization. Serum samples were collected and examined for the anti-AFB1 using AGPT. A mean titer of 15.2 AGPU/ml was detected at 2 weeks post primary vaccination then significantly increased till reached to 76.8 AGPU/ml at 6 weeks post Booster vaccination. All vaccinated rabbits were challenged with dose of 0.3 mg AFB1 toxin/Kg. The vaccinated rabbit showed 100% protection and no AFB1 toxin residue was detected in their livers. Milk samples were collected from non-vaccinated and AFB1-immunized dairy cattle then examined with ELISA for quantitation of AFM1 residues before and after vaccination. The results showed that the prepared AFB1 vaccine was safe, potent and able to reduce AFM1 release in milk of vaccinated heifers by 70%. So the vaccination of lactating animals with the AFB1vaccine might represent a valid tool for the prevention of AFM1 contamination of milk and dairy products.

scholarly journals Serological responses to COVID-19 booster vaccine in England

2021 ◽  
Author(s):  
Georgina Ireland ◽  
Heather Whitaker ◽  
Shamez N Ladhani ◽  
Frances Baawuah ◽  
Vani Subbarao ◽  
...  
Keyword(s):  
Geometric Mean ◽  
Vaccine Dose ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Serum Samples ◽  
National Immunisation ◽  
Primary Immunisation ◽  
Vaccine Type ◽  
Before And After ◽  
Ongoing Surveillance

Importance: There are limited data on immune responses after COVID-19 vaccine boosters in individuals receiving primary immunisation with BNT162b2 (Pfizer-BioNTech) or AZD1222 (AstraZeneca) Objective: To assess SARS-CoV-2 antibody responses before and after booster vaccination with BNT162b2 in adults receiving two BNT162b2 or AZD1222 vaccine doses at least 6 months previously, as part of the United Kingdom national immunisation schedule Design: Prospective, cohort study Setting: London, England Participants: 750 immunocompetent adults aged ≥50 years Interventions: A single dose of BNT162b2 administered at least six months after primary immunisation with two doses of BNT162b2 given <30 days apart (BNT162b2-control) or ≥30 days apart (BNT162b2-extended) compared to AZD1222 given ≥30 days apart (AZD1222-extended) Main Outcome and Measures: SARS-CoV-2 spike protein antibody geometric mean titres (GMTs) before and 2-4 weeks after booster Results: Of 750 participants, 626 provided serum samples for up to 38 weeks after their second vaccine dose. Antibody GMTs peaked at 2-4 weeks after the second dose, before declining by 68% at 36-38 weeks after dose 2 for BNT162b2-control participants, 85% at 24-29 weeks for BNT162b2-extended participants and 78% at 24-29 weeks for AZD1222-extended participants. Antibody GMTs was highest in BNT162b2-extended participants (942 [95%CI, 797-1113]) than AZD1222-extended (183 [124-268]) participants at 24-29 weeks or BNT162b2-control participants at 36-38 weeks (208; 95%CI, 150-289). At 2-4 weeks after booster, GMTs were significantly higher than after primary vaccination in all three groups: 18,104 (95%CI, 13,911-23,560; n=47) in BNT162b2-control (76.3-fold), 13,980 (11,902-16,421; n=118) in BNT162b2-extended (15.9-fold) and 10,799 (8,510-13,704; n=43) in AZD1222-extended (57.2-fold) participants. BNT162b2-control participants (median:262 days) had a longer interval between primary and booster doses than BNT162b2-extended or AZD1222-extended (both median:186 days) participants. Conclusions and Relevance: We observed rapid serological responses to boosting with BNT162b2, irrespective of vaccine type or schedule used for primary immunisation, with higher post-booster responses with longer interval between primary immunisation and boosting. Boosters will not only provide additional protection for those at highest risk of severe COVID-19 but also prevent infection and, therefore, interrupt transmission, thereby reducing infections rates in the population. Ongoing surveillance will be important for monitoring the duration of protection after the booster.

Corn silage as a source of aflatoxin B1 in feed for dairy cattle

2021 ◽  
Vol 26 (4) ◽  
pp. 2759-2764
Author(s):  
DRAGAN GLAMOČIĆ ◽  
MIROSLAVA POLOVINSKI HORVATOVIĆ ◽  
IGOR JAJIĆ ◽  
SAŠA KRSTOVIĆ ◽  
MIRKO IVKOVIĆ ◽  
...  
Keyword(s):  
Moisture Content ◽  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Corn Silage ◽  
Aflatoxin M1 ◽  
Fresh Matter ◽  
Whole Plant ◽  
Corn Grain

Nutrition of dairy cattle is based on two components, concentrates and forages. The main forages in Vojvodina, north province of Serbia is silage made from the whole plant of corn. After the outbreak of aflatoxin B1 in corn in 2012, the occurrence of aflatoxin B1 in corn as a source of contamination of aflatoxin M1 in milk was very broadly investigated. There is no data regarding the occurrence of aflatoxin B1 in silage and how much silage can contribute to the overall intake of aflatoxin B1 in this region. This work is an attempt to estimate how much silage, in condition and practice used in Vojvodina, contributes to the intake of aflatoxin B1, and consequently aflatoxin M1 in milk. In total, 82 samples of corn grain and 72 samples of corn silage were analyzed on the occurrence of aflatoxin B1 during 2017-2018 period. Aflatoxin B1 was found in 13.41% of corn samples in the range from 6.82 to 187.5 ppb (average 63.5 ppb). All positive samples were from 2017, while no positive samples were found during 2018. Incidence of aflatoxin B1 in silage was 54.17% in the range of 3.5-58.0 ppb (12% moisture content) or 0.95-16.1 ppb in the fresh matter. Results suggest that silage can be a significant factor to overall intake of aflatoxin B1 and that further research is needed.

Aflatoxin Conversion by Dairy Cattle Consuming Naturally-Contaminated Whole Cottonseed1

1985 ◽  
Vol 48 (1) ◽  
pp. 11-15 ◽  
Author(s):  
RALPH L. PRICE ◽  
J. H. PAULSON ◽  
OTIS G. LOUGH ◽  
CONRAD GINGG ◽  
ANDY G. KURTZ
Keyword(s):  
Steady State ◽  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Bulk Tank ◽  
Whole Cottonseed

Whole cottonseed determined to have aflatoxin B1 (AFB1) levels of 5, 31, 104, 280, and 560 μg/kg was fed as 15% of the total dairy ration to a commercial herd of 90 grade Holstein dairy cattle for 70 d. Milk from the bulk tank was sampled either daily or after each milking and analyzed for aflatoxin M1 (AFM1). The ratio for AFB1 in the dairy ration to AFM1 in the milk averaged 75 to 1 under conditions and at levels tested with no consistent relation to the level of AFB1 in the feed. Approximately 1.6% conversion occurred during the steady state of consumption and secretion. The federal action level of 0.5 μg AFM1/L of milk would be produced by cows consuming a ration containing 15% whole cottonseed contaminated with approximately 250 μg AFB1/kg.

Occurrence of aflatoxin B1 in diets of dairy cattle and aflatoxin M1 in raw milk

2012 ◽  
Vol 211 ◽  
pp. S51-S52 ◽  
Author(s):  
Ortiz-Martinez Raul ◽  
Arturo Valdivia-Flores ◽  
MA. Carolina de Luna-Lopez ◽  
Teodulo Quezada-Tristan ◽  
Armando Martinez-de Anda
Keyword(s):  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Raw Milk ◽  
Aflatoxin M1

scholarly journals Occurrence of aflatoxin B1, ochratoxin A and zearalenone in maize silage in the region of Vojvodina, Serbia

2019 ◽  
Vol 69 (1) ◽  
pp. 106-115 ◽  
Author(s):  
Dragan Glamočić ◽  
Miroslava Polovinski Horvatović ◽  
Igor Jajić ◽  
Saša Krstović ◽  
Darko Guljaš
Keyword(s):  
Dairy Cattle ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Daily Intake ◽  
Average Concentration ◽  
Aflatoxin M1 ◽  
Maize Silage ◽  
Limit Of Quantification ◽  
The Balkans ◽  
Two Samples

Abstract Silage made from the whole-plant maize is one of the most popular forages in Serbia. Consumption of maize silage by cows can be up to 30-35 kg/day. In Serbia in the few last years in the focus of the public and agriculture community were two mycotoxins, aflatoxin B1 and its metabolite aflatoxin M1 due to the outbreak of contaminated maize which affected the Balkans in 2012. Maize is regularly checked on the occurrence of aflatoxin B1, however forages are often neglected as a potential source of mycotoxins in the nutrition of dairy cattle. In this work, 48 samples of maize silage were analyzed for the occurrence of aflatoxin B1, ochratoxin A and zearalenone. Samples were collected from three regions (Bačka, Banat and Srem) in Vojvodina. In all samples, at least one mycotoxin above the limit of quantification was measured. Aflatoxin B1 was detected in 36 (75%) samples. In two samples from Banat, the concentration of aflatoxin B1 exceeded the maximum level (ML) set by Serbian regulation (30 µg/kg at moisture content of 12%). In seven samples, the concentration of aflatoxin B1 was above 20 µg/kg which is the EU regulated ML. Average concentration of ochratoxin A was 10.4 µg/kg, while the maximum measured value was 34.3 µg/kg. Maximum zearalenone content in all samples was 538 µg/kg while the average zearalenone concentration was 138 µg/kg. The results from this research point out that mycotoxin contaminated silage in the region of Vojvodina, Serbia can significantly contribute to daily intake of aflatoxin B1 in dairy cattle.

scholarly journals Application of Immunoaffinity Column Cleanup to Aflatoxin M1 Determination and Survey in Cheese

1996 ◽  
Vol 59 (9) ◽  
pp. 1011-1013 ◽  
Author(s):  
S. DRAGACCI ◽  
J. M. FREMY
Keyword(s):  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Acceptable Level ◽  
Aflatoxin M1 ◽  
Immunoaffinity Column ◽  
Cattle Feed ◽  
Milk Products ◽  
Immunoaffinity Columns

Milk products such as cheeses may be contaminated by aflatoxin M1 when manufactured with milk from dairy cattle that have consumed aflatoxin B1-contaminated feeds. The usefulness of immunoaffinity columns to determine aflatoxin M1 content in many kinds of cheeses with very good recoveries is demonstrated. The analysis of aflatoxin M1 in a 1990 to 1995 limited survey in France shows that the occurrence of this mycotoxin in cheeses is rather infrequent. With the exception of samples from 1989 to 1990 when aflatoxin B1-contaminated maize meals were incidentally imported to supplement dairy cattle feed, very few samples were found with above 0.200 μg of aflatoxin M1 per kg of cheese, the maximum acceptable level.

scholarly journals Seasonal milk contamination by aflatoxin m1, organophosphates and carbamates in Paraná – Brazil

2016 ◽  
Vol 37 (4) ◽  
pp. 2145
Author(s):  
Carlos Eduardo Crispim de Oliveira Ramos ◽  
Julio Cesar Damasceno ◽  
Ricardo Kazama ◽  
Thállitha Samih Wischral Jayme Vieira ◽  
Maximiliane Alavarse Zambom ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Production Systems ◽  
Colorimetric Method ◽  
Aflatoxin Contamination ◽  
Food Sources ◽  
Aflatoxin M1 ◽  
Milk Contamination ◽  
Chemical Residues ◽  
Contaminated Food ◽  
Layer Chromatography

Aiming to evaluate the milk contamination in the dairy production systems (DPS) for mycotoxins and chemical residues of organophosphates and carbamates it was made a study encompassing 96 DPS in three regions of Parana state. There were collected samples of milk, water and food and they were evaluated for chemical residues in all samples and aflatoxin only for food and milk. Mycotoxins in food (aflatoxin B1, B2, G1, G2, zearalenone and ochratoxin) were detected by the method of thin layer chromatography – TLC and for the determination of aflatoxin M1 was used an immunoassay kit competitive ELISA Ridascreen®. The residues of organophosphates and carbamates were performed by colorimetric method qualitatively. There were evaluated the differences between regions, periods and the sources of mycotoxin contamination. Carbamates and organophosphates were screened for their presence in milk and the sources of food and water. Then it was estimated the contributions of each mycotoxin for milk contamination, as well as their respective contaminated food. Differences were found between periods (p &lt; 0,05) for milk contamination with aflatoxin M1 – AFM1. For carbamates and organophosphates were found different contamination sources (p &lt; 0,01). For the carbamates the source were pesticides used to parasitic herd control and for the organophosphates pesticides used in agriculture. For food sources contamination resulting in the AFM1 contamination it was detected that aflatoxin B1 – AFB1 was the main source. The aflatoxin G1 – AFG1 showed a strong correlation (p &lt; 0,01) with AFB1 levels suggesting causal relationship is a function of fungal strains producing both at the same time. It was also found the prevalence of aflatoxin contamination in 70% of contaminated samples and its predominant presence in relation to other mycotoxins in all kinds of foods analyzed. By identifying the checkpoints of contamination can be proposed the inclusion of practical management methods to avoid this.

scholarly journals Comparative immune responses of pups following modified live virus vaccinations against canine parvovirus

2019 ◽  
Vol 12 (9) ◽  
pp. 1422-1427
Author(s):  
Jayalakshmi Vasu ◽  
Mouttou Vivek Srinivas ◽  
Prabhakar Xavier Antony ◽  
Jacob Thanislass ◽  
Vijayalakshmi Padmanaban ◽  
...  
Keyword(s):  
Immune Responses ◽  
Antibody Titer ◽  
Neutralizing Antibodies ◽  
Geometric Mean ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Canine Parvovirus ◽  
Serum Samples ◽  
Live Virus ◽  
Antibody Titers

Background and Aim: Canine parvovirus (CPV) is the most important viral cause of enteritis and mortality in pups. Evaluation and monitoring of pre- and post-vaccine immune responses may help to determine the efficacy of the current vaccination schedule being followed in pups in India. This study aimed to evaluate and monitor the pre- and post-vaccine immune responses of CPV vaccinated pups using hemagglutination inhibition (HI) assay. The neutralizing antibody titer levels were also detected using serum neutralization test (SNT). Materials and Methods: The pups were categorized into two groups, the double booster and the single booster groups. In this study, serum samples were subjected to HI and SNT for measuring the CPV antibody titer at frequent intervals for up to 6 months from 27 healthy pups following primary and booster CPV vaccinations. Results: The antibody titers in double booster pups reached their peaks at the 21st day after the second booster vaccination with a geometric mean (GM) of 3.57. The antibody titers in single booster pups reached their peaks at the 21st day after the first booster vaccination with a lower GM of 3.18. Conclusion: The double booster pups maintained a higher immune response throughout the period of the study compared to single booster pups though the difference in titers was not statistically significant. SNT results indicated that the raised antibody titer was also able to yield virus-neutralizing antibodies. No interfering maternally derived antibodies were found in the pups at the age of primary vaccination (45th day) in our study. Therefore, the second booster vaccination may be useful in maintaining the protective titer for a prolonged period.

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