Effects of Extraction Temperature and Time on Antioxidant Activities of Rhus verniciflua Extract

2013 ◽  
Vol 42 (11) ◽  
pp. 1776-1782 ◽  
Author(s):  
Hye Jin Park ◽  
Gun Mook Yoon ◽  
Sang Hoon Lee ◽  
Gwi Yeong Jang ◽  
Min Young Kim ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Extraction Temperature ◽  
Rhus Verniciflua

Ultrasonic-Assisted Aqueous Two-Phase Extraction Combined with Macroporous Resin Enrichment of Lignans from Flaxseed Meal and Their Antioxidant Activities

2021 ◽  
Author(s):  
Zhizhou Zhang ◽  
Yongchao Zhang ◽  
Yichao Wu ◽  
Yongxiang Xu ◽  
Yuanyuan Jiang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Phase System ◽  
Extraction Temperature ◽  
Macroporous Resin ◽  
Two Phase ◽  
Solid Ratio ◽  
Flaxseed Meal ◽  
Extraction And Purification ◽  
Ultrasonic Assisted

Abstract Background Secoisolariciresinol di glucoside (SDG) is a natural antioxidant generally extracted from flaxseed, which is one of the most important oil crops in China, the by-product of the flaxseed oil, i.e., flaxseed meal, still contains a lot of lignans. However, flaxseed meal is generally treated as waste, resulting in a huge waste of resources. Objective To establish an efficient and convenient method for extraction and purification of lignans from flaxseed meal. Methods First, we used response surface methodology (RSM) to optimize the extraction conditions of the ultrasonic-assisted aqueous two-phase system (UAATPS), and we obtained the purified extracts by macroporous resin purification (MRP). Second, the antioxidant ability of the extracts was studied in vitro. Results The best extraction conditions obtained were as follows: 9.0 wt% sodium hydroxide, 30.0 wt% isopropanol, extraction time 39 min, liquid-to-solid ratio of 52.0 mL/g, ultrasonic wave 560 W, and extraction temperature 40 °C. Under the optimal conditions, the purity of crude extracts (SDG-APTS-C) reached 21.5%. The desorption conditions of MRP were as follows: eluting 3 BV with ultrapure water, and then eluting with 25% ethanol at 2 BV/h to collect eluents. The purified extracts (SDG-ATPS-P) had a purity quotient of 73.9%, 52.4% higher than that of SDG-ATPS-C. Additionally, experiments conducted in this paper revealed that SDG-ATPS-C and SDG-ATPS-P could effectively remove DPPH, ABTS, and hydroxyl free radicals in vitro. Conclusions The method was validated for extracting SDG from flaxseed meal, thus achieving the reuse of flaxseed meal. Highlights This research provides some references for the application of UAATPS combined with MRP in natural products.

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

scholarly journals Total phenolic content and antioxidant activity of sesame cake aqueous extracts

2018 ◽  
Vol 17 (06) ◽  
pp. 77-84
Author(s):  
Tuyen C. Kha
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Major Influence ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Aqueous Extracts ◽  
Sesame Cake

The objective of this study was to investigate the efficiency of phenolic extraction and antioxidant activity from sesame cake using water extraction method and to evaluate the possibility of employing microwave irradiation to improve the extraction yield. The result showed that extraction temperature had major influence on total phenolic content and antioxidant activities of the extracts, whereas extraction time was found to be insignificant. The optimum extraction condition recommended were 90oC for 30 min in this research. Furthermore, microwave pre-treatment at 120 s could have significantly positive influence on the overall extraction yield, especially the total phenolics and antioxidants based on FRAP assay. Therefore, the obtained results suggest that sesame aqueous extracts could be a source of antioxidants with more feasible applications in food as well as other industries.

scholarly journals Effects of Time, Temperature, and Solvent Ratio on the Extraction of Non-Extractable Polyphenols with Anticancer Activity of Barhi Date Palm Kernels Extracts Using Response Surface Methodology

2019 ◽  
Author(s):  
Israa Mahmud ◽  
Mohamed E. S. Mirghani ◽  
Faridah Yusof ◽  
Ma'an Al-khatib
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Date Palm ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Dietary Polyphenols ◽  
Experimental Values

Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.

scholarly journals Essential Oil Extracted from Cymbopogon citronella Leaves by Supercritical Carbon Dioxide: Antioxidant and Antimicrobial Activities

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Hong Wu ◽  
Jilie Li ◽  
Yuan Jia ◽  
Zhihong Xiao ◽  
Peiwang Li ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Oil Quality ◽  
Antimicrobial Activities ◽  
Oil Yield ◽  
Chemical Compositions ◽  
Extraction Temperature ◽  
Experimental Conditions ◽  
Essential Oil Yield

To improve essential oil quality, especially to reserve the thermal instability of compounds, supercritical CO2 extraction (SFE) was applied to recover essential oil from Cymbopogon citronella leaves. A response surface methodology was applied to optimize the extraction process. The highest essential oil yield was predicted at extraction time 120  min, extraction pressure 25  MPa, extraction temperature 35°C, and CO2 flow 18  L/h for the SFE processing. Under these experimental conditions, the mean essential oil yield is 4.40%. In addition, the chemical compositions of SFE were compared with those obtained by hydrodistillation extraction (HD). There were 41 compounds obtained of SFE, while 35 compounds of HD. Alcohols and aldehydes were the main compositions in the essential oils. Furthermore, the antioxidant activities and antimicrobial of essential oils obtained by HD and the evaluated condition of SFE were compared. Results showed that the antioxidant activities of SFE oil are better than those of HD. Minimum inhibitory concentrations (MICs) were determined by the microdilution method. Essential oil obtained from SFE and HD exhibited a significant antimicrobial activity against all tested microorganisms. It is confirmed that the SFE method can be an alternative processing method to extract essential oils from Cymbopogon citronella leaves.

scholarly journals Optimization, antioxidant activity and bile salts adsorption capacity of the aqueous enzymatic extract from rice bran

2018 ◽  
Vol 36 (No. 4) ◽  
pp. 333-343
Author(s):  
Wang Yuxin ◽  
Yu Guoping ◽  
Zang Xiaodan ◽  
Ye Fei
Keyword(s):  
Rice Bran ◽  
Antioxidant Activities ◽  
Sodium Taurocholate ◽  
Value Added ◽  
Sodium Deoxycholate ◽  
Extraction Temperature ◽  
High Concentration ◽  
Enzymatic Extract ◽  
Antioxidant Power ◽  
Solid Ratio

The optimum extraction conditions of the aqueous enzymatic extract from rice bran (AEERB) were evaluated with respect to γ-oryzanol content and ferric reducing/antioxidant power (FRAP) activity by using response surface methodology. The results showed that the effect of the extraction temperature on γ-oryzanol content and FRAP activity was the most significant. The optimal conditions were determined as follows: Trypsin, 453 U/g at 56°C and pH 7.9 for 2 h at a liquid/solid ratio of 7 : 1. The corresponding γ-oryzanol content and FRAP activity were 0.1749 mg/g and 0.5043 mmol FeSO<sub>4</sub>/g, respectively. The antioxidant activities of AEERB were comparable with 2,6-di-tert-buty-4-methylphenol (BHT) or ascorbic acid (AA) at the high concentration. Furthermore, the binding capacities of AEERB to sodium taurocholate and sodium deoxycholate were 24.96 and 13.63 µmol/100 mg, respectively. The results indicated that AEERB is a promising method for developing and applying a value-added RB product.

Phenolic extracts from Rhus verniciflua Stokes bark by decompressing inner ebullition and their antioxidant activities

2014 ◽  
Vol 28 (7) ◽  
pp. 496-499 ◽  
Author(s):  
Hongxia Chen ◽  
Chengzhang Wang ◽  
Jianzhong Ye ◽  
Hao Zhou ◽  
Jiaojiao Yuan
Keyword(s):  
Antioxidant Activities ◽  
Rhus Verniciflua Stokes ◽  
Rhus Verniciflua ◽  
Phenolic Extracts

scholarly journals Optimization of Phenolic Compound Extraction from Chinese Moringa oleifera Leaves and Antioxidant Activities

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Beibei Zhao ◽  
Jiawen Deng ◽  
Hua Li ◽  
Yaqiang He ◽  
Tao Lan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ultrasonic Extraction ◽  
Extraction Temperature ◽  
Moringa Oleifera Leaves

Rich in phenolic compounds, Moringa oleifera leaf extract (ME) exhibits significant antioxidant activity both in vitro and in vivo. ME has already been widely used in fields of medicine, functional food, and cosmetics. Ultrasonic extraction (UE) method has been improved to be one of the most effective ways to extract phenols from M. oleifera leaves. The purpose of this study was to optimize ultrasonic extraction of phenols by response surface methodology (RSM). Four parameters were discussed, such as ethanol concentration, solvent-sample ratio, extraction temperature, and extraction time. Also, purification methods of the crude ME by organic solvent extraction and column chromatography were examined. Antioxidant activities of ME and each fraction were evaluated by DPPH, ABTS, and hydroxy radical-scavenging activities and reducing power. The phenol content of the purified ME reached up to 962.6 mg RE/g, extremely higher than the crude extract 107.22 ± 1.93 mg RE/g. The antioxidant activity of the purified ME was also significantly improved. Furthermore, phenols were identified by using the HPLC-MS method, and the results showed that there were 6 phenolic acids and derivatives and 7 flavonoids in ME. Quercetin-3-O-β-D-glucoside isolated from ME showed excellent DPPH and ABTS radical-scavenging abilities, which were comparable to VC.

scholarly journals Effect of extraction temperature and time on the extraction of phenolic compounds and antioxidant capacity of Phaleria macrocarpa fruit

2018 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Peng Yee Lim ◽  
Sook Fun Pang ◽  
Mashitah Mohd Yusoff ◽  
Jolius Gimbun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Pearson Correlation Coefficient ◽  
Flavonoid Content ◽  
Phaleria Macrocarpa ◽  
Optimum Extraction

Phaleria macrocarpa (ver. name: ‘mahkota dewa’) is a plant which has many medically useful antioxidant activities (Anggraini & Lewandowsky, 2015). The polyphenols responsible for this antioxidant activity has to be extracted before it can be routinely used (Shwter et al., 2016). This study investigates the extraction of polyphenols from P. macrocarpa fruits and its antioxidant activity (DPPH-RSA) under influence of extraction time and temperature. By employing maceration technique, the P. macrocarpa fruits extract showed the maximum total phenolic content (TPC), total flavonoid content (TFC) and DPPH-RSA with value of 69.5 mg QE/g DW, 183.2 mg GA/g DW and 171. 8 mg BHA/g DW, respectively at  optimum extraction conditions of 60 min and 80 ºC (Fig. 1). Excellent and positive Pearson correlation coefficient with R2 > 0.91 between the TPC, TFC and antioxidant activities was observed.

scholarly journals Effects of phenolic antioxidants extraction from four selected seaweeds obtained from Sabah

2015 ◽  
Author(s):  
Carmen Wai Foong Fu ◽  
Chun Wai Ho ◽  
Wilson Thau Lym Yong ◽  
Faridah Abas ◽  
Chin Ping Tan
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Antioxidant Compounds ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Single Factor ◽  
Sargassum Polycystum ◽  
Antioxidant Capacities

Algal have attracted attention from biomedical scientists as they are a valuable natural source of secondary metabolites that exhibit antioxidant activities. In this study, single-factor experiments were conducted to investigate the best extraction conditions (ethanol concentration, solid-to-solvent ratio, extraction temperature and extraction time) in extracting antioxidant compounds and capacities from four species of seaweeds (Sargassum polycystum, Eucheuma denticulatum , Kappaphycus alvarezzi variance Buaya and Kappaphycus alvarezzi variance Giant) from Sabah. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used to determine the phenolic and flavonoid concentrations, respectively, while 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picylhydrazyl (DPPH) radical scavenging capacity assays were used to evaluate the antioxidant capacities of all seaweed extracts. Results showed that extraction parameters had significant effect (p < 0.05) on the antioxidant compounds and antioxidant capacities of seaweed. Sargassum polycystum portrayed the most antioxidant compounds (37.41 ± 0.01 mg GAE/g DW and 4.54 ± 0.02 mg CE/g DW) and capacities (2.00 ± 0.01 µmol TEAC/g DW and 0.84 ± 0.01 µmol TEAC/g DW) amongst four species of seaweed. Single-factor experiments were proven as an effective tool to determine and quantify the relationship between a single factor and a single response variable.

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