scholarly journals Kisspeptin and Spexin: lipid profile and sperm parameters in infertile and fertile male subjects

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Muhammad Hasan Raza Raja ◽  
Mussarat Ashraf ◽  
Sher Khan ◽  
Nida Farooqui ◽  
Arfa Azhar ◽  
...  

BACKGROUND & OBJECTIVE: Kisspeptin (KP) and spexin (SPX) are neuropeptides that play a substantial role in metabolism and sexual function. Our objective is to explore the association of spexin and kisspeptin with sperm parameters and lipid profile in the infertile male population. METHODOLOGY: The cross-sectional study was conducted from March 2017 till July 2018 in Aga Khan University Hospital, Karachi, Pakistan, after acquiring ethical approval (4813-BBS-ERC-17). Convenient sampling was applied to recruit 44 normozoospermic subjects as controls and 44 patients with abnormal sperm parameters”. Serum levels of kisspeptin and spexin were measured by enzyme linked immunosorbent assay and Cobas c111 analyzer was used for estimation of high and low density-lipoprotein, triglyceride and serum cholesterol levels. Mann-Whitney U tests and spearman’s rank correlation test were used to analyze quantitative variables, and data was presented by means, standard error (SE) and correlations coefficient, with a statistical significance set at p≤0.05. RESULTS: The mean kisspeptin values of ‘altered sperm parameters’ males 13.23 ± 3.34 were lower than normal sperm parameters 31.89 ± 3.25 (p<0.001). The mean spexin values of altered sperm parameters 383.57 ± 21.09 were less than compared to normal sperm parameters 502.76 ± 19.77 (p<0.001). Correlation of KP and SPX levels was (r-value = 0.332, p= 0.002). There was no significant correlation was found between KP and SPX with lipid profile parameters. CONCLUSION: Spexin and kisspeptin levels observed were higher in male subjects with normal sperm parameters and had a significant positive correlation with each other. There was no impact of spexin on lipid profile and obesity.

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 923.4-924
Author(s):  
H. Hachfi ◽  
D. Khalifa ◽  
M. Brahem ◽  
N. Ben Chekaya ◽  
M. Younes

Background:Knee osteoarthritis and obesity are both major health problems. It is now admitted that the prevalence of knee osteoarthritis gets higher with obesity and that weight loss helps knee function and allows patients to avoid surgery.Objectives:The aim of this study was to study the influence of obesity on knee osteoarthritis features.Methods:A cross-sectional study was conducted in the university hospital Taher Sfar of Tunisia over a period of 6 months. Patients who had knee osteoarthritis confirmed by radiographs were included. Sociodemographic, clinical, radiological and therapeutic data were collected from medical records and visits. Obesity was defined by a body mass index (BMI) ≥30. Functional impairment was assessed by the Womac index and Lequesne index.Results:The study included 186 patients. There were 31 males and 155 femmes. The mean age was 60±10 years. The percentage of obese patients was 53,8%. The mean age was similar in both groups obese and non obese. There were more women in the obese group compared to the non obese group (p=0.0001), more patients who had diabetes mellitus and dyslipidemia (p=0.002). Non-obese patients had a shorter duration of symptoms with no statistical significance (p=0.151). Obese patients had more involvement of both knees (p<0.0001). Obesity did not have an impact on pain severity. Severity of radiological images (p=0,0001) were more frequent in obese patients. Functional impairment was similar in both groups. However, the percentage of patients having a very important functional impairment with Lequesne index was higher in obese patients (p<0.029). Obese patients also needed more physical therapy sessions (p=0.035).Conclusion:Knee osteoarthritis in obese patients is characterized with the femlae gender predominance, bilateral knee involvement, and a more severe images on radiographs. Thus the need for better control of weight and the importance of physical activity.References:[1]Coggon D, Reading I, Croft P, et al. Knee osteoarthritis and obesity. Int J Obes Relat Metab Disord J Int Assoc Study Obes 2001; 25: 622–627.Disclosure of Interests:None declared


2015 ◽  
Vol 22 (12) ◽  
pp. 1555-1559
Author(s):  
Mashooq Ali Dasti ◽  
Syed Fasih Ahmed Hashmi ◽  
Nisar Ahmed Shah ◽  
Syed Saad Hussain ◽  
Munaza Gohar ◽  
...  

Objectives: To determine the frequency of hyperuricemia in patients withessential hypertension. Study Design: Cross sectional descriptive. Period: Six months study.Setting: Liaquat University Hospital Hyderabad. Patients and Methods: All the patients withessential hypertension visited at cardiac OPD / admitted in the ward were further evaluated forserum uric acid level. The data was analyzed in SPSS 16 and the frequency and percentage wascalculated. Results: During six months study period, total one hundred and eighty (180) patientswith essential hypertension were recruited and study for uric acid level. The mean age ±SDfor overall population was 52.84±8.72 whereas it was 55.83±7.93 and 50.75±8.95 in male andfemale population respectively. The mean ± SD of systolic and diastolic blood pressure (mmHg)in overall population was 160.50 ± 12.74 and 100.70±5.95 respectively. The mean ±SD serumuric acid level in overall population was 13.74±4.83 while it was 11.74±6.44 and 14.43±4.31 inmale and female population respectively). The male population was predominant in relation toage (p=0.02), the hyperuricemia was identified in 117/180 (65%) patients and it is statisticallysignificant in context to age (p<0.01) and gender (p<0.05) whereas mean ±SD of systolic anddiastolic blood pressure was also significant in relation to hyperuricemia. Conclusion: There isa relationship between hyperuricemia and hypertension and shown that the serum uric acid levelwas significantly increased in patients with essential hypertension.


2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Roya Abbasi Natajomrani ◽  
Durdi Qujeq ◽  
Reza Hajihosseini ◽  
Vahid Hosseini

Background: Lipids are usually crucial to develop tumors, and dyslipidemia is correlated with the high chance of colon and colorectal cancer (CRC). Steroid hormones such as estrogen and progesterone can decrease the risk of colorectal cancer development. Objectives: The present study aimed to compare the serum levels of lipid profile and steroid hormones in patients with CRC and healthy controls. Methods: The present study included 40 consecutive adult patients with CRC in the Mazandaran Cancer Center, Sari, Iran, between 2017 and 2020.The diagnosis of CRC was evaluated based on colonoscopy with biopsy and CT scan. Also, the diagnosis of CRC was based on NCCN clinical practice guidelines in oncology. Blood samples were taken before treatment during routine testing. A 5 mL of peripheral blood was collected from each patient. All patients signed the written consent for the study. Also, a total of 40 healthy subjects were selected as healthy controls from the same area during a routine physical examination, which was also confirmed by screening colonoscopy and pathology. Serum TCh and TG levels were quantitatively determined by the colorimetric method. LDL-C and HDL-C were determined by the turbidimetric immunoassay. Steroid hormones were quantitatively determined by the Enzyme-linked immunosorbent assay (ELISA) according to the reagent manufacturer’s instruction. To analyze data, the SPSS software package (version 21) was applied. Results: Among all the indicators studied, the (mean ± SD) of testosterone, FSH and LH levels was higher (1.85 ± 1.63 ng/ml, 15.35 ± 0.13mIU/l, 12.42 ± 0.12.16mIU/ml) in patients with CRC than (mean ± SD) healthy controls (0.40 ± 0.21ng/ml, 6.27 ± 0.50mIU/ml, 2.89 ± 0.20mIU/ml, P < 0.05), respectively. Also, the results in subgroups showed that the mean testosterone (0.91 ± 1.2ng/l), FSH (19.11 ± 16mIU/ml), LH (14.49 ± 14mIU/ml) levels in the woman patients with CRC was higher than healthy female controls and had more statistical significance (p = 0.02, 0.00, 0.00), respectively. The area under the AUC cure (Figure 1) of the testosterone, FSH, and LH indicates positive test (0.670, 0.726 and 0.775). Conclusions: Changes in the levels of steroid hormones and lipids could correlate with the elevated chance of CRC. Therefore, assessment of multiple markers might overcome and provide better judgment in patients with CRC.


2015 ◽  
Vol 22 (03) ◽  
pp. 293-298
Author(s):  
Shuaib Ansari ◽  
Irfan Murtaza Shahwani ◽  
Zeeshan Ali ◽  
Syed Zulfiquar Ali Shah ◽  
Faisal Shahab

Systemic inflammatory response syndrome (SIRS) is a generalized disorder,immune response to infection which results in disturbed microcirculation, visceral perfusion andultimately visceral failure. Objectives: To determine the frequency of systemic inflammatoryresponse syndrome in patients with liver cirrhosis. Design: Cross sectional descriptive.Period: Six months study. Setting: At Liaquat University Hospital Hyderabad. Patients andmethods: All the patients with liver cirrhosis for 06 months duration admitted in the ward werefurther evaluated for systemic inflammatory response syndrome. The data was analyzed inSPSS 16 and the frequency and percentage was calculated. Results: During six months studyperiod, total 100 cirrhotic subjects were studied for SIRS. The mean ± SD for age in all (100)cirrhotic patients was 45.74 ± 7.537. The mean temperatures was 40.42±0.32 where as it was39.72±0.43 and 38.92±0.11 in male and female population respectively. The mean heart beatwas 128.42±6.74 where as it was 115.83±8.93 and 120.62±5.53 in male and female populationrespectively. The mean respiratory rate was 25.31±3.52 where as it was 23.52±2.31 and26.63±3.21 in male and female population respectively. The mean white blood cell count was28.81±4.51 where as it was 23.74±4.73 and 30.83±5.73 respectively. The SIRS was observedin 70% subjects of which 47(67.1%) were males and 23(32.9%) were females (p=0.04). Majorityof SIRS subjects were 30-39 years of age and male population was predominant (p=0.03). Thegender distribution in relation to severity of liver disease was statistically significant (p=0.05)while the SIRS in relation statical analysis. Conclusions: The systemic inflammatory responsesyndrome occurs in patients with liver cirrhosis.


2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Basma Sultan ◽  
Hamdy Omar ◽  
Housseini Ahmed ◽  
Mahmoud Elprince ◽  
Osama Anter adly ◽  
...  

Abstract Background and Aims Vascular calcification (VC) plays a major role in cardiovascular disease (CVD), which is one of the main causes of mortality in patients with chronic kidney disease (CKD). The study aims at early detection of breast arterial calcification (BAC) in different stages of CKD (stage 2, 3& 4) patients as an indicator of systemic VC. Method A case control study was conducted targeting CKD women, aged 18- 60 years old. The sample was divided into 3 groups; A,B,C (representing stage 2, 3 & 4 of CKD) from women who attended nephrology and Internal medicine clinics and admitted in inpatient ward in Suez Canal University Hospital. A 4th group (D) was formed as a control group and included women with normal kidney functions (each group (A, B, C, D) include 22 women). The selected participants were subjected to history taking, mammogram to detect BAC and biochemical assessment of lipid profile, Serum creatinine (Cr), Mg, P, Ca, PTH and FGF23. Results Our study detected presence of BAC in about 81.8% of hypertensive stage 4 CKD patients compared with 50% in stage 3 CKD, also in the majority of stage 4 CKD patients who had abnormal lipid profile parameters and electrolyte disturbance. Most of the variables had statistical significance regarding the presence of BAC. Conclusion Although it is difficult to determine the definite stage at which the risk of VC begins but in our study, it began late in stage 2 CKD, gradually increased prevalence through stage 3 and became significantly higher in stage 4. These results suggest that preventive strategies may need to begin as early as stage 2 CKD.


2021 ◽  
Vol 9 ◽  
pp. 251513552110158
Author(s):  
Abdoulreza Esteghamati ◽  
Shirin Sayyahfar ◽  
Yousef Alimohamadi ◽  
Sarvenaz Salahi ◽  
Mahmood Faramarzi

Background: Whole-cell pertussis (wP) vaccine administration is still advocated for children under 7 years of age in Iran. However, there is no recommendation for the administration of a dose of tetanus, diphtheria, and acellular pertussis (Tdap) vaccine to childbearing age/pregnant women in the Iranian vaccination program and it has increased the risk of infection through waning immunity during women’s childbearing age life. The study aimed to assess the levels of anti- Bordetella pertussis antibodies in childbearing age women of different ages in Iran. Methods: A cross-sectional study was conducted on a total number of 360 childbearing age women divided into six age groups, with 5-year intervals from 15 to 45 years old, in 2018–2019. Then, the levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) antibodies against B. pertussis were evaluated using enzyme-linked immunosorbent assay (ELISA). The IBM SPSS Statistics software (version 16.0) (SPSS Inc., Chicago, IL, USA) was used for data analysis. Results: The mean age of the participants was 30.01 ± 8.35 years (range 14–45 years). All the cases were IgM negative, but two IgA-positive individuals (in the age groups of 14–19 and 30–34 years) were reported. Overall, 239 (66.4%) cases were IgG positive. The mean age of IgG-positive cases was 30.37 ± 8.37 years. The IgG-positive cases were mostly in the age groups of 30–34 and 35–39 years [43 (71.1%)]. The odds of IgG positivity were 1.97. The highest odds of IgG positivity were seen in 30–34 and 35–39 years groups (2.52) and the lowest odds were seen in the 20–24 and 25–29 years groups (1.60). Using the Jonckheere–Terpstra test, the increasing trend of IgG changes in different age groups was not statistically significant (Tπ=5.78, p = 0.09). Conclusion: The infants of women of childbearing age might be prone to pertussis in countries using the wP vaccination schedule. It is suggested to administer a dose of Tdap to women before or during pregnancy to increase the immunity of their infants against this disease during early infancy.


Urolithiasis ◽  
2021 ◽  
Author(s):  
R. A. Kingma ◽  
M. J. H. Voskamp ◽  
B. H. J. Doornweerd ◽  
I. J. de Jong ◽  
S. Roemeling

AbstractCone beam computed tomography (CBCT) provides multiplanar cross-sectional imaging and three-dimensional reconstructions and can be used intraoperatively in a hybrid operating room. In this study, we investigated the feasibility of using a CBCT-scanner for detecting residual stones during percutaneous nephrolithotomy (PCNL). Intraoperative CBCT-scans were made during PCNL procedures from November 2018 until March 2019 in a university hospital. At the point where the urologist would have otherwise ended the procedure, a CBCT-scan was made to image any residual fragments that could not be detected by either nephroscopy or conventional C-arm fluoroscopy. Residual fragments that were visualized on the CBCT-scan were attempted to be extracted additionally. To evaluate the effect of this additional extraction, each CBCT-scan was compared with a regular follow-up CT-scan that was made 4 weeks postoperatively. A total of 19 procedures were analyzed in this study. The mean duration of performing the CBCT-scan, including preparation and interpretation, was 8 min. Additional stone extraction, if applicable, had a mean duration of 11 min. The mean effective dose per CBCT-scan was 7.25 mSv. Additional extraction of residual fragments as imaged on the CBCT-scan occurred in nine procedures (47%). Of the follow-up CT-scans, 63% showed a stone-free status as compared to 47% of the intraoperative CBCT-scans. We conclude that the use of CBCT for the detection of residual stones in PCNL is meaningful, safe, and feasible.


Author(s):  
Maria Theresa Costa Ramos de Oliveira Patrial ◽  
Rogério Hamerschmidt ◽  
Jorge Eduardo Fouto Matias ◽  
Evaldo Dacheux de Macedo Filho ◽  
Bettina Carvalho

Abstract Introduction The study of larynx lesions is of great importance. More than 50% of people with vocal complaints have benign vocal fold alterations and some require surgery. Objective To determine which factors are related to surgical recurrence of benign laryngeal lesions and to determine which videolaryngoscopic diagnoses are related to the risk or protection of surgical relapses over time. Methods Observational, analytical, cross-sectional study with retrospective data collection of 1,383 surgeries in 1,301 patients. Results The mean age at the first surgery was 39.2 years old. The predominant gender was female (65.6%).There were 396 cases of polyps (25.34%), 349 cases of cysts (22.33%), 261 cases of Reinke edema (16.7%), 175 cases of nodules (11.2%), 153 cases of minor structural alterations (MSAs) (9.79%), 94 cases of leukoplakia (6.01%), 77 cases of granulomas (4.93%) and 58 cases of pseudocysts (3.71%). The age presented statistical significance with the risk of surgical relapses (p = 0.016). Male gender was associated with the greatest chance of relapse.Diagnosis of granuloma (p < 0.001) and of leukoplakia (p < 0.001) were associated with a higher risk of surgical recurrence. Cyst diagnosis was associated with protection (p = 0.015) in relation to the chance of further surgeries. Patients with associated lesions, bilaterals or voice professionals did not present a statistically significant association with surgical recurrence. Conclusion Age and gender are statistically related to the increase of surgical relapses. Leukoplakia and granuloma are associated with increased surgical relapses. Patients submitted to cyst microsurgery appear to be protected against surgical recurrence.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Sintayehu Wolka ◽  
Sahilu Assegid ◽  
Temesgen Tantu ◽  
Muluken Gunta ◽  
Bereket Duko

Background. Assessing maternal satisfaction on delivery service has significant public health importance to measure the quality of maternal and child care services in a country. Therefore, the objective of this study was to further investigate the determinants of maternal satisfaction on delivery service provided at the Woliata Sodo University Teaching and Referral Hospital, Ethiopia. Methods. An institutionally based cross-sectional study was employed at the Wolaita Sodo University Hospital, Ethiopia. All mothers who gave birth between March and May 2018 were included in the study. Data were collected through using a pretested and structured interviewer-administered questionnaire. Both bivariate and multivariable logistic regression analyses were performed. A P value of <0.05 was used to declare statistical significance. Result. A total of 398 delivered mothers were included in the study. The rate of maternal satisfaction on existing delivery care was found to be 67.3%. Being less educated (AOR=5.06, [2.22-11.53]), primigravida (AOR=3.59, [1.17-11.04]), planned and wanted pregnancy (AOR=2.74, [1.21-6.18]), having antenatal care follow-up for current pregnancy (AOR=4.48, [2.04-9.83]), ever used family planning service (AOR=3.83, [1.95-67.52]), labor duration of less than 6 hours (AOR=5.96, [2.61-13.57]), and spontaneous vaginal delivery (AOR=2.82, [1.07-7.42]) were factors significantly associated with maternal satisfaction. Conclusion. In this study setting, maternal satisfaction was lower compared to other studies. Unreserved effort should be considered for future interventions.


2015 ◽  
Vol 58 (3) ◽  
pp. 86-91 ◽  
Author(s):  
Helena Doležalová ◽  
Josef Zemek ◽  
Luboš Tuček

Introduction: Cellulitis remains a very serious disease even today. Mortality, which varied between 10–40%, has been reduced owing to the standard securing of airway patency and use of an appropriate surgical treatment approach. Materials and methods: A total of 195 patients were hospitalised for cellulitis at the University Hospital in Hradec Králové during 2007–2011. The following parameters were evaluated: age, gender, dependence of incidence of the disease on the season of the year, frequency of attacks of the particular areas and their clinical characteristics, aetiology of the inflammation, types of patient complaints, prevalence of current systemic diseases, results of microbiological and selected laboratory analyses, socio-economic status of the patients, and duration of patient stay at the hospital. Statistical analysis was performed by using Pearson’s correlation coefficient, the statistical significance level was p < 0.05. Results: The mean age of the patients was 39.8 years. The group of 195 patients included 108 (55%) males and 87 (45%) females. The mean time between the first symptoms of the disease and admission to the Department was 5 days. From among the 195 patients, 116 (59.5%) were working persons, 79 (40.5%) were non-working (children, students, unemployed persons, women on maternity leave, retired people). The odontogenic origin of the disease was verified in 173 (88.7%) patients. In total, 65 (33.3%) patients had no coinciding complicating systemic disease, 22 (11.3%) patients had diabetes mellitus. The most frequent symptom of cellulitis was painful swelling, found in 194 (99.5%) patients, followed by jaw contracture, found in 153 (78.5%) patients. Conclusion: The results are largely very similar to those of previous studies performed in other countries, except that we found no correlation between the prevalence of cellulitis and the socio-economic status, nor have we confirmed Klebsiella pneumoniae sp. as the cause of cellulitis in patients with diabetes mellitus.


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